LOCUS       sequence-407490-        9411 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, SMARCA2_ZNF384 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9411)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9411)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_SMARCA2_ZNF384
FEATURES             Location/Qualifiers
     source          1..9411
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4172..4882,4883..4885,4886..4888))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4214..4235)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4563..4582
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4822..4843
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4885..4894
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4970..5173)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4999..5019)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5174..5477
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5734..5778)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5963..6196
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6556..6673
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7165..7290
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7337..7594
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7596..7975
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8006..8110
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8111..8179,8180..8971)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8329..8348)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9142..9411,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac tagctagacg
     1801 ccaggtgctc aacctgaatg gtgctggtcg tcacggtcag gcagatatct ttgtgatgtt
     1861 ccgcggtctt gtacggagtc aaatcaaaac tacattgcgg cggcgggttc gggttgctat
     1921 caccaccacc accaccctgt ggtgctgccc ccggtgactg aaagtgcggc gggggctgtt
     1981 gctgctgctg ctgctgctgc tgttgttgtt gttgttgctg ctgactcgcc tggctggctt
     2041 gggcttgcgc ctgcgcttgg gcttgcgcct gcgcctgggc ctgggcctgc gcttgcgctt
     2101 gagccaccgc cgcagcagcc gccgccgcct ggacttgttg ctgcaggtcc ggcgggttgt
     2161 gcttgcgcat atgtttcatc aggtacgtct cgctggtgta cgcgcgggag caaatggtac
     2221 aggtataaac ttttgcgtgt ttgacggtat gggtggacaa atgaacctcc agggacgccg
     2281 catcggtata cgcacggtgg cagttatgac atttaaacgg tttgtccttg ttgtgctgac
     2341 ggcggtggct ctgcaggttc gacaattggg taaatgcttt ctcgcagcca ggatgtgcgc
     2401 acttgtacgg acggtcgccc gtgtgaatac gggtatgttg ttgcaggtgg ctcagctgac
     2461 gaaagctctt ttcgcagaaa ttacagctgt acggtttagc gccagagtga atgcgaatat
     2521 gttgagccag ataggagcta ttggcgaagg tcttcgaaca gtgcgggcat ttgtgcggtt
     2581 tcgtctcggt atggctcttg ctgtggatct gcatctcgga tttgctatag aaggtcaggg
     2641 agcacatacg gcagcggtag gtcttgccat ctttctgatg gtcgtcgtcg tcctccgggg
     2701 acagaacgta cggatcattc atctccggca agccagattc cagcattcgc ttttttttac
     2761 gaccacgcgg tggtttcggc gccacggaac caccaccgcc accgccacct ccaccttcct
     2821 cagtcagcgt gctggccacc ttcttagaca ggtccggcac cacctgtagc gcttggctac
     2881 ccggcggcag tgcagacacg atcatcggag cgctgatggg gaaggtttgc gctgagctag
     2941 cggtggtaac caagctacca gacgggctgg tgataactaa acccggacct ctggattggc
     3001 tcccttcacg acgccaacgt tggctacaag aaacacccgc ggtcatcagg ccagttgacg
     3061 gcaccggaac aacggtaatg ttctgggtga cgctggcttg gctgtgaggt gtcagctgat
     3121 cagacttcga ttcggtatcc atactgatgc cggacggcag ggaaacactt gccgggacag
     3181 tgagcagggt cggatagtgt ggcggcgcca gaccgcaacc tttctcgggc agtaactggt
     3241 ccttcatctt gttaatgaac atggtatttt cgatctggcc ggacaccgtc ggaatgctcg
     3301 gccagaagta ggggttgctg ttgaagtggc tctcttccat acggccgcta ggacggttat
     3361 aattgaccag tgccggctgt tgttgttgct gggtctgcgg ttgtggcggc tgctgctgag
     3421 gctgttgctg ttgctgctgc tgctgctgct gttgttgttg ctgttgctgc tgttgttgct
     3481 gttgttgctg caaacccggg agggtgcgtt tgccctgaac cgcgagctgc agtgtttccg
     3541 gcagcggctg accgcgcgcc agcatcttgt acgccaaaat ctgggcacgc aactgatgga
     3601 gctgcacggg gctgaacggg ctcggaccac gatttggttg gctcatcgct tgcgggtcgc
     3661 ccgggataag agcgcctggt tgcgatggcg gcatttgtgg cggggtcgga ccgccaccag
     3721 acatcgggct ggaaacatgc tccggcgcgc ccagcgggct cgggtgcggg ctcatataac
     3781 cttgggaatg ctggtccatc gggctttgtg gcgggcccat gcccggatgc ggcggacgca
     3841 tgccggtgcc cttcatgctg ccgcagtgga tgtcttctac aataccttta tcgtggatac
     3901 catcgatcgg cttatgcatc tgatgcatgc cttcttgcgg aaaatcagtg gaacccatgg
     3961 ttggcatcgg gtgactgact gagggcgggc ccggcgacgg acccatcatg ctatgcacgc
     4021 tacccggaga cgggcccggg cccggcgacg gacccaggat cggtccgggg ctcgggcccg
     4081 gacctggaga cggacccggg tgcggcatag ctccgggatc tgttggagtt gacatagcgg
     4141 ccgcacttcc cccggagcct ccgcttccgc ccttgtacag ctcgtccatg ccgagagtga
     4201 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     4261 tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     4321 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     4381 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     4441 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     4501 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     4561 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     4621 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     4681 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     4741 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     4801 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     4861 tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gcccgcggta
     4921 ccgtcgactg cagaattcgg ttcactaaac gagcccggtt cactaaacga gctctgctta
     4981 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     5041 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     5101 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     5161 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     5221 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     5281 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     5341 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     5401 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     5461 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     5521 attactatta ataactagca cgcgttcgaa gcttgagctc ggtcccctcg gggttgggag
     5581 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
     5641 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
     5701 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
     5761 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
     5821 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
     5881 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
     5941 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag
     6001 gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc
     6061 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc
     6121 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
     6181 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
     6241 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
     6301 tctccaattg tccctcatat ctcctcctta tggtgtttta ctaaactttt ccatgttcta
     6361 atcctcatcc tgtctacttg ccacacaatc atcacctgcc atctgttttc cataatccct
     6421 aatgatcttt gcttttcttc ttggcactac ttttatgtca ctattatctt gtattactac
     6481 tgccccttca cctttccaga ggagctttgc tggtcctttc caaagtggat ttctgctgtc
     6541 cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
     6601 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
     6661 ttttctttta aaattgtgga tgaatactgc catttgtact gctgtcttaa gatgttcagc
     6721 ctgatctctt acctgtccta taattttctt taattcttta ttcatagatt ctactactcc
     6781 ttgactttgg ggattgtagg gaatttccag gtctgaagat cctgctgctt gctgtgcttt
     6841 tttcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     6901 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     6961 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     7021 tgtttctaac aggccagtat taactgcgaa tcgttctagc tccctgcttg cccatactat
     7081 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     7141 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     7201 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     7261 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     7321 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     7381 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     7441 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     7501 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc
     7561 tcgccactcc ccagtcccgc ccaggccacg cctcccatgg taatagcgat gactaatacg
     7621 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
     7681 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
     7741 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
     7801 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
     7861 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta
     7921 tgaactaatg accccgtaat tgattactat taataactag tcaataatca atgtcgaccc
     7981 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8041 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8101 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8161 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8221 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8281 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8341 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8401 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8461 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8521 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8581 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8641 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8701 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8761 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8821 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     8881 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     8941 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9001 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9061 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9121 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9181 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9241 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9301 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9361 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg g
//
