LOCUS       sequence-407509-       10260 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, EML4_ALK from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10260)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 10260)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_EML4_ALK
FEATURES             Location/Qualifiers
     source          1..10260
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(5021..5731,5732..5734,5735..5737))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5063..5084)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5412..5431
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5671..5692
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5734..5743
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5819..6022)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5848..5868)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        6023..6326
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6583..6627)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6812..7045
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7405..7522
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    8014..8139
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             8186..8443
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8445..8824
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8855..8959
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8960..9028,9029..9820)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9178..9197)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9991..10260,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagg
     1801 gcccaggctg gttcatgcta ttcttgcttt tcagaatggt atcctcgtaa tgaccagctc
     1861 caggggcagt agcggcttct aagggcaagc cctgttgctg gtagccgtaa ttgacattcc
     1921 cacaagggaa gtgacgtagc ctgaacagag gtacctcctt catattggca gtcagcgaag
     1981 agggctctag gagcagtgag gcccccggaa gtctcccagt tgcaacgtta ggtgggacag
     2041 tacagcttcc ctccagcccc aggttacccc tgtcgtgtgg ctccttcttt gctataggat
     2101 tattcttttt ggtgggtttc tctgtaaacc aggagccgta cgttgggttc cacaagctgg
     2161 tgggcttgtt tctggatccg tggaccttgt gcaactccga aggagggttg gactgagaga
     2221 atgccatatt cacgtgtccc ccttccacgg ccggccctct agggactcga acagagatct
     2281 ctgcagctgt gggtttcttt gcagccttgc cagaggaggt ggtaggcaga ggtggtgggg
     2341 cagctgggct gcgctcctcc tcccgttttg cctgttgaga gaccaggaga ggaggaaccc
     2401 cctcagggtc cttgggcctc acaggcactt tctcttcctc ttccacaagt ggaccatatt
     2461 ctatcggcaa agcggtgttg attacatccg ggtcctgggt gcagtattca atcctctcca
     2521 aaatgatggc aaagttgggc ctgtcttcag gctgatgttg ccagcactga gtcattatcc
     2581 ggtatacagg cccagggcag ttcttgggtg ggtccatccg gcctccactg gtgacaaact
     2641 ccagaacttc ctggttgctt ttgctggggt atggcatata tccaagagaa aagatttccc
     2701 atagcagcac tccaaaggac catgtgtctg ttttagaagt gaatattcct tccatgaagg
     2761 cctctggggg catccactta actggcagca tggcacagcc tccctttcta tagtagctcg
     2821 ccctgtagat gtctcgggcc atcccgaagt ctccaatctt ggccactctt ccagggcctg
     2881 gacaggtcaa gaggcagttt ctggcagcaa tgtctcggtg gatgaagtgg ttttcctcca
     2941 aatactgaca gccacaggca atgtcccgag ccacgtgcag aaggtccagc atggccaggg
     3001 aggagggctg gctcgggcga gggcgggtct ctcggaggaa ggacttgagg tctccccccg
     3061 ccatgagctc cagcaggatg aaccggggca gggattgcag gctcacccca atgcagcgaa
     3121 caatgttctg gtggttgaat ttgctgatga tcagggcttc catgaggaaa tccagttcgt
     3181 cctgttcaga gcacacttca ggcagcgtct tcacagccac ttgcaggggg cttgggtcgt
     3241 tgggcattcc ggacacctgg ccttcataca cctccccaaa ggcgccatgg cccagacccc
     3301 gaatgagggt gatgtttttc cgcggcacct ccttcaggtc actgatggag gaggtcttgc
     3361 cagcaaagca gtagttgggg ttgtagtcgg tcatgatggt cgaggtgcgg agcttgctca
     3421 gcttgtactc agggctctgc agctccatct gcatggcttg cagctcctgg tgcttccggc
     3481 ggtacacttt aggtcctttc ccaggtgtgg gctctacagt agttttgctc catataagca
     3541 tgactccacc tgagtctcca gtaagaacat ctccattccc caagaatgct aaacactgca
     3601 caaattttgg cttttcatat ttcccaaaaa ttccctgttt tcttgttagt gaattgccgc
     3661 tccaggtcca gaagaaaata tgagatttac cgcatgtaat tatggtattt gcatctgttg
     3721 ggtgaaactc cacagccaaa acaacttcat ttgttgtctt tatttctgct ccttttgctt
     3781 tcttctgcca gtcccataca gtaagcatat gctcattgga gtcatcaata acacataaat
     3841 gaacacctga atctgctttt gaaaaatcca ggcatcctac tccacgctca aaagtgccaa
     3901 gtccaataat ctgcagtgtg gatagagtaa cagaatccca cactctgacg tggggttgta
     3961 gaggccttcc atctttatcc acgccagcta tctgtccagt tgcaatccta attttgtcag
     4021 gatgtatagc aaggcatttc acacagtctg tatggcccag gtagtgtcgc tgagttctct
     4081 cctcataatt aaatagtact actactgatg caatgaaata aactatttcc ccggtcggaa
     4141 gaaggtaaac attagctcta cagtcctttc ctcgataacc atatgcccac tccagtttga
     4201 gcttctcagg aggcagttcc gttctgatgt catcatagtt gtcaacatcg gaaggaatga
     4261 acatggtaat tggccgaccg cgcataaaca ttttaatata ttctccttct tggttgatga
     4321 tgacatcttt atgcttgtct gcagttttgg taacttttgg tattaatttg ggtgttgaag
     4381 gtattttcga caatttatta cggctatcat ctgaattttc ccaagaatta tgtgactttt
     4441 cagctggtga tggtcgtttt atgcttttgg tgggagtagc attcttgctt tctggagttt
     4501 gtctgtgtat ttggagaggt tgtgaagagg gctggggaga aggtgatgct cgaatttgtg
     4561 gactttgatc attagaatga gattcctctt ttttttctct ctgtccttgt ggtttttctt
     4621 tctttttttc tgtaccactt ttagcagcag atgaaagagt ttcttttcct gcaattgaga
     4681 cagcactggt atgacttggt tttctgtttg caccacttcc attggttata caggacatgg
     4741 gaataactgc tcgagggctt ggttggcctt tacttgagac tgattttttc actgaggcca
     4801 catgatcttc agagattgca agacgcctca aaacatcagc caaagccgcc tttagcacag
     4861 tgatttcatc ttcttgttgc tgaactcgtg actcaagagc tgacaggcga tcttgaacat
     4921 cagaagtact tgcagcagaa atactatcat cgagactgcc ggcgaaaccg tccattcccc
     4981 gggaagcggc cgcacttccc ccggagcctc cgcttccgcc cttgtacagc tcgtccatgc
     5041 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
     5101 tggggtcttt gctcagcttg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
     5161 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
     5221 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
     5281 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
     5341 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
     5401 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
     5461 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
     5521 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
     5581 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
     5641 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
     5701 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcgaccggt ggatcccggg
     5761 cccgcggtac cgtcgactgc agaattcggt tcactaaacg agcccggttc actaaacgag
     5821 ctctgcttat atagacctcc caccgtacac gcctaccgcc catttgcgtc aatggggcgg
     5881 agttgttacg acattttgga aagtcccgtt gattttggtg ccaaaacaaa ctcccattga
     5941 cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa ccgctatcca cgcccattga
     6001 tgtactgcca aaaccgcatc accatggtaa tagcgatgac taatacgtag atgtactgcc
     6061 aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc catttaccgt
     6121 cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact gccaagtggg
     6181 cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt ggcgttacta
     6241 tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg
     6301 ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga actaatgacc
     6361 ccgtaattga ttactattaa taactagcac gcgttcgaag cttgagctcg gtcccctcgg
     6421 ggttgggagg tgggtctgaa acgataatgg tgaatatccc tgcctaactc tattcactat
     6481 agaaagtaca gcaaaaacta ttcttaaacc taccaagcct cctactatca ttatgaataa
     6541 ttttatatac cacagccaat ttgttatgtt aaaccaattc cacaaacttg cccatttatc
     6601 taattccaat aattcttgtt cattcttttc ttgctggttt tgcgattctt caattaagga
     6661 gtgtattaag cttgtgtaat tgttaatttc tctgtcccac tccatccagg tcgtgtgatt
     6721 ccaaatctgt tccagagatt tattactcca actagcattc caaggcacag cagtggtgca
     6781 aatgagtttt ccagagcaac cccaaatccc caggagctgt tgatccttta ggtatctttc
     6841 cacagccagg attcttgcct ggagctgctt gatgccccag actgtgagtt gcaacagatg
     6901 ctgttgcgcc tcaatagccc tcagcaaatt gttctgctgc tgcactatac cagacaataa
     6961 ttgtctggcc tgtaccgtca gcgtcattga cgctgcgccc atagtgcttc ctgctgctcc
     7021 caagaaccca aggaacaaag ctcctattcc cactgctctt ttttctctct gcaccactct
     7081 tctctttgcc ttggtgggtg ctactcctaa tggttcaatt tttactactt tatatttata
     7141 taattcactt ctccaattgt ccctcatatc tcctccttat ggtgttttac taaacttttc
     7201 catgttctaa tcctcatcct gtctacttgc cacacaatca tcacctgcca tctgttttcc
     7261 ataatcccta atgatctttg cttttcttct tggcactact tttatgtcac tattatcttg
     7321 tattactact gccccttcac ctttccagag gagctttgct ggtcctttcc aaagtggatt
     7381 tctgctgtcc ctgtaataaa cccgaaaatt ttgaattttt gtaatttgtt tttgtaattc
     7441 tttagtttgt atgtctgttg ctattatgtc tactattctt tcccctgcac tgtacccccc
     7501 aatcccccct tttcttttaa aattgtggat gaatactgcc atttgtactg ctgtcttaag
     7561 atgttcagcc tgatctctta cctgtcctat aattttcttt aattctttat tcatagattc
     7621 tactactcct tgactttggg gattgtaggg aatttccagg tctgaagatc ctgctgcttg
     7681 ctgtgctttt ttcttacttt tgttttgctc ttcctctatc ttgtctaaag cttccttggt
     7741 gtcttttatc tctatccttt gatgcacaca atagagggtt gctactgtat tatataatga
     7801 tctaagttct tctgatcctg tctgaaggga tggttgtagc tgtcccagta tttgtctaca
     7861 gccttctgat gtttctaaca ggccagtatt aactgcgaat cgttctagct ccctgcttgc
     7921 ccatactata tgttttaatt tatatttttt ctttccccct ggccttaacc gaattttttc
     7981 ccatcgcgat ctaattctcc cccgcttaat actgacgctc tcgcacccat ctctctcctt
     8041 ctagcctccg ctagtcaaaa tttttggcgt actcaccagt cgccgcccct cgcctcttgc
     8101 cgtgcgcgct tcagcaagcc gagtcctgcg tcgagagagc tcctctggtt tccctttcgc
     8161 tttcaagtcc ctgttcgggc gccactgcta gagattttcc acactgacta aaagggtctg
     8221 agggatctct agttaccaga gtcacacaac agacgggcac acactacttg aagcactcaa
     8281 ggcaagcttt attgaggctt aagcagtggg ttccctagtt agccagagag ctcccaggct
     8341 cagatctggt ctaaccagag agacccagta caggcaaaaa gcagctgctt atatgcagga
     8401 tctgagggct cgccactccc cagtcccgcc caggccacgc ctcccatggt aatagcgatg
     8461 actaatacgt agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa
     8521 tgccaggcgg gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac
     8581 acttgatgta ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg
     8641 aaagtcccta ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg
     8701 tcgttgggcg gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata
     8761 tatgggctat gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa
     8821 tgtcgaccca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     8881 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     8941 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     9001 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     9061 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     9121 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     9181 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     9241 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     9301 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     9361 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     9421 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     9481 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     9541 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     9601 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     9661 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     9721 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     9781 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     9841 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     9901 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     9961 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
    10021 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
    10081 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
    10141 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
    10201 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
//
