LOCUS       sequence-407519-       16653 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, KMT2A_AFDN from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16653)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 16653)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_KMT2A_AFDN
FEATURES             Location/Qualifiers
     source          1..16653
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(11414..12124,12125..12127,12128..12130))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(11456..11477)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     11805..11824
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     12064..12085
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      12127..12136
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(12212..12415)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(12241..12261)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        12416..12719
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(12976..13020)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    13205..13438
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    13798..13915
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    14407..14532
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             14579..14836
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        14838..15217
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        15248..15352
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(15353..15421,15422..16213)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(15571..15590)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(16384..16653,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac tacttggtgt
     1801 tcagttcgtt ctccagttcc gttagcttac ggctggcctt aactttgtta ctcacgtctt
     1861 gaccctgaga gaagaggcgc tggcgttctt tgaacgtcag gttttccggc gcgccgctag
     1921 agcccgggct atcagcatcc tggctttttg aacgtttttc ctttgcgtca cggcaggcat
     1981 catgtgcacc cacagctgcg cccgtgctac ccggatagct gttagggcca gccaagctac
     2041 agtcctcctc ctcttcctct tcgttatacg cgacgaattt ggcggtgaag agggaatccg
     2101 gcgacagaac ctgggttttc aggtagcttg cattgcgctg cggcggcggc ggtggcgcac
     2161 cgggtgccgg gctcgggctt ggcggttcgt agtcacgcgg cagaggcgga cggcataaac
     2221 ccggtgcttc cggctccaac aaacggcggg ccgcctcatc gtgctgcctg cgacgttccg
     2281 cttccaggcg actataataa ccctcttctt gacgccgttt ttcctccgcg tcgcgcttgg
     2341 tacgctcctc ctcctgccgc ctacgctctt cttcttgacg cgcgcggtcc tccgcttcgc
     2401 gtttacgcat ttcttccaat tgttgttgcc tgcgcctttc ctcatcttgg agacgtgccc
     2461 gacgctctgc ctccagacgc tgcataatca gcatggtatc cacatcatca tcctcctctt
     2521 cttcgtcatc ttcgtccttt tgcttgctct cctgtaagcg tttctggaac tgccattcaa
     2581 gcatcagctt acgcagacgg tctgactcct ccgcgctacg atccggtttg ctttgcaact
     2641 cctggatctc ctttgacagc atatcaacga tgtgcatttg ttgctgcttc tccagtttct
     2701 ccttcgcatc gcgtttccac gggtccgggg acaggctatc gccagaggac agttcctcct
     2761 tggacacggt aatatattgc agatcacgac gttcgatggt gcgcggttgt tgctgcggct
     2821 gcagctcgcg gatcacggtt tcctgcggtc gttgcaacgt ggacggcttt tctggtttca
     2881 tctgctgcgc agtcaacggg gaaaacggag ccgggttcag gctctgggta cgcatctggc
     2941 ccagtttgcg ctcttgttcg cggcgcttgc gctcacgctc ttcttcaagc cgcgccttct
     3001 ctttttcata ccaacgctgg tgctcctcac gctttctccg ctctgccgcg gcaacctggg
     3061 cgctaggcag accaatttgg ttcgcgctcg gcggcggcgg caggggcaaa tccatgctca
     3121 taccatcgaa gtcacctgca taatgtaccg gcggcggtgg cggcggcggc ggcagatcgg
     3181 tacgaatcgg ctgggaaaca gcaaccgggg tcgctggaat cgcagccggg gttttccatc
     3241 ggcccgggcc ggacttggtc agggtggagg caggagtgac gcttttgctg ctgtggttca
     3301 ggtgctcttg gctgctcgtg gaggagtcca gggaactcga ctgatttatc cacatatcgc
     3361 tgtcgctctt acgatccata gcagcctcga tacgatgacg ctccagctgg tacgctttgt
     3421 cctcgcgtaa ttcctcctgc gaacgggtca cacgttgaat ggcaatagag ctgtgattgc
     3481 tgtcggtatg catgtgcggt ttttcttcat agttcggcca ctgattctgc ggcggcgcca
     3541 tacgatcttg gctcttggag gccggaaagg tgaaatattc acgggtatag gtttgcgtcg
     3601 ggatcgggta tgcctccgga cgtggtggcg gggtctgttc ttcggtacac aggtttccgg
     3661 tgctgacgct ggtgattttg gctgtggtgc cgctcgcgta cgcggactta ccacccggag
     3721 acgggggttg attcgccacg tttggagaag aacggtgatc cgctctgttc ttcatcagac
     3781 ggtcatcccc cggcagcttc ttcggctcgc tgtactctgc ccaaggcagc tgcggggatt
     3841 ccgggctacc gttctgggta gagttattat agagctcaaa gccctcgctc ttcggacgcg
     3901 gcttaccgga accgcggcga tcgctgatac gctgcatcat gggagagggc tggttcagaa
     3961 gggtcgccag gccgtggtag atggcgcctt gcttcgcaac ttccagagta accacggagc
     4021 tagtacgggt catcagctcg gccgcacgct cttgggacag accgaccagg ctacgaccgt
     4081 caacagacaa gagctggtca cccgccgcca aacggccatc cacatccgcg gcgcctccct
     4141 tcaccacaga cttaacataa atacccagtt tgtcctgacc tgcaccttta gccgccacga
     4201 tcgacagacc cataccgttc tgctttttca gtgttacagt aatgatctct ggctccttgc
     4261 gcagcggttg cgccaactcg gtattttcac gcaacaagtg gctctcgtaa tctgcacctt
     4321 cgaagtagat ggtccaggtg cccggagaac gggtatgcgg gataaggcgg cagaagcctc
     4381 tctggcaaag agggtccagg aactcttgga gaccattcgg aatgttgcgc acaacgtcgc
     4441 aagagtagcc gtcttccggc agcaggaacg gaagttgcag gtccgggtct tcctccagtt
     4501 gcacttctct gccgtcgcta cgcgccagct catccgcggt gttttccgct acggtaacaa
     4561 cattctcgat caaatcagtc gggatgaacg gctcgtccgg tgcgcagtgg tagttttgca
     4621 acagagcttg tagctgcagg ctgttcagtt taaaacaggt gctgttaatg ttcggaatat
     4681 catccggagc atatttatcc atggtaagca gcgtggttgc ctgcacaata cgactaagat
     4741 ggcagtccgc agccagttca agaccctgct tctctgccca tgcttcaata tgacccagtt
     4801 gctgacgaat aatcgcaccc cagtagtggc tgcacagacc agagtccgga tcagtaacca
     4861 agcggttgaa caaccacatg ttgatgaagt gaaacagttg gctgaaaagt tggatcgtca
     4921 acgcggcgtt aacacggcag cgccgcagta agctcatagc accggtgagg gtatgcagta
     4981 cgtcatcaat tttgggacgt tgaaggctgt tctcctctgg gtcgtctagg aaggccggca
     5041 tgtaattgtt cagctcgctt tgcagacagt gaaccaggta tttaaatgcc atctgcacca
     5101 ggtgcgccag aacatcctgc gcgtccaggg tgatacgaga gagatcacga tcttgtttga
     5161 taaagttgag cagttcgctt gcgtttgcca tccagaaagc cagcgcgccc gcgatatttt
     5221 tctgcttttg aataacgcct tccatcatgc tgaccatttt attcacaacc gcaatgactt
     5281 tatgtgttct ctcggtcggg ctgatgtctg gacgatactg gttgctcaga acatagcggc
     5341 acgccatata cagcacgtac gtcggcgaca atttaaagtg aacggttgag ctgttggtgt
     5401 aattgatgat cgcggacaga aagctatcct cgctgctttc acggaactca atagacgccg
     5461 gcaggatgag ttccggaccg gaggcgtcct gcgtcctgga ttcttggcgt cggtagtccg
     5521 gctgttgttc aacacgaatc atcggtttaa ccataccacg ttccgccgta gacgaagcag
     5581 aggaaacacg gtcagagtct aatcgagtgg tgcttttgct ggtcggcagc gctgtaccgc
     5641 tgtggatgtc gccacccaga tcaaaggtgg tttcctgtac aatccccggt ttgtgacgcg
     5701 ggcctttaac catcagccca ccgtccacac tacgcttggc cagagcatgg tcttgcgacg
     5761 ggtccacaaa cttaaaaaca tggctcgcgc caaactgaac tttcatgccg ctttgcaaca
     5821 tcgttgtctc tgaaatacgc tggccttcca cataggtctc cgcgtccatt gaacgcggcg
     5881 tcacggtcac cacgccgtcc atattggtta aatcacaatg atgtggctgg atacctgggc
     5941 cgaacagctg gatagagttg tcgtctagct tctcggtacc tacttcagtg acgctgagtt
     6001 gtagtctgta cagcttcggt ttgtcacggg agtcggaacc gtcttcgtac gtgtggtaat
     6061 tgtaatacgc gaagtgattg cggcgacccg ggctcagctc caccaaatac ggtaattttt
     6121 caggaggcag ggtgctacca taaccagagc catctgcgcg ttctttacct ttcggggtct
     6181 tgccctccag atgttttttg gttttcttcg gaatgtggtc gggcggtcgg cgcttcagct
     6241 gaaacaccag gatgcccttg tcagacggcc actcacgaaa gatttgcaac gggcactcat
     6301 catcgtccag gataatctct ttcgcaccct tttcgtccga atgttgagcg cccggcggca
     6361 acatcacgcg cgcgatgcag taatccttcg gattctcttt ctccaagccg tacttctcca
     6421 gggcttctgc gacggcaaaa tcggctgggt ctgtagttga taatagtatc gttttatagg
     6481 ggatgttcgg tttcaggcta tctgcataga ttcgaagggt accgccacta tcgggacggc
     6541 cgtcactgct gcggaactct tgcatacgct tttccagttt ttgttgccga cgacgtttca
     6601 tgaccacctc cgggttgctg atggtacggg taaagctggt ctccggcata tctttgtaca
     6661 cttccgccgc cagccgactg ttttcgacgt cctcaccctg aaatggacgg tcgtccttgt
     6721 cggacgcctg cctaagcgct tctttttcgc gcttcttctt ttcctttttt tcctttttgc
     6781 tcaaggtacg cttgaaattc tgaataacgc cttccttctc ttgcttctca gggccgttgg
     6841 actgcgcttt tttcggcgga atggcatcgt tctcgttctt cagaacaaaa cgaccctcgc
     6901 gatcgtcctt attccaattc agctggacaa ccaacggttt ctcgtcgatg tccaaccttc
     6961 tttcaccgct aacatgtacc tcgtacaggg agtacttcgg actactcagc attcgcatat
     7021 ccgggcgaaa tttttccgcg agggtctcga tgacatcttg cgtcgtggcg gtagaggaga
     7081 ctctaatgca cttggtcgcg aagttacccg ctgctttatc ctggaaatag aaacgcatca
     7141 cgccgtgaaa ttccaggtcc ttaaaatcca cacggatacg gtgcacacca tccgccggga
     7201 tcttttgttt cgagctattg ccgttggaca gagtagacag gatattaagc gtgcctgcgt
     7261 tctcctgttt attaaccggc ggcggttttt ctttctcttt cggcttttgc ttcaccggaa
     7321 tgctagggcg aggcgccacc tttttttgct tggactgttc cgggccagac tccggaggcg
     7381 gcggttgttt tttcttcggc tcgctcgggg tcgtcttcgg cacctctttt ctgggaggac
     7441 cagtggtcgg cggctgcggc ggaataacca aggctggctg gctaacttgc ttgctggatt
     7501 tcctgctagc gggggtggta gcctgcttgc tttccggacc cggtgctgac acattaccct
     7561 cttctgattt ctcctcgacg ggtttacgtg gcggcggttc tgacgagctt tttttcgggg
     7621 ccggatcttc acgtgcagac ggggtcggtt tctgactgct atcaacaacg ttcttaacaa
     7681 cagagctttc cttggagtcc tttttctcgg aagttttgct cttcttctct ttctttttaa
     7741 ccgccttcgc ttgcttttgc agatacgcct ttgacggcat ccattgcagg ttctggcact
     7801 tacgcatttt gcagcactgc tttttgatgt tacggccacc gaacttcggt ttgtccaggc
     7861 agttggtgca cacgccacag tcctcgggaa cctgacaacc cggacattga ccacaacgac
     7921 gagaacgacg acccttttta accggaggct cctgaggggc tttgttacgg gtgaccggct
     7981 tgatcggttt aatcggcggt gccagcggtt ccgcgtcctc gctacccgca attgatgact
     8041 tgtcgtcgtt tcccatactg ctcaagatct tttctctttc ttcccacggc aacgcggata
     8101 gggtaggcat gtcatccggg aacactgcac gcttacgacc caaggccacc gctgcgcgac
     8161 ggcaaacgtg cttgatgcgc ggaccgcgaa cactcgtttc cgacgagtcg ctctcctggc
     8221 cctgcgcctt cggctggtcg gtctgcttca gactcttgct cttctcgatt ttgcacagtt
     8281 gcgccttcgc cttcttcagc aggcttgcca cgcgcttgtc ggtcattggc agtttgtccg
     8341 cctgcgccag cattgaacca atcgagctag tactgtgctt aacggtagac gagctcgggg
     8401 tgctcaggca taaggtcttc tctttctcca ggctaggcgc cgtcggaccc agatccaggt
     8461 tcgttttttc caggttgcca cgaccttttt taatcagaat cttcgtcttc actgcggtgg
     8521 tatcccccaa cgtaacggag gtgatatccg taccgctgtc gtgggaagaa gacttcttcc
     8581 gaccggtagc ttttttagca gagctagatg tggcaacgtc ttcaccaacc actttctctt
     8641 tcgaaacacg acctaccggg tacagcgccg agctgctctg aatttcgcta cccttttttc
     8701 gtttctcctt acggctctct cgcttatttt ctttttcgcg ttcacgatca cgctcacgag
     8761 atttgtcttt ctccacactc ttatcagcat cacggtcctt gctcaattcc tccggggcct
     8821 tgtctttgtt acgaccacgt tccgtttggg agcccggcgt aaaccatgga aacagcggcg
     8881 tcggggagct ggaagagaac ggttcagctg gcgcggaggt ctgctttctc ggacgctggt
     8941 ttttttcggc gctctcgccg gactgggtca aagagtggga cgggaaggtg aaggtcggat
     9001 tcagcgcgga cgtagccaaa ggagagacag aaatgctcag agaggaggac acggaagaag
     9061 gcggggtcag cgggcttaac tcggaggaag acagacggcc gctgcgggta cgcatggagt
     9121 ggctcggaga acgcggttcg cttctaatcg ggctaaaaac cttacgttta cgcttgcgat
     9181 tcgacacgcc gctgcttgag gtacccgccg aggtacgatt gctcggaagg gtgaccgatt
     9241 caaagatacg agagtgcgcc tcgctcggtg tgaaccgtgg cgcgcgcaac agggggctcc
     9301 gtttatgcat gtcgaagcgg gtgccgctat gcagtgggct aaacaaacgc gctgaagcgg
     9361 cggtgccgga tgccgagaag ccagatgcaa aaccgacatc ttctggagtt aacggcggcg
     9421 gacggaaatt atcaaaaatc ggtttacgta tcagaccttc ttttgcgtat ttcgctgagc
     9481 taaaatattg cggctcgctc ctggagtgtt tcaatgaggt ccaacggaaa gtcggttcac
     9541 gcaggatcga tttacgtttt ccctgcatcg gtgcggtaga cgctggcagg aacggggatg
     9601 ccaacgggat ggtcggcggc atcagccacg gggtatggtc gctgatggaa ctcgccggtt
     9661 gaagcggagg cggcggggtc agcagcgggg gcggtggcga gctggaggtt tgctgctgcg
     9721 gagcagattg cagggtagac agttttttgg ttgttctgct accaaagcta cgctcgctta
     9781 cgctgtagcg acggctccgg cggtcattgc tctcgttctc tgggctttgg gaaatcggca
     9841 gcggcggatg aacctccggc gtatcactgc gttcctccgg tagcacttga atctcctcag
     9901 acgcctggga atctgtgctg gtatccactg acggggagga gctgcgcgag ctatcgctgc
     9961 tcatttggga ggaatgctgg gacgcggcgc tgcttttctc ggacgaaccg cagctgggcg
    10021 cgctgaaacg actgttcggc gtgctttcca ggcgtgcgat tttaatcggc ggatcataat
    10081 cttcatcttc gatgaagcgt ctcggagtct taatgatacg gctggaaatc gcgcttacaa
    10141 ccggcatgat aaactgacga atgtttttaa cctgcgtttt caccttacgg ccttggagtt
    10201 gcgcagcctc cttctcgatc tttttttgtg cacccttttt ggcacgctgc agcagctgtt
    10261 tggcgatggt tgcgtccgtg cgttttgatg agggaatgat acgcaccggt ttgatgcggc
    10321 gcgggctctg acgcacaacg gtcttgtcct ctttggtcag cggcggagta ccttctttgt
    10381 ccttacgaac tttttgcggc ttttccagct cgctgttgat caacaagcca gatggagttt
    10441 taatacgttc ggtgctcggc ggacggccac gacgtcttac gatctgcaca cccttacgac
    10501 cgatttggag tttaccggtt ttgaatttgg atttcagcgg cgacagttta ccagcccgta
    10561 attttttaat cttcgtagcc tgttgaaacg tagcactcgg ggtacgttta attttcttca
    10621 agctatcctc cttgttgcct ttcggcagct cagagatatc cttaccatgg gtgattttga
    10681 tttttacgcc cgggaatgtt ggcgggcggc cacgcttctt ctcgatagac ttgctgtcct
    10741 tcttcttgat tttatcccca gatttagttt cgctcttatt aagcgggctg aacacggacg
    10801 ggtcgcttag aattgcactg ttgcggtcgg acccgctacg cgggcggcca cgcggcttgc
    10861 gcgggctggt cttcacgctg ggagaacggg tcggagaacg aactcgaact tcctcgtcag
    10921 aaccaaaacc cagaaactgt tcatcttcgc cgctaccacc cccgccgcct gattcgccga
    10981 aaacagctcg gaaacgacgc aggttcgtgc cgatagccgc gctcacctgc agagccgcat
    11041 cgaatcccgg acccacacgc agcagtgccg gaccgcttga cgcggagcta gagctagagg
    11101 agctgctgga ggcgctgctg gatgacgcgg cggacgccgc cgctgcgccc cccggaacgc
    11161 ctgcgccgga tgaaccagca gccgccgccg ccgccgcaac ggcggggggg ctcggtggcg
    11221 cacccggacc accgccaccg acaggtggac caggcggcaa caaaagcgcc ggcacacgct
    11281 gacgtggcgc gccgcccaga ccgcggcgac caccaccgcc gccaccaccg gtcgtgcccg
    11341 gacgggcggg aaacctccat ctacatgagt gagccatagc ggccgcactt cccccggagc
    11401 ctccgcttcc gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
    11461 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
    11521 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
    11581 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
    11641 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
    11701 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
    11761 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
    11821 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
    11881 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
    11941 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
    12001 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
    12061 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
    12121 tgctcaccat ggtggcgacc ggtggatccc gggcccgcgg taccgtcgac tgcagaattc
    12181 ggttcactaa acgagcccgg ttcactaaac gagctctgct tatatagacc tcccaccgta
    12241 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
    12301 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
    12361 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
    12421 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
    12481 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
    12541 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
    12601 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
    12661 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
    12721 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
    12781 cacgcgttcg aagcttgagc tcggtcccct cggggttggg aggtgggtct gaaacgataa
    12841 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
    12901 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
    12961 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
    13021 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
    13081 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
    13141 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
    13201 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
    13261 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
    13321 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
    13381 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
    13441 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
    13501 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
    13561 atctcctcct tatggtgttt tactaaactt ttccatgttc taatcctcat cctgtctact
    13621 tgccacacaa tcatcacctg ccatctgttt tccataatcc ctaatgatct ttgcttttct
    13681 tcttggcact acttttatgt cactattatc ttgtattact actgcccctt cacctttcca
    13741 gaggagcttt gctggtcctt tccaaagtgg atttctgctg tccctgtaat aaacccgaaa
    13801 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
    13861 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
    13921 gatgaatact gccatttgta ctgctgtctt aagatgttca gcctgatctc ttacctgtcc
    13981 tataattttc tttaattctt tattcataga ttctactact ccttgacttt ggggattgta
    14041 gggaatttcc aggtctgaag atcctgctgc ttgctgtgct tttttcttac ttttgttttg
    14101 ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac
    14161 acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag
    14221 ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagt
    14281 attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt
    14341 tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt
    14401 aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg
    14461 cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct
    14521 gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg
    14581 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
    14641 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
    14701 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
    14761 gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc
    14821 gcccaggcca cgcctcccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
    14881 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
    14941 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
    15001 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
    15061 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
    15121 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
    15181 attgattact attaataact agtcaataat caatgtcgac ccaggtggca cttttcgggg
    15241 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    15301 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    15361 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    15421 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    15481 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    15541 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    15601 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    15661 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    15721 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    15781 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    15841 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    15901 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    15961 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    16021 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    16081 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    16141 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    16201 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    16261 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    16321 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    16381 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    16441 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    16501 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    16561 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    16621 tgctgccagt ggcgataagt cgtgtcttac cgg
//
