LOCUS       sequence-407487-        8973 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, TCF3_JAK2 from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8973)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 8973)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_TCF3_JAK2
FEATURES             Location/Qualifiers
     source          1..8973
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3734..4444,4445..4447,4448..4450))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3776..3797)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4125..4144
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4384..4405
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4447..4456
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4532..4735)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4561..4581)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4736..5039
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5296..5340)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5525..5758
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6118..6235
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6727..6852
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6899..7156
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7158..7537
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7568..7672
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7673..7741,7742..8533)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7891..7910)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8704..8973,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagc
     1801 cggccatgtt gtcacgaatc tgatccacac gaagcgctaa gtcgcgaaag ctgggacgct
     1861 gattaacgtt attattccag cattcagtca taatcatata aatttcatct ggacaaccgt
     1921 ctgggcgtgg caaacggcca ttatttttca gcagctcgat cagatggaaa acgatcattt
     1981 gaccttgttt gtcgtttcca atcatacgca taaattcagc cggaggagat ttagactttt
     2041 cgatataggt aaaaagctca taaagtacaa ctccgaagct ccacacatcg gaagcaaccg
     2101 agaattttga ttcggtaagg gactcgggcg catacttctc ctcctccgag tgatcggcag
     2161 cagatgtgtt ttcctcgtcc tccttctctt cacgtttgat ctcgctagct gcagccgtcg
     2221 ctcccgcacg gccaagacca ctataagagt caggtgggcg gctaaggtcc ggcagggttc
     2281 ccggttgaga tggcagagcc gcatgattgt gcattaagga agtagatccg gctaatccgt
     2341 cttcgggatg tgacccgcca acaaggcctg cgtgacgacc accaagactc atgggtcccg
     2401 taaacccgct cgctaaggct ccgtgccctg gtaaaagtgt gtgcatgtcg ccggcagtcc
     2461 caacggcatg agaacgtaat acgtgaatag cttcgtctaa gtgatcttcg attttagact
     2521 gaagcccatg cagtcctcca tcatagctag gtgacagtgc ccctggtgcc ccagcacgcg
     2581 gccattgtga ggtgccagct aagccttggg gcgatccaac cggtgttgag gggctagatg
     2641 agaaattgtt agaggaatga tctggagaat agatgctagc cagtgccttg cctaaggcat
     2701 ccccagaaga acccgccgtc gtcccacgag atccaagcag actatcggca ccactaacag
     2761 ggggcgtgtg tgagctcacc cccccgtaag tcgctccggg tgcgctagaa aaacttgagg
     2821 cagaaggcag tcctccgtta acctcagctc catgcagttg ataccccatg cgctcgtgct
     2881 ggtgtaaacc tccgaacgtg gacgacgagc cggaagaccc aactggccct gaaccaggcg
     2941 gcaatggcag aggcgaactc cccccgccta acatcggacc aaacccggcc tgacctgggg
     3001 gcgaccataa ctcagcactg gggtgcaggg aaccatccgc cacgtagaat ggtgccgggt
     3061 aggtgctcga tggggtcttc gcactagggt aagcagtagc atcgcggcca tagtcctcac
     3121 ccgagctggg agggtacacg gaactgggca gccccggcgg tactttgcgc actttctttg
     3181 gttgggtatc aagcgaacca tcagctgcgc gacgacgact gcttccactg tacgacgggt
     3241 aatactgaga cgttcctttc atacctgagg gtgaaagagg accaggggaa tttaatgcca
     3301 gctcaccact caaaaatccc gcttgggtaa gaccacccac gccagcatcg cgcccaaaag
     3361 aggcataggc accacgctcg ccacttttcc caccaagtcc aggccctaaa aatgtgctgg
     3421 aagataaact actatgcgac tcagtgaaat gtgtgccttc cgaaaatgta cgggaaggat
     3481 caaaagacga agatgattgg tcgccactcc cccagctgcc agaggagggg cggtcttcta
     3541 aaccactacc accgaattgt gcaccggcta acgaagcggg gcgtcccttg ccattagtaa
     3601 ctggcagcgg aaacatcatg ctgaaatcaa gcaggtcaga taattcttta tccgtaccca
     3661 ccggcgccat acgctgaggt tggttcattc cccgggaagc ggccgcactt cccccggagc
     3721 ctccgcttcc gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     3781 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
     3841 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     3901 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
     3961 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
     4021 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     4081 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     4141 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     4201 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
     4261 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     4321 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     4381 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     4441 tgctcaccat ggtggcgacc ggtggatccc gggcccgcgg taccgtcgac tgcagaattc
     4501 ggttcactaa acgagcccgg ttcactaaac gagctctgct tatatagacc tcccaccgta
     4561 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     4621 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     4681 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     4741 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     4801 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     4861 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     4921 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     4981 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     5041 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     5101 cacgcgttcg aagcttgagc tcggtcccct cggggttggg aggtgggtct gaaacgataa
     5161 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
     5221 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
     5281 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
     5341 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
     5401 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
     5461 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
     5521 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
     5581 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
     5641 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
     5701 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
     5761 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
     5821 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
     5881 atctcctcct tatggtgttt tactaaactt ttccatgttc taatcctcat cctgtctact
     5941 tgccacacaa tcatcacctg ccatctgttt tccataatcc ctaatgatct ttgcttttct
     6001 tcttggcact acttttatgt cactattatc ttgtattact actgcccctt cacctttcca
     6061 gaggagcttt gctggtcctt tccaaagtgg atttctgctg tccctgtaat aaacccgaaa
     6121 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
     6181 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
     6241 gatgaatact gccatttgta ctgctgtctt aagatgttca gcctgatctc ttacctgtcc
     6301 tataattttc tttaattctt tattcataga ttctactact ccttgacttt ggggattgta
     6361 gggaatttcc aggtctgaag atcctgctgc ttgctgtgct tttttcttac ttttgttttg
     6421 ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac
     6481 acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag
     6541 ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagt
     6601 attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt
     6661 tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt
     6721 aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg
     6781 cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct
     6841 gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg
     6901 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
     6961 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
     7021 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
     7081 gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc
     7141 gcccaggcca cgcctcccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
     7201 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
     7261 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
     7321 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
     7381 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
     7441 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
     7501 attgattact attaataact agtcaataat caatgtcgac ccaggtggca cttttcgggg
     7561 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     7621 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     7681 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     7741 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     7801 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     7861 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     7921 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     7981 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     8041 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     8101 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     8161 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     8221 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     8281 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     8341 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     8401 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     8461 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     8521 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
     8581 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     8641 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     8701 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     8761 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     8821 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
     8881 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
     8941 tgctgccagt ggcgataagt cgtgtcttac cgg
//
