LOCUS       sequence-407513-        9618 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, IGH_CRLF2 from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9618)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9618)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_IGH_CRLF2
FEATURES             Location/Qualifiers
     source          1..9618
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4379..5089,5090..5092,5093..5095))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4421..4442)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4770..4789
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5029..5050
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5092..5101
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5177..5380)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5206..5226)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5381..5684
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5941..5985)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6170..6403
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6763..6880
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7372..7497
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7544..7801
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7803..8182
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8213..8317
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8318..8386,8387..9178)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8536..8555)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9349..9618,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcact
     1801 ttactttaaa cagcgtgacg gtagtggagt aaaaaagaga taaaagaaac aggacgatga
     1861 aggttgatgc agtggcccat aagttctcga aaccctcttc gtccgctgag acttccccct
     1921 ccgtggactt gtcaactgta cgctcggtaa cgcggttcgg taaggcttcg tgcgcgacaa
     1981 cgcacgtgta agtttcccca gtattccatt cctcttcaga cactgttaag atactgtgag
     2041 cgaagtaacg acccggtgct tgtggttcag gcataggtgc agaagtcaca tatttttcgg
     2101 gggacaaagg ttgtccgcgt tgcatccatt gaacaaaaac atcagctgga ctaaaccccg
     2161 tgaccaagca cgtgatcgtg gctgactcac gcaggttaag ttgttcacgg gcaggaggca
     2221 gcagataaac atctggacgg tgcagcgcaa cgcccttagg gcgagaaatc gtttgtttca
     2281 gaggactggg taaatcggtg tgtgtaactg tgcaagtgaa gcgctctccg ctattccaat
     2341 catcctcaca gatcgacgct tctcctactg cagaaaaggt agcgtttggg tggctttcag
     2401 agatgtttgt gtgggttttg actgcctctc cattctggcg cgtccaagaa atagtgacgc
     2461 tgtcgtaggt cgtaagatct gtcacaaggc aggtcaactt ggtactctta gtcagaaaga
     2521 tcgaagcaaa gctcggtggg atagcaaaaa cacggatagc ggtgtcctgg tctgggccgc
     2581 acatggatga tgcattttgt tggaaggtca gcccacggtg atccacacga catgtgaaca
     2641 tactctggct taaccaatca ctctccttga tagtcaatgt gctggtaacc ttgtatgttg
     2701 ttgggccgct ttccttggct tcggcctgga cctgatcggt tgtgacgcct gagccgactt
     2761 gctttccctc gcgcaaccag ctgacttgaa tctgacgagg ggagaagccg gttgcctggc
     2821 agattaactt acttttgcgc ggattcccga agaatccgtc gcggggagga acaaatacgg
     2881 atacctttgg tggcagttct gcaattacag gaagcgggac gttcttctct ttgttaccgt
     2941 ttgggtgttg aaccttgcat actacatgct catcggttcc ttgcattaca tccttactag
     3001 gaagcaatac ctggctagtt gctgcatatt tgcctccgcg caatacactt gggaagccac
     3061 gagttgagga gatgtcggaa ttgttcttat acttccaaga gaaggtaatt gaatccggca
     3121 ggaaatcctg agctaagcac ccgaccgcga cggaggaagt atccgagggg ctgttctcgc
     3181 aagaaaccag tggaaacaaa gtcggcgcgc tcgcactccc aagcgcgaca tagctgcgat
     3241 cgttcatgac aaatgtgaat ccaccgatgg taacgacgtc cccgccttgc aggggctggt
     3301 gaggcagttg aagagaccct cctgaagcct ctttctcttc cgtctgtggg tccagcatgc
     3361 gcgggctttc cgcctctgtt ttggctaatt ggacaaccaa cggctcttcc ggccccgatt
     3421 cttgctcggc cccggccatc ttatgtaaat gtgctacgtt ttgtgtatcc gtaatccact
     3481 cctgaaagtt gccttgatga atctcgaaca agccgggaaa gatactcttc gggtcaggga
     3541 cggaagggat caaaaacttc ttgacacgcc acagtttcca taaggataac aacaataaag
     3601 acaccattaa caggattgcc aaggacgaga tcagaatgaa ctttgacagc ttgggcttag
     3661 gcggggtcgg tgtctccgcg catgcgtcac ggatttcgcc acgctgccag caagtgacct
     3721 cgctccagtc ggacgggtac gtatccggac catacacgtc ctccattgcc tttacacgga
     3781 cccagaaaga ataacatttt tcagcgtcta aaccttcaat agttacatta catgtattct
     3841 cttgcttcga ctgccattca gtatcgaaag gagaacggta ttggacttca tacaaaaggt
     3901 ctccgtagct taagtcactg caagttaccg ttacggcatc ttgatgccaa ctaaaacgaa
     3961 catgtttggg ggaagagggc tttaaatagt ataccatcca gcgtgaagct gtgaaaacgg
     4021 gatgggtacc attgcgaata ctgaaataaa ggatatcatc gcgctgttct gcgtctaaaa
     4081 ggcacccaga cgtgtggccc tcctgtaata agtaattcgt acattggtca taggcctcgt
     4141 ccccgttaaa gcgatagtga aacgtcagat tggtacgtga gtacttcgat gcattccagg
     4201 taacctggac cgtttccaga ttgaagtaaa tgatttggat ttgaacgccc tcggcagccc
     4261 caccctgacc cagtgccatc cagccaccta aaaggaaaac agctgcgccc cacagcaaca
     4321 ccaaacgacc cattccccgg gaagcggccg cacttccccc ggagcctccg cttccgccct
     4381 tgtacagctc gtccatgccg agagtgatcc cggcggcggt cacgaactcc agcaggacca
     4441 tgtgatcgcg cttctcgttg gggtctttgc tcagcttgga ctgggtgctc aggtagtggt
     4501 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     4561 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     4621 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     4681 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     4741 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     4801 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     4861 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     4921 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     4981 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     5041 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtgg
     5101 cgaccggtgg atcccgggcc cgcggtaccg tcgactgcag aattcggttc actaaacgag
     5161 cccggttcac taaacgagct ctgcttatat agacctccca ccgtacacgc ctaccgccca
     5221 tttgcgtcaa tggggcggag ttgttacgac attttggaaa gtcccgttga ttttggtgcc
     5281 aaaacaaact cccattgacg tcaatggggt ggagacttgg aaatccccgt gagtcaaacc
     5341 gctatccacg cccattgatg tactgccaaa accgcatcac catggtaata gcgatgacta
     5401 atacgtagat gtactgccaa gtaggaaagt cccataaggt catgtactgg gcataatgcc
     5461 aggcgggcca tttaccgtca ttgacgtcaa tagggggcgt acttggcata tgatacactt
     5521 gatgtactgc caagtgggca gtttaccgta aatactccac ccattgacgt caatggaaag
     5581 tccctattgg cgttactatg ggaacatacg tcattattga cgtcaatggg cgggggtcgt
     5641 tgggcggtca gccaggcggg ccatttaccg taagttatgt aacgcggaac tccatatatg
     5701 ggctatgaac taatgacccc gtaattgatt actattaata actagcacgc gttcgaagct
     5761 tgagctcggt cccctcgggg ttgggaggtg ggtctgaaac gataatggtg aatatccctg
     5821 cctaactcta ttcactatag aaagtacagc aaaaactatt cttaaaccta ccaagcctcc
     5881 tactatcatt atgaataatt ttatatacca cagccaattt gttatgttaa accaattcca
     5941 caaacttgcc catttatcta attccaataa ttcttgttca ttcttttctt gctggttttg
     6001 cgattcttca attaaggagt gtattaagct tgtgtaattg ttaatttctc tgtcccactc
     6061 catccaggtc gtgtgattcc aaatctgttc cagagattta ttactccaac tagcattcca
     6121 aggcacagca gtggtgcaaa tgagttttcc agagcaaccc caaatcccca ggagctgttg
     6181 atcctttagg tatctttcca cagccaggat tcttgcctgg agctgcttga tgccccagac
     6241 tgtgagttgc aacagatgct gttgcgcctc aatagccctc agcaaattgt tctgctgctg
     6301 cactatacca gacaataatt gtctggcctg taccgtcagc gtcattgacg ctgcgcccat
     6361 agtgcttcct gctgctccca agaacccaag gaacaaagct cctattccca ctgctctttt
     6421 ttctctctgc accactcttc tctttgcctt ggtgggtgct actcctaatg gttcaatttt
     6481 tactacttta tatttatata attcacttct ccaattgtcc ctcatatctc ctccttatgg
     6541 tgttttacta aacttttcca tgttctaatc ctcatcctgt ctacttgcca cacaatcatc
     6601 acctgccatc tgttttccat aatccctaat gatctttgct tttcttcttg gcactacttt
     6661 tatgtcacta ttatcttgta ttactactgc cccttcacct ttccagagga gctttgctgg
     6721 tcctttccaa agtggatttc tgctgtccct gtaataaacc cgaaaatttt gaatttttgt
     6781 aatttgtttt tgtaattctt tagtttgtat gtctgttgct attatgtcta ctattctttc
     6841 ccctgcactg taccccccaa tccccccttt tcttttaaaa ttgtggatga atactgccat
     6901 ttgtactgct gtcttaagat gttcagcctg atctcttacc tgtcctataa ttttctttaa
     6961 ttctttattc atagattcta ctactccttg actttgggga ttgtagggaa tttccaggtc
     7021 tgaagatcct gctgcttgct gtgctttttt cttacttttg ttttgctctt cctctatctt
     7081 gtctaaagct tccttggtgt cttttatctc tatcctttga tgcacacaat agagggttgc
     7141 tactgtatta tataatgatc taagttcttc tgatcctgtc tgaagggatg gttgtagctg
     7201 tcccagtatt tgtctacagc cttctgatgt ttctaacagg ccagtattaa ctgcgaatcg
     7261 ttctagctcc ctgcttgccc atactatatg ttttaattta tattttttct ttccccctgg
     7321 ccttaaccga attttttccc atcgcgatct aattctcccc cgcttaatac tgacgctctc
     7381 gcacccatct ctctccttct agcctccgct agtcaaaatt tttggcgtac tcaccagtcg
     7441 ccgcccctcg cctcttgccg tgcgcgcttc agcaagccga gtcctgcgtc gagagagctc
     7501 ctctggtttc cctttcgctt tcaagtccct gttcgggcgc cactgctaga gattttccac
     7561 actgactaaa agggtctgag ggatctctag ttaccagagt cacacaacag acgggcacac
     7621 actacttgaa gcactcaagg caagctttat tgaggcttaa gcagtgggtt ccctagttag
     7681 ccagagagct cccaggctca gatctggtct aaccagagag acccagtaca ggcaaaaagc
     7741 agctgcttat atgcaggatc tgagggctcg ccactcccca gtcccgccca ggccacgcct
     7801 cccatggtaa tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag
     7861 gtcatgtact gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc
     7921 gtacttggca tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc
     7981 acccattgac gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt
     8041 gacgtcaatg ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat
     8101 gtaacgcgga actccatata tgggctatga actaatgacc ccgtaattga ttactattaa
     8161 taactagtca ataatcaatg tcgacccagg tggcactttt cggggaaatg tgcgcggaac
     8221 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
     8281 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
     8341 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
     8401 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
     8461 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
     8521 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
     8581 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
     8641 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
     8701 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
     8761 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
     8821 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
     8881 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
     8941 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
     9001 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
     9061 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
     9121 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
     9181 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
     9241 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
     9301 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     9361 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     9421 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     9481 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     9541 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     9601 taagtcgtgt cttaccgg
//
