LOCUS       sequence-407526-       13473 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, KIAA1549_BRAF from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13473)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 13473)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_KIAA1549_BRAF
FEATURES             Location/Qualifiers
     source          1..13473
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(8234..8944,8945..8947,8948..8950))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8276..8297)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8625..8644
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8884..8905
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8947..8956
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9032..9235)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9061..9081)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9236..9539
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9796..9840)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10025..10258
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10618..10735
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    11227..11352
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             11399..11656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        11658..12037
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12068..12172
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12173..12241,12242..13033)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12391..12410)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(13204..13473,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac tagtggaccg
     1801 gaaacgcacc atacccacct gcttgaatag gtgttttcgg agaggcgcac gcgtacaggg
     1861 agaagtcttc cgtctgaaaa ccagctctgt ttaaagacgg ttcggacgcg ctacgatgga
     1921 tcttcggcaa ggaacgagcc aacaattcta tggaggccaa gatctgcggg aagagtggtc
     1981 tttcatcacg tttttttttc aggcactctg ccatcagacg tttcatggcc ttcgggcagt
     2041 tagagcgaac cttgctcaag tccggagaca ggtaaccacg gccgaccatg aaaataatct
     2101 ggtcacggtt gttaatgttg ctgtacggca gctgaccggt catcagttcg tacaacacaa
     2161 taccgaaggc gtaaacatcg gactggaaag agtacgggtt tttgtcctgc atacggatta
     2221 cctccggcgc catccaaagg attgaaccgc tcagctgttc gaactggtgg ctgcccgacc
     2281 agcgactctt aacagtcgcc agaccaaagt caccgatttt cacggtaaga tcttcgtgca
     2341 gaaagatgtt gttactcttc agatcccggt ggataatact cttggcgtgc agataatcca
     2401 tgccctgagc ggtctgacgt gcaatatcaa tcagtttgat catctcgaac ttggtttcga
     2461 taatgtggag gtgatggtac agggaagatc cttcgcacca ctgggttacg atcgccaact
     2521 ggggtttcgt gctgtaaccc atgaataaaa gaatattcac gtgacgcgtc ttacgcaaca
     2581 ctccaacctc gtttttaaaa gcctgtagtt gctgcggggt cggagcggtc acgttcagca
     2641 ttttgacggc aacatcacca tgccatttac ctttgtacac ggtaccaaaa gaaccgctcc
     2701 cgatacgctg gccaactgtg atctgaccat ccggaatttc ccaatcatcg ctggagtccc
     2761 ggcgacccag cgttttcatg cgattgcgat cttcgctgct gctagagctt ttgcgttctc
     2821 tttgaggacc cgggcttttt tgcaacgcct taacgttcgt gagggaaccg ggcagcgacg
     2881 ccggcggggt tgcactcaaa cccgtcgtgc taccgccgtc accacggaag ccctgatcgc
     2941 gaatcaagtc gctacacggg ttgttcgcgg tctgtgccgg gctgtagaag ctaccccatt
     3001 gagtcgcgcg acgctcttct tgtgatgggg tgctgttcgc aggcaggccc ggtggcacac
     3061 ctggacccac gcccgcggta gacgctggtt gggagctcgg agccaccagt gcgaacgcat
     3121 catccagcaa gctatgcatt gtctgacgcg cctcctcgat tgacggttgt ggcgggatat
     3181 actgagaagc cggaaacggc agggccggat atcggccaag ctccacgctg ctcggcgtct
     3241 gtacgtcggc cggcaagtcc gggtcgctcg ggcagccaat gtacgcgctg ttatgcacgc
     3301 ccggtggacg acggtaggtg ccgtccgagt cggtggtgat cagacgatct ttttctgcat
     3361 ccgccgggca gccattaacc tggtgtttcc tgcgcggttt cgggctacgt ttaatacggg
     3421 agctcttgcg cggttcaata aaaacgctcg gcacgcttgc cgtcgggtcc aggattttat
     3481 caatttgcat ctgagcgcga cgatagacgc ggtggcgttc cttggtgtcg ccgctagaca
     3541 gatcgtccac aaccgggaac tcatagtgac cacgacgctt ggcacgcaga cgaatcttat
     3601 tacggtggtg ttcgatctca cttttgtggc gcagagcggt ctggatttct ttgttgattt
     3661 tgttgctttc cgcaacgcgg tccgccggag acgggcgctg cacaggcggc gccgggattg
     3721 gttgcattgc aattagctga atcttgctcg gaacccggcg cgaggcctcg ggcggacgag
     3781 aaatgcgatc gacgtgctca aaaatggaag cgcttgagtg ttgttcgttg ccagagctcg
     3841 gcaacggcgg accgctctgt ggcgcccggt ggctacggcc atcgttgacc gcgcccgggg
     3901 tcttatcgcc cgcatcacgc tcggaggatt cctcggaaac ggtagagtca gcgtcgctcg
     3961 gactcactct accacggtga cgcacgttct tggacgggat cttcgacttg ggcgacggca
     4021 cgtcaccgtt ttcgctcggg gtggtatgat ccttaagcgg tcccggcaac ggcgccggtt
     4081 catgaatgat caggatgtcg tccttgttgt gttggccgag atgctgcttc gcaaaatcga
     4141 aacccttcac agacgggatt tgcagtttct gacgctgctg gatgtttgcc acggtgtccg
     4201 gctggaagtc cagtttgtcg gtacggcata atttccagta caggatcacc acgataacca
     4261 tgaccacgag gacgggtatc actacgccca cgataaccca caggttattg ctttggctct
     4321 cagggctcgg acgtttaacg cgatccaccg gttgcgcgat cactccctgg atgcggtagc
     4381 ctaaaatgat cgccgcgcgc tgcagatcca tcttgttaat caggtcggat gatttaaccg
     4441 cggacaaccg ttcgccatcc tggtcttcaa caaagtaaat cagttgcacc ggattgtcgt
     4501 cgccctccag gcgagatacg ttgacaactt gaacgacaga attacctgct gcgacggtcg
     4561 cacgacgcca catacgacga cgggtcgata cttcggacaa gagttgcgcc agctttcgct
     4621 ccatctcggc ttggaacact tcgttctgca gttgtttctc cataacgccc agcagaactg
     4681 tacgaaccca agaagatttc aacagctgac tcaggttcag ttgaggatac tggaacggtt
     4741 ccgcaatttg cagaactgga tagcccaggt agaagctgaa ctccacgaca ctcaggttgc
     4801 gcagcagttc agacacctcg ctaccgttca gaaagccctg ggtgctcttc acagcaaaaa
     4861 tgatgttgac gggaccacgt ctaggcgtca cacggctgga ggagatcgtg atattcagga
     4921 tctgaacggt cagattgtat gtaccctggt gatgcttacg aacttcgaac agcgcggtca
     4981 tcagaccctt ttcgatacgt tgagtaaaat tgcagaaacc agtatcaacg cttggcggaa
     5041 caaactgtaa cacggtttga acttgaaagc ggctctccgg caccaggaag gacggcttca
     5101 cgctcaggat cagcggggtc tgatcgcgaa ccaacttgct gttgatcagc acattgatca
     5161 cgcttattgc ggtataaaca aaagggccac tcgtaaccag aaaggtaaag tcggtaaaca
     5221 gttcatacac cttcagttcg acagcgcggt taaagtggat acggagtact tctttaatcg
     5281 cggtaataat atactcttgg acagcacgac gtgcgagcac ggtggttatc aagtaggcat
     5341 ccggaacggt aatatcacaa acgtacggtg gcttagccgt tgctaaggtt ggcgtatcaa
     5401 cggttgcttc cagggtgaac gcggtcaccg gacgcagaga cggcaacggc ggtgcggccg
     5461 agctctcggg agggctttgg ctcgcagcgt ctcccatcag ggtcgagtcc agcggaccac
     5521 cagtcgctgc gccggtggag gtagtgctca cctcggtgga ggtattcagc gggacctccg
     5581 ttggcgttaa ggacgggcca accacggtca gttcggtcgg cagcggaaac ggggtggcct
     5641 cggacacgaa tgatgagccg ctcggcaggt atgcgtcggt aatcagaact gtaccagttg
     5701 ggatggcctt agagaaagaa gctagaacgc tgacgtgacc gtccaatgcg gaaatctggt
     5761 cgtcgggcgg ggtcagagta ctgaagagag aagactcggt cagaatcggc gtggtgtgaa
     5821 cggtggttaa cggagaggtc gcctgaatcc cagctgtcag gatgtcaaag ctctcgctac
     5881 ccggaggaac cagggcggta accgcgctct catgagaaat tagagagctt tccagatagc
     5941 tggtagtttc gatgaatgcc gatgtaaaat gggcctcact gtcagtgaga ctcaccgtgc
     6001 tcgcctctac aaactcaagg gagtcgctcg ggaagctgga ccacggagag acctcagatc
     6061 tgcttggcag cagcatgatc gtattaagcg gcagctcgct cgacggttgc aattggctga
     6121 actccaggtt cgtgctactc ggcaggctca attggctgga ctgtaaatca gagctatcga
     6181 acaacgtgaa ggtttcgact agtggggaga acgattggga ggtgaaagga gacaagtccg
     6241 acggcattaa agacaggctc gccggcgctt cggacgggga agaaatggaa ccgctcagct
     6301 ccagtggcgg ggtactaaaa aagctagacg cgaaatcgag agcgccccta ggtttgtgct
     6361 ctgagaagct ggacctttcc tgatctgaga acaggctgct ttcaacgctc gggaccaagc
     6421 tgtacggggt aaagacggac gggtcgcgaa ccgccagaga cggggtgttt ttgttcgcga
     6481 taacagagaa cgagctgtcc aacaatatgc ttgtgatgac actgaagaac gccgtggtca
     6541 cggaggacgg ggtcacctgc gtctcggcga cagacagcga acccgcagtc gggtccagag
     6601 acgccggaac actcagacga ccatgcgccg gcggaacctg ggtggtggta actgagctca
     6661 tgtccacctc tgcactgata cccacggagg tttcggagat ttccatactg cgactggaaa
     6721 gcggcacgat cggcctgctc ggaaacagag tattgaacac ttgcgggtcc tcttcaaact
     6781 ccgagaagtc tgcaacaacc gagctcatca agctaatgct cgactcctcc aatacggtca
     6841 tgcaaagggt ttcagcgccg tcgccgctac ctacgtcctt ctccatcagg gaagtggcca
     6901 acacttgttg cgggctcgga acggtgcacg cagcacacca ggtgtacggg cggaaagagc
     6961 taaccgggga gaggatatgg gtattatcga ccggacccgg caaggcgcta ttggagtgca
     7021 gctcggaggt cttggaggag tccgacggca gaaacgggtt agagctaaca tcggttgggc
     7081 ttgctgaaga ggcgaacgcc aagggggtgc tcagcagcgg gctgtgggta cgagagaatg
     7141 ccagcgctgc atgtgatgcg gtaacggtcg gataggtacg tggagaaatg gtttcctcca
     7201 gggattgcga taagacggta gtaacgtcgg tatatctgtc cgcagaggtt gcccacacct
     7261 cttccggcgg ttgtgacact tcgcccaagc tcgggagcgg aatggtgatg ccgtcaccca
     7321 ggggctgcgg cataaggcta cgtgacgaaa ggaaaagcgt ggtttcaacg ccctcggtta
     7381 ggctagcaac gatctccggc agggtacgcg acgatagatg gctgtacgcg tcggtcggat
     7441 agagcaccag gttgcgaccc ggcgtcggga caatgccctc gctggtgcga aaagcggaac
     7501 gaaaggtatg aaagtgggat gcgctctccg cgtacgccgc aggttggcgt gtcgtattct
     7561 gccagcctga agttacctcc tcatcctgaa gactgaccat cggcagagac ggagtcagca
     7621 tcggttccag cgcttcccgc ggcaagcccg gcgggctaac cgtgatatat tgaatcggag
     7681 acaccatacg tggggtggtc caatgggtgt ccggcagaaa attgtccatt tcatcatcat
     7741 ttactgcaac ctctttactg gtgacgctaa cgtaggtagc cacgaaaatg gatggatcgg
     7801 ctgtagactt ggtctgtttg ccctgattga aaaaggccgt gtcaaaagtg gtggcacttg
     7861 gcggagcagt cacgtgcagt ggggaagaat gttggctgcc tggggcagtt tccgtcagag
     7921 ccacctgggc cgcgctgtga cccgtagact tcttcagcac tagttccatt gaatacaagc
     7981 tcaggttgtg ctgctccggg ctcagttcat ccggcgcaca gctagcagga cgagccagca
     8041 gtaacagcca cagacccggc agcagaaggc cgggacgacg acgacgtgca caacgggcgc
     8101 tcggtctacg accgctcggg cccggggcca gagctacacc agcacgcggt ttgccttcca
     8161 ttgccgctcc tcgtctcctc cgacgagccc cgggcatagc ggccgcactt cccccggagc
     8221 ctccgcttcc gcccttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     8281 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagc ttggactggg
     8341 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     8401 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
     8461 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
     8521 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     8581 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     8641 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     8701 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
     8761 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     8821 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     8881 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     8941 tgctcaccat ggtggcgacc ggtggatccc gggcccgcgg taccgtcgac tgcagaattc
     9001 ggttcactaa acgagcccgg ttcactaaac gagctctgct tatatagacc tcccaccgta
     9061 cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt ggaaagtccc
     9121 gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga cttggaaatc
     9181 cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc atcaccatgg
     9241 taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat aaggtcatgt
     9301 actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg ggcgtacttg
     9361 gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac tccacccatt
     9421 gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt attgacgtca
     9481 atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt tatgtaacgc
     9541 ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat taataactag
     9601 cacgcgttcg aagcttgagc tcggtcccct cggggttggg aggtgggtct gaaacgataa
     9661 tggtgaatat ccctgcctaa ctctattcac tatagaaagt acagcaaaaa ctattcttaa
     9721 acctaccaag cctcctacta tcattatgaa taattttata taccacagcc aatttgttat
     9781 gttaaaccaa ttccacaaac ttgcccattt atctaattcc aataattctt gttcattctt
     9841 ttcttgctgg ttttgcgatt cttcaattaa ggagtgtatt aagcttgtgt aattgttaat
     9901 ttctctgtcc cactccatcc aggtcgtgtg attccaaatc tgttccagag atttattact
     9961 ccaactagca ttccaaggca cagcagtggt gcaaatgagt tttccagagc aaccccaaat
    10021 ccccaggagc tgttgatcct ttaggtatct ttccacagcc aggattcttg cctggagctg
    10081 cttgatgccc cagactgtga gttgcaacag atgctgttgc gcctcaatag ccctcagcaa
    10141 attgttctgc tgctgcacta taccagacaa taattgtctg gcctgtaccg tcagcgtcat
    10201 tgacgctgcg cccatagtgc ttcctgctgc tcccaagaac ccaaggaaca aagctcctat
    10261 tcccactgct cttttttctc tctgcaccac tcttctcttt gccttggtgg gtgctactcc
    10321 taatggttca atttttacta ctttatattt atataattca cttctccaat tgtccctcat
    10381 atctcctcct tatggtgttt tactaaactt ttccatgttc taatcctcat cctgtctact
    10441 tgccacacaa tcatcacctg ccatctgttt tccataatcc ctaatgatct ttgcttttct
    10501 tcttggcact acttttatgt cactattatc ttgtattact actgcccctt cacctttcca
    10561 gaggagcttt gctggtcctt tccaaagtgg atttctgctg tccctgtaat aaacccgaaa
    10621 attttgaatt tttgtaattt gtttttgtaa ttctttagtt tgtatgtctg ttgctattat
    10681 gtctactatt ctttcccctg cactgtaccc cccaatcccc ccttttcttt taaaattgtg
    10741 gatgaatact gccatttgta ctgctgtctt aagatgttca gcctgatctc ttacctgtcc
    10801 tataattttc tttaattctt tattcataga ttctactact ccttgacttt ggggattgta
    10861 gggaatttcc aggtctgaag atcctgctgc ttgctgtgct tttttcttac ttttgttttg
    10921 ctcttcctct atcttgtcta aagcttcctt ggtgtctttt atctctatcc tttgatgcac
    10981 acaatagagg gttgctactg tattatataa tgatctaagt tcttctgatc ctgtctgaag
    11041 ggatggttgt agctgtccca gtatttgtct acagccttct gatgtttcta acaggccagt
    11101 attaactgcg aatcgttcta gctccctgct tgcccatact atatgtttta atttatattt
    11161 tttctttccc cctggcctta accgaatttt ttcccatcgc gatctaattc tcccccgctt
    11221 aatactgacg ctctcgcacc catctctctc cttctagcct ccgctagtca aaatttttgg
    11281 cgtactcacc agtcgccgcc cctcgcctct tgccgtgcgc gcttcagcaa gccgagtcct
    11341 gcgtcgagag agctcctctg gtttcccttt cgctttcaag tccctgttcg ggcgccactg
    11401 ctagagattt tccacactga ctaaaagggt ctgagggatc tctagttacc agagtcacac
    11461 aacagacggg cacacactac ttgaagcact caaggcaagc tttattgagg cttaagcagt
    11521 gggttcccta gttagccaga gagctcccag gctcagatct ggtctaacca gagagaccca
    11581 gtacaggcaa aaagcagctg cttatatgca ggatctgagg gctcgccact ccccagtccc
    11641 gcccaggcca cgcctcccat ggtaatagcg atgactaata cgtagatgta ctgccaagta
    11701 ggaaagtccc ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg
    11761 acgtcaatag ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt
    11821 taccgtaaat actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga
    11881 acatacgtca ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca
    11941 tttaccgtaa gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta
    12001 attgattact attaataact agtcaataat caatgtcgac ccaggtggca cttttcgggg
    12061 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
    12121 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
    12181 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
    12241 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
    12301 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
    12361 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
    12421 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
    12481 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
    12541 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
    12601 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
    12661 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
    12721 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
    12781 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
    12841 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
    12901 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
    12961 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
    13021 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttaaaact
    13081 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
    13141 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
    13201 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
    13261 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
    13321 cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt taggccacca
    13381 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    13441 tgctgccagt ggcgataagt cgtgtcttac cgg
//
