LOCUS       sequence-407532-       11367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, MEGF10_NUP210 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11367)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 11367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_MEGF10_NUP210
FEATURES             Location/Qualifiers
     source          1..11367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(493..1284,1285..1353))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1116..1135
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1354..1458)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        1489..1868
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     LTR             1870..2127
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2174..2299
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2791..2908
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    3268..3501
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3686..3730
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     enhancer        3987..4290
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4291..4494
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4445..4465
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      4570..4579
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(4576..4578,4579..4581,4582..5292)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4621..4642)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4882..4901)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5229..5250
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(9873..9892)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    9938..9993
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(9992..10011)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(10354..10370)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10354..10370)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10367..10389)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10378..10394
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10402..10408,10409..10426,10427..10432))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10447..10468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        10527..10722
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10573..10708
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    10728..10862
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10765..10784)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10819..10838
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10930..10947)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(join(11101..11367,1..322))
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(11181..11200)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
       61 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
      121 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
      181 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
      241 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
      301 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
      361 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
      421 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
      481 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
      541 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
      601 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
      661 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
      721 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
      781 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
      841 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
      901 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
      961 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     1021 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     1081 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     1141 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     1201 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     1261 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     1321 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     1381 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     1441 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgggtcga cattgattat
     1501 tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
     1561 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
     1621 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
     1681 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
     1741 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
     1801 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
     1861 ttaccatggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
     1921 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     1981 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     2041 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     2101 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     2161 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     2221 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     2281 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     2341 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     2401 ggagctagaa cgattcgcag ttaatactgg cctgttagaa acatcagaag gctgtagaca
     2461 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     2521 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     2581 agctttagac aagatagagg aagagcaaaa caaaagtaag aaaaaagcac agcaagcagc
     2641 aggatcttca gacctggaaa ttccctacaa tccccaaagt caaggagtag tagaatctat
     2701 gaataaagaa ttaaagaaaa ttataggaca ggtaagagat caggctgaac atcttaagac
     2761 agcagtacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     2821 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     2881 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gaaatccact
     2941 ttggaaagga ccagcaaagc tcctctggaa aggtgaaggg gcagtagtaa tacaagataa
     3001 tagtgacata aaagtagtgc caagaagaaa agcaaagatc attagggatt atggaaaaca
     3061 gatggcaggt gatgattgtg tggcaagtag acaggatgag gattagaaca tggaaaagtt
     3121 tagtaaaaca ccataaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     3181 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     3241 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     3301 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     3361 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     3421 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     3481 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     3541 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     3601 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     3661 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     3721 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     3781 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     3841 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     3901 accgagctca agcttcgaac gcgtgctagt tattaatagt aatcaattac ggggtcatta
     3961 gttcatagcc catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc
     4021 tgaccgccca acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg
     4081 ccaataggga ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg
     4141 gcagtacatc aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa
     4201 tggcccgcct ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac
     4261 atctacgtat tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg
     4321 cgtggatagc ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg
     4381 agtttgtttt ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca
     4441 ttgacgcaaa tgggcggtag gcgtgtacgg tgggaggtct atataagcag agctcgttta
     4501 gtgaaccggg ctcgtttagt gaaccgaatt ctgcagtcga cggtaccgcg ggcccgggat
     4561 ccaccggtcg ccaccatggt gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     4621 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag
     4681 ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     4741 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     4801 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     4861 gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga ggtgaagttc
     4921 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     4981 aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta tatcatggcc
     5041 gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat cgaggacggc
     5101 agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg ccccgtgctg
     5161 ctgcccgaca accactacct gagcacccag tccaagctga gcaaagaccc caacgagaag
     5221 cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct cggcatggac
     5281 gagctgtaca agggcggaag cggaggctcc gggggaagtg cggccgcttc ccggatggta
     5341 atctctctga actcctgctt atctttcatt tgtttgttgt tgtgtcactg gattgggacc
     5401 gctagtcctt taaatcttga ggacccaaac gtttgctctc attgggagtc ttatagtgta
     5461 acagtacagg aatcctatcc gcaccccttt gaccagattt attatacatc atgtactgat
     5521 atcttgaact ggttcaaatg tacgcgccac cgtgtatcct accgcacagc ctatcgtcat
     5581 ggagaaaaga ctatgtaccg tcgtaaatcg caatgttgtc caggctttta cgagtcagga
     5641 gaaatgtgcg taccgcactg tgcagataaa tgtgtgcatg gccgctgcat cgcacccaat
     5701 acgtgccagt gcgaacccgg ctgggggggg acgaattgtt ccagcgggcg tcttcctcca
     5761 ggttcagagc attgttcagg aatccgcgtc aaagcggagg cacagggctc aaccacttta
     5821 ttggtgtctt atcgtcacgg acatgtgcat ttgtcggcga aaatcacaat cgccgcctac
     5881 ttacctttaa aagctgtgga cccgagttcc gtcgctctgg taaccctggg aagttcgaag
     5941 gagatgttgt tcgaaggggg gccccgtcct tggattcttg agccgtcgaa gttctttcaa
     6001 aacgttacag cggaggacac cgatagcatt ggtctggcgt tatttgcgcc ccactcctct
     6061 cgcaattatc agcaacattg gattcttgta acgtgtcagg cacttggtga acaagtcatt
     6121 gctttgtccg tcggcaacaa accaagtctg acgaacccct ttcctgccgt tgagcctgca
     6181 gtagtaaaat ttgtatgtgc tcctccatct cgtctgacct tggctccagt atataccagt
     6241 cctcagttgg acatgtcgtg tcccctgttg cagcaaaaca aacaagtagt gccagtcagt
     6301 agccaccgta atcctcgcct ggatttagct gcctacgacc aagaaggtcg ccgtttcgac
     6361 aatttttcgt cccttagcat tcaatgggaa tcaacgcgtc cagttttggc ctctattgaa
     6421 ccggagttac ctatgcagct tgtgtctcag gacgacgagt ccggccagaa aaaactgcat
     6481 ggcctgcagg ccattctggt gcatgaggca agcggtacca ctgcaatcac tgctactgca
     6541 accggctatc aagaatctca tcttagttcg gcccgcacta agcagcccca tgatcccctg
     6601 gtgcccttat cggcgtcaat tgaactgatt ttagtcgaag acgttcgcgt gtcgccggag
     6661 gaggtgacta tttataatca ccctggtatc caggcagagt tgcgcattcg tgaggggtcc
     6721 ggttatttct ttcttaatac cagcactgcg gacgtggtaa aagtcgctta tcaggaggcc
     6781 cgtggcgtgg ccatggtaca cccactgctg cctggctcat cgacgatcat gattcacgac
     6841 ttatgtttgg tgtttcctgc accagctaag gctgtcgtgt atgtttcgga cattcaagag
     6901 ctgtatattc gcgtcgtaga caaggtcgaa attggcaaaa cggtgaaggc ctacgttcgc
     6961 gtgcttgact tgcataagaa acccttctta gcaaaatact tcccattcat ggatttaaaa
     7021 ttgcgcgcgg ctagccccat tatcacgtta gtagcgctgg atgaggcgtt ggataactac
     7081 acaatcacat ttttaattcg cggagtcgcg attggccaaa ccagtctgac agcttcagtc
     7141 acaaacaagg ccggtcaacg tattaattca gccccgcagc aaattgaggt tttccctcca
     7201 tttcgcctga tgccgcgtaa ggtgactttg ttaatcggcg caacaatgca ggttacatcc
     7261 gaaggtgggc ctcaaccgca atccaatatc ctttttagta tctcgaacga aagcgtggca
     7321 cttgtctcag ccgcgggact ggtacaaggg cttgctatcg gtaacggcac tgtaagtggc
     7381 cttgtccaag cggtagacgc cgaaacgggt aaggtggtga ttatttctca agatctggtt
     7441 caagtggaag tccttctttt gcgcgccgtg cgtattcgtg cgcccattat gcgcatgcgc
     7501 acggggacgc agatgccaat ctatgttacg ggtattacta accatcaaaa cccgttttct
     7561 tttggcaatg cggtcccggg tcttacgttc cactggtccg tgaccaaacg tgatgtgctg
     7621 gatttacgtg ggcgtcacca cgaagcatcc atccgcctgc cctctcagta caacttcgcg
     7681 atgaatgtat tggggcgtgt taagggccgc acgggtcttc gcgtcgtagt taaggccgta
     7741 gacccgacca gtgggcaatt atatggtctt gcccgcgaac tgtccgatga aattcaggta
     7801 caagtgtttg agaaattgca gcttctgaat ccagagattg aggcagaaca aattcttatg
     7861 tcaccgaact cgtacattaa acttcagacg aaccgcgacg gtgcggccag cttaagctat
     7921 cgcgtccttg atggccctga aaaggttcca gtcgtgcatg tagatgaaaa ggggttctta
     7981 gcatcaggtt ctatgattgg cactagtacc attgaagtta ttgctcaaga accctttggt
     8041 gcgaaccaga ctatcattgt ggcggtaaag gtttcgcctg taagttacct gcgcgtgtcc
     8101 atgtcaccgg ttttgcatac acaaaataag gaagcgttgg tcgcggttcc gcttgggatg
     8161 acggtaacat tcactgtaca ttttcacgat aattccgggg atgtgttcca tgctcatagt
     8221 agtgtgctga acttcgccac taatcgtgat gactttgttc aaatcggtaa aggtccaaca
     8281 aacaacacct gtgtcgtgcg cacggtttca gttggcttga cgttgctgcg tgtctgggat
     8341 gcggaacacc caggtttgag tgacttcatg cctcttcctg ttttgcaggc gatcagtcca
     8401 gaactgtcag gcgctatggt cgtgggtgat gtcctttgct tagcaactgt cttgaccagc
     8461 ttggaggggc tttcgggtac atggtcttca agcgcgaact caattcttca catcgacccg
     8521 aagactggtg tggctgtcgc acgcgcagta ggatcagtta cggtatatta tgaggtggca
     8581 ggacatttac gcacctacaa ggaagtagtc gtctccgtac cgcagcgcat tatggctcgt
     8641 cacttgcacc ccatccaaac aagttttcaa gaggccactg cgtcaaaggt gattgtagcc
     8701 gtaggtgatc gtagctctaa tctgcgcggg gaatgtacac ctactcaacg cgaggttatc
     8761 caagcgttgc atccggaaac gcttatctca tgtcagtctc agttcaaacc agctgtcttt
     8821 gatttcccta gtcaagacgt ctttacggtt gaaccacaat ttgacacagc tctggggcaa
     8881 tatttctgct ccatcaccat gcatcgttta acggacaagc agcgcaaaca cctttcaatg
     8941 aagaaaacgg ccctggttgt ttcggcgagc ttaagttctt cccacttttc gacggagcaa
     9001 gtaggtgcag aagtgccatt ctcacccgga ctgttcgcag accaggcgga aattctttta
     9061 tctaatcact acacatcttc tgagatccgc gtatttggcg caccggaagt cttagaaaac
     9121 ttggaagtta aatccggttc ccccgctgtc ctggcgtttg ccaaggaaaa gagctttgga
     9181 tggccctctt tcatcactta cacagtcgga gttctggacc ctgctgcagg ttctcaaggt
     9241 ccacttagca caaccttgac attctctagc cccgtcacca atcaagcaat tgccattcct
     9301 gtcacagttg cgtttgtagt tgaccgccgc ggacccggtc cgtacggcgc ttctcttttt
     9361 caacattttt tggactctta tcaggtaatg ttttttacgc tgttcgcttt gcttgcaggc
     9421 acggctgtga tgattatcgc gtatcacact gtctgtacgc cgcgtgattt ggcagtgcca
     9481 gctgcgctga ccccgcgtgc ctcgcctggc catagccctc actacttcgc tgcttcatcc
     9541 ccaacctcac ctaatgctct tcctccagcg cgcaaggcat ccccgccttc cggcttatgg
     9601 tcacccgcat acgcgagtca ttgaaccggt tctagataag attacggtac ctttaagacc
     9661 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     9721 agggctaatt cactcccaaa gaagacaaga tctgcttttt gcctgtactg ggtctctctg
     9781 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     9841 tcaataaagc ttcagctgct cgagctagca gatctttttc cctctgccaa aaattatggg
     9901 gacatcatga agccccttga gcatctgact tctggctaat aaaggaaatt tattttcatt
     9961 gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg gagggcaaat
    10021 catttaaaac atcagaatga gtatttggtt tagagtttgg caacatatgc ccatatgctg
    10081 gctgccatga acaaaggttg gctataaaga ggtcatcagt atatgaaaca gccccctgct
    10141 gtccattcct tattccatag aaaagccttg acttgaggtt agattttttt tatattttgt
    10201 tttgtgttat ttttttcttt aacatcccta aaattttcct tacatgtttt actagccaga
    10261 tttttcctcc tctcctgact actcccagtc atagctgtcc ctcttctctt atggagatcc
    10321 ctcgacctgc agcccaagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
    10381 ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg
    10441 tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc
    10501 gggaaacctg tcgtgccagc ggatccgcat ctcaattagt cagcaaccat agtcccgccc
    10561 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
    10621 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
    10681 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctaac ttgtttattg
    10741 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
    10801 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
    10861 tccgctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc
    10921 ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
    10981 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
    11041 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
    11101 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
    11161 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
    11221 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
    11281 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
    11341 caagctgggc tgtgtgcacg aaccccc
//
