LOCUS       sequence-407517-       11988 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, KIAA0100_PITPNC1 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11988)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 11988)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_KIAA0100_PITPNC1
FEATURES             Location/Qualifiers
     source          1..11988
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     11..30
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(249..353)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        384..763
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             765..1022
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1069..1194
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1686..1803
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    2163..2396
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2581..2625
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        2882..3185
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3186..3389
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3340..3360
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      3465..3474
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3471..3473,3474..3476,3477..4187)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3516..3537)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3777..3796)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4124..4145
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9389..9408)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9454..9509
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9508..9527)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9870..9886)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9870..9886)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9883..9905)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9894..9910
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9918..9924,9925..9942,9943..9948))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9963..9984
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10043..10238
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10089..10224
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10244..10378
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10281..10300)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10335..10354
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10446..10463)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10617..11205)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10697..10716)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11376..11988,1..179,180..248))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
ORIGIN
        1 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
       61 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
      121 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
      181 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
      241 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
      301 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
      361 ccccgaaaag tgccacctgg gtcgacattg attattgact agttattaat agtaatcaat
      421 tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa
      481 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
      541 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
      601 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
      661 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
      721 tacttggcag tacatctacg tattagtcat cgctattacc atgggaggcg tggcctgggc
      781 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
      841 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
      901 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
      961 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     1021 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     1081 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     1141 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     1201 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     1261 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     1321 actggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     1381 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     1441 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     1501 caaaacaaaa gtaagaaaaa agcacagcaa gcagcaggat cttcagacct ggaaattccc
     1561 tacaatcccc aaagtcaagg agtagtagaa tctatgaata aagaattaaa gaaaattata
     1621 ggacaggtaa gagatcaggc tgaacatctt aagacagcag tacaaatggc agtattcatc
     1681 cacaatttta aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac
     1741 ataatagcaa cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat
     1801 tttcgggttt attacaggga cagcagaaat ccactttgga aaggaccagc aaagctcctc
     1861 tggaaaggtg aaggggcagt agtaatacaa gataatagtg acataaaagt agtgccaaga
     1921 agaaaagcaa agatcattag ggattatgga aaacagatgg caggtgatga ttgtgtggca
     1981 agtagacagg atgaggatta gaacatggaa aagtttagta aaacaccata aggaggagat
     2041 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     2101 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     2161 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     2221 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     2281 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     2341 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     2401 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     2461 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     2521 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     2581 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     2641 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     2701 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     2761 ttatcgtttc agacccacct cccaaccccg aggggaccga gctcaagctt cgaacgcgtg
     2821 ctagttatta atagtaatca attacggggt cattagttca tagcccatat atggagttcc
     2881 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     2941 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     3001 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     3061 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     3121 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     3181 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     3241 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     3301 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     3361 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgggctcgt ttagtgaacc
     3421 gaattctgca gtcgacggta ccgcgggccc gggatccacc ggtcgccacc atggtgagca
     3481 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     3541 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     3601 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     3661 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     3721 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     3781 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     3841 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     3901 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     3961 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     4021 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     4081 cccagtccaa gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     4141 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagggc ggaagcggag
     4201 gctccggggg aagtgcggcc gcttcccgga tgccattgtt cttttccgca ttgctggtct
     4261 tacttcttgt cgcgttatcc gccctgtttt tgggccgctg gcttgtcgtt cgtttggcaa
     4321 caaaatggtg ccaacgtaaa cttcaggcgg aattgaagat tggctcgttc cgcttcttct
     4381 ggattcaaaa tgtttcctta aagttccagc aacatcaaca gacagtggag atcgacaacc
     4441 tgtggatcag cagtaaattg ctgtcccacg atctgcccca ctacgtcgca ttatgtttcg
     4501 gtgaggtacg cattcgcaca gatctgcaga aggtaagtga tttgtccgcc ccattttcac
     4561 agtcggcagg cgttgaccag aaggagctga gttttagccc atccttactg aagatctttt
     4621 gccaactttt cagcattcat gtcgatgcta ttaatattat ggtcctgaag gtagacacaa
     4681 gcgagtcact gtggcacatc caaatctcgc gttcacgctt cctgcttgat tccgatggca
     4741 aacgtttgat ctgtgaggtg tccctgtgta agattaactc taaagttctg aagtcaggac
     4801 agttagaaga tacctgtttg gtggaattgt ccctggcatt agacttatgc ttgaaagtcg
     4861 gtatctcttc tcgtcactta acggctatca cagttgatgt ctggactttg cacgctgaac
     4921 tgcatgaggg attgtttcaa tctcagttgt tatgtcaagg gccgtcactt gcgtcaaaac
     4981 cagttccgtg ctccgaggtc accgagaatc ttgttgagcc gactttaccg ggcctgtttc
     5041 tgttgcagca gttgccggac caagttaaag taaaaatgga aaatacttcc gtcgtcctgt
     5101 ctatgaactc gcaaaagcgt catctgacat ggactctgaa attacttcag tttttgtacc
     5161 accgcgacga agatcagctg cctttgcgtt cattcacggc gaactctgat atggcgcaaa
     5221 tgtcaacgga actgttatta gaagatggat tgttgctttc ccagtctcgc caacgcattg
     5281 tctgcttgaa ctcacttaaa gcatctgttc aagtgacaac cattgatttg tcagcgtctc
     5341 tggtgctgaa cacgtgtatc attcactacc gccatcaaga gttttcccac tggcttcatc
     5401 tgttagcctt ggagactcaa gggagtagta gcccggtatt aaaacagcgc aaaaaacgta
     5461 ccttccccca gattctggcg ccgatcattt tcagtacgag catcagtaat gtcaacatca
     5521 gcatccaatt aggagatact cctcctttcg cattaggctt taattcaatt tcgttggact
     5581 atcaacacct gcgtccacaa tctatccacc aacgtggcgt gttgacggta gaccatttgt
     5641 gttggcgtgt tgggtcggat agccatattc agcgtgctcc tcatccaccg aatatgcatg
     5701 tatgggggga ggctcttgtg cttgacagtt ttacacttca aggcagttac aaccagccac
     5761 tgggtttgtc aagtacccaa tctgacacct tatttttaga ttgtactatc cgcggacttc
     5821 aggtcgaggc ctccgataca tgcgcgcaat gtttgagccg tattttgagc ttaatggggc
     5881 cccaaagcgg taaaagcgca gtttcccgtc acagttcttt tggagaatcc gtttcactgt
     5941 tatggaaagt agatttgaaa gtcgaagaca tgaacctttt caccttgagt gcgctggtag
     6001 gggccagcga ggtgcgcctt gacaccttga cgatcctggg cagtgcggaa accagcacag
     6061 taggcattca gggcttagtc ctggccctgg taaagtcggt cacggagaag atgcaaccgt
     6121 gttgcaaagc accggacatt cccacccccg ttttatcgct gtctatgctg tctatcacat
     6181 atcactcgtc gatccgttca ttagaggtcc agtgcggcgc tggcttgaca ttgttatggt
     6241 ctccccccga ccacatgtac ctgtaccagc acgtgctggc gacacttcag tgccgtgatt
     6301 tactgcgcgc aacggtgttt cctgaaaccg tgccatcatt agcgttagag acaagtggta
     6361 cgacgagtga attggaaggt cgcgctccgg agccattgcc gcccaagcgt ttactgaatc
     6421 tgaccctgga ggtttcgact gcaaaattaa cagcctttgt cgctgaggat aagttcatta
     6481 cattagccgc tgagtcggta tcattatcgc gtcacggagg ctcgctgcag gcttactgcc
     6541 ccgagcttgc agcgggcttt gatggcaact cgatttttaa cttcaaggag gtggaggtgc
     6601 agctgttacc cgagttagag gaaatgatct tgcaccgtaa cccgtttcct gctttgcaga
     6661 cattgcgcaa ccgcgtttgg ttactgtcat tcggcagcgt ctccgtcgaa tttccctacc
     6721 aatacgactt ttcacgtacg cttgacgagg ccgtaggtgt tcagaagtgg cttaagggtc
     6781 tgcatcaggg gacgcgcgcc tgggcctctc cgtcgcccgt accccttccc ccggatctgc
     6841 tgttaaaggt cgagcacttc agttgggttt tcctggatga cgtctttgag gtgaagcttc
     6901 atgataacta tgaactgatg aaggatgaat ctaaggagag tgcgaagcgt ttacaattgt
     6961 tggacgcaaa agtggctgct ctgcgcaagc agcacggcga acttcttccc gcccgcaaga
     7021 tcgaagaatt atatgcttcg ttggaacgta aaaacattga aatttatatc cagcgctctc
     7081 gccgtttata cggcaacact cccatgcgcc gcgccctgct gacatggagt ctggcaggct
     7141 tagaacttgt tgccttggcc gacgcttcat ttcacggccc tgagcacgtc gttgaacagg
     7201 tgcaagagct ggaccccggc tcacctttcc ccccagaggg gctggacctt gtgattcagt
     7261 ggtgtcgtat gctgaagtgc aatgtcaaat catttttggt tcgcatccgc gactaccctc
     7321 gctatttatt tgagatccgt gattggcgtc ttatgggccg cttggtggga acagaacagt
     7381 ctggtcaacc ttgcagtcgt cgccgtcaga tcttacacct ggggttgcca tggggaaacg
     7441 tcgcggttga gcgcaatatg ccaccactta aattctacca tgacttccat agcgagattt
     7501 tccaatacac cgtggtctgg gggccgtgtt gggacccggc atggactctg atcggccaat
     7561 gcgtggacct tcttacaaag ccgtcagctg acccatcacc accccttcct tggtgggaca
     7621 agtcgcgtct tttattccat ggggactggc acatggacat tgagcaagcg aatctgcatc
     7681 aacttgcaac ggaagatccc tacaatacta ccgagaacat gcattgggag tggagtcatt
     7741 tgtcctttca ctggaaacca ggtcagtttg tattcaaagg agaccttgac attaacgttc
     7801 gcactgcgtc aaagtatgat gattgctgct tcctgcatct gccagacctt tgtatgacct
     7861 tagacttgca atggttatgc catggaaacc cacatgatca tcactccgta actttgcgtg
     7921 cgccggaatt tttaccagag gttccgctgg gccaacttca tgacagttat cgtgcttttc
     7981 gctctgagaa tttgaacctt tctattaaga tggatttaac tcgtcattcg gggaccatct
     8041 cacaacctcg cattctttta tacagttcga cattacgttg gatgcagaac ttctgggcga
     8101 catggacctc agtgacacgc cctatctgcc gcggcaaact ttttaacaac ttaaaaccat
     8161 ccaaaaaaaa gctcggacag cactataaac agttgagtta taccgccctt tttcctcagt
     8221 tgcaggttca ttactgggct tcctttgcac agcaacgcgg gattcaaatt gaatgtagtc
     8281 aaggtcatgt gtttactcgt ggtacacaac gcctgattcc gcaagcaggg accgtaatgc
     8341 gtcgcttgat ctcggactgg tctgtaacgc agatggtctc tgacctgagc caggtaacgg
     8401 tgcacctgat ggccagccct accgaggaga atgctgatca ttgtctggac ccacttgtca
     8461 ccaagactca tttgttgagt ttatcatctt tgacttatca acgccattca aaccgcaccg
     8521 cggaagagga attgtcggcc cgcgacggag atcctacgtt tcatacccat caacttcact
     8581 tagtagacct gcgcattagt tggacgacaa caaatcgcga catcgcattt ggtttgtatg
     8641 atggatacaa gaaagcggcc gtgttgaaac gtaatctgtc tactgaagct cttaaaggat
     8701 tgaagattga tccccaaatg ccagcaaaaa agcccaagcg tggcgtacct actagtgctt
     8761 ccgccccgcc tcgtgtaaac actccctcat tctcgggcca gcctgacaag ggaagctctg
     8821 gggacatgac catggatgag gttcgcgagt tcgaacgcgc cactcaggag gcgaccaata
     8881 aaaagatcgg catcttccca cccgccatct ccatcagttc tatcccgttg cttcctagtt
     8941 cggtacgcag cgccccatcc tctgccccga gcacaccatt atcaacggat gcaccagaat
     9001 ttcttagcgt acccaaagac cgtcctcgta agaagtctgc cccagaaacg ttgactcttc
     9061 ccgaccctga aaaaaaagcg accttaaatc tgccgggtat gcattcgtcc gataaaccgt
     9121 gccgccctaa gtcagaatga accggttcta gataagatta cggtaccttt aagaccaatg
     9181 acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg
     9241 ctaattcact cccaaagaag acaagatctg ctttttgcct gtactgggtc tctctggtta
     9301 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     9361 taaagcttca gctgctcgag ctagcagatc tttttccctc tgccaaaaat tatggggaca
     9421 tcatgaagcc ccttgagcat ctgacttctg gctaataaag gaaatttatt ttcattgcaa
     9481 tagtgtgttg gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt
     9541 taaaacatca gaatgagtat ttggtttaga gtttggcaac atatgcccat atgctggctg
     9601 ccatgaacaa aggttggcta taaagaggtc atcagtatat gaaacagccc cctgctgtcc
     9661 attccttatt ccatagaaaa gccttgactt gaggttagat tttttttata ttttgttttg
     9721 tgttattttt ttctttaaca tccctaaaat tttccttaca tgttttacta gccagatttt
     9781 tcctcctctc ctgactactc ccagtcatag ctgtccctct tctcttatgg agatccctcg
     9841 acctgcagcc caagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
     9901 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
     9961 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
    10021 aacctgtcgt gccagcggat ccgcatctca attagtcagc aaccatagtc ccgcccctaa
    10081 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
    10141 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
    10201 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctaacttgt ttattgcagc
    10261 ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc
    10321 actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg tctggatccg
    10381 ctgcattaat gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc
    10441 gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct
    10501 cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg
    10561 tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc
    10621 cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga
    10681 aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct
    10741 cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg
    10801 gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag
    10861 ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat
    10921 cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac
    10981 aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac
    11041 tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc
    11101 ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt
    11161 tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc
    11221 ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg
    11281 agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca
    11341 atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca
    11401 cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag
    11461 ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac
    11521 ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc
    11581 agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct
    11641 agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc
    11701 gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg
    11761 cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc
    11821 gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat
    11881 tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag
    11941 tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcg
//
