LOCUS       sequence-407512-       11388 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, ETV6_ABL1 from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11388)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 11388)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_ETV6_ABL1
FEATURES             Location/Qualifiers
     source          1..11388
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(6149..6859,6860..6862,6863..6865))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6191..6212)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6540..6559
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6799..6820
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      6862..6871
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(6947..7150)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(6976..6996)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7151..7454
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(7711..7755)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    7940..8173
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    8533..8650
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    9142..9267
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             9314..9571
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        9573..9952
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9983..10087
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10088..10156,10157..10948)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10306..10325)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(11119..11388,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac tatctttgaa
     1801 caatgtcgga aatctcctta acgcttgaga gcagcttgct aaaatcttga gttgccgccg
     1861 gaccgctgcc ggcggttgcc gggcagatct gcaactcgcg caggttgttc tccagtttgt
     1921 tgatggcctc acggaatgcg aatttattac gcatctgctg gatgctatcg acgtaagaaa
     1981 cgcagaaggt gtacaggttt ttacctgctt caagaacggc gctatgagat gccatctgct
     2041 ccgagttgcg agagatcgcc aggcaaagtg cttcggtgct atccagaact actcccttgg
     2101 tgatggcacc gctcgcgatg cgctccggcg gctgacgggt tttacgcagg ctaacacggg
     2161 tggatatcag cggaataaac gcagtgctgc tcggctgatc accagcgagt gcggaactcg
     2221 cgctcggcaa agtgctcggc accggggccg ggctgatcgg ggtaccactc ggcttcgcac
     2281 tctgcggttt gggcgtcgca ggcagaaccg gtttcttcag accctccccc ggctgagacg
     2341 gtttggcggc gtcgctgtta accgcatcca ctaggctcgt ggctttggtc ttcgcgccta
     2401 ggactgcttc acccgccgcc tcttggctag gtgactggga cggcttacca ccagccttac
     2461 ccgcgctcgc agccggcggt ggcggcggcg ccggtttcaa tctgctcaat ttgcctttgt
     2521 cgcgccccgg actttcagag ctatgtttgt gacgacgtac cctgctctct tctgcaggac
     2581 cctttgaagt accaccaggg gcgccgctgc cagccttaga ggtaggggtg accggctcag
     2641 ccgccgccgg agtgcctaaa gcagaacctt tacccgcttc ctctttatgc ggaagaccag
     2701 acgccggagc aacggtgact tgacggcgta acggcttagg ggtcaagttc ggcgggcttg
     2761 aacctggcga gctttccatg atgtctttaa aaacttcatc cgccgcctct tcgttcttct
     2821 tgaccaaacg tggcggaggg gtcaccgtac ctctggtaac ctggtccgag cggttttcac
     2881 ccgcgcgctt gcgcggtagc gccggcttct cgctcttatg gccaccgaag gtgctgctat
     2941 caaattggcg accggtggat tgcaggtcgc gaggcagggt caccgaacgc cattccgtgt
     3001 ccttcgcgcc gtgtggaacg cagctcgcgc tgcagctgcg caggaaacgt ttgctgctag
     3061 atccgccacc ttcctcttca ccggtcgcca aacgactaga ggtcaaggtg gagctttttt
     3121 tccacaggtg cggggaacga aaacccgaac cgccactttc tctaagtgcg ccgttcggca
     3181 cacccgcgcc gttggacggc ttagggctct tggccggatc ggcggtgtcc agcggggtga
     3241 acgccagtgc gccgttgctg atgtctcgac cctcttcttc gcccgcacca cgacgctccg
     3301 gttgaccatc catttcgcgg aaagagctgc tacgtttcgg cggcgttgga gccgtcttct
     3361 ttttcttctt gatcagtgcg ctgaacaggt tcgtcttttt atccttcggc aaaagacgtt
     3421 cgtcttcgtt caagccacct tccggcgggc cacgctcctt cctcggcagt aacgggctca
     3481 ccgccggttc atgatccagc gggtcgctct cgccttgacc tttactatgc ggcatctccg
     3541 gaacatccgt ggtgtcacga tgctcagccg ctctacgact ggtacgggtt ttggttggca
     3601 gttcaggcgc ttgcagcagt gtagacaccg caccacgcac gccctgtttt ccgagttctt
     3661 tttcaacctc gtctgaaata gacgactcct gaaacatcgt ttcaaacgcc tgatggattt
     3721 ccgcaaagct cggacgatcg gaagggttcc actgccaaca tgcacgcatc agttcgtaca
     3781 ccttctccgg gcagccctct ggacgttcca tacggtagtc tttttccaat aattcgtaga
     3841 cttgcgacag gtcgatcccc ggatacgggc tcatgccgta ggtcgcgatt tcccataaca
     3901 aaacaccaaa tgcccacacg tcggatttaa tgctaaactt attatacgca agggactctg
     3961 gagcggtcca tttgatcggg aacttcgcgc cggcatgcgc ggtgtaggta tcacccgtca
     4021 taagacgaga caagccgaag tccgcaactt tgactaggtg gttctcacca actaaacaat
     4081 ttcgcgccgc cagatctcga tgaataaaat tcttcttttc gagatattcc atagcggagc
     4141 taatctgagt cgccatgtac agcagaacca ctgcattcac ttcttgacgg ttgcactcac
     4201 gcagataatc caacaggttg ccgtaggtca tgaactcagt gatgatatag aacggcggct
     4261 cacgggtaca aacacccagc agttgtacta aattcgggtg tttgatctct ttcatcaccg
     4321 cggcctcttt caggaactct tccacctcca tcgtgtcctc ttttaaggtt ttgactgcga
     4381 cggtcaggct gtattttttc cagacacctt cataaacctc gccatattgg ccaccgccca
     4441 atttgtgctt catcgtaata tcggttcttt ccatttccca cttgtcatag tttgggctca
     4501 cgccgtaaac ggttggctta ttgcgtttcg gcgccggata gtgcagcgta gtgatcagac
     4561 cgtctgcaac cgttgaatgg tggtgcacca actcggccag ggtgttaaaa cgcgactcgg
     4621 aggacacgta gagcttaccg tcgctcgcgg tattgatgcg gtaatggtaa acgcgacctt
     4681 cgtagcgcag gctaatgctt ctctggcccg ggctggactc gctctcacgc accaggaagc
     4741 tcccattaat gccgctggaa agcagatact cagccgcatt gcggctaacc gggccatgat
     4801 accagctgtg tttctccaaa gagttgaccg gagtgatata atttgacgga acccagccct
     4861 ggccgttctt ggtctgggct tcacaccatt cgccgttgtg gttataaccc agcacacgca
     4921 gcttctctcc cttggtgatg ctcagggtgt tatcgccaga ggctacaaaa tcgtacaggg
     4981 ccacgaacaa gttcggatca ttttcgctcg ggcccgccag caggttctcc ttgctgttcc
     5041 aacgcgccgc ctcgctcagg ccctgcggct cgaagtccga cgccaccgga cgttggagcg
     5101 cctctgcaat acgaccgata ggcattgcgt gttcctcggg tggggagacg ctaaccataa
     5161 tatggttcat gtacgccagg tcctctcggt gggaaaggtt gatcggttta ccctcgcggt
     5221 gcaagccgtc ttcggacaga cggctttgtt tgaaatccac cgagtggcgc gggttcagaa
     5281 tgagcggatg cataatcggg gacggcatga gctgaatcac acgagtactc tcctggcggg
     5341 gagagctcgg cttcggatgc gactcagagc tcgccgggca gtgattgttc tccatcggag
     5401 acaccgacag tgggtatgac tcttggtggt tgttttcttg gtgcggacgc ggaccttgag
     5461 ctctttcggc cggagacaga cggcgaatca tattgtccaa cggtgaacgc agtggacgct
     5521 gctccgggtc cggagacggc cggtgattgg tcgtaatcgg gctacggcta cggtgtagca
     5581 gttcaatggt cggcgggttg tgatgaacat tatccacgga cggacgcggg gtacgctgca
     5641 cgcagttgtc ctcttcgtgg ttttggtgca gaatgacttc cggctgggtg tggatgctat
     5701 tacccgggtg aaaaaacggg ctgaacagga tgcgaggttt acgctgtttc aggatgtgtt
     5761 gcaacagctc gtacaggacg tcaccggagt ggggactacg ataacggaaa tcttctttgg
     5821 ttaacaacag cagcgctttg ccattcattt caaacgtatt ggagtcaatc gggcgcagag
     5881 agaactcgtt ctctgcccac ttcagccact gcgccacatc gtcacgtgac caatagatcg
     5941 gctgcaggcg cagatgcgcc ggcagtcgaa tgctgtcctc ttccatacgc agagctctcg
     6001 gcacaggcac gtgcagcggg gtggaggatg cgtaactcgg cacgggggac tccggcgggg
     6061 tatagctaat gcgctcttgc tttattgaac attgagcggg tgtttcactc atagcggccg
     6121 cacttccccc ggagcctccg cttccgccct tgtacagctc gtccatgccg agagtgatcc
     6181 cggcggcggt cacgaactcc agcaggacca tgtgatcgcg cttctcgttg gggtctttgc
     6241 tcagcttgga ctgggtgctc aggtagtggt tgtcgggcag cagcacgggg ccgtcgccga
     6301 tgggggtgtt ctgctggtag tggtcggcga gctgcacgct gccgtcctcg atgttgtggc
     6361 ggatcttgaa gttcaccttg atgccgttct tctgcttgtc ggccatgata tagacgttgt
     6421 ggctgttgta gttgtactcc agcttgtgcc ccaggatgtt gccgtcctcc ttgaagtcga
     6481 tgcccttcag ctcgatgcgg ttcaccaggg tgtcgccctc gaacttcacc tcggcgcggg
     6541 tcttgtagtt gccgtcgtcc ttgaagaaga tggtgcgctc ctggacgtag ccttcgggca
     6601 tggcggactt gaagaagtcg tgctgcttca tgtggtcggg gtagcggctg aagcactgca
     6661 cgccgtaggt cagggtggtc acgagggtgg gccagggcac gggcagcttg ccggtggtgc
     6721 agatgaactt cagggtcagc ttgccgtagg tggcatcgcc ctcgccctcg ccggacacgc
     6781 tgaacttgtg gccgtttacg tcgccgtcca gctcgaccag gatgggcacc accccggtga
     6841 acagctcctc gcccttgctc accatggtgg cgaccggtgg atcccgggcc cgcggtaccg
     6901 tcgactgcag aattcggttc actaaacgag cccggttcac taaacgagct ctgcttatat
     6961 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
     7021 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
     7081 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
     7141 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     7201 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     7261 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     7321 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     7381 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     7441 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     7501 actattaata actagcacgc gttcgaagct tgagctcggt cccctcgggg ttgggaggtg
     7561 ggtctgaaac gataatggtg aatatccctg cctaactcta ttcactatag aaagtacagc
     7621 aaaaactatt cttaaaccta ccaagcctcc tactatcatt atgaataatt ttatatacca
     7681 cagccaattt gttatgttaa accaattcca caaacttgcc catttatcta attccaataa
     7741 ttcttgttca ttcttttctt gctggttttg cgattcttca attaaggagt gtattaagct
     7801 tgtgtaattg ttaatttctc tgtcccactc catccaggtc gtgtgattcc aaatctgttc
     7861 cagagattta ttactccaac tagcattcca aggcacagca gtggtgcaaa tgagttttcc
     7921 agagcaaccc caaatcccca ggagctgttg atcctttagg tatctttcca cagccaggat
     7981 tcttgcctgg agctgcttga tgccccagac tgtgagttgc aacagatgct gttgcgcctc
     8041 aatagccctc agcaaattgt tctgctgctg cactatacca gacaataatt gtctggcctg
     8101 taccgtcagc gtcattgacg ctgcgcccat agtgcttcct gctgctccca agaacccaag
     8161 gaacaaagct cctattccca ctgctctttt ttctctctgc accactcttc tctttgcctt
     8221 ggtgggtgct actcctaatg gttcaatttt tactacttta tatttatata attcacttct
     8281 ccaattgtcc ctcatatctc ctccttatgg tgttttacta aacttttcca tgttctaatc
     8341 ctcatcctgt ctacttgcca cacaatcatc acctgccatc tgttttccat aatccctaat
     8401 gatctttgct tttcttcttg gcactacttt tatgtcacta ttatcttgta ttactactgc
     8461 cccttcacct ttccagagga gctttgctgg tcctttccaa agtggatttc tgctgtccct
     8521 gtaataaacc cgaaaatttt gaatttttgt aatttgtttt tgtaattctt tagtttgtat
     8581 gtctgttgct attatgtcta ctattctttc ccctgcactg taccccccaa tccccccttt
     8641 tcttttaaaa ttgtggatga atactgccat ttgtactgct gtcttaagat gttcagcctg
     8701 atctcttacc tgtcctataa ttttctttaa ttctttattc atagattcta ctactccttg
     8761 actttgggga ttgtagggaa tttccaggtc tgaagatcct gctgcttgct gtgctttttt
     8821 cttacttttg ttttgctctt cctctatctt gtctaaagct tccttggtgt cttttatctc
     8881 tatcctttga tgcacacaat agagggttgc tactgtatta tataatgatc taagttcttc
     8941 tgatcctgtc tgaagggatg gttgtagctg tcccagtatt tgtctacagc cttctgatgt
     9001 ttctaacagg ccagtattaa ctgcgaatcg ttctagctcc ctgcttgccc atactatatg
     9061 ttttaattta tattttttct ttccccctgg ccttaaccga attttttccc atcgcgatct
     9121 aattctcccc cgcttaatac tgacgctctc gcacccatct ctctccttct agcctccgct
     9181 agtcaaaatt tttggcgtac tcaccagtcg ccgcccctcg cctcttgccg tgcgcgcttc
     9241 agcaagccga gtcctgcgtc gagagagctc ctctggtttc cctttcgctt tcaagtccct
     9301 gttcgggcgc cactgctaga gattttccac actgactaaa agggtctgag ggatctctag
     9361 ttaccagagt cacacaacag acgggcacac actacttgaa gcactcaagg caagctttat
     9421 tgaggcttaa gcagtgggtt ccctagttag ccagagagct cccaggctca gatctggtct
     9481 aaccagagag acccagtaca ggcaaaaagc agctgcttat atgcaggatc tgagggctcg
     9541 ccactcccca gtcccgccca ggccacgcct cccatggtaa tagcgatgac taatacgtag
     9601 atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg ccaggcgggc
     9661 catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac ttgatgtact
     9721 gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa agtccctatt
     9781 ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc gttgggcggt
     9841 cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata tgggctatga
     9901 actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg tcgacccagg
     9961 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
    10021 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
    10081 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
    10141 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
    10201 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
    10261 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
    10321 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
    10381 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
    10441 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
    10501 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
    10561 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
    10621 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
    10681 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
    10741 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
    10801 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
    10861 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
    10921 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
    10981 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
    11041 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
    11101 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
    11161 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
    11221 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
    11281 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
    11341 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgg
//
