LOCUS       sequence-407535-       13941 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, KMT2A_EPS15 from 
            patient-derived sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13941)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 13941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_KMT2A_EPS15
FEATURES             Location/Qualifiers
     source          1..13941
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(join(8702..9412,9413..9415,9416..9418))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(8744..8765)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     9093..9112
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9352..9373
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      9415..9424
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(9500..9703)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9529..9549)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9704..10007
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(10264..10308)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10493..10726
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    11086..11203
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    11695..11820
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             11867..12124
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        12126..12505
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        12536..12640
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(12641..12709,12710..13501)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(12859..12878)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(13672..13941,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagac taagcctcgg
     1801 agatctccga cttgcttaac gcgatggcca gctccagatc ctcttgttcc tgctggttta
     1861 aacgtgctaa gcgttgctct tcttcgcgct cggattcacg ttttgcccac tcaatcatgt
     1921 cctcctctga tggataggca gaaaaatttg cgaagttgga cgggtccgct tctttattgg
     1981 tggtggtggt cgcgctggta aacggatcac agaagatctc tgggtccttt tctttcggag
     2041 agtcgttccc cggaaacggc tggaacgggt cgtttaattt gaacggatcg ggtgagtcta
     2101 acttattaat gctacgttta cccggcggca acggacaagg acgggtcggc gtgccgattt
     2161 tcggcggcag cgctgggggc tcgtcctcgc tctttacaga tgtttcttca aacacgtttt
     2221 tggttatcac aacgttggag acggaggaac ttgtggcgct gcgaaagggg tcttcgttgt
     2281 taaccttgct cagagtagag aagtcggcga aaccaccacc aaaactctca ttgccgaaca
     2341 cactcgcgaa cggatcggtc gcgctgtcag aggccgcaac aacggtaccg cccggagcga
     2401 acggatcatt gtgcttcaga gtttctacgg aggtgataga ggagttgttc gcagcgctaa
     2461 acgggtcggt gctgctggtt gcgaacgggt cggtactctg acggaaaaaa cagtcggagg
     2521 cgaacgggtc actgcccttg aacggatcgc cgccgaacgg atcaattttc ccgaacgggt
     2581 cgtctttaaa agggtccgag cccacgaacg ggtcgctctg gaaaaaatcc aggttagtgt
     2641 ccgccaccgg cccggtaagg gagctactat caacgttgaa agggtcctcc tccttgctat
     2701 gacggttgtt gtccaattca ctgcacactt tttcggtaac ggcggtcgta acttcattct
     2761 cgtccgtaac accggacggc agcagctccg gagagctacg cgctgggctc tcctgatgaa
     2821 tcggctcaga ctccaagttg ctctgtccct caacatgttc gttgaggttg gcggtctcgc
     2881 tagaggaggt actcagggag cagtaatcgg tggcaccatt aaccaggata ctatgggggc
     2941 tactacacca gtttagttgg ctgttatggt tttccaaatc tttcatttcc attaacttca
     3001 tttgcattga ggagatttcc tgctggctgt cttgcaggtg ttgctgcagc ggctcaagtt
     3061 gggctttacc cgattcgaca gattcttcca gttcggcggt ctcctgttgc aggcgactca
     3121 actcttcgcg cgcctttgcc agctcctctt cataggtgct gatctgggat tcctgggagg
     3181 ttaattctgc tttcaaagac gagataagct gcgcttcctc tgcgcacttt ttacgaacct
     3241 ctttcagctg ttcctctaat tgcgctttct gctcatctaa ttcatccagc agttcttgca
     3301 cttgttgttt ttgcgcttgc agtttttgta agttagtatt ttcacgctgc acttcatcct
     3361 gcaggtcctg aacctcgctt gtacgttgct taatggtgtc ctccttttcc ttcagatctt
     3421 gttccacatt gttcttttca cgctgcagat caactatttc attattgagg gtgtcaagct
     3481 ctttgatagc agagaagtcg gcaaccggtg agctaccgat gatgtttttt tgcaggctcg
     3541 cacggtcact cggcggaatc atctccgggg tcagcacgtg cggcggatcg atccctttaa
     3601 tcagcttctg cgaaatcagg tgaaacgcga gagcgaactg gtccttggac aacttaccgc
     3661 aatctttggt gtcgcaaagt gaccaaatgt gcgccaacag cgtgctcggc agacctgttt
     3721 ttagaaaaat ctctctgacc tccagcccgg acacaaaacc atccatgtct ttgtccgttt
     3781 tgaggaaaat ctcatcgtac ttcgcttttt ctgccgggga gacaacccag gttttgcgtt
     3841 tgctaggcgg cacaagcgct ggcggcaggc tcatcggaac cggttctttt tccaacgcgc
     3901 aataaaccag gaacattgca actgcgaact cgtcacggtc cagcatacca tcgtggtcga
     3961 tgtcgctcag ttcccataca cggcccaaga tgtcaactgg cagcttgctg ttcagcagaa
     4021 caggttttac cttatcacca gacagaaaac cgttcaccgg gctaaggctg tcgaagatcg
     4081 catcgtattt cgctttgtcc tcgggtttga ctgcccacgg gagttccgcc gctgacgtac
     4141 ctgaaatcag cagtggggag gaggtatcgt ggaatctcgg cggtggcacc gccaggttga
     4201 ggctgctcag acttacctcc aaaccattct gagcgcacgc caccaggcgc agtgccacga
     4261 agaactcctg tttattcaga ataccttttc cgtcggtatc agccagatcc caaatcttac
     4321 caagaatgag atccggcagg ccgcttttct tcagaaacgc agcagcgtcg gatgcaagta
     4381 ctctgccagt gttgccggta tcaacctgtc tatagtattt ttcgtaaact ggattgccac
     4441 tactgagttt gaaatccaca cgaatacgat gaacaccgtc cgccggaatc ttttgcttgc
     4501 tgctgttacc gttactcagg gtggacaaaa tattcaaggt gcctgcgttt tcttgtttgt
     4561 ttacgggcgg aggcttttcc ttctctttgg gtttttgctt caccggaata gacggacgcg
     4621 gtgccacctt tttctgcttc gactgctccg gaccgctttc cggtggcggc ggctgttttt
     4681 ttttcggctc gctaggggtg gtcttcggca cctctttgcg cggcggacct gtggtcggcg
     4741 gctgcggcgg aatgaccaag gccggttgtg aaacctgttt ggaagattta cgactagctg
     4801 gagtcgtggc ctgcttggac tccgggcccg gtgcgctaac attaccttct tcacttttct
     4861 cttccaccgg tttccgcggc ggaggctcag agctgctctt ttttggtgcc gggtcttcac
     4921 gagcgctcgg cgtgggcttc tgggagctat ctaccacatt cttgacgacg ctcgactcct
     4981 tgctgtcctt tttctcagaa gtcttggatt ttttctcttt tttcttaact gccttggcct
     5041 gtttctgcag gtacgccttg gatggcatcc attgcaagtt ctggcattta cgcattttac
     5101 agcattgctt tttaatgtta cgaccgccga acttgggttt atccagacaa ttggtgcaga
     5161 cgccgcaatc ctccgggacc tgacaacccg ggcactgacc acaacgacga gaacggcggc
     5221 ccttcttaac aggcggctct tgcggcgctt tattacgggt caccggttta atcggcttaa
     5281 tcggcggagc cagcggctca gcatcctcgc tacccgcgat cgatgactta tcatcgttac
     5341 ccatgctgct cagaatcttc tcgcgttctt cccacggcag tgcgctcagc gtcggcatgt
     5401 cgtctgggaa aactgcgcgc ttacggccca gcgcaacggc agcacgacgg caaacgtgtt
     5461 taatgcgcgg acctctcacc gatgtctcac tactatcact ttcttgacct tgagcttttg
     5521 gctgatccgt ttgtttgagc gacttcgatt tttcaatttt gcacagctga gcctttgcct
     5581 tcttgagcag gctcgcaaca cgcttatcgg tcataggcaa tttgtccgct tgagccagca
     5641 tagagccgat cgaggaggtg gagtgcttca cggtagagga gctcggcgtg ctcaggcaca
     5701 aggttttctc cttctccaga ctcggagccg tcgggcccaa atccaggttg gtcttctcca
     5761 agttgccacg gccctttttg attaagattt tggtcttcac cgcagtcgta tcacccaggg
     5821 tgacgctggt gatatccgtt ccggagtcgt gagagctcga tttcttacga ccggtcgcct
     5881 tcttggcgga agaggaggta gcaacgtctt caccaaccac tttctcttta gacacacggc
     5941 caaccggata cagcgcagag ctgctctgga tttcgctacc tttctttcgt ttttctttac
     6001 gagattccct cttgttctct ttttcgcgct cacgatcacg ctcacggctc ttgtcttttt
     6061 ccacgctctt gtcagcatcc cggtccttgg atagctcctc cggcgctttg tctttgttac
     6121 gaccgcgctc ggtctgactg cccggggtaa accacgggaa cagcggcgtt ggggaggagg
     6181 agctaaacgg ttcagctggc gcgctcgtct gcttacgcgg acgctggttc ttctccgcgc
     6241 tttcgccact ctgcgtcagc gagtgagacg gaaaggtgaa ggtcgggttc aatgcactgg
     6301 tcgcgagcgg gctcacagag atactgaggc tactgctcac ggaggaaggc ggggtcagag
     6361 gtgacagttc actggagctc aggcgaccgc tacgcgttct catggagtgc gacgggctac
     6421 gaggctccga acgaatcggg ctgaacactt ttcgctttct cttacgatta gacacaccag
     6481 agctgctagt acctgcgctg gtacggttag atgggagggt tacgctttca aagatacggc
     6541 tgtgtgcctc agacggggtg aagcgcgggg cacgtaacag cggtgaacgc ttatgcatat
     6601 cgaaacgggt cccggaatgc agcggactaa agagacgggc agacgctgca gtgcctgatg
     6661 cagaaaagcc ggacgcgaag cccacgtctt ccggagtcaa tggcggcgga cggaaattat
     6721 caaagatagg tttgcgtatg agaccctcct tagcgtattt cgccgagcta aaatattgcg
     6781 gctcgctccg actgtgtttg agagaggtcc agcggaaggt cggttcgcgc aggatagact
     6841 tgcgcttgcc ctgcataggg gcagttgatg ccgggagaaa cggagaagcc agcggaatgg
     6901 tcggaggcat cagccacggg gtgtggtcgg agattgaaga ggccggttgc agcggcggcg
     6961 gcggagtcag caagggcgga ggcggggaag agctggtttg ttgctgcggc gcactttgaa
     7021 gggtgctcaa ttttttggtc gtgcgggaac caaagctgcg ttctgaaacg ctataacggc
     7081 gactacgtct atcgttggat tcattttccg gggattggct gatcggcaac ggcggatgca
     7141 cttccggtgt atcgctacgt tcttccggca gcacttggat ttcctcgctc gcttgggagt
     7201 cggtagacgt atccacggat gggctggaag aacgagatga gtcgctgctc atttgcgagc
     7261 tatgctggct cgcagctgag cttttttcgc tggaaccgca agatggcgcc gaaaagcggc
     7321 tgttcggtgt agattccagg cgggcaatct taatcggcgg gtcgtagtct tcgtcttcga
     7381 taaaacggcg cggggttttg ataatgcggc tgctaatcgc tgaaacgacc ggcataatga
     7441 actgacgaat gttcttaact tgggttttca cctttctgcc ctgcagttgg gctgcttcct
     7501 tctcgatctt tttctgtgct ccttttttcg cacgttgcaa caactgcttc gcaatggtcg
     7561 cgtcggtacg cttcgacgag ggaattatac gaaccggttt gatgcgacgc gggctctgac
     7621 gcaccacggt tttatcctcc ttggtcagcg gaggagtgcc ctctttgtcc ttacgaacct
     7681 tttgtggttt ctccagctcg ctattgatca gcagacctga cggagtctta atacgttcgg
     7741 tgctaggcgg cctccctctg cgacgcacga tttgaacacc cttacggccg atctgcagct
     7801 tacccgtttt gaactttgac ttcagtggcg acagcttacc tgcacgtagc ttcttaattt
     7861 tggtcgcctg ttgaaacgtt gcactcggag ttctcttgat ttttttcagt gaatcttctt
     7921 tgttgccttt cggcagttcg ctgatatcct taccatgcgt gatcttaatt ttgacgcccg
     7981 gaaacgtcgg tggacggcca cgcttttttt caatggattt agagtccttc tttttgatct
     8041 tatctccgct tttggtctcg cttttgttca gaggcgaaaa cactgacggg tcgctcagga
     8101 tcgcgctatt acggtcggag ccggaccgag gacggccacg cggctttctc gggctcgttt
     8161 tcacggaggg gctcctggtc ggtgagcgaa cacggacctc ttcgtctgaa ccaaaaccca
     8221 gaaactgctc gtcttcaccg gagccaccgc cgccgccgga ctcgccaaac acggctcgga
     8281 agcgacgtaa gttcgtaccg atcgccgcgc taacttgcag tgcggcgtcg aatcccgggc
     8341 ctacgcgcag cagagctgga ccggagctcg ccgaggagga gctgctgcta ctactgctcg
     8401 cagaggagga agaggcggca gacgccgccg ctgcaccacc cggaacacct gcgccggagc
     8461 tacccgccgc cgctgccgca gcggccactg ccggcggcga tggcggcgcg ccagggccac
     8521 ccccgcctac aggcggacca ggcggcagca acagagccgg cacgcgctgc cgcggggcac
     8581 cgcccagacc acggcgtcca ccgcctccgc cgccgccggt ggtgcctggt ctcgcgggaa
     8641 acctccatct acatgagtga gccatagcgg ccgcacttcc cccggagcct ccgcttccgc
     8701 ccttgtacag ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga
     8761 ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt ggactgggtg ctcaggtagt
     8821 ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg
     8881 cgagctgcac gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt
     8941 tcttctgctt gtcggccatg atatagacgt tgtggctgtt gtagttgtac tccagcttgt
     9001 gccccaggat gttgccgtcc tccttgaagt cgatgccctt cagctcgatg cggttcacca
     9061 gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta gttgccgtcg tccttgaaga
     9121 agatggtgcg ctcctggacg tagccttcgg gcatggcgga cttgaagaag tcgtgctgct
     9181 tcatgtggtc ggggtagcgg ctgaagcact gcacgccgta ggtcagggtg gtcacgaggg
     9241 tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa cttcagggtc agcttgccgt
     9301 aggtggcatc gccctcgccc tcgccggaca cgctgaactt gtggccgttt acgtcgccgt
     9361 ccagctcgac caggatgggc accaccccgg tgaacagctc ctcgcccttg ctcaccatgg
     9421 tggcgaccgg tggatcccgg gcccgcggta ccgtcgactg cagaattcgg ttcactaaac
     9481 gagcccggtt cactaaacga gctctgctta tatagacctc ccaccgtaca cgcctaccgc
     9541 ccatttgcgt caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt
     9601 gccaaaacaa actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa
     9661 accgctatcc acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga
     9721 ctaatacgta gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat
     9781 gccaggcggg ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca
     9841 cttgatgtac tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga
     9901 aagtccctat tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt
     9961 cgttgggcgg tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat
    10021 atgggctatg aactaatgac cccgtaattg attactatta ataactagca cgcgttcgaa
    10081 gcttgagctc ggtcccctcg gggttgggag gtgggtctga aacgataatg gtgaatatcc
    10141 ctgcctaact ctattcacta tagaaagtac agcaaaaact attcttaaac ctaccaagcc
    10201 tcctactatc attatgaata attttatata ccacagccaa tttgttatgt taaaccaatt
    10261 ccacaaactt gcccatttat ctaattccaa taattcttgt tcattctttt cttgctggtt
    10321 ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa ttgttaattt ctctgtccca
    10381 ctccatccag gtcgtgtgat tccaaatctg ttccagagat ttattactcc aactagcatt
    10441 ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa ccccaaatcc ccaggagctg
    10501 ttgatccttt aggtatcttt ccacagccag gattcttgcc tggagctgct tgatgcccca
    10561 gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc ctcagcaaat tgttctgctg
    10621 ctgcactata ccagacaata attgtctggc ctgtaccgtc agcgtcattg acgctgcgcc
    10681 catagtgctt cctgctgctc ccaagaaccc aaggaacaaa gctcctattc ccactgctct
    10741 tttttctctc tgcaccactc ttctctttgc cttggtgggt gctactccta atggttcaat
    10801 ttttactact ttatatttat ataattcact tctccaattg tccctcatat ctcctcctta
    10861 tggtgtttta ctaaactttt ccatgttcta atcctcatcc tgtctacttg ccacacaatc
    10921 atcacctgcc atctgttttc cataatccct aatgatcttt gcttttcttc ttggcactac
    10981 ttttatgtca ctattatctt gtattactac tgccccttca cctttccaga ggagctttgc
    11041 tggtcctttc caaagtggat ttctgctgtc cctgtaataa acccgaaaat tttgaatttt
    11101 tgtaatttgt ttttgtaatt ctttagtttg tatgtctgtt gctattatgt ctactattct
    11161 ttcccctgca ctgtaccccc caatcccccc ttttctttta aaattgtgga tgaatactgc
    11221 catttgtact gctgtcttaa gatgttcagc ctgatctctt acctgtccta taattttctt
    11281 taattcttta ttcatagatt ctactactcc ttgactttgg ggattgtagg gaatttccag
    11341 gtctgaagat cctgctgctt gctgtgcttt tttcttactt ttgttttgct cttcctctat
    11401 cttgtctaaa gcttccttgg tgtcttttat ctctatcctt tgatgcacac aatagagggt
    11461 tgctactgta ttatataatg atctaagttc ttctgatcct gtctgaaggg atggttgtag
    11521 ctgtcccagt atttgtctac agccttctga tgtttctaac aggccagtat taactgcgaa
    11581 tcgttctagc tccctgcttg cccatactat atgttttaat ttatattttt tctttccccc
    11641 tggccttaac cgaatttttt cccatcgcga tctaattctc ccccgcttaa tactgacgct
    11701 ctcgcaccca tctctctcct tctagcctcc gctagtcaaa atttttggcg tactcaccag
    11761 tcgccgcccc tcgcctcttg ccgtgcgcgc ttcagcaagc cgagtcctgc gtcgagagag
    11821 ctcctctggt ttccctttcg ctttcaagtc cctgttcggg cgccactgct agagattttc
    11881 cacactgact aaaagggtct gagggatctc tagttaccag agtcacacaa cagacgggca
    11941 cacactactt gaagcactca aggcaagctt tattgaggct taagcagtgg gttccctagt
    12001 tagccagaga gctcccaggc tcagatctgg tctaaccaga gagacccagt acaggcaaaa
    12061 agcagctgct tatatgcagg atctgagggc tcgccactcc ccagtcccgc ccaggccacg
    12121 cctcccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
    12181 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
    12241 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
    12301 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
    12361 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
    12421 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
    12481 taataactag tcaataatca atgtcgaccc aggtggcact tttcggggaa atgtgcgcgg
    12541 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
    12601 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
    12661 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
    12721 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
    12781 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
    12841 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
    12901 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
    12961 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
    13021 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
    13081 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
    13141 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
    13201 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
    13261 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
    13321 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
    13381 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
    13441 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
    13501 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
    13561 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
    13621 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
    13681 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
    13741 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
    13801 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
    13861 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
    13921 cgataagtcg tgtcttaccg g
//
