LOCUS       sequence-407496-       12543 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, ZC3HAV1_ABL2 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12543)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 12543)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_ZC3HAV1_ABL2
FEATURES             Location/Qualifiers
     source          1..12543
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(4065..4076)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(join(7304..8014,8015..8017,8018..8020))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7346..7367)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7695..7714
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7954..7975
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8017..8026
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8102..8305)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8131..8151)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8306..8609
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(8866..8910)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9095..9328
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9688..9805
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10297..10422
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10469..10726
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        10728..11107
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11138..11242
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11243..11311,11312..12103)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11461..11480)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12274..12543,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac tacctctgca
     1801 ccacatcact gatttcctgt acacatgaca ataagttatt aaggacaggg tttgtcccgg
     1861 gcacaccagc agctgctgaa gaaacctgta gctcctgcag gctgagttcc agtttgctca
     1921 cagcctctcg gaaggcaaat ttgttgcgag tttgagggat gcagtccaca tagcctgagc
     1981 agtagtcaag cagctggtgt ccagtgtcta ccagctggct gttgggcaca ggttccgtga
     2041 gtgcactgga cagtaggtca gcacattcca gcagggcctc tttgctgatt ttgtctgctg
     2101 agattttctc agcggcctgt ttggtttttc tcagagccac tttagtacct gctgtgccat
     2161 tggccatttt ggctggcgag atggaagatg tgggcagagg cacttgaggt ggaggcatca
     2221 ctggcctccc agctttccca ctgatgggca ctgcgcccag agctgccttc tttcctcctt
     2281 cctgtgtttc tgatgtggac tgtcctgcag ttagggcagt tggctcttct gtagggtctg
     2341 agcagatgga cggatgctgc agtagtctca tcactggtgg tgggggtggg gcacactttg
     2401 gttttacccg tcggggtcgg tccttgtctc cagaggatgt gacctgatgc tcagataaga
     2461 gcttgaattt attcccctga gagtctgtgc caatgagctg cacgtcagct ggagtgtgtt
     2521 tcagagtggg tgagataagg actggcactt tgtggttgtg agtggttggg aggacggggg
     2581 cagccttggc tggagaaggc cagcccggct gctctccatc ctctggaact ccagccatcc
     2641 caagtcgtgc cccaccattc ttctctttac ccttgggggc agctgccact ccagccactc
     2701 ccacccctgg agggtccttc tctgtaatgg ctagatcccc agagggtgtt ctgagaggaa
     2761 gagctgtggc tcctctgggc aataacttgg cttttggtct ctccctgctt ggagcagcac
     2821 tttcctctga tttttttgga agcatgtcat tggccctgtc cacattctct tctggctgag
     2881 aagaggtgga cactgtcctt tccagctgga gtttggacct ctggcagttc ctgggaaggg
     2941 tcattgccat cctatcctgc tctggaagcc ccgtgagttc gtatttctta tggggctgat
     3001 tctccatttc tcggaaggag ctgctgcgtt tggggggtgt aggagcattt ctcttcttca
     3061 tgaaggagct gaagaagccc cccttcctat ccctggtgaa gcatgtctct ttggcatctt
     3121 ccaagaggct gctgggtgac ttgtctcttt gctttcgagg cagtgctggg gatccactag
     3181 aggcctgtgc acctctgatg aaccctggtg ctaaactgga agcagaattt tctgtggcat
     3241 cttgtgcccc ttcaatgttc tccttgttct ccacctgttt cttcagtgtc cgagtcttgg
     3301 aaggaagtat aggtagccgg ggcaggtatg gaacaacaga tgacgaggag gcggctctcc
     3361 caagctcctc agctacctct tcagaaatgc tggagtcatg gaacatggtt tcaaaagctt
     3421 ggtgtgtttc agcaaaagag ggcctatcgg cagggctcca cttccagcat gctctcataa
     3481 gttcataaac cttagggggg catccctcag gctgttccat tcgatatcct ttttctagta
     3541 ggtcatagac ctgagacagg tcaatacctg gatatggtga cattccatag gtagcaattt
     3601 cccacaacaa taccccaaaa gcccagacgt cagatttaat tgagaaggta ttgtaggcaa
     3661 gactctctgg tgctgtccac ttaataggaa atttggctcc agcatgagca gtataagtgt
     3721 ctccagtcat caatctactt aagccaaagt cagccacttt taccacatgg ttttctccca
     3781 ctaggcagtt acgagctgca agatctctat ggatgaaatt cttcttctct aagtactcca
     3841 ttgcagaaga aatctgagtg gccatgtaga gcagcacaac tgcagtcacc tcttctcggt
     3901 tgcattctcg gaggtaatcc agcaaattcc cgtatggcat gtattcagtc acaatgtaaa
     3961 atggtggctc caaagtacac acacctaaaa gttgtaccag attaggatgc ttgatttcct
     4021 tcattactgc agcttctttc aggaattctt ctacctccat ggtatcttcc ttcaatgttt
     4081 tcacagcaac tgtaaggctg tatttcttcc agacgccaac gtaaacctct ccatactgac
     4141 cgcccccaag tttgtgcttc atggtaatat ctgttcgctc catttcccat ttgtcgtgga
     4201 tgggggacac accatagact gtaggcttat tacacttggg tgctgggtag tgtaatgttg
     4261 tcaccagccc atcagccact gtggagtgat ggtgtacaag ctctgccaag gtgctgaagc
     4321 ggctctcagc agtcacatac accttgccat ctgcagtggt attgatcctg tagtgataca
     4381 cacgtccctc gtacctgagc gagatggaca gctgcccagg gctactctca ctttctcgca
     4441 ccaggaagct gccattgatt agactgctga gcagatactc agctgcactg cgtgacacag
     4501 gtccatggta ccaggagtgt ttttccaggc tgttcactgg ggtgatgtag ttgcttggca
     4561 cccagccctg cccattctta gagcgaactt cactccactc accattctgg ttgtaaccaa
     4621 ggactcgtag cttttcacct ttagtgatgc tgagtgtgtt atcaccactt gctacaaaat
     4681 cataaagtgc aacgaagaga ttagggtcac tctcagtggc tccgagcaag ttctccttgg
     4741 agctccacct gatagcctca tttagtgcct ggggttcaac atcacaacca tagggacgat
     4801 gcaaagcttc ttttccgtat ttgttttcat gagtttcatg taggaaactg tcaaaattat
     4861 tcgaacagat agattccaca taggcacggc ttgtcgcata aaataggagt tttccttctt
     4921 ccttcatctg cgatttcttc catgtaaatt tatccaggag ctctgagttc tcgatcttct
     4981 ttatcttttc aatcttgaaa tttttcatgg aagctttaaa atgctcactt actctgacat
     5041 attctggatg taggtgatgg atctctgaca acttatattt ctttgaggat aggaagcaaa
     5101 agtcctcctg aggacgaaag gtcgcagtta aagacactga cgaggtcttt gctggctgat
     5161 ggtctggccc tctcttcatc tgctgcacat accactgagg cacaaatgtt ggtcttctga
     5221 tgacatcctt ttgagttttg gaagctatgt ttgtctgaat catcccttgg aaactcagct
     5281 catagttccg tgagcccgcc tgaaatggca caactcccct cggacaggat tgatagagag
     5341 actccaggta tgaagagtcg acgtttgaat ttttccgttt gtctttctct tctccatact
     5401 gaatccatgt gccagattca ttcttccaat accaaatcca tttggtggtg aagacagaat
     5461 tggctggctt ggtgacagaa gaaggagtgg agaggcgtcg gatgggaaag gaatcacaac
     5521 tcattacccg aaaattgatt gtataacttc ctacagaaca gaggtggatt ccggggttac
     5581 agtagccttt ctcgatcgtc tccatgtgct caaagtccgt ccaggtttta ccaataagca
     5641 tctgccaccg gtaaggcaga tggaagtgga ctttgctgca gctaccatta agcggacaac
     5701 ccttacacag atggtcaaga caaatttcct ctgagtcatg atcatccacc ctagaagatg
     5761 tggtacttgt gacatcagat aaagaatcgt taacaagtgc tactcttggg tcagcatcat
     5821 ctgcgattct gccagtggcc tggacatctc gggaagcagg tccagcatcc tgaatcctag
     5881 gtgatgatat ttctctgtga ccgctgctag tgcttttgta atttaaggat ctggtagaag
     5941 ttatatctgt ggccactcca tcagcattat tattaaaaag agggccaggc tggatgtcct
     6001 gagttccact tttgccattg atgatcctgt agtctgagga aagcaagcct gtgccctttc
     6061 tggtggtcac agcttcaggt gtttgcagag agccaagaga agagcgggcg gcaggtagcg
     6121 tgggagaaaa cacagtcttt ctcctggcgc cttggtcatt cgtccaggat gtgaggctct
     6181 tccagttggg ggctgatgtt gaattggaag caaggtaagt gctgcctgga tttccatgca
     6241 agaggtcctc ttgactgccg ttctctaaaa acctctggct tgtcccggcc tgacttgttc
     6301 ctccaagatc agtagccttg gacgagcctg agggaggccg agcgcgatcc tgactcccca
     6361 gatacgtgaa cttgcgggtg agatcgtcca caggcgcgtc ctccagggaa gccctgtggc
     6421 tgatctgatc tggactaggt gtgcaggacc tctcagcaga cgctgaagca gacgcaagaa
     6481 attcttggct gccctgaaag aaccgatctc tactcttgct tctagcccta tatgccatgt
     6541 ttctacgatg tgaagaagga gctctgggcc ctgggggatt cttctgcatg tgcttgctgt
     6601 tgcagatgtc ctggatgttc tggaccacgt cggggttcag cccgtgctcc ctcatgatgg
     6661 ccagcacctt tctgtccatc aggttatggg accggaggca gttgggaaaa cgacagttcc
     6721 ctcgggtgaa gtggtcacag atgtggagtc ttgaacacgg tggctgctgg ttacaaatct
     6781 gctgccgacc ctctccctta taacttttgc atatctcggg cataaaaaaa ggatcacttt
     6841 ggaggaggag cactgctaat tcctctttgt tcagtccaga gagttcgtga tttttcagga
     6901 ctttgaagtt ctcttctgag agaacctcat gagaatattt gcataaattc cgctcggact
     6961 gcgaatagtt gcaccggccc agcaagttga gtttgcagag atgcaggtta tcgcagggtc
     7021 tctggcagta cttgcgacgg cagacccggg ctcgagtggt ggccaccacc gatcgggtga
     7081 tcccggcctc gccgccggtc tccaacacca caaagcggtc gggcccggcc acctgcagca
     7141 cctcacagag ctgcggctca gacagcgcga tctcctggag cagcgcgtcc agggccatgc
     7201 ggcccccgtg ggcgcacagg attttggtga tgaagcagca cacctccggg tccgccattc
     7261 cccgggaagc ggccgcactt cccccggagc ctccgcttcc gcccttgtac agctcgtcca
     7321 tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct
     7381 cgttggggtc tttgctcagc ttggactggg tgctcaggta gtggttgtcg ggcagcagca
     7441 cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt
     7501 cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc ttgtcggcca
     7561 tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg atgttgccgt
     7621 cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact
     7681 tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga
     7741 cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc
     7801 ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag ggcacgggca
     7861 gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc
     7921 cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg
     7981 gcaccacccc ggtgaacagc tcctcgccct tgctcaccat ggtggcgacc ggtggatccc
     8041 gggcccgcgg taccgtcgac tgcagaattc ggttcactaa acgagcccgg ttcactaaac
     8101 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     8161 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     8221 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     8281 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     8341 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     8401 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     8461 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     8521 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     8581 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     8641 accccgtaat tgattactat taataactag cacgcgttcg aagcttgagc tcggtcccct
     8701 cggggttggg aggtgggtct gaaacgataa tggtgaatat ccctgcctaa ctctattcac
     8761 tatagaaagt acagcaaaaa ctattcttaa acctaccaag cctcctacta tcattatgaa
     8821 taattttata taccacagcc aatttgttat gttaaaccaa ttccacaaac ttgcccattt
     8881 atctaattcc aataattctt gttcattctt ttcttgctgg ttttgcgatt cttcaattaa
     8941 ggagtgtatt aagcttgtgt aattgttaat ttctctgtcc cactccatcc aggtcgtgtg
     9001 attccaaatc tgttccagag atttattact ccaactagca ttccaaggca cagcagtggt
     9061 gcaaatgagt tttccagagc aaccccaaat ccccaggagc tgttgatcct ttaggtatct
     9121 ttccacagcc aggattcttg cctggagctg cttgatgccc cagactgtga gttgcaacag
     9181 atgctgttgc gcctcaatag ccctcagcaa attgttctgc tgctgcacta taccagacaa
     9241 taattgtctg gcctgtaccg tcagcgtcat tgacgctgcg cccatagtgc ttcctgctgc
     9301 tcccaagaac ccaaggaaca aagctcctat tcccactgct cttttttctc tctgcaccac
     9361 tcttctcttt gccttggtgg gtgctactcc taatggttca atttttacta ctttatattt
     9421 atataattca cttctccaat tgtccctcat atctcctcct tatggtgttt tactaaactt
     9481 ttccatgttc taatcctcat cctgtctact tgccacacaa tcatcacctg ccatctgttt
     9541 tccataatcc ctaatgatct ttgcttttct tcttggcact acttttatgt cactattatc
     9601 ttgtattact actgcccctt cacctttcca gaggagcttt gctggtcctt tccaaagtgg
     9661 atttctgctg tccctgtaat aaacccgaaa attttgaatt tttgtaattt gtttttgtaa
     9721 ttctttagtt tgtatgtctg ttgctattat gtctactatt ctttcccctg cactgtaccc
     9781 cccaatcccc ccttttcttt taaaattgtg gatgaatact gccatttgta ctgctgtctt
     9841 aagatgttca gcctgatctc ttacctgtcc tataattttc tttaattctt tattcataga
     9901 ttctactact ccttgacttt ggggattgta gggaatttcc aggtctgaag atcctgctgc
     9961 ttgctgtgct tttttcttac ttttgttttg ctcttcctct atcttgtcta aagcttcctt
    10021 ggtgtctttt atctctatcc tttgatgcac acaatagagg gttgctactg tattatataa
    10081 tgatctaagt tcttctgatc ctgtctgaag ggatggttgt agctgtccca gtatttgtct
    10141 acagccttct gatgtttcta acaggccagt attaactgcg aatcgttcta gctccctgct
    10201 tgcccatact atatgtttta atttatattt tttctttccc cctggcctta accgaatttt
    10261 ttcccatcgc gatctaattc tcccccgctt aatactgacg ctctcgcacc catctctctc
    10321 cttctagcct ccgctagtca aaatttttgg cgtactcacc agtcgccgcc cctcgcctct
    10381 tgccgtgcgc gcttcagcaa gccgagtcct gcgtcgagag agctcctctg gtttcccttt
    10441 cgctttcaag tccctgttcg ggcgccactg ctagagattt tccacactga ctaaaagggt
    10501 ctgagggatc tctagttacc agagtcacac aacagacggg cacacactac ttgaagcact
    10561 caaggcaagc tttattgagg cttaagcagt gggttcccta gttagccaga gagctcccag
    10621 gctcagatct ggtctaacca gagagaccca gtacaggcaa aaagcagctg cttatatgca
    10681 ggatctgagg gctcgccact ccccagtccc gcccaggcca cgcctcccat ggtaatagcg
    10741 atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat gtactgggca
    10801 taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact tggcatatga
    10861 tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca ttgacgtcaa
    10921 tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt caatgggcgg
    10981 gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac gcggaactcc
    11041 atatatgggc tatgaactaa tgaccccgta attgattact attaataact agtcaataat
    11101 caatgtcgac ccaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
    11161 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
    11221 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
    11281 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
    11341 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
    11401 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
    11461 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
    11521 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
    11581 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
    11641 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
    11701 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
    11761 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
    11821 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
    11881 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
    11941 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
    12001 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
    12061 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
    12121 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
    12181 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
    12241 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
    12301 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
    12361 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
    12421 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
    12481 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
    12541 cgg
//
