LOCUS       sequence-407516-        9261 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, JAK2_TPR from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9261)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9261)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_JAK2_TPR
FEATURES             Location/Qualifiers
     source          1..9261
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4022..4732,4733..4735,4736..4738))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4064..4085)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4413..4432
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4672..4693
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4735..4744
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4820..5023)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4849..4869)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5024..5327
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5584..5628)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5813..6046
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6406..6523
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7015..7140
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7187..7444
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7446..7825
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7856..7960
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7961..8029,8030..8821)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8179..8198)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8992..9261,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagt
     1801 taatattacc tctgttaata ccacctctgc cgcccatggc gtgagatacg cccctttgtc
     1861 ggttaaactg tctacccctg acaccttggc gaagagtagt ttgcagccga actctcctga
     1921 aaggctttgg ctgactagag cttgtgtcta cactgctaga actcggtggg tcctggctag
     1981 tagaagggag atctgactca tcgctagctt gcaccggctc ctcttcctgt tgagagtcga
     2041 tctcaaggcc ggcctcagat gaaataccct cggattcggc ttccacgaaa acctcatctc
     2101 catcgtcgcc tgttgcggtt tcgtttgtag tgctcaaggt gccagtagaa gtcgtcacca
     2161 ttgggacgga ctgacttgcg tgctcgctag catctgaggt agtgctttct gtaaacactg
     2221 tcacaggtgc ggctacctgg aggggcgttg ttgggacgct tcgaccacca ctctcctctt
     2281 catgcgcgag gaaaagggga gtctcgtaca taccgagtcc gccttgagag gcgagttggc
     2341 cgagatcgct atgggaacta ctcgtttgag gcatgtcctc aggaggacca aagcggaatc
     2401 gaggaacacc cgctacttga ggactatgta tagcttctgc aaagccatca gtcctatgag
     2461 gcaccacaag ggtaggcgtg gatggtactg tcctgtcctc atcatcgaaa aaatgttgct
     2521 gcataccacc tatcccaggg gtcaactgga ggcctctgcc aactgactgt cgccgagtca
     2581 tctgtatgcg ttgaaccgga ggacccagct cctgtggagg cgcgtgaatg gtaaggcgcg
     2641 gtgggagagg atgggggggt ctccgtggac tttggggagc cctcggagct tgtctttcgg
     2701 aggctgagct tggctgctgc tccctgctca cttcctgtga aaagctactc tcagctgcgc
     2761 cggtattccc ctccccagaa ttttggctgt ccgcggctct gtgattcccc tctccaccac
     2821 ccatggattc ctcggtctct gtgccagggt cagttccgtc ccccccctca gcgtcatcgg
     2881 cttcgtaccc gtcgttgcca tcggcgctac ccgtcccttc attactatct tcaccttcat
     2941 cccccatgcc agtgtcgtcc tcatcgtcat catcgtcctc ttcatcctcc tcataatctt
     3001 catgttcgcc gtcattttcg tcgtcatcct cttcttcatc atctgaatct attacaatca
     3061 catcatcgcc cttaccttgt ccatcctgag aacttgtagt agtttgctgg tcactttgaa
     3121 gggggccgag atcgatttgc agagattggc tagtctcttc tgaatcctcc ataggcgtgt
     3181 aatctccttg cgtaacaccg aggaagtgca ttgcccacgc caattgcttg gccacttcaa
     3241 gtttccagag tatgttaatg cagtttttgt tcttcttcag ataagtatcc aaggaaccaa
     3301 acttcacaaa ttcttgtacc aaaatattct catccccgca cacacagact ccgtagttga
     3361 gaacaaggtg cttgtgagag agtttgctca tcatagatgc ggcttcaaaa aatgactcag
     3421 aatagtttcg gtgagcttta tccaacactt tgaggagtac ttcagtctcg tgcagttgac
     3481 cataatcccc tacctctcgt ctcacccctt taaatatctt ggtaaatgta ccctgtccaa
     3541 gagattcgtt aaagatcagg tcttcgttcc gtatcttgtg gaaaaccatt tgattcatgt
     3601 gtgtgggccg ctgcagagta ggggatgtcg gaacgtcaga cactccgttt gttctaaata
     3661 ccaacaagtt gctcttgtcc ttcggtttgg gagggcaaca cttggtaaac tgaaaaatta
     3721 tattatcgga cctaactgtc tctatctggt agcaattgag cagatctttc agtgagctaa
     3781 aattcttctt agtgccggag agattatatt cttcattttc attttttgtg atcagacaat
     3841 gtttatactc tattacgttt tctcgctcta ctgcaaatgt gagaaaatac ttattgaagt
     3901 ctttgggtga acacctcagc acataaagac cagtttggtt tccggccttc ttcaactttg
     3961 aaatggcgaa atccattccc cgggaagcgg ccgcacttcc cccggagcct ccgcttccgc
     4021 ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     4081 ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactgggtg ctcaggtagt
     4141 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     4201 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
     4261 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     4321 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     4381 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     4441 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     4501 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg
     4561 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     4621 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     4681 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
     4741 tggcgaccgg tggatcccgg gcccgcggta ccgtcgactg cagaattcgg ttcactaaac
     4801 gagcccggtt cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc
     4861 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
     4921 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
     4981 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
     5041 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
     5101 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
     5161 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     5221 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     5281 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
     5341 atgggctatg aactaatgac cccgtaattg attactatta ataactagca cgcgttcgaa
     5401 gcttgagctc ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
     5461 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
     5521 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
     5581 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
     5641 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
     5701 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
     5761 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
     5821 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
     5881 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
     5941 ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
     6001 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
     6061 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
     6121 ttttactact ttatatttat ataattcact tctccaattg tccctcatat ctcctcctta
     6181 tggtgtttta ctaaactttt ccatgttcta atcctcatcc tgtctacttg ccacacaatc
     6241 atcacctgcc atctgttttc cataatccct aatgatcttt gcttttcttc ttggcactac
     6301 ttttatgtca ctattatctt gtattactac tgccccttca cctttccaga ggagctttgc
     6361 tggtcctttc caaagtggat ttctgctgtc cctgtaataa acccgaaaat tttgaatttt
     6421 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
     6481 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc
     6541 catttgtact gctgtcttaa gatgttcagc ctgatctctt acctgtccta taattttctt
     6601 taattcttta ttcatagatt ctactactcc ttgactttgg ggattgtagg gaatttccag
     6661 gtctgaagat cctgctgctt gctgtgcttt tttcttactt ttgttttgct cttcctctat
     6721 cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt
     6781 tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag
     6841 ctgtcccagt atttgtctac agccttctga tgtttctaac aggccagtat taactgcgaa
     6901 tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc
     6961 tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct
     7021 ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag
     7081 tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag
     7141 ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc
     7201 cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca
     7261 cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt
     7321 tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa
     7381 agcagctgct tatatgcagg atctgagggc tcgccactcc ccagtcccgc ccaggccacg
     7441 cctcccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     7501 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     7561 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     7621 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     7681 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     7741 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     7801 taataactag tcaataatca atgtcgaccc aggtggcact tttcggggaa atgtgcgcgg
     7861 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     7921 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     7981 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8041 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8101 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     8161 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     8221 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     8281 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     8341 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     8401 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     8461 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     8521 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     8581 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     8641 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     8701 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     8761 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     8821 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     8881 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     8941 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     9001 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     9061 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     9121 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     9181 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     9241 cgataagtcg tgtcttaccg g
//
