LOCUS       sequence-407521-        9024 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, ETV6_NTRK3 from patient-derived
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9024)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9024)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_ETV6_NTRK3
FEATURES             Location/Qualifiers
     source          1..9024
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3785..4495,4496..4498,4499..4501))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3827..3848)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4176..4195
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4435..4456
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4498..4507
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4583..4786)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4612..4632)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4787..5090
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5347..5391)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5576..5809
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6169..6286
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6778..6903
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6950..7207
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7209..7588
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7619..7723
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7724..7792,7793..8584)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7942..7961)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8755..9024,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcagc
     1801 ccagaatatc caggtaaatc ggcgtagcct tcccaagagc gtgaagaatt ttatagattt
     1861 ccttaatatt cagacgctgt tgcggctcac gctgccagca ccccagcatt acgtcataaa
     1921 cttcttttgg gcacacgcgt gggcgttcaa gaacacgccc ttgggtaatg cattcgatca
     1981 cctcggtgtt tgacagctga aaccaaggtt gctttccata cgtaaagatt tcccacagga
     2041 taacaccaaa gctccaaacg tcgctctccg tggtgaattt gcggtacatg atggattccg
     2101 ggggcatcca gcggataggt aacattgtgt gaccacctac ctcgcaccaa atgcaaaaat
     2161 cgttccctga agggttgaac agacggtaat agtctgttga gtaaacgtca cgactcatac
     2221 cgaagtcccc gatcttaact aacaggtttg caccaacaag acagttgcga gtagcaagat
     2281 cacgatgcac gaagtgctga gaggcaaggt ataccattcc cgacgcaatc tgcgacgcga
     2341 tgtgaagcat ttgggacagg cccagttcac ctttagcctg acgtggttga ccgtccacaa
     2401 gaatcatcgc gtctggaccg tgcgcacgca ggaatttatt caggtctcca tgcttcatgt
     2461 attcgaagac catgatcaac ggatcaccat ctccacaaac cccgtagaat ttcacaatgt
     2521 gttcatgctg caagttggtt aataattccg cttcacgttg gaagtcttta cgggccgcca
     2581 gggttggatc tttaagcgct tttacggcta ccaacatttt gtcttttgtc ggacttaaat
     2641 tgtagcactc tgccagaaac accttcccaa atgcgccttc ccctaattcg cgtttcagta
     2701 cgatgtcacg acgcttaata tgttgcacat ctgcgatgcg accgattggc atggcatgtt
     2761 cctcgggggg gcttaccgaa accatgatat ggttcatata agcaaggtcc tcacgatgtg
     2821 acaagttaat tggctttcct tcacggtgaa gtccatcctc tgacaagcga ctttgcttga
     2881 agtctacgga atggcgcggg ttaaggatca atgggtgcat aataggagac ggcataagtt
     2941 ggattacacg cgtactttct tggcgtggac tgcttggctt ggggtggctc tctgacgagg
     3001 cagggcaatg gttgttttcc atgggagaaa cgctcaacgg ataactttct tggtgattgt
     3061 tttcttgatg cggacggggg ccttgcgcgc gttcggccgg gctcagacga cggatcatgt
     3121 tgtctaaggg actacgcagt ggacgttgct ccgggtcggg tgaaggacgg tggtttgtcg
     3181 tgattgggct acggctacgg tgaagcagtt cgatggtagg tggattgtga tgtacgttgt
     3241 caacggaagg gcgtggggtg cgttgtacgc aattgtcctc ttcgtgattc tggtgtaaaa
     3301 ttacctctgg ttgtgtgtgg atggagttac cgggatgaaa gaatggagaa aacagaatgc
     3361 ggggcttacg ttgtttcaaa atatgctgca gcagctcata taagacatct ccactatgcg
     3421 gactacgata acgaaagtct tctttagtta acaataacaa tgcttttccg ttcatctcaa
     3481 aggtgttaga gtcgatgggg cgcaaggaaa attcgttctc cgcccatttc agccattgtg
     3541 cgacatcgtc gcgagaccag taaatcggtt gtaaacgcaa gtgcgcgggc agacgaatgg
     3601 aatcttcttc catgcgcaat gcgcgcggaa ccggaacgtg taagggggtc gaggacgcat
     3661 aagaagggac aggactttct ggaggcgtat aagaaatgcg ttcttgctta atggaacact
     3721 gtgcaggagt ctcggacatt ccccgggaag cggccgcact tcccccggag cctccgcttc
     3781 cgcccttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     3841 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag cttggactgg gtgctcaggt
     3901 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     3961 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
     4021 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
     4081 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
     4141 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
     4201 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
     4261 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
     4321 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
     4381 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
     4441 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
     4501 tggtggcgac cggtggatcc cgggcccgcg gtaccgtcga ctgcagaatt cggttcacta
     4561 aacgagcccg gttcactaaa cgagctctgc ttatatagac ctcccaccgt acacgcctac
     4621 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     4681 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     4741 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     4801 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     4861 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     4921 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     4981 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     5041 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     5101 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gcacgcgttc
     5161 gaagcttgag ctcggtcccc tcggggttgg gaggtgggtc tgaaacgata atggtgaata
     5221 tccctgccta actctattca ctatagaaag tacagcaaaa actattctta aacctaccaa
     5281 gcctcctact atcattatga ataattttat ataccacagc caatttgtta tgttaaacca
     5341 attccacaaa cttgcccatt tatctaattc caataattct tgttcattct tttcttgctg
     5401 gttttgcgat tcttcaatta aggagtgtat taagcttgtg taattgttaa tttctctgtc
     5461 ccactccatc caggtcgtgt gattccaaat ctgttccaga gatttattac tccaactagc
     5521 attccaaggc acagcagtgg tgcaaatgag ttttccagag caaccccaaa tccccaggag
     5581 ctgttgatcc tttaggtatc tttccacagc caggattctt gcctggagct gcttgatgcc
     5641 ccagactgtg agttgcaaca gatgctgttg cgcctcaata gccctcagca aattgttctg
     5701 ctgctgcact ataccagaca ataattgtct ggcctgtacc gtcagcgtca ttgacgctgc
     5761 gcccatagtg cttcctgctg ctcccaagaa cccaaggaac aaagctccta ttcccactgc
     5821 tcttttttct ctctgcacca ctcttctctt tgccttggtg ggtgctactc ctaatggttc
     5881 aatttttact actttatatt tatataattc acttctccaa ttgtccctca tatctcctcc
     5941 ttatggtgtt ttactaaact tttccatgtt ctaatcctca tcctgtctac ttgccacaca
     6001 atcatcacct gccatctgtt ttccataatc cctaatgatc tttgcttttc ttcttggcac
     6061 tacttttatg tcactattat cttgtattac tactgcccct tcacctttcc agaggagctt
     6121 tgctggtcct ttccaaagtg gatttctgct gtccctgtaa taaacccgaa aattttgaat
     6181 ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta tgtctactat
     6241 tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaattgt ggatgaatac
     6301 tgccatttgt actgctgtct taagatgttc agcctgatct cttacctgtc ctataatttt
     6361 ctttaattct ttattcatag attctactac tccttgactt tggggattgt agggaatttc
     6421 caggtctgaa gatcctgctg cttgctgtgc ttttttctta cttttgtttt gctcttcctc
     6481 tatcttgtct aaagcttcct tggtgtcttt tatctctatc ctttgatgca cacaatagag
     6541 ggttgctact gtattatata atgatctaag ttcttctgat cctgtctgaa gggatggttg
     6601 tagctgtccc agtatttgtc tacagccttc tgatgtttct aacaggccag tattaactgc
     6661 gaatcgttct agctccctgc ttgcccatac tatatgtttt aatttatatt ttttctttcc
     6721 ccctggcctt aaccgaattt tttcccatcg cgatctaatt ctcccccgct taatactgac
     6781 gctctcgcac ccatctctct ccttctagcc tccgctagtc aaaatttttg gcgtactcac
     6841 cagtcgccgc ccctcgcctc ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga
     6901 gagctcctct ggtttccctt tcgctttcaa gtccctgttc gggcgccact gctagagatt
     6961 ttccacactg actaaaaggg tctgagggat ctctagttac cagagtcaca caacagacgg
     7021 gcacacacta cttgaagcac tcaaggcaag ctttattgag gcttaagcag tgggttccct
     7081 agttagccag agagctccca ggctcagatc tggtctaacc agagagaccc agtacaggca
     7141 aaaagcagct gcttatatgc aggatctgag ggctcgccac tccccagtcc cgcccaggcc
     7201 acgcctccca tggtaatagc gatgactaat acgtagatgt actgccaagt aggaaagtcc
     7261 cataaggtca tgtactgggc ataatgccag gcgggccatt taccgtcatt gacgtcaata
     7321 gggggcgtac ttggcatatg atacacttga tgtactgcca agtgggcagt ttaccgtaaa
     7381 tactccaccc attgacgtca atggaaagtc cctattggcg ttactatggg aacatacgtc
     7441 attattgacg tcaatgggcg ggggtcgttg ggcggtcagc caggcgggcc atttaccgta
     7501 agttatgtaa cgcggaactc catatatggg ctatgaacta atgaccccgt aattgattac
     7561 tattaataac tagtcaataa tcaatgtcga cccaggtggc acttttcggg gaaatgtgcg
     7621 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     7681 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     7741 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     7801 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     7861 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     7921 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     7981 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     8041 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     8101 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     8161 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     8221 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     8281 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     8341 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     8401 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     8461 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     8521 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     8581 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     8641 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     8701 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     8761 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     8821 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     8881 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     8941 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     9001 tggcgataag tcgtgtctta ccgg
//
