LOCUS       sequence-407528-       14250 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, KMT2A_MLLT10 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14250)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 14250)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_KMT2A_MLLT10
FEATURES             Location/Qualifiers
     source          1..14250
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(9011..9721,9722..9724,9725..9727))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9053..9074)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9402..9421
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9661..9682
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9724..9733
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9809..10012)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9838..9858)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        10013..10316
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(10573..10617)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10802..11035
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11395..11512
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    12004..12129
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             12176..12433
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        12435..12814
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12845..12949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12950..13018,13019..13810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(13168..13187)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(13981..14250,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac taggactttt
     1801 cttgggcgac cggaccggtt ttgtctgaca agcgagcaac tttttgggat gcgttgtcac
     1861 cgtgaatcgt caaaaacggg ttcgtggtcg ccgtgtgtaa cggcggcgcc tgtgtacctg
     1921 ccagcaaatt gttgatcgga atctgcgttg gaccagggat ttgtagctgc tgcagctccg
     1981 gttggtgatg ctgctgatgg tggtgctgca ttaactggta cagaaacgct tggtgctgtt
     2041 ccggcgtcaa ttgctggcta ttgagcagct gctgaaattg ttgctggtgg atgagaatct
     2101 gacgttgttg gtccgacagc aagcccagac cagaggcgga attggtcagg gttgccggca
     2161 tcgggtgagt tgcattcggc ggaacggtcg tatgggtcaa tggggtaggg ttttggctca
     2221 tggtcaccgg ggtctgcata acgccgttga gagcaccgac tataccgttg atggccagct
     2281 ggttgccagg cagcgcgccg ataataccac cgaccgcact aacagcgggg atggtagagg
     2341 tcaccggctg catgccgctt gccagcgccc ccacggtcac tccattaacc tgttgcacac
     2401 cgctgacacc agagccctgt tgcgccggac tctgaccggc cggcggcggc gttgacagcg
     2461 cgctgctact agaggtagac tgcccgctag aggtcagatc ctgattcagg accgggaggc
     2521 tgttatcggg cagaaaggaa tttccgatat gcggtgactt agagctgttc aggctgtcgg
     2581 tagtcggcgc cgtttgcgca gaaaaggtgt ggatctggtg ggacggcgac gggttcgcgg
     2641 taatggtcgg gaatggcacg gacagttgcg cgttcagcag ctgcagacgc tccttcttgg
     2701 cggtcaagtt cttaatctgc tcttccagtc tacgattctc gacttgcaac tggtgcaggc
     2761 tcttcagcat gcccaggatg tcgctcgggg tgccttgctc aagcagaaac tgttgccctt
     2821 cgctccattg acgctccagc aactgctcta tagaggcagc caccggcggc aagttctcca
     2881 gcgagctatt acccggttga tcgtaacgga tttgcaacga gctgaccgga gaacgcgggc
     2941 ttaagctccc gcgtggtgat gagccgcggc cgaccagatt tctggaatta tcacccaaat
     3001 cctggtcggt ctggttgttc tcgctttggg cgatgagtgc tgccatgctg gagttagaac
     3061 gattgccata catgtggctt ggggcctgag acaggctgga cccggacagg gtattcgcct
     3121 gggtggtcgc aactgcgggg gccgcgctgc tcacagcgct cggagacagc gctccaactt
     3181 gttgtagatg accagagctc tgctgtggca ggtgtgacga ggatactggg gtgctagagc
     3241 cgctgccgct aacaacattc gggaaggaga cagcgacatc gttagagtta taaataccat
     3301 tatggattgc attcagcagt tcggagaatg tcgtggtcgg gcacgcacca tcgtgacgat
     3361 attgcatact gatttcgctt cctaccgggg aggaaccaac agagaggctc tggagtgaac
     3421 cgttacggag aagggtcggg gacttctgca gagaggatga ggttatacta cccgcagcgc
     3481 tggccaccga gctggtcggg ctcgctgatg acacagacag gtggctcacg ttttcttggt
     3541 tcttgttgcc ctttgggcga cccggcccgt gcttactctg tttgttaccc ttacgcttct
     3601 tctctttcac ggtttcctct tcaatgccgg aggtttgggc gcgtttataa cccgcagtcg
     3661 ggagggagct gtttcccaga tcgcccgggg agttttcgaa agatttcggt tgactggtca
     3721 ccgctgaggt ggatggcaga ccaatcaagg tagagaaaga gttcacgcca ccctcttgtg
     3781 aaccactctc acccttatgc acgtccttgg tcgcggagga ctgctggctg tgagaatagc
     3841 tgtcattacg caagtctgag tcggtgaagc tcaggaaatc ttgtggactc tggacactgc
     3901 tgccggagct gctcttcaca gaacccgggg tgccggagaa gctaccctgc gggaacggtg
     3961 aggccgcgga caccgttgta cccggattac ggccaccgcc cggcttgcgg ccacgctgac
     4021 cagaagaatg cgccgaggac tttttacctt taccttcaga gccgcgtgtc tcgctaacat
     4081 ctttgccgga actggtgtgg gcggacactt cctgaaagtt agcattggta aaacgagccg
     4141 tggtgtcctc caagcgctta agggaaccgc taattgagtt attggaagtg ctggtgtacg
     4201 ttttttctgt agtaaccgtc agggatggaa ccagcgccgg ggaaggctcg ggctgctttt
     4261 tatgcttctg tttgtgcttg tctttctctt tgtatttttt tttttctttt tcatggtgtt
     4321 tatcctgaga gtgagaactg ctatcgctca gggactggtc gtagctgcgg ttgctgcctc
     4381 ttttggactt cttcaatttg ctaaaatggt acttgcaata accgcagtac tgaacattat
     4441 ctgcgccatt cccctcttct tcgcaaagca gacccgcaaa ctgggcgcac gtaacatgaa
     4501 acgcttgacg acagccgtgt ttattgcagg tcatacacgc acccgtagcc gccttgctct
     4561 cacgcccctg ttcatcacaa atatagcaag ttttattgta tctatcatgc gggacggatt
     4621 gcagaacgat aggttccatc gtactcacgt ttgcgaactg gacttccgga atgtatagtg
     4681 cgcacacaac atgtgcccag ccgccgttgt cagtacgctt cagcgcgccg tccttgtgcg
     4741 ggcacagttc acaacgttta aaatcgacac gaatacgatg aacgccatca gccgggattt
     4801 tctgcttaga cgagttacca ttactgagcg tggacaggat gttcagcgtg cctgcgtttt
     4861 cttgcttatt aaccgggggg ggtttctctt tttcttttgg tttttgtttc accggtattg
     4921 acggacgcgg agccactttt ttttgcttac tttgttccgg accgctctcc ggcggcggcg
     4981 gctgcttctt tttcggctcc gacggggtgg ttttcggcac ttccttacgc ggcggcccag
     5041 tggtcggcgg ctgcggcgga ataacgagag ctggctggct gacctgcttg ctggatttgc
     5101 ggctcgctgg tgtggtcgct tgtttactct cgggacccgg ggccgaaacg ttaccctcct
     5161 cggatttctc ttcaaccggt ttccgcggtg gcggctcgct ggagctcttt ttcggtgccg
     5221 ggtcctcacg cgcgctcgga gtcggctttt gggatgagtc cacgacgttt ttcacgacag
     5281 agctttcctt ggaatccttc ttttcagagg ttttcgactt tttctccttc ttcttcacag
     5341 ctttcgcctg tttctgcaga tacgctttgg acggcatcca ttgcaagttc tggcacttac
     5401 gcattttaca acactgcttc ttaatgttac gaccaccaaa ttttggttta tccaggcagt
     5461 tggtgcagac gccacagtcc tctggcacct ggcagcccgg acattggccg cagcgtcggc
     5521 tccgacggcc tttcttcacc ggtggttcct gcggtgcctt gttacgcgtc accggtttaa
     5581 tcggcttgat cggcggtgcc agcggctcag cgtcctctga gccggctatg gagcttttgt
     5641 cgtcgttacc catagagctc aggatcttct cacgctcttc ccacggcagc gctgacaagg
     5701 tcggcatatc atctgggaac acggcgcgct tacggcccag cgcgaccgcg gcacgacggc
     5761 acacgtgctt aatacgcggg cctctaacgc ttgtctcaga gctatcagac tcttgacctt
     5821 gcgccttcgg ctggtcagtc tgtttcaggg acttgctctt ctcgattttg caaagctggg
     5881 ctttggcctt cttaagcaga gaggccacgc gcttatcggt catcggcagc ttatctgctt
     5941 gagccagcat gctaccgatc gaggaggtgc tatgcttaac ggttgaagat gatggcgtac
     6001 tcaggcacag ggtcttttct ttttctagtg acggtgccgt cggacccaga tccagattgg
     6061 ttttctccaa gttgccgcga ccctttttga tcagaatctt ggtcttaacc gccgtggtat
     6121 cgcccaaggt cacagaggtg atatcggtac cgctatcgtg ggaagaagat ttcttacggc
     6181 ccgtcgcctt cttcgcactg ctgctggtcg caacgtcttc accaacaact ttttctttgg
     6241 aaacgcggcc gaccgggtac aatgcactag aggactgaat ctcgctaccc ttcttacgct
     6301 tctccttgcg actctctcgt ttgttctcct tttctctctc acgatcacgt tcgcgacttt
     6361 tgtccttttc aacagatttg tctgcatctc tgtccttaga cagttcttcc ggagctttat
     6421 ccttgttgcg gcccctttcg gtctggctgc ccggggtaaa ccaaggaaaa agcggggtcg
     6481 gcgagctaga ggagaacggt tccgcaggcg ctgaggtttg cttacgcgga cgttggtttt
     6541 tttcggcgct ttcaccgctt tgtgtcaggg agtgagacgg gaaggtaaag gtcggattca
     6601 gagcgcttgt ggccaacggg gaaacgctga tagataatga tgagctcacg gaactcggcg
     6661 gggtcagggg agagagctcg ctactagata aacggcccga acgggtacgc atactgtggc
     6721 tagggctacg cggctcggaa cggatcggtg aaaacacttt acgcttgcgc ttgcgattag
     6781 aaacacccga gctgctggta ccagcagagg tacgattact cggcagggtc acggattcaa
     6841 agatccggga gtgggcttcg ctcggggtga agcggggcgc tcttaacagc ggggagcgtt
     6901 tatgcatgtc gaaacgggtg ccactgtgca gcggtgagaa caaacgcgct gacgctgcgg
     6961 tgccgctagc tgagaaaccg ctcgcaaaac ccacatcttc cggcgtgaga gggggcggac
     7021 gaaaattgtc aaaaatcggc ttgcggatca ggccctcctt ggcatacttg gcgctagaga
     7081 agtactgcgg ctccgaacgg ctatgtttca gtgaggtcca acgaaaggtc ggttcacgca
     7141 ggatgctttt gcgtttaccc tgcatcggtg cagtgctcgc gggcagaaat ggggaagcta
     7201 acgggatggt aggcggcatc agccacgggg tatgatcgct aatactggaa gcaggttgca
     7261 gcggtggggg cggggtcagc aaaggtgggg gcggcgaaga agaagtttgt tgctgcggag
     7321 cagactgcag agttgatagt tttttggtgg ttctggaacc gaacgaacgc tccgaaacgc
     7381 tgtaacgacg ggaacgtctg tcgttgcttt cattttctgg gctttggcta atcggcagcg
     7441 gcggatgcac ctcgggggtg tcggagcgct cctccggcag aacctggatc tcctcagagg
     7501 cctgggagtc ggtgctggta tccacagacg gggaactgct acgggacgag tcgctgctca
     7561 tttggctgct gtgttgggag gccgcggagc ttttctcaga actaccacat gaaggcgcgc
     7621 tgaaacggct gttcggcgta gactccagtc tggcgatttt tatcggcgga tcatagtctt
     7681 cgtcttcgat gaaacgacgg ggtgtcttaa tgatacggct gctgattgct gaaaccaccg
     7741 gcataatgaa ttgacgaatg tttttaactt gcgtcttcac ctttctgccc tgcagttgag
     7801 cagcttcttt ttcgatcttt ttctgggccc cctttttcgc acgttgcagc aactgtttcg
     7861 cgatcgttgc atccgtacgt ttcgaggatg ggattatgcg aaccggttta atgcgccgcg
     7921 gtgattgacg cactacggtc ttgtcctctt tcgtcagcgg aggcgtaccc tctttgtcct
     7981 tacgcacttt ctgcggcttc tccaactcgc tgttaatcag taagcctgat ggggttttaa
     8041 tgcgttcggt actcggcggt ctgccacggc gacgaacaat ttgaacaccc ttccgaccga
     8101 tttgaagttt accagtctta aacttggact tcaacggact caatttgccc gcacgcagtt
     8161 tcttaatttt cgttgcttgt tgaaaggtcg cgctcggcgt acgtttgatt tttttcagac
     8221 tgtcctcttt attaccttta ggcagctctg agatgtcttt accatgggtg atcttgattt
     8281 taactccggg gaaggtaggc ggacggcctc gtttcttttc gatactcttg ctgtccttct
     8341 tcttaatttt gtcgccactt ttggtctcgc ttttgttgag cggggagaaa acgctcgggt
     8401 cgctcaagat cgcagagtta cggtcgctgc cagatcttgg acggccacgc ggtttacgcg
     8461 gtgacgtctt gacagaaggg ctgcgcgtcg gggagcggac tcgcacttct tcgtcggaac
     8521 cgaagcccag aaactgctca tcttcaccgc taccaccgcc accacccgat tcaccgaaaa
     8581 ccgcacgaaa acgacgcagg ttggtgccga tagccgcaga gacctgcagc gctgcatcga
     8641 agcccggacc cacgcgcagc agtgcaggcc cgctgcttgc gctagaggag ctagaggaag
     8701 agctgctggc cgagctggag gatgccgccg aagcagccgc agcgccaccc ggaacaccgg
     8761 cgcctgagga acccgcagca gctgctgccg ccgcgaccgc tggaggcgac ggcggtgcac
     8821 ccgggccgcc accgcctacc ggcggacctg ggggtaacag caacgccggg acgcgctgac
     8881 ggggcgcgcc tcccaggcca cgacgaccac cgccaccgcc tccgccagtg gtccccggac
     8941 gcgcgggaaa cctccatcta catgagtgag ccatagcggc cgcacttccc ccggagcctc
     9001 cgcttccgcc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     9061 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt gctcagcttg gactgggtgc
     9121 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc gatgggggtg ttctgctggt
     9181 agtggtcggc gagctgcacg ctgccgtcct cgatgttgtg gcggatcttg aagttcacct
     9241 tgatgccgtt cttctgcttg tcggccatga tatagacgtt gtggctgttg tagttgtact
     9301 ccagcttgtg ccccaggatg ttgccgtcct ccttgaagtc gatgcccttc agctcgatgc
     9361 ggttcaccag ggtgtcgccc tcgaacttca cctcggcgcg ggtcttgtag ttgccgtcgt
     9421 ccttgaagaa gatggtgcgc tcctggacgt agccttcggg catggcggac ttgaagaagt
     9481 cgtgctgctt catgtggtcg gggtagcggc tgaagcactg cacgccgtag gtcagggtgg
     9541 tcacgagggt gggccagggc acgggcagct tgccggtggt gcagatgaac ttcagggtca
     9601 gcttgccgta ggtggcatcg ccctcgccct cgccggacac gctgaacttg tggccgttta
     9661 cgtcgccgtc cagctcgacc aggatgggca ccaccccggt gaacagctcc tcgcccttgc
     9721 tcaccatggt ggcgaccggt ggatcccggg cccgcggtac cgtcgactgc agaattcggt
     9781 tcactaaacg agcccggttc actaaacgag ctctgcttat atagacctcc caccgtacac
     9841 gcctaccgcc catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt
     9901 gattttggtg ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc
     9961 gtgagtcaaa ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa
    10021 tagcgatgac taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact
    10081 gggcataatg ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca
    10141 tatgatacac ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac
    10201 gtcaatggaa agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg
    10261 ggcgggggtc gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga
    10321 actccatata tgggctatga actaatgacc ccgtaattga ttactattaa taactagcac
    10381 gcgttcgaag cttgagctcg gtcccctcgg ggttgggagg tgggtctgaa acgataatgg
    10441 tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaacta ttcttaaacc
    10501 taccaagcct cctactatca ttatgaataa ttttatatac cacagccaat ttgttatgtt
    10561 aaaccaattc cacaaacttg cccatttatc taattccaat aattcttgtt cattcttttc
    10621 ttgctggttt tgcgattctt caattaagga gtgtattaag cttgtgtaat tgttaatttc
    10681 tctgtcccac tccatccagg tcgtgtgatt ccaaatctgt tccagagatt tattactcca
    10741 actagcattc caaggcacag cagtggtgca aatgagtttt ccagagcaac cccaaatccc
    10801 caggagctgt tgatccttta ggtatctttc cacagccagg attcttgcct ggagctgctt
    10861 gatgccccag actgtgagtt gcaacagatg ctgttgcgcc tcaatagccc tcagcaaatt
    10921 gttctgctgc tgcactatac cagacaataa ttgtctggcc tgtaccgtca gcgtcattga
    10981 cgctgcgccc atagtgcttc ctgctgctcc caagaaccca aggaacaaag ctcctattcc
    11041 cactgctctt ttttctctct gcaccactct tctctttgcc ttggtgggtg ctactcctaa
    11101 tggttcaatt tttactactt tatatttata taattcactt ctccaattgt ccctcatatc
    11161 tcctccttat ggtgttttac taaacttttc catgttctaa tcctcatcct gtctacttgc
    11221 cacacaatca tcacctgcca tctgttttcc ataatcccta atgatctttg cttttcttct
    11281 tggcactact tttatgtcac tattatcttg tattactact gccccttcac ctttccagag
    11341 gagctttgct ggtcctttcc aaagtggatt tctgctgtcc ctgtaataaa cccgaaaatt
    11401 ttgaattttt gtaatttgtt tttgtaattc tttagtttgt atgtctgttg ctattatgtc
    11461 tactattctt tcccctgcac tgtacccccc aatcccccct tttcttttaa aattgtggat
    11521 gaatactgcc atttgtactg ctgtcttaag atgttcagcc tgatctctta cctgtcctat
    11581 aattttcttt aattctttat tcatagattc tactactcct tgactttggg gattgtaggg
    11641 aatttccagg tctgaagatc ctgctgcttg ctgtgctttt ttcttacttt tgttttgctc
    11701 ttcctctatc ttgtctaaag cttccttggt gtcttttatc tctatccttt gatgcacaca
    11761 atagagggtt gctactgtat tatataatga tctaagttct tctgatcctg tctgaaggga
    11821 tggttgtagc tgtcccagta tttgtctaca gccttctgat gtttctaaca ggccagtatt
    11881 aactgcgaat cgttctagct ccctgcttgc ccatactata tgttttaatt tatatttttt
    11941 ctttccccct ggccttaacc gaattttttc ccatcgcgat ctaattctcc cccgcttaat
    12001 actgacgctc tcgcacccat ctctctcctt ctagcctccg ctagtcaaaa tttttggcgt
    12061 actcaccagt cgccgcccct cgcctcttgc cgtgcgcgct tcagcaagcc gagtcctgcg
    12121 tcgagagagc tcctctggtt tccctttcgc tttcaagtcc ctgttcgggc gccactgcta
    12181 gagattttcc acactgacta aaagggtctg agggatctct agttaccaga gtcacacaac
    12241 agacgggcac acactacttg aagcactcaa ggcaagcttt attgaggctt aagcagtggg
    12301 ttccctagtt agccagagag ctcccaggct cagatctggt ctaaccagag agacccagta
    12361 caggcaaaaa gcagctgctt atatgcagga tctgagggct cgccactccc cagtcccgcc
    12421 caggccacgc ctcccatggt aatagcgatg actaatacgt agatgtactg ccaagtagga
    12481 aagtcccata aggtcatgta ctgggcataa tgccaggcgg gccatttacc gtcattgacg
    12541 tcaatagggg gcgtacttgg catatgatac acttgatgta ctgccaagtg ggcagtttac
    12601 cgtaaatact ccacccattg acgtcaatgg aaagtcccta ttggcgttac tatgggaaca
    12661 tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg gtcagccagg cgggccattt
    12721 accgtaagtt atgtaacgcg gaactccata tatgggctat gaactaatga ccccgtaatt
    12781 gattactatt aataactagt caataatcaa tgtcgaccca ggtggcactt ttcggggaaa
    12841 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
    12901 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
    12961 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
    13021 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    13081 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    13141 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
    13201 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
    13261 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    13321 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    13381 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    13441 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    13501 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    13561 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    13621 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    13681 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    13741 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    13801 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    13861 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    13921 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
    13981 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
    14041 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
    14101 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
    14161 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
    14221 tgccagtggc gataagtcgt gtcttaccgg
//
