LOCUS       sequence-407500-       14106 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, TRIO_TERT from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14106)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 14106)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_TRIO_TERT
FEATURES             Location/Qualifiers
     source          1..14106
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     14..33
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(252..356)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        387..766
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             768..1025
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1072..1197
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1689..1806
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2166..2399
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2584..2628
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2885..3188
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3189..3392
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3343..3363
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3468..3477
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3474..3476,3477..3479,3480..4190)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3519..3540)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3780..3799)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4127..4148
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(11510..11529)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    11575..11630
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(11629..11648)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(11991..12007)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(11991..12007)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12004..12026)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12015..12031
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12039..12045,12046..12063,12064..12069))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12084..12105
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        12164..12359
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12210..12345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    12365..12499
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(12402..12421)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     12456..12475
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(12567..12584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(12738..13326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12818..12837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(13497..14106,1..182,183..251))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
       61 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
      121 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
      181 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
      241 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
      301 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
      361 tttccccgaa aagtgccacc tgggtcgaca ttgattattg actagttatt aatagtaatc
      421 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt
      481 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta
      541 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg
      601 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga
      661 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt
      721 tcctacttgg cagtacatct acgtattagt catcgctatt accatgggag gcgtggcctg
      781 ggcgggactg gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg
      841 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      901 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      961 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     1021 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     1081 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     1141 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     1201 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     1261 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     1321 aatactggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     1381 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     1441 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     1501 gagcaaaaca aaagtaagaa aaaagcacag caagcagcag gatcttcaga cctggaaatt
     1561 ccctacaatc cccaaagtca aggagtagta gaatctatga ataaagaatt aaagaaaatt
     1621 ataggacagg taagagatca ggctgaacat cttaagacag cagtacaaat ggcagtattc
     1681 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     1741 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     1801 aattttcggg tttattacag ggacagcaga aatccacttt ggaaaggacc agcaaagctc
     1861 ctctggaaag gtgaaggggc agtagtaata caagataata gtgacataaa agtagtgcca
     1921 agaagaaaag caaagatcat tagggattat ggaaaacaga tggcaggtga tgattgtgtg
     1981 gcaagtagac aggatgagga ttagaacatg gaaaagttta gtaaaacacc ataaggagga
     2041 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     2101 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     2161 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     2221 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     2281 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     2341 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     2401 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     2461 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     2521 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     2581 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     2641 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     2701 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     2761 ccattatcgt ttcagaccca cctcccaacc ccgaggggac cgagctcaag cttcgaacgc
     2821 gtgctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     2881 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     2941 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     3001 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     3061 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     3121 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     3181 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
     3241 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
     3301 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
     3361 gtgtacggtg ggaggtctat ataagcagag ctcgtttagt gaaccgggct cgtttagtga
     3421 accgaattct gcagtcgacg gtaccgcggg cccgggatcc accggtcgcc accatggtga
     3481 gcaagggcga ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg
     3541 taaacggcca caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc
     3601 tgaccctgaa gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga
     3661 ccaccctgac ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg
     3721 acttcttcaa gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg
     3781 acgacggcaa ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc
     3841 gcatcgagct gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg
     3901 agtacaacta caacagccac aacgtctata tcatggccga caagcagaag aacggcatca
     3961 aggtgaactt caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact
     4021 accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga
     4081 gcacccagtc caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg
     4141 agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag ggcggaagcg
     4201 gaggctccgg gggaagtgcg gccgcttccc ggatgtctgg aagcagcggt ggcgcggcag
     4261 cacccgcagc gagttctggg ccagcggcgg cagcttcggc agcagggtct ggttgtggtg
     4321 gaggagctgg ggaaggagcc gaggaggcag cgaaggactt agccgacatc gctgcattct
     4381 ttcgctccgg cttccgcaag aacgacgaaa tgaaagcgat ggatgtactt ccaatcctta
     4441 aagaaaaggt tgcttactta tccggcggtc gcgacaagcg tggcggcccc atcttaacgt
     4501 tcccggcacg ttcaaatcac gaccgcattc gtcaggaaga cttacgtcgt ttaattagct
     4561 acttggcttg tattccgagt gaggaggtat gcaaacgcgg tttcaccgta attgtagata
     4621 tgcgcggttc caaatgggac tccattaaac cattacttaa aatcttacag gagtcatttc
     4681 cttgctgcat ccatgttgca cttatcatta aaccagacaa cttctggcag aaacaacgca
     4741 cgaattttgg tagcagtaaa tttgagttcg agacgaatat ggtatccctg gaaggtctta
     4801 cgaaagtggt tgatccgtcc caattaacgc ctgaatttga tgggtgtctt gagtataatc
     4861 atgaggagtg gattgagatc cgtgttgctt ttgaggacta catctcaaat gcgacgcaca
     4921 tgttaagtcg cttagaggaa ttacaagata ttttagcaaa gaaggagctg ccgcaagatt
     4981 tagagggagc ccgcaacatg attgaagaac actctcagtt aaaaaagaag gttatcaaag
     5041 cccctatcga agaccttgat cttgaggggc agaagttact gcaacgtatc cagtcgagcg
     5101 aatcttttcc aaaaaaaaat agcggttccg ggaatgccga tcttcaaaat ttgttgccta
     5161 aagtcagtac gatgttagac cgcttgcatt ccactcgcca acatttgcat cagatgtggc
     5221 acgtgcgcaa gctgaagctg gaccagtgtt tccagttgcg tcttttcgaa caagacgctg
     5281 agaagatgtt tgactggatt actcataaca aaggactttt tttaaatagc tacacggaaa
     5341 ttggaacctc ccacccacat gctatggaat tacaaacaca acacaaccac tttgcgatga
     5401 attgcatgaa tgtttatgtc aacattaacc gtatcatgtc agttgcgaac cgtcttgttg
     5461 aatctggcca ctacgcaagt caacagattc gccaaatcgc aagtcagctt gaacaggaat
     5521 ggaaggcgtt tgctgctgca ttagatgagc gcagcacgtt gcttgacatg tcctctatct
     5581 tccaccagaa agcggagaag tacatgtcaa acgtagatag ttggtgcaag gcatgtggcg
     5641 aggtggattt gccgtccgag ctgcaagact tggaagacgc tatccatcat catcagggca
     5701 tttatgagca catcaccctg gcttattcag aagtctccca agacggaaaa agtctgctgg
     5761 ataagttgca gcgtccgctg acgcccggtt ccagtgattc tctgacagca tcagctaatt
     5821 actctaaggc cgtccatcat gtgctggatg tcatccacga agtgttacac catcagcgtc
     5881 agttggagaa tatttggcag catcgtaagg tacgcttgca tcagcgcctt cagttgtgtg
     5941 tattccagca agacgtgcaa caggtccttg attggattga aaatcatggg gaagcgttct
     6001 tgtccaagca taccggagtt gggaagtctt tacatcgtgc acgtgcgtta caaaaacgcc
     6061 atgaagattt cgaggaggta gcgcagaaca cgtacaccaa tgcggacaaa ctgcttgagg
     6121 ctgcagaaca attagctcag actggggagt gtgatccaga ggagatttac caagcagccc
     6181 accagttgga ggatcgtatt caagatttcg tccgccgcgt tgagcagcgc aaaattttgt
     6241 tagatatgtc ggtttcattt catacgcatg ttaaggaatt atggacttgg ttagaagagt
     6301 tgcagaaaga actgttagac gatgtgtatg ctgaaagtgt agaagccgta caggatctga
     6361 ttaagcgttt tggccagcaa caacagacaa cgcttcaggt taccgtaaac gtaattaagg
     6421 aaggggaaga tctgattcag cagttacgtg actctgcgat ttcatccaat aagaccccgc
     6481 ataactccag catcaatcat attgagacgg tacttcagca gttggacgaa gcacagtccc
     6541 agatggaaga actgttccaa gagcgcaaaa ttaagcttga attatttctt cagttgcgca
     6601 tttttgagcg cgatgctatt gacatcatct cagatttaga aagctggaat gatgagcttt
     6661 cgcagcaaat gaacgatttt gatacagagg acctgacaat tgcagaacaa cgcttacagc
     6721 atcatgctga taaggcttta actatgaata acttgacgtt cgatgtcatt caccaagggc
     6781 aagatttgct tcagtatgtt aacgaggtgc aggcatccgg agtcgaatta ttgtgtgatc
     6841 gtgatgttga tatggcgaca cgcgtacagg acctgttaga gtttctgcat gagaaacaac
     6901 aggaactgga cttagccgca gagcagcacc gcaagcactt agaacaatgt gttcagctgc
     6961 gccacttaca agcggaggtt aaacaggttt tgggttggat tcgtaatggc gaatccatgt
     7021 taaatgcggg ccttattacg gcgtcgtccc tgcaggaggc tgaacaattg caacgtgagc
     7081 atgaacaatt ccagcacgct atcgaaaaga cgcaccagtc ggcattacaa gttcaacaaa
     7141 aggccgaagc gatgttgcaa gcaaatcatt acgacatgga tatgattcgc gattgtgcgg
     7201 aaaaggtcgc ctctcattgg cagcagctga tgttaaagat ggaggaccgt cttaaattgg
     7261 taaacgcttc cgtagcattt tacaagacgt cggaacaagt ctgctctgtc cttgaatccc
     7321 tggagcagga gtacaaacgt gaagaggatt ggtgcggcgg tgccgataaa ttgggtccga
     7381 actctgaaac agaccatgta accccaatga tttccaaaca tttagaacaa aaagaagcat
     7441 tccttaaagc atgtacatta gcccgccgca atgctgatgt tttcctgaaa taccttcacc
     7501 gtaattctgt taacatgccc ggaatggtga cccacatcaa ggcgcctgaa caacaggtga
     7561 agaatatctt aaacgaattg tttcaacgcg aaaaccgcgt ccttcattat tggaccatgc
     7621 gtaagcgccg cttagaccag tgtcagcaat acgttgtgtt cgagcgctct gccaaacagg
     7681 cgcttgaatg gattcacgac aacggggagt tttacttatc gacccatacc tcaaccggtt
     7741 ctagcatcca gcacactcag gagcttctta aggagcacga ggaatttcaa atcacggcaa
     7801 aacagactaa ggaacgcgtc aaattgttaa tccagttagc agacgggttt tgcgaaaaag
     7861 gacacgcgca tgctgcagag atcaaaaagt gtgtgaccgc cgtggataag cgttaccgcg
     7921 atttttcctt gcgtatggaa aaatatcgca catcacttga aaaggcctta ggaatctcta
     7981 gtgactctaa taagtctagc aagagtctgc agttagatat cattcctgcg tcgattcctg
     8041 gttcggaggt gaaattacgc gatgcagcac atgagcttaa tgaggaaaaa cgcaaaagcg
     8101 cccgccgtaa agagtttatt atggctgaat taatccagac tgagaaagcg tacgtccgcg
     8161 atctgcgcga gtgcatggat acgtatcttt gggagatgac ctccggggtc gaagagatcc
     8221 ctcctggtat tgttaacaag gaattaatta ttttcggtaa tatgcaggag atctatgaat
     8281 ttcacaataa tatttttctt aaagagttgg agaagtatga gcaactgccc gaagacgtag
     8341 ggcattgctt cgtaacgtgg gcggataagt ttcaaatgta tgtcacttat tgtaagaata
     8401 aacctgactc tacgcagctt atcctggagc atgctggttc gtatttcgat gagatccaac
     8461 aacgtcacgg gttggcgaac agtatttcga gttatttaat caagccagtt cagcgcatca
     8521 ctaaatatca attactgtta aaagaacttt taacttgttg cgaagagggt aagggcgaaa
     8581 tcaaagatgg gctggaagtt atgttgtctg ttccgaaacg cgccaatgat gcgatgcatt
     8641 tgtcgatgtt agagggcttc gatgagaata ttgagagcca gggagaattg attttgcagg
     8701 agtctttcca ggtttgggat ccgaagaccc tgattcgcaa gggacgtgag cgtcaccttt
     8761 ttctgtttga gatgtctctt gttttctcca aagaagttaa agactcctct ggccgctcta
     8821 aatatttgta taagtccaag ctgtttacat ctgagttggg agtaactgag cacgtagagg
     8881 gcgacccttg taaatttgct ttgtgggtcg ggcgtactcc gacatccgac aataagatcg
     8941 tattgaaggc ttcgagcatc gagaataaac aggactggat caagcatatt cgtgaagtca
     9001 ttcaggagcg tacaatccat ttaaagggcg ctcttaaaga accaattcac attccaaaga
     9061 ctgcgccggc gacgcgccaa aagggacgtc gtgacggtga agaccttgac agccagggcg
     9121 acggctccag tcagcctgac accattagca tcgcaagccg cacctcccag aatactttgg
     9181 atagtgacaa actgtccggt ggctgcgagt tgacagttgt tattcatgat tttaccgctt
     9241 gtaactctaa tgaactgaca attcgccgtg gacagacggt tgaggtgtta gagcgccctc
     9301 acgacaagcc tgattggtgc ttggtgcgta caactgaccg tagcccggca gcggagggcc
     9361 tggttccgtg cggcagttta tgcatcgcgc actcgcgctc ttcgatggaa atggaaggta
     9421 tctttaatca taaaggcgtt ggatgcgtcc ctgccgctga acatcgtttg cgtgaagaga
     9481 ttctggctaa attcttacat tggcttatgt ccgtttacgt cgtggagttg cttcgcagct
     9541 ttttctacgt gactgagacg acatttcaga agaatcgctt attcttctac cgtaaaagtg
     9601 tctggtccaa acttcagtcg attggcatcc gccaacattt aaagcgtgtt caattgcgcg
     9661 agttgagtga ggccgaagtg cgtcaacatc gcgaggcccg tcctgcattg ttaacgtcac
     9721 gcctgcgttt cattccaaag cccgacgggt tacgtccgat tgtcaatatg gattacgtcg
     9781 tgggagcacg tacttttcgc cgcgagaaac gcgcggaacg ccttacaagc cgtgttaagg
     9841 ccctgttttc tgtgttaaat tatgaacgtg cccgccgccc aggcttgttg ggagcctccg
     9901 ttttaggcct ggacgatatt caccgcgctt ggcgcacctt tgttcttcgt gtacgtgcgc
     9961 aagatcctcc tcccgagttg tactttgtga aagttgatgt tacgggcgcg tacgacacaa
    10021 tcccacaaga tcgtctgacg gaagttattg cgagtatcat taaaccccag aatacgtact
    10081 gcgtccgtcg ctatgccgta gtccaaaaag ctgcacatgg gcatgtacgt aaggcattca
    10141 agagtcatgt cagcacattg acggatttac agccttacat gcgccagttc gtcgctcatc
    10201 tgcaagaaac ctcacctttg cgtgacgccg ttgtgattga gcagtccagc agcctgaatg
    10261 aggcaagtag tggcttgttt gacgtcttcc tgcgctttat gtgccaccat gccgttcgta
    10321 ttcgtggtaa gtcctacgtg caatgtcaag gcatcccaca gggtagtatt ttgtccacac
    10381 tgttgtgcag cctgtgctat ggggatatgg aaaataagct gtttgcgggt atccgtcgcg
    10441 atggtcttct gttgcgcttg gtagatgact tccttttggt tactccacat ttaactcacg
    10501 ctaaaacttt cctgcgtaca ttagtccgcg gtgttccgga gtacgggtgt gtggttaatc
    10561 ttcgcaagac ggttgtcaat ttccctgtag aggacgaagc tctgggtgga accgcttttg
    10621 ttcagatgcc ggcacacgga ctgttcccct ggtgcgggct tcttcttgat acgcgcacgt
    10681 tggaagtaca atcggattat agctcttatg cgcgcacgtc gatccgtgct tctcttactt
    10741 tcaatcgtgg atttaaggct ggtcgcaaca tgcgccgtaa attgttcggt gtccttcgct
    10801 tgaagtgtca ctctttattc cttgatctgc aagtcaactc tttgcaaact gtgtgtacta
    10861 acatctacaa gattcttttg cttcaagcat atcgctttca cgcgtgcgtt ctgcaacttc
    10921 catttcatca gcaagtttgg aaaaatccga cctttttcct gcgtgtcatc tctgatacag
    10981 caagcctgtg ttactcaatt cttaaggcga aaaatgccgg aatgtcgttg ggggccaagg
    11041 gggcagctgg acctttacct agcgaggccg tccaatggct gtgccaccaa gcatttttac
    11101 tgaaattgac tcgtcaccgt gttacatacg tgcccttatt agggagcctg cgtaccgccc
    11161 aaacgcaact gagtcgcaag ctgccaggaa ccaccttaac cgctcttgag gcggcagcta
    11221 acccggcgct gccttctgat tttaaaacca ttttagattg aaccggttct agataagatt
    11281 acggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa
    11341 gaaaaggggg gactggaagg gctaattcac tcccaaagaa gacaagatct gctttttgcc
    11401 tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
    11461 aacccactgc ttaagcctca ataaagcttc agctgctcga gctagcagat ctttttccct
    11521 ctgccaaaaa ttatggggac atcatgaagc cccttgagca tctgacttct ggctaataaa
    11581 ggaaatttat tttcattgca atagtgtgtt ggaatttttt gtgtctctca ctcggaagga
    11641 catatgggag ggcaaatcat ttaaaacatc agaatgagta tttggtttag agtttggcaa
    11701 catatgccca tatgctggct gccatgaaca aaggttggct ataaagaggt catcagtata
    11761 tgaaacagcc ccctgctgtc cattccttat tccatagaaa agccttgact tgaggttaga
    11821 ttttttttat attttgtttt gtgttatttt tttctttaac atccctaaaa ttttccttac
    11881 atgttttact agccagattt ttcctcctct cctgactact cccagtcata gctgtccctc
    11941 ttctcttatg gagatccctc gacctgcagc ccaagcttgg cgtaatcatg gtcatagctg
    12001 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
    12061 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
    12121 ctgcccgctt tccagtcggg aaacctgtcg tgccagcgga tccgcatctc aattagtcag
    12181 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
    12241 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
    12301 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
    12361 agctaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
    12421 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
    12481 atcttatcat gtctggatcc gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
    12541 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
    12601 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
    12661 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
    12721 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
    12781 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
    12841 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
    12901 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
    12961 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
    13021 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
    13081 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
    13141 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
    13201 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
    13261 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
    13321 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
    13381 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
    13441 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
    13501 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
    13561 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
    13621 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
    13681 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
    13741 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
    13801 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
    13861 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
    13921 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
    13981 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
    14041 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    14101 tgcccg
//
