LOCUS       sequence-407529-       13065 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, KMT2A_MLLT1 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13065)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 13065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_KMT2A_MLLT1
FEATURES             Location/Qualifiers
     source          1..13065
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(7826..8536,8537..8539,8540..8542))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7868..7889)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     8217..8236
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8476..8497
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8539..8548
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(8624..8827)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8653..8673)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8828..9131
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(9388..9432)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9617..9850
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    10210..10327
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    10819..10944
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10991..11248
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        11250..11629
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        11660..11764
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(11765..11833,11834..12625)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11983..12002)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(12796..13065,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac tacgtcgcaa
     1801 ccgcctccag acagctttgc agtttcctca ctgtggtctc gtccagagaa aacaggtcga
     1861 aatcaaaggt ggtgttggtc acgttaaagt ggccggtttc ttcaatcaga ttcacaatct
     1921 gttgcagcac gttgcgctct ctcagtgcca taaggcgacg gtgcaattca accaactcgt
     1981 cggtgtacgc tttgtcgtac gtccctttct ttaagatctt ctccggcttg ctgcagctct
     2041 cagggctacg acggccgctc accttgctat ttggcggcgg cggcttctga ggcggaggtg
     2101 gctcacgaga tggcaggcta ctgtccgcgg agttgtcgct ttcactatcc gagaacgaga
     2161 gcctgctgtc acgacccggg ttggttttgc ccgcggcctc ctcgcctgag ctcgaatcgt
     2221 cttcatcgga ctcctcactt tgcaggtcct ctaccatgct tctcaacggg ccctggctgt
     2281 ggttttgcga gggttcaaag tcagagtccg acgagctgtc gctagaactg ctagagttgc
     2341 tcggtgatga ttgcgcagat tctgatttga agctcgcttc atcttcgcta ttactctctt
     2401 caacctctaa agcctttttc gcctcacgcg gctcggattc tgccttcact ttctcaccac
     2461 gggtgctaga cttgtcctta gccggctttt tatcagagaa tgaactgcta gaagaagtac
     2521 gaggagatgt acctggggcg gaacgtgagc ctttggaaga gctctttttc tgcttcttcg
     2581 cactcggttt cggggagtca gccgtcgccg gccgctttga ggatgcgcgt ggcggcggtg
     2641 gcggcggtgg cggcggtccg ccttttggcg aagttgattc cagtttggtc tctttcaggg
     2701 ccattttggg ctccttaaac gccgccttgg gcggcggtgc cttttcttct ttcggcaatc
     2761 taccctcacc aagtttacga ctggtatcct tggagctttt cgcttgttcc ctctccagct
     2821 ctttcgaaga cgatttcgac tcggaatctt tccgaggacg ttcgcgatgt tctttggtaa
     2881 ctttatgcgg ttttgaggtc ttcgagcttt ctttatttgc atctttgctg ccgtgtgacg
     2941 gcttggtctt tttcgggtca gagaacgcac tcagcgggat cgtcggcagc atcgggtaat
     3001 cggggctcgg acgggacacg gtatcggcac cctccggcat aaccatgaca ccgcccgcac
     3061 gaagcaattt gtaacggaac tcggtggtcg ggttattgaa cgtcaatttt tcacagcgca
     3121 gatggttgac cggcgggtta ccttccaaat tcagaaacag atcataggta aagcagactt
     3181 tacgtggctc ctctttgttt ttgaagtgaa cctcgatggg cattatgaag cctgcatagc
     3241 cggattcttc aactttatac ggcggttctt tacagacccg gcgcggcttc gggaagctgt
     3301 cgtgcagcca gaaaaccacc ttttcaacaa aatgctggat atcacactgt tccggaccgc
     3361 gcacgaaaac catccagtca tgggtgaacc cttcggtagt gggcttcttg cgcagttgag
     3421 cacggtggcc cagctctaag cgcacctgaa cggtacattc aacatggcct gatgatgcgc
     3481 aaaggaagca aaccacacgc ggggtgatcg gtacggaggt cagaatacca agacctccca
     3541 tttcccaaac gttttccgct tcgcaatcct ccttgaagtc cacacgaata cggtgcacac
     3601 catccgccgg aattttctgc ttgctgctgt taccattgct cagggtgctc aaaatattca
     3661 aggtacctgc attttcttgt ttattcactg ggggcggctt ctccttctcc ttcggctttt
     3721 gcttaaccgg gatgcttggg cgcggtgcga cttttttttg cttactttgc tctggtccac
     3781 tttcaggcgg tggcggctgc tttttttttg gctccgacgg cgtagtcttc ggaacttctt
     3841 ttctcggcgg acctgtcgta ggtggttggg gcggaatcac cagcgccggc tggcttacct
     3901 gtttggagct cttgcggcta gcgggcgtcg tcgcctgttt agactcaggg cccggtgcgc
     3961 tcacgttgcc ttcctccgat ttttcctcca ccggcttccg cggaggtggt tcggagctac
     4021 ttttctttgg tgcagggtct tctctagctg acggggtcgg cttttgactc gaatccacaa
     4081 cgtttttcac cacgctgctt tccttggagt cctttttctc gctcgtcttg gatttctttt
     4141 ctttcttctt aacggccttc gcctgcttct gcaggtacgc tttgctaggc atccattgca
     4201 gattttggca ctttcgcatt ttacagcatt gttttttaat gttgcgaccg ccaaatttcg
     4261 gtttatccag gcagttggtg cagacgccac agtcttccgg cacctggcac cccgggcact
     4321 ggccgcagcg gcgggatcga cggccttttt taaccggcgg ctcttgaggc gctttgttac
     4381 gagtaactgg tttaatcggc ttgataggcg gcgcgagtgg ttctgcatct tcggaaccgg
     4441 cgatggaaga tttatcatcg ttacccatgg agctcaagat tttttcacgt tcctcccacg
     4501 gtagtgcgct gagagtcggc atatcgtccg ggaaaacagc tctcttgcgg cccagtgcca
     4561 ccgctgctcg acggcacacg tgtttgatgc gtggacctct aacggaagtc tctgagctat
     4621 cagattcttg accttgcgct ttcggctgat cggtctgctt aaggcttttc gacttctcga
     4681 ttttgcacag ctgcgctttg gctttcttta acaggctcgc tactcttttg tcggtcatcg
     4741 gcagtttatc tgcctgggcc agcatgctac cgatcgatga ggtgctgtgc ttcacggtcg
     4801 agctactagg cgtgctcagg cacagtgtct tctccttttc cagacttggc gcggtcggac
     4861 cgagatcaag attggtcttc tccaggttgc cacgaccctt cttaatcagg attttggttt
     4921 tcactgcggt ggtatcacct aaggtcacgc tagtaatatc cgtaccggaa tcgtggctgg
     4981 aggatttctt acgaccggtc gcctttttgg cggagctgct ggtcgccacg tcttcgccca
     5041 caaccttttc ctttgacaca cgacccaccg gatacagagc tgatgagctt tgaatctcgg
     5101 agcctttttt gcgtttctcc ttacgagatt cacgtttgtt ctccttttct ctctcacgat
     5161 cgcgctcacg gctcttgtct ttctccactg atttgtcagc atctcggtct ttgctcagct
     5221 cttccggggc tttgtctttg ttacgaccac gctcggtctg gctgcccggg gtaaaccacg
     5281 ggaaaagcgg cgtgggactg gaggagctaa acggctccgc aggcgcgctc gtttgcttgc
     5341 ggggacgctg gtttttttca gcggactctc cactctgggt caaactgtgg gacgggaaag
     5401 tgaaggtcgg attcagagca ctagttgcca ggggagacac actaatgctc agtgaagagg
     5461 aaacactgct cggtggggtg agcggggata attcgcttga gctcaaacga ccgctacggg
     5521 tccgcatgct atgggacggg gatctcggct ccgaacggat cggggaaaaa accttgcgct
     5581 tcctcttacg gttggacacg ccactgctac tggtaccggc agaggtacgg ttgctcggca
     5641 acgttacaga ttcaaaaata cgactgtgag cctcggacgg ggtgaaacgc ggagcccgga
     5701 gcaacgggga tcttttatgc atgtcgaagc gagtaccgga gtgcaggggg ctaaataaac
     5761 gcgctgacgc ggcggtgcca gatgcgctga aaccagaggc gaagccaacg tcctccggcg
     5821 tgagcggcgg agggcgaaag ttatcaaaga tcggcttacg gatcagaccc tccttggcat
     5881 acttcgcaga agaaaaatat tgcggctcgg atctgctgtg tttcaagctg gtccagcgga
     5941 aggtcggttc acggagaatg gacttacgct taccctgcat aggagcggtt gacgccggca
     6001 aaaacggaga tgccaacggg atcgtcggcg gcatcagcca cggggtgtgg tcgctgatag
     6061 agctggccgg ctgcagtggc ggcggcggag ttagcagcgg gggcggcggg gaactagagg
     6121 tctgttgttg cggcgcgctt tgcagtgttg acagcttttt agtggtacga gagccgaagc
     6181 tacgctcgga aacgctgtag cggcgagagc gacgatcatt gctttcattc tccggagatt
     6241 ggctgattgg aaggggcgga tgcacctccg gggtatcaga acgctcctcc ggcaggactt
     6301 ggatctcctc gcttgcctgg ctgtcagtac tcgtatcaac gctcggggat gaggaacgag
     6361 acgaatcaga gctcatttgt gaggaatgct gactcgcagc ggaagacttt tcggagctgc
     6421 cacagctcgg agcgctaaag cggctgttcg gggtagattc cagacgcgcg attttgatcg
     6481 gcggatcgta gtcttcgtct tctatgaagc gacgaggggt cttgattatg cgggagctga
     6541 tggcagaaac caccggcata ataaactgac gaatgttttt aacctgggtc ttaaccttac
     6601 gaccctgcaa ctgagcagct tctttttcga tctttttctg tgcacccttt ttcgcccttt
     6661 gaagcagctg ctttgcaatg gtagcatcgg tacgtttgga cgacgggatg attctaaccg
     6721 gcttaatgcg acgagggctc tggcgcacaa cggtcttgtc ttctttggtc agcggagggg
     6781 tgccttcttt gtccttccta accttctgcg gtttctccag ctcggagttg atcagtaggc
     6841 cagacggggt cttgatacgc tccgtagacg gcgggcggcc acgacgacga acgatctgaa
     6901 cacctttacg accgatttgt aacttgcctg ttttgaactt agacttcaac gggctcaact
     6961 tccccgcacg tagcttcttg atcttcgtcg cttgctgaaa ggtcgctgac ggggtacgct
     7021 taattttttt taaagagtcc tctttattgc ccttcggcag ctctgagatg tctttaccat
     7081 gggtgatctt aattttcaca cccggaaacg tcggcggacg accacgcttc ttctcgatag
     7141 atttagaatc cttcttctta attttatcac cggattttgt ctcgctctta ttcagcggag
     7201 aaaacacaga cgggtcgctc agaatcgcag agttacggtc ggagccgctg cgagggcgac
     7261 cccgcggctt gcgcgggctg gtcttcacgc tcggggatct cgtcggagac cggacacgaa
     7321 cctcttcgtc ggacccgaaa cccagaaact gctcgtcttc gccgctacca ccgccaccac
     7381 cgctttcgcc gaacactgcg cggaaacgtc taaggttggt gccaatcgct gcgctgactt
     7441 gcaaggctgc gtcgaagccc gggccgacgc gcaagagtgc tggaccgcta gatgcactag
     7501 aagacgatga cgatgaggaa gacgcgctgc tgctgctcgc cgctgacgcg gcagccgcac
     7561 ctcccggcac gccggcgccg ctgctacccg ccgccgccgc agccgcagcc accgccggcg
     7621 gggatggcgg cgcacctggg cccccgccac ccactggtgg gcctggcggc agtaataaag
     7681 ccgggactcg ctgacgaggt gcgcctccca gaccacgacg accgccgcca ccgccaccac
     7741 cggtggtacc tggacgcgcg ggaaatctcc acctacatga gtgagccata gcggccgcac
     7801 ttcccccgga gcctccgctt ccgcccttgt acagctcgtc catgccgaga gtgatcccgg
     7861 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     7921 gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     7981 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     8041 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     8101 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     8161 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     8221 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     8281 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     8341 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     8401 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     8461 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     8521 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg
     8581 actgcagaat tcggttcact aaacgagccc ggttcactaa acgagctctg cttatataga
     8641 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     8701 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     8761 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     8821 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     8881 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     8941 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     9001 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     9061 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     9121 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     9181 attaataact agcacgcgtt cgaagcttga gctcggtccc ctcggggttg ggaggtgggt
     9241 ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa
     9301 aactattctt aaacctacca agcctcctac tatcattatg aataatttta tataccacag
     9361 ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc
     9421 ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt
     9481 gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag
     9541 agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga
     9601 gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct
     9661 tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat
     9721 agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac
     9781 cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa
     9841 caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt
     9901 gggtgctact cctaatggtt caatttttac tactttatat ttatataatt cacttctcca
     9961 attgtccctc atatctcctc cttatggtgt tttactaaac ttttccatgt tctaatcctc
    10021 atcctgtcta cttgccacac aatcatcacc tgccatctgt tttccataat ccctaatgat
    10081 ctttgctttt cttcttggca ctacttttat gtcactatta tcttgtatta ctactgcccc
    10141 ttcacctttc cagaggagct ttgctggtcc tttccaaagt ggatttctgc tgtccctgta
    10201 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
    10261 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
    10321 tttaaaattg tggatgaata ctgccatttg tactgctgtc ttaagatgtt cagcctgatc
    10381 tcttacctgt cctataattt tctttaattc tttattcata gattctacta ctccttgact
    10441 ttggggattg tagggaattt ccaggtctga agatcctgct gcttgctgtg cttttttctt
    10501 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
    10561 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
    10621 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
    10681 taacaggcca gtattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
    10741 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
    10801 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
    10861 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
    10921 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
    10981 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
    11041 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
    11101 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
    11161 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
    11221 ctccccagtc ccgcccaggc cacgcctccc atggtaatag cgatgactaa tacgtagatg
    11281 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
    11341 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
    11401 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
    11461 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
    11521 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
    11581 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtcg acccaggtgg
    11641 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
    11701 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
    11761 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
    11821 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
    11881 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
    11941 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
    12001 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
    12061 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
    12121 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
    12181 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
    12241 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
    12301 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
    12361 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
    12421 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
    12481 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
    12541 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
    12601 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    12661 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    12721 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    12781 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
    12841 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
    12901 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
    12961 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
    13021 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgg
//
