LOCUS       sequence-407497-       11328 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, TM7SF3_CUX2 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11328)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 11328)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_TM7SF3_CUX2
FEATURES             Location/Qualifiers
     source          1..11328
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(4511..4530)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4576..4631
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(4630..4649)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4992..5008)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4992..5008)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5005..5027)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5016..5032
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5040..5046,5047..5064,5065..5070))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5085..5106
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        5165..5360
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5211..5346
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    5366..5500
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5403..5422)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5457..5476
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(5568..5585)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5739..6327)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5819..5838)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6498..7289,7290..7358))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7121..7140
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7359..7463)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        7494..7873
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             7875..8132
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8179..8304
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8796..8913
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9273..9506
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9691..9735
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        9992..10295
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10296..10499
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10450..10470
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      10575..10584
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(10581..10583,10584..10586,10587..11297)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(10626..10647)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(10887..10906)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     11234..11255
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccgcttcccg gatgggcttt ttacaactgc tggtggtcgc cgtcttggct agcgaacatc
       61 gcgttgcggg tgccgcagaa gtgtttggga attcatcaga ggacccggtc cccgtatttg
      121 aagcggctcg ctccttagac gatcgtcttc aaccacctag ttttgatccg tctggccagc
      181 cccgccgtga cctgcacact tcctggaaac gcaaccctga acttttaagt cccaaagaac
      241 aacgcgaagg tacatcgccg gctggcccaa cacttactga gggttcccgc ctgcctggta
      301 tccccggaaa agctctttta accgagaccc ttttacagcg caatgaagcg gagaagcaaa
      361 aagggttgca agaggtgcag atcactcttg ctgcccgcct gggtgaggct gaagagaaaa
      421 tcaaggtgct gcactcagca ttaaaggcta cacaagcgga gcttcttgaa ttgcgtcgta
      481 aatacgacga ggaagccgcg agcaaggctg acgaggtagg gcttattatg actaacctgg
      541 agaaagctaa tcagcgcgcg gaggcggcac aacgcgaggt ggagtcgtta cgcgagcagt
      601 tagcctctgt gaactcctcc attcgtttag cgtgttgttc accccagggg cccagtgggg
      661 ataaagtaaa ttttacttta tgttccggac cgcgcttgga ggctgcgtta gcatcaaaag
      721 atcgtgaaat cttgcgtttg ctgaaagatg ttcagcacct gcaaagcagt cttcaagagt
      781 tagaagaagc atcagctaac caaattgctg atctggagcg ccaattgacc gcgaaatcgg
      841 aggcgatcga gaaattggaa gagaaacttc aggcccaaag tgactatgaa gaaattaaga
      901 cagaactttc gattctgaag gccatgaaac tggcctcaag tacttgctca cttccacaag
      961 gtatggcaaa gcctgaggat tccttactga ttgctaagga ggccttcttt cctacccaga
     1021 agttcctttt ggagaaaccg tcacttttgg cttcaccaga agaagatcct agcgaggacg
     1081 attctattaa agattcgttg ggaaccgagc aatcctaccc atcccctcaa cagctgcctc
     1141 cacccccggg gcctgaagat cctttgtccc catcaccagg tcagccgctt ttgggcccta
     1201 gtttaggccc ggacgggact cgcacgttct ccctgagccc atttccgtct ttagctagtg
     1261 gcgaacgttt aatgatgcct cctgccgctt tcaaggggga ggcaggcgga cttcttgttt
     1321 ttccgccggc tttttatggt gcgaaacccc ctactgcacc agcgactcca gctcccggac
     1381 cagaaccatt gggcgggcct gaacccgcgg acgggggtgg tggtggggct gcgggccccg
     1441 gcgccgaaga ggaacaactg gatacggcgg agatcgcttt tcaagtaaag gaacaactgc
     1501 tgaagcataa cattggacag cgcgtttttg gacattacgt gttgggctta tcccagggct
     1561 ctgtgtccga gattcttgct cgcccaaagc cttggcgcaa acttaccgtg aagggtaagg
     1621 agccgttcat taagatgaag caattccttt cagacgaaca gaacgtactt gcacttcgca
     1681 caatccaagt ccgtcagcgc ggatctatta ccccacgtat tcgcactcct gagaccggta
     1741 gcgacgacgc aattaagagc atcttggaac aggcaaagaa ggagattgaa agccagaagg
     1801 ggggcgagcc taagacatcc gtggcgccat tgagcatcgc caacgggacc accccagcgt
     1861 ccaccagcga agatgcaatc aagtcgattt tagaacaagc ccgccgtgaa atgcaagcgc
     1921 aacagcaagc tttgctggag atggaggtgg ctcctcgtgg ccgttctgtg ccaccgtcgc
     1981 ctcctgagcg tccttcgttg gcgacagcat ctcaaaacgg tgcccccgcc ctggtcaagc
     2041 aagaagaagg ttcaggcgga cctgctcaag caccactgcc cgtcttgtca ccggcagcgt
     2101 tcgtacaaag cattattcgc aaagtaaagt cggaaatcgg tgacgccggc tattttgatc
     2161 accattgggc ctccgatcgt ggtcttcttt cacgccctta tgcgtctgtt agcccgtcac
     2221 ttagtagtag ctcaagttct gggtattcgg gtcaaccaaa cggacgtgca tggccgcgcg
     2281 gtgacgaagc tcctgtgcca cctgaagacg aggctgccgc tggcgctgag gatgagcctc
     2341 ctcgtaccgg agaattgaag gctgagggag cgaccgcgga ggcgggcgcc cgtcttcctt
     2401 actatccagc ttatgttcca cgtacgttga agccaacggt ccccccactt actcctgagc
     2461 agtacgagtt atacatgtat cgtgaggtgg acaccctgga attgacacgc caagttaaag
     2521 agaagttggc gaagaatggg atttgtcagc gcatctttgg cgagaaagta ttaggcttgt
     2581 cgcaggggtc ggtgtccgat atgctgtccc gccctaaacc ttggagtaag ttaactcaaa
     2641 agggacgtga acccttcatc cgtatgcagc tgtggttgtc tgaccaactt ggtcaggctg
     2701 ttgggcagca gccgggggcc tctcaggcct caccaactga accacgttca tcaccttcgc
     2761 cgcctccttc ccccactgag ccagaaaagt ctagtcagga gcctctgtcg ttgtcactgg
     2821 agagcagtaa agagaaccag cagccggaag gtcgtagctc ttcttcctta tcaggcaaaa
     2881 tgtactcagg aagtcaagcc ccaggaggaa ttcaagagat tgtcgctatg tcgccggaac
     2941 tggatactta ctcgattacg aaacgcgtca aggaggttct gacagataat aatttgggcc
     3001 agcgtttgtt cggcgaaagt atcctggggc tgacccaggg atcggtcagc gatttattat
     3061 cacgtcctaa accctggcac aagttaagtt taaaaggacg tgaacctttc gtacgtatgc
     3121 aattgtggtt aaacgatcca cacaatgttg agaaattacg cgacatgaaa aaattggaga
     3181 agaaggccta cttgaaacgt cgctatggat tgattagtac tgggagtgac agcgagagtc
     3241 ccgcaacccg ctctgaatgt ccttcgccct gcttacagcc tcaagactta tctttgttac
     3301 aaattaagaa accacgtgtc gtcttagcgc ccgaagagaa agaggccttg cgcaaggcat
     3361 atcagctgga gccatatccc tcgcaacaaa ccatcgaact gctttctttt cagttaaatt
     3421 tgaagaccaa cacagtaatc aactggtttc ataactaccg cagccgtatg cgtcgcgaaa
     3481 tgctggtaga agggacccaa gacgaaccgg atttggaccc ctccggtggc cccggaatcc
     3541 ttccacccgg tcacagccac ccagatccta ctccgcaaag ccccgatagc gagaccgagg
     3601 atcaaaaacc taccgtcaaa gaactggagt tgcaggaagg gccagaagaa aatagtacac
     3661 ctttgactac tcaggataaa gctcaagtcc gcattaagca agagcaaatg gaagaagatg
     3721 cggaggaaga agctggttcc caacctcaag acagtggcga attagacaaa ggtcagggcc
     3781 caccgaaaga ggagcatcct gatcctccag ggaatgatgg tcttcccaag gttgctccag
     3841 gtcctttatt acccggaggc tcaactcccg attgccctag cttacaccct cagcaagagt
     3901 cagaggccgg ggagcgtctg cacccagatc cgctgagttt taagtcggca tccgagtcca
     3961 gccgttgttc actggaggtc tcacttaatt caccgtctgc cgcgtcttct ccaggactta
     4021 tgatgtctgt ttcccctgtc ccctcgtcgt cagcccccat ctcaccaagc ccgccagggg
     4081 cccccccagc taaggtgcct tcggccagcc caacagccga catggctggc gcgttacacc
     4141 cttctgcgaa ggttaaccct aaccttcaac gtcgtcatga gaagatggca aacttaaata
     4201 acattatcta tcgtgttgag cgtgcggcaa accgtgagga ggcattagag tgggaattct
     4261 gaaccggttc tagataagat tacggtacct ttaagaccaa tgacttacaa ggcagctgta
     4321 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaaaga
     4381 agacaagatc tgctttttgc ctgtactggg tctctctggt tagaccagat ctgagcctgg
     4441 gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt cagctgctcg
     4501 agctagcaga tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc
     4561 atctgacttc tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt
     4621 tgtgtctctc actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt
     4681 atttggttta gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc
     4741 tataaagagg tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa
     4801 aagccttgac ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa
     4861 catccctaaa attttcctta catgttttac tagccagatt tttcctcctc tcctgactac
     4921 tcccagtcat agctgtccct cttctcttat ggagatccct cgacctgcag cccaagcttg
     4981 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac
     5041 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc
     5101 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagcgg
     5161 atccgcatct caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc
     5221 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     5281 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     5341 gaggcctagg cttttgcaaa aagctaactt gtttattgca gcttataatg gttacaaata
     5401 aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg
     5461 tttgtccaaa ctcatcaatg tatcttatca tgtctggatc cgctgcatta atgaatcggc
     5521 caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     5581 tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     5641 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     5701 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     5761 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     5821 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     5881 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5941 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     6001 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     6061 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     6121 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     6181 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     6241 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     6301 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     6361 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     6421 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     6481 taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     6541 ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     6601 ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     6661 gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     6721 ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     6781 gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     6841 tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     6901 atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     6961 gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     7021 tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     7081 atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     7141 agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     7201 ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     7261 tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     7321 aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     7381 tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     7441 aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg gtcgacattg
     7501 attattgact agttattaat agtaatcaat tacggggtca ttagttcata gcccatatat
     7561 ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc
     7621 ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag ggactttcca
     7681 ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac atcaagtgta
     7741 tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg cctggcatta
     7801 tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg tattagtcat
     7861 cgctattacc atgggaggcg tggcctgggc gggactgggg agtggcgagc cctcagatcc
     7921 tgcatataag cagctgcttt ttgcctgtac tgggtctctc tggttagacc agatctgagc
     7981 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     8041 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     8101 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     8161 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     8221 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     8281 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     8341 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     8401 agcagggagc tagaacgatt cgcagttaat actggcctgt tagaaacatc agaaggctgt
     8461 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     8521 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     8581 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaaaaa agcacagcaa
     8641 gcagcaggat cttcagacct ggaaattccc tacaatcccc aaagtcaagg agtagtagaa
     8701 tctatgaata aagaattaaa gaaaattata ggacaggtaa gagatcaggc tgaacatctt
     8761 aagacagcag tacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg
     8821 gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa
     8881 ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagaaat
     8941 ccactttgga aaggaccagc aaagctcctc tggaaaggtg aaggggcagt agtaatacaa
     9001 gataatagtg acataaaagt agtgccaaga agaaaagcaa agatcattag ggattatgga
     9061 aaacagatgg caggtgatga ttgtgtggca agtagacagg atgaggatta gaacatggaa
     9121 aagtttagta aaacaccata aggaggagat atgagggaca attggagaag tgaattatat
     9181 aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc aaagagaaga
     9241 gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg gttcttggga
     9301 gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc cagacaatta
     9361 ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc gcaacagcat
     9421 ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct ggctgtggaa
     9481 agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa actcatttgc
     9541 accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca gatttggaat
     9601 cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt aatacactcc
     9661 ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt ggaattagat
     9721 aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta tataaaatta
     9781 ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt actttctata
     9841 gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct cccaaccccg
     9901 aggggaccga gctcaagctt cgaacgcgtg ctagttatta atagtaatca attacggggt
     9961 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
    10021 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
    10081 taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc
    10141 acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac gtcaatgacg
    10201 gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt cctacttggc
    10261 agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacatca
    10321 atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca
    10381 atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt aacaactccg
    10441 ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc
    10501 gtttagtgaa ccgggctcgt ttagtgaacc gaattctgca gtcgacggta ccgcgggccc
    10561 gggatccacc ggtcgccacc atggtgagca agggcgagga gctgttcacc ggggtggtgc
    10621 ccatcctggt cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg
    10681 gcgagggcga tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc
    10741 tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc
    10801 gctaccccga ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg
    10861 tccaggagcg caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga
    10921 agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg
    10981 acggcaacat cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca
    11041 tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg
    11101 acggcagcgt gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg
    11161 tgctgctgcc cgacaaccac tacctgagca cccagtccaa gctgagcaaa gaccccaacg
    11221 agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc actctcggca
    11281 tggacgagct gtacaagggc ggaagcggag gctccggggg aagtgcgg
//
