LOCUS       sequence-407523-        9150 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, GOLGA3_SLC35E3 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9150)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_GOLGA3_SLC35E3
FEATURES             Location/Qualifiers
     source          1..9150
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(3911..4621,4622..4624,4625..4627))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3953..3974)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4302..4321
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4561..4582
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4624..4633
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4709..4912)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4738..4758)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4913..5216
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5473..5517)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5702..5935
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6295..6412
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6904..7029
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7076..7333
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7335..7714
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7745..7849
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7850..7918,7919..8710)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8068..8087)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8881..9150,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcaag
     1801 ggcgttgggc taatttacta cgactgcctt cctgctcgct caacttaaag tgcgtgtacg
     1861 ccaggatacc gaacagtgtg caaaggatgc ccagtgcctg gttgatgctt aagggatctt
     1921 taaacaggac gtagcctccg aacaatgtaa tacaaaattt gaagtgtccg aacatgttat
     1981 atgttactgg actagtgtta ccgataatcc aatagatact taaattgacc atgaaggcaa
     2041 ttacgccgga caaaagtacc attaactgga tgtgtgccat ctctccttgc aaacgcacac
     2101 gagcctcttg tgcaagagcc agctgttgtt ggtaccattg acgtacactt tgtaatgagg
     2161 agatttcggc ctgtgaggct ttcaattttg acgtaagcgt ggtgcgctcc aattgtacct
     2221 gttgtaactg tgactgtaac gcggtcatct gctgacgcaa gtcgtgcacc gtattatcct
     2281 tggaaagcat cgagcgttgc atgtcctcca ccttagccat caggcgctga tattgctcct
     2341 ccgcaacctg caggtcgttg ttactactcg caagcgaggc gtttttcgcc tccaacatat
     2401 tctgtaaatc tgtcatagcg cgttccagat cccagcaaga ttgtttcagt gtatcgactt
     2461 ctgacgaaag cgagtcttga cgttgctgac ttgaatggga gcattcaact tgggcctgta
     2521 actttgtaga caaggctgca agctgagcct gaagttctgc tttttctttc aaagcctgtg
     2581 aggcctcaag ggacaaggcc tccaattgcc cctccaagcg cattttttct tttaaaacct
     2641 gcagcatctc ttcttgggtc tcagccgcag acgattccag tgatacgctt gagcaaatac
     2701 tgtcgcggcg tgaacgaact tcgccattca cttcttggcc ctggtcctga tgttccgctg
     2761 cagcagcttg caaaacatcc ttaatactag ggaactgacc caacgtgtcc gctggaatct
     2821 cttgaccatt taccatgtaa ggtgtgtcct gagtgccgac cgttttgcta agaatgccat
     2881 aggtcccacg agtgctggct gaactataac tactactatc ggaatcgttc ccgtctactt
     2941 cgcttacgct atccccagca agagaggtat tttccaagcg gtcgtctgta tctggtgata
     3001 agctgatctc agagacaacg gatgccgccg aagagcgatt ctgcttcaaa ctgcctttcg
     3061 tagaatccgg cgcaggcacg ttaccaccgc tgtttccagc attggaatcc tcagtgcgat
     3121 aatctgccag cgaacggatc tttgacgatt tagaagtttt tttttcacgt gggtgggcgg
     3181 gcaagcctaa gctaccaacc tttggcccgc gtggaacaga ggtacgaaga aaagagtact
     3241 ccttagtcat tgccagtgtg gaagcgcggg gcaggttttc ggggttcagc attaattcag
     3301 gatctaaggt actgaaaagg cgggttttcg tagctggttg actagaacgt tcttgctgac
     3361 gcttaacgcg atattgtttt aactgttctt ctaaccactt gcgggcttgc aaacggactt
     3421 gttcctcttt ctcaaggggt aacgggctgt ccgtggaaca caattgtgtt tcttgcatag
     3481 gtaaagaaag acgcagactt tgcagagcct ccttacgtac ggaaccctcc gcagatgtcc
     3541 cttgagactt gcgaaggttg tcatggaagc ctgccacgcc aggacttgca tctggaccga
     3601 cgggcgaggt tgtgggatct aacgaagatg ggggatcggg gaagggcggc gtaggaccat
     3661 tctggcataa gcccccttgt cccgggccgt ccggcgactc tccttcagtg gaggcacggt
     3721 ttacctcggc gcactgaacc ttgtcttgtt ggtctggggg aactaaaggc cccggaggct
     3781 tcagaggcgc ttctggcagt gaagaaggac cggaatggct gcggtcctct tgcaggccat
     3841 cctgctcggc tgaagcccca tccattcccc gggaagcggc cgcacttccc ccggagcctc
     3901 cgcttccgcc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     3961 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc
     4021 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     4081 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     4141 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     4201 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     4261 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     4321 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     4381 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     4441 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     4501 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     4561 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     4621 tcaccatggt ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc agaattcggt
     4681 tcactaaacg agcccggttc actaaacgag ctctgcttat atagacctcc caccgtacac
     4741 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     4801 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     4861 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
     4921 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
     4981 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
     5041 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
     5101 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
     5161 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
     5221 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagcac
     5281 gcgttcgaag cttgagctcg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg
     5341 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc
     5401 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt
     5461 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc
     5521 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc
     5581 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca
     5641 actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc
     5701 caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt
     5761 gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt
     5821 gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga
     5881 cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc
     5941 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa
     6001 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc
     6061 tcctccttat ggtgttttac taaacttttc catgttctaa tcctcatcct gtctacttgc
     6121 cacacaatca tcacctgcca tctgttttcc ataatcccta atgatctttg cttttcttct
     6181 tggcactact tttatgtcac tattatcttg tattactact gccccttcac ctttccagag
     6241 gagctttgct ggtcctttcc aaagtggatt tctgctgtcc ctgtaataaa cccgaaaatt
     6301 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
     6361 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
     6421 gaatactgcc atttgtactg ctgtcttaag atgttcagcc tgatctctta cctgtcctat
     6481 aattttcttt aattctttat tcatagattc tactactcct tgactttggg gattgtaggg
     6541 aatttccagg tctgaagatc ctgctgcttg ctgtgctttt ttcttacttt tgttttgctc
     6601 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
     6661 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
     6721 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccagtatt
     6781 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
     6841 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
     6901 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
     6961 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
     7021 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
     7081 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
     7141 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
     7201 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
     7261 caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc
     7321 caggccacgc ctcccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
     7381 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
     7441 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
     7501 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
     7561 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
     7621 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
     7681 gattactatt aataactagt caataatcaa tgtcgaccca ggtggcactt ttcggggaaa
     7741 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     7801 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     7861 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     7921 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     7981 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     8041 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     8101 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     8161 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     8221 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     8281 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     8341 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     8401 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     8461 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     8521 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     8581 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     8641 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     8701 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     8761 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     8821 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     8881 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     8941 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     9001 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     9061 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     9121 tgccagtggc gataagtcgt gtcttaccgg
//
