LOCUS       sequence-407510-        9954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, FGFR3_TACC3 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_FGFR3_TACC3
FEATURES             Location/Qualifiers
     source          1..9954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(1799..1822)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(join(4715..5425,5426..5428,5429..5431))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4757..4778)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     5106..5125
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5365..5386
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5428..5437
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5513..5716)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5542..5562)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5717..6020
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(6277..6321)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6506..6739
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    7099..7216
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7708..7833
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7880..8137
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        8139..8518
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8549..8653
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8654..8722,8723..9514)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8872..8891)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9685..9954,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcact
     1801 tgtcgtcatc gtccttgtag tcgatttttt ccatcttgct gatcaggtcg tcgcagatcc
     1861 gggtgagttc ttcgttctct ttggtctttt gctcgactgt tttttccagg ctctggatcc
     1921 gcatctgttc tttccgcagg gaagcctgca gtgccagtgc ttcagcctgg gccttggacc
     1981 gcacttgggc aatctcttca ttggccagct ggagtttttc ctcggcgtga gccttcaggg
     2041 cctgataccg ctgcccctct tgtgtgatcc gagccaggta gtcctcgacg cacttcttca
     2101 gggattcctc gttcttccga tagccctcga tcacttcttt ctgtttttcg aagcgcttaa
     2161 acaggtcgct gaagctcttt tccatgctgt tcaggtccgt ggtgagctgg tccttttctt
     2221 tcaggacttt ctggatctcg gccttagaca gctctttctg cttctggacc tcttccatgg
     2281 cctggtacac cacctcttcg aatctgtcca tgatcttgcc gagttccagg ttcttgccgt
     2341 ggagttcctc gcatctgctc cgcagctcgc ggttttcctc ttgggtggcc ttcacgtcgg
     2401 tggaggtcac ggtcagcacc ctgtccaggt cctcaaccag ctgtttgaag gtaggccgct
     2461 ggcttggagc ggcgtgccag cattcccgca tgatcatata caggtcgtgg gtgcaattgg
     2521 cgggcttatc catccggtgg ccctctttca gcagcttgaa cagttcttcc acggggatgc
     2581 cagggtaggg gctgccgccc agtgtgaaga tctcccacag cagcacgccg aaggaccaca
     2641 cgtcagactg gtgggtgtac actctgtcga acagggcctc aggggccatc cacttcactg
     2701 gcagccggcc gttggtggtt ttcttgtagt aatccagatt gtgcacatcc cgggccaggc
     2761 cgaaatcggc gatcttcatg acgttgtcct cggtcacgag cacgtttctg gcggccaggt
     2821 cccggtggat gcacttctgg gaggccaggt attccatgcc tctggccacc tgataggcgc
     2881 aggacacgag gtccttaaag gtcagctgtt cctcgggtgg cttgcaggta tcgaagctgt
     2941 agtccaggcc gggaggcctt ctggctctca ggaactcgcg caggttgcct ttggcggcgt
     3001 attcgaccag cacatacagg ggtccgccct gggtgcaggc tcccagcaga ttgatgatgt
     3061 tcttgtgctt gccgatcatc ttcatcattt ccatctcgga caccaggtcg gacaggtcct
     3121 tgtctgtggc gtcgtccttc agcatcttca ctgccactgt cacgggcttg gcggctctat
     3181 ccttatcgat gccgatggcc tcggccatca caacctgtcc aaagcagccc tcgcccagag
     3241 gcttgcccag ggtcagtcta gctctgctca gctcccactt ggggtcggct ggcagttcca
     3301 gctctgacac gttggccagg gttgggcctt cgccgctaga cagtctggcg atccgcacga
     3361 gaggggtgtt gctgctcatg gaggcgttgc tttccaggga cacctgtctc ttcagaggga
     3421 accggctgat cttgtgcacg gtaggggagc ccaggccctt cttaggtggg cttctcagtc
     3481 tgcacagggt cacggcggcc acaaccagga tgaacaggaa aaagcccacg ccgtagctca
     3541 ggattccggc gtacacgctt ccggcctcgt cggcttccac cagttcctct tcggcgggca
     3601 gcaccaccag ccaggcagaa tggtgggaga agccgatgct gttgccggcc agacatgtgt
     3661 actcgccggc atcctcgaag gtcacgttgt gcagggacag cacttccagt tctttgtcgg
     3721 tggtgttagc gccggctgtt ttcagcacgg tcacgtaagg tgtgccgtcg gggcccactt
     3781 tgctgccgtt cacttccacg tgtttcagcc actggatgtg gggctgggcg tcgctgtaca
     3841 ccttgcagtg aaattccacg tcgctgccca gcacggctgt ctgattggca ggcagtccgg
     3901 cctgcaggat gggtctgtga gggcttcttt ccagcacgtc cagggtgtag gtctgccgga
     3961 tgctgccgaa cttgttttcc accacacagg tgtagttgcc ccggtcggag ggcaccacgc
     4021 tttccatgac cagggaccac tgctggtgtc tcagcttaat gccgccgatc cggtgctcgc
     4081 cccggaactc tcttccgttc ttcagccagc tgatggaagg ggtggggttt ccggcggctg
     4141 ggcatctaaa ccgcacggtg ttggcggcag gcacggccag cagtttcttg tccatccgct
     4201 cgggtctggt ccagtaaggg gcgcctgtgt ccacgccggt atcttcggcc tcatcctcgc
     4261 catcctcgtc gtcgccgctg ctaggggcat cggtcactct gacggagaag tggcacagca
     4321 cccgctgggt cagcctctgt ctgcagctgt aggcgccgga gtcctcgtga gaggcgttca
     4381 ggacctgcag tctctgaggg ccaacgagca ctctctcgct aggcaccagt ccggtgccgt
     4441 ccttaaccca cactgtaggg cccatagggc cgccaccagg aggaggacag ctcagttcca
     4501 cggcgtcgcc agagccaaac accagctgct cttgctggcc aggctcaggt ccaggcactt
     4561 cagcggctct gcccacgact ctctgctctg tgcccagaga ctcgctagag gcgccagcca
     4621 caatagccac ggccacacac agagccaggg cacaagctgg tgctcccatt ccccgggaag
     4681 cggccgcact tcccccggag cctccgcttc cgcccttgta cagctcgtcc atgccgagag
     4741 tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc tcgttggggt
     4801 ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc acggggccgt
     4861 cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg tcctcgatgt
     4921 tgtggcggat cttgaagttc accttgatgc cgttcttctg cttgtcggcc atgatataga
     4981 cgttgtggct gttgtagttg tactccagct tgtgccccag gatgttgccg tcctccttga
     5041 agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac ttcacctcgg
     5101 cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg acgtagcctt
     5161 cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag cggctgaagc
     5221 actgcacgcc gtaggtcagg gtggtcacga gggtgggcca gggcacgggc agcttgccgg
     5281 tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg ccctcgccgg
     5341 acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg ggcaccaccc
     5401 cggtgaacag ctcctcgccc ttgctcacca tggtggcgac cggtggatcc cgggcccgcg
     5461 gtaccgtcga ctgcagaatt cggttcacta aacgagcccg gttcactaaa cgagctctgc
     5521 ttatatagac ctcccaccgt acacgcctac cgcccatttg cgtcaatggg gcggagttgt
     5581 tacgacattt tggaaagtcc cgttgatttt ggtgccaaaa caaactccca ttgacgtcaa
     5641 tggggtggag acttggaaat ccccgtgagt caaaccgcta tccacgccca ttgatgtact
     5701 gccaaaaccg catcaccatg gtaatagcga tgactaatac gtagatgtac tgccaagtag
     5761 gaaagtccca taaggtcatg tactgggcat aatgccaggc gggccattta ccgtcattga
     5821 cgtcaatagg gggcgtactt ggcatatgat acacttgatg tactgccaag tgggcagttt
     5881 accgtaaata ctccacccat tgacgtcaat ggaaagtccc tattggcgtt actatgggaa
     5941 catacgtcat tattgacgtc aatgggcggg ggtcgttggg cggtcagcca ggcgggccat
     6001 ttaccgtaag ttatgtaacg cggaactcca tatatgggct atgaactaat gaccccgtaa
     6061 ttgattacta ttaataacta gcacgcgttc gaagcttgag ctcggtcccc tcggggttgg
     6121 gaggtgggtc tgaaacgata atggtgaata tccctgccta actctattca ctatagaaag
     6181 tacagcaaaa actattctta aacctaccaa gcctcctact atcattatga ataattttat
     6241 ataccacagc caatttgtta tgttaaacca attccacaaa cttgcccatt tatctaattc
     6301 caataattct tgttcattct tttcttgctg gttttgcgat tcttcaatta aggagtgtat
     6361 taagcttgtg taattgttaa tttctctgtc ccactccatc caggtcgtgt gattccaaat
     6421 ctgttccaga gatttattac tccaactagc attccaaggc acagcagtgg tgcaaatgag
     6481 ttttccagag caaccccaaa tccccaggag ctgttgatcc tttaggtatc tttccacagc
     6541 caggattctt gcctggagct gcttgatgcc ccagactgtg agttgcaaca gatgctgttg
     6601 cgcctcaata gccctcagca aattgttctg ctgctgcact ataccagaca ataattgtct
     6661 ggcctgtacc gtcagcgtca ttgacgctgc gcccatagtg cttcctgctg ctcccaagaa
     6721 cccaaggaac aaagctccta ttcccactgc tcttttttct ctctgcacca ctcttctctt
     6781 tgccttggtg ggtgctactc ctaatggttc aatttttact actttatatt tatataattc
     6841 acttctccaa ttgtccctca tatctcctcc ttatggtgtt ttactaaact tttccatgtt
     6901 ctaatcctca tcctgtctac ttgccacaca atcatcacct gccatctgtt ttccataatc
     6961 cctaatgatc tttgcttttc ttcttggcac tacttttatg tcactattat cttgtattac
     7021 tactgcccct tcacctttcc agaggagctt tgctggtcct ttccaaagtg gatttctgct
     7081 gtccctgtaa taaacccgaa aattttgaat ttttgtaatt tgtttttgta attctttagt
     7141 ttgtatgtct gttgctatta tgtctactat tctttcccct gcactgtacc ccccaatccc
     7201 cccttttctt ttaaaattgt ggatgaatac tgccatttgt actgctgtct taagatgttc
     7261 agcctgatct cttacctgtc ctataatttt ctttaattct ttattcatag attctactac
     7321 tccttgactt tggggattgt agggaatttc caggtctgaa gatcctgctg cttgctgtgc
     7381 ttttttctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct tggtgtcttt
     7441 tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata atgatctaag
     7501 ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc tacagccttc
     7561 tgatgtttct aacaggccag tattaactgc gaatcgttct agctccctgc ttgcccatac
     7621 tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt tttcccatcg
     7681 cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct ccttctagcc
     7741 tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc ttgccgtgcg
     7801 cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt tcgctttcaa
     7861 gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg tctgagggat
     7921 ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag
     7981 ctttattgag gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc
     8041 tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag
     8101 ggctcgccac tccccagtcc cgcccaggcc acgcctccca tggtaatagc gatgactaat
     8161 acgtagatgt actgccaagt aggaaagtcc cataaggtca tgtactgggc ataatgccag
     8221 gcgggccatt taccgtcatt gacgtcaata gggggcgtac ttggcatatg atacacttga
     8281 tgtactgcca agtgggcagt ttaccgtaaa tactccaccc attgacgtca atggaaagtc
     8341 cctattggcg ttactatggg aacatacgtc attattgacg tcaatgggcg ggggtcgttg
     8401 ggcggtcagc caggcgggcc atttaccgta agttatgtaa cgcggaactc catatatggg
     8461 ctatgaacta atgaccccgt aattgattac tattaataac tagtcaataa tcaatgtcga
     8521 cccaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     8581 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     8641 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     8701 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     8761 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     8821 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     8881 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     8941 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     9001 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     9061 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     9121 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     9181 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     9241 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     9301 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     9361 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     9421 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     9481 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     9541 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     9601 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9661 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9721 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9781 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     9841 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9901 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgg
//
