LOCUS       sequence-407527-        9531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, ITSN2_ADCY3 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9531)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 9531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_ITSN2_ADCY3
FEATURES             Location/Qualifiers
     source          1..9531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(4292..5002,5003..5005,5006..5008))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4334..4355)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4683..4702
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4942..4963
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      5005..5014
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(5090..5293)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(5119..5139)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        5294..5597
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5854..5898)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6083..6316
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6676..6793
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    7285..7410
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             7457..7714
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7716..8095
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8126..8230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8231..8299,8300..9091)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8449..8468)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(9262..9531,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcacg
     1801 agttatcgac cacttggtgc ggtaaggtca cggatggacc attgggaaat gttgccaact
     1861 tgtcgcggcc cttcaagaag aaagtcaata actcaccttt cccttttaca aaaattggtc
     1921 cgcggcggac aaagcgaaac ccgtattcac gcaggataac ctgggtttcc tctacaactt
     1981 ggatgtttcc catgacgcct gtcgattcca tgcgggatgc cacatttaca gtatttcccc
     2041 agatgtcgta atgcggtttg cgggcgccaa ttacacccgc taagacgccg cctttgttca
     2101 tgccaatgcg aagcataaag ttattgaatg attgattgtt gatgttggtc agagtatcct
     2161 tcatcgccaa ggcgaagtcc gcaaggtctg ccagatgttg ccaacgctca cgctcagatt
     2221 tatcctcttt attagaggag gcaaaaccat tagtgtttac atcgggagtt accccagatg
     2281 ctgccatata tgtcgatccg atcgttttaa tcttagtgat tacgcggaac tttgggttgt
     2341 ccaacaacga atcgaaatcg ctgataatct cattcaagaa acgcaggcat tcgattccgc
     2401 cattgttaat tgactcttca gtgtagaaat ccgcgaagtt aggtaaagag gcgaacatta
     2461 ctccaatctc atcgtatgtt tgagagtaca actcctcgtc acgtttttta cttcctaaaa
     2521 aatgacgagc tacgtgctca ggtaacatat ttgtaaccaa cgcctcattc caacggcgca
     2581 tctcataaac acgctccttc tggtcgtgta cctcaatttt ccaaagaaac agagtacgag
     2641 ccagtttctc cacctgaagc tcttgctgaa gctgtttaat ctccataatc tccaggtcgc
     2701 actgtttgtc cagtacttct aattcggttt tttgcgtttg tttcttcaag cgaacgtcct
     2761 gtaaacggcc tgaaatttgt tggtgtttcc cgttcaaagc ctccagctca aggtgtaagt
     2821 ttttcttctt tgagttcaga cgtacaattt cttcctgctc acggttcttc tgattcagca
     2881 gttcttggcg gcggatacgt tcccactcca gacggcgttg gcgctctaac tcctgtttcg
     2941 ctgcttcgcg gcgctcaatg tctttgcgac gttcctcttc acgttggcgt tctaactcgc
     3001 gctgtttctc taagcgtttt tctaactcta actgtttttt ccattcttgc tcttgtaatt
     3061 cgcgttgctt acgttcccat tcctccttct ccttttgtgc cttacgctca gcctcacgct
     3121 gctgttgttc catcaaggct tgacgacgtt tttccagttc catattcccg cgctcatagt
     3181 tggccttgcg tttatcttcg aacgttacgg gcagcttctt ttggggttcc tcctcctgca
     3241 ttttttggta agagggaagg gtaccattga tactatcgat ttgtttaccc ccacggaagc
     3301 tgggaggaac caattccggc ggcaatgtaa gaggaagggg ctgcccagct ttagccatat
     3361 cggttaagtg cattgctaaa atgaactcct cagctttcaa ctgaccatct ccgtcaacgt
     3421 ccgctaatgt ccagatggtg gcaagctgtg tctgactaag attcgactgc aaaagggcat
     3481 tacgggcttg gaaacctgaa agataccccg acatgctttt gtcaagcgta ttaaattttt
     3541 ggcggtattt taagcgcgta ggttgaggaa ctgcccactc gctcgtgcca gtcttcgggc
     3601 tgtttccaga taacgaggcg gtcgatgaag tacttgagct cgaccccaga tcgatcagac
     3661 tctgcgcctt ttggatggat gctcccccaa acccacccat cattaaggag taactgctac
     3721 cgtggggaag cgtggatgag ctatatggaa ttggcaaggg ttgaattaaa cttgcggtgc
     3781 cattcggcag cgaggaagta gacacagaag gcaccagcgg ggtaggcatc atcaacgggg
     3841 gcagattagt cccacttgta gcacttgaaa gcgaggtgat gggtgcggcg ggaggaagcg
     3901 gctgcgggat gctaaggtta ggcattgacc ccatcccgaa acgcgcactg ataagaggag
     3961 agaacatcgg tggctgtttc ataatggggg gaagtacaac cggtaactgc tggccttgca
     4021 gctttaattt gatcaacttc atagcaattg aaaactcctg ctgatccatt ttgccatcct
     4081 tgttcagatc ggataaagcc caaatttctg caagtactgg tgccggcaaa ccactttgca
     4141 agaaaaaatt acgagcctgg tcgccggtga tgtagccccc cgatggcttc agattatcaa
     4201 actggcgatc atgtttggta cgttcttccg acgtaatcgc ccacattccc cgggaagcgg
     4261 ccgcacttcc cccggagcct ccgcttccgc ccttgtacag ctcgtccatg ccgagagtga
     4321 tcccggcggc ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt
     4381 tgctcagctt ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc
     4441 cgatgggggt gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgt
     4501 ggcggatctt gaagttcacc ttgatgccgt tcttctgctt gtcggccatg atatagacgt
     4561 tgtggctgtt gtagttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt
     4621 cgatgccctt cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc
     4681 gggtcttgta gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg
     4741 gcatggcgga cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg ctgaagcact
     4801 gcacgccgta ggtcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg
     4861 tgcagatgaa cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca
     4921 cgctgaactt gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg
     4981 tgaacagctc ctcgcccttg ctcaccatgg tggcgaccgg tggatcccgg gcccgcggta
     5041 ccgtcgactg cagaattcgg ttcactaaac gagcccggtt cactaaacga gctctgctta
     5101 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     5161 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     5221 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     5281 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     5341 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     5401 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     5461 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     5521 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
     5581 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
     5641 attactatta ataactagca cgcgttcgaa gcttgagctc ggtcccctcg gggttgggag
     5701 gtgggtctga aacgataatg gtgaatatcc ctgcctaact ctattcacta tagaaagtac
     5761 agcaaaaact attcttaaac ctaccaagcc tcctactatc attatgaata attttatata
     5821 ccacagccaa tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa
     5881 taattcttgt tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa
     5941 gcttgtgtaa ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg
     6001 ttccagagat ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt
     6061 tccagagcaa ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag
     6121 gattcttgcc tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc
     6181 ctcaatagcc ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc
     6241 ctgtaccgtc agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc
     6301 aaggaacaaa gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc
     6361 cttggtgggt gctactccta atggttcaat ttttactact ttatatttat ataattcact
     6421 tctccaattg tccctcatat ctcctcctta tggtgtttta ctaaactttt ccatgttcta
     6481 atcctcatcc tgtctacttg ccacacaatc atcacctgcc atctgttttc cataatccct
     6541 aatgatcttt gcttttcttc ttggcactac ttttatgtca ctattatctt gtattactac
     6601 tgccccttca cctttccaga ggagctttgc tggtcctttc caaagtggat ttctgctgtc
     6661 cctgtaataa acccgaaaat tttgaatttt tgtaatttgt ttttgtaatt ctttagtttg
     6721 tatgtctgtt gctattatgt ctactattct ttcccctgca ctgtaccccc caatcccccc
     6781 ttttctttta aaattgtgga tgaatactgc catttgtact gctgtcttaa gatgttcagc
     6841 ctgatctctt acctgtccta taattttctt taattcttta ttcatagatt ctactactcc
     6901 ttgactttgg ggattgtagg gaatttccag gtctgaagat cctgctgctt gctgtgcttt
     6961 tttcttactt ttgttttgct cttcctctat cttgtctaaa gcttccttgg tgtcttttat
     7021 ctctatcctt tgatgcacac aatagagggt tgctactgta ttatataatg atctaagttc
     7081 ttctgatcct gtctgaaggg atggttgtag ctgtcccagt atttgtctac agccttctga
     7141 tgtttctaac aggccagtat taactgcgaa tcgttctagc tccctgcttg cccatactat
     7201 atgttttaat ttatattttt tctttccccc tggccttaac cgaatttttt cccatcgcga
     7261 tctaattctc ccccgcttaa tactgacgct ctcgcaccca tctctctcct tctagcctcc
     7321 gctagtcaaa atttttggcg tactcaccag tcgccgcccc tcgcctcttg ccgtgcgcgc
     7381 ttcagcaagc cgagtcctgc gtcgagagag ctcctctggt ttccctttcg ctttcaagtc
     7441 cctgttcggg cgccactgct agagattttc cacactgact aaaagggtct gagggatctc
     7501 tagttaccag agtcacacaa cagacgggca cacactactt gaagcactca aggcaagctt
     7561 tattgaggct taagcagtgg gttccctagt tagccagaga gctcccaggc tcagatctgg
     7621 tctaaccaga gagacccagt acaggcaaaa agcagctgct tatatgcagg atctgagggc
     7681 tcgccactcc ccagtcccgc ccaggccacg cctcccatgg taatagcgat gactaatacg
     7741 tagatgtact gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg
     7801 ggccatttac cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt
     7861 actgccaagt gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct
     7921 attggcgtta ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc
     7981 ggtcagccag gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta
     8041 tgaactaatg accccgtaat tgattactat taataactag tcaataatca atgtcgaccc
     8101 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     8161 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     8221 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     8281 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     8341 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     8401 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     8461 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     8521 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     8581 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     8641 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     8701 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     8761 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     8821 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     8881 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     8941 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     9001 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     9061 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     9121 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     9181 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     9241 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     9301 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     9361 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     9421 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     9481 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg g
//
