LOCUS       sequence-407537-        8865 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Express mEGFP-tagged fusion protein, MYC_MYC from patient-derived 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8865)
  AUTHORS   Tripathi S, Shirnekhi HK, Gorman SD, Chandra B, Baggett DW, Park CG,
            Somjee R, Lang B, Hosseini SMH, Pioso BJ, Li Y, Iacobucci I, Gao Q, 
            Edmonson MN, Rice SV, Zhou X, Bollinger J, Mitrea DM, White MR, 
            McGrail DJ, Jarosz DF, Yi SS, Babu MM, Mullighan CG, Zhang J, Sahni 
            N, Kriwacki RW
  TITLE     Defining the condensate landscape of fusion oncoproteins.
  JOURNAL   Nat Commun. 2023 Sep 28;14(1):6008. doi: 10.1038/s41467-023-41655-2.
  PUBMED    37770423
REFERENCE   2  (bases 1 to 8865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-09-28"; volume: "14(1):6008. doi"; pages: " 
            10.1038/s41467-023-41655-2"
COMMENT     Sequence Label: CL20_mEGFP_MYC_MYC
FEATURES             Location/Qualifiers
     source          1..8865
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..239
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     473..490
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    558..692
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(582..601)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     636..655
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(698..893)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      712..847
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    952..973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(988..993,994..1011,1012..1018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1026..1042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1031..1053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1050..1066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1050..1066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1409..1428
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    1427..1482
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     1528..1547
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(1859..1888)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(2132..2158)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(2282..2311)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(2555..2581)
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             complement(join(3626..4336,4337..4339,4340..4342))
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3668..3689)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     4017..4036
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4276..4297
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      4339..4348
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(4424..4627)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(4453..4473)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        4628..4931
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     CDS             complement(5188..5232)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5417..5650
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    6010..6127
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     misc_feature    6619..6744
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             6791..7048
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     enhancer        7050..7429
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7460..7564
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7565..7633,7634..8425)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7783..7802)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8596..8865,1..319)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
       61 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
      121 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
      181 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
      241 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
      301 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
      361 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
      421 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
      481 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
      541 gattcattaa tgcagcggat ccagacatga taagatacat tgatgagttt ggacaaacca
      601 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
      661 ttgtaaccat tataagctgc aataaacaag ttagcttttt gcaaaagcct aggcctccaa
      721 aaaagcctcc tcactacttc tggaatagct cagaggccga ggcggcctcg gcctctgcat
      781 aaataaaaaa aattagtcag ccatggggcg gagaatgggc ggaactgggc ggagttaggg
      841 gcgggatggg cggagttagg ggcgggacta tggttgctga ctaattgaga tgcggatccg
      901 ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag
      961 ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg
     1021 tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa
     1081 gcttgggctg caggtcgagg gatctccata agagaagagg gacagctatg actgggagta
     1141 gtcaggagag gaggaaaaat ctggctagta aaacatgtaa ggaaaatttt agggatgtta
     1201 aagaaaaaaa taacacaaaa caaaatataa aaaaaatcta acctcaagtc aaggcttttc
     1261 tatggaataa ggaatggaca gcagggggct gtttcatata ctgatgacct ctttatagcc
     1321 aacctttgtt catggcagcc agcatatggg catatgttgc caaactctaa accaaatact
     1381 cattctgatg ttttaaatga tttgccctcc catatgtcct tccgagtgag agacacaaaa
     1441 aattccaaca cactattgca atgaaaataa atttccttta ttagccagaa gtcagatgct
     1501 caaggggctt catgatgtcc ccataatttt tggcagaggg aaaaagatct gctagctcga
     1561 gcagctgaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     1621 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagat cttgtcttct
     1681 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     1741 agctgccttg taagtcattg gtcttaaagg taccgtaatc ttatctagaa ccggttcacg
     1801 cacaactgtt gcgcaattgt tctaacttgt gcttcaattg ctcacggcgc ttgcgcaaca
     1861 aatcttcttc actgataagt ttttgctctt ccgcctgtac cgataaaata tatgctgtcg
     1921 cttttttcaa aattaccacc ttgggggcct tttcgttatt ctccagttcg ggaatttggt
     1981 cacgcagtgc aaaaaatgaa cgttttaatt cattacggcg ttggcgttcc aacacgttgt
     2041 gagtacgacg ttttacgttc tcctccgtgt ctgatgagcg aggactggta catttgcgat
     2101 tgttgctgat ttgacgaaga acacgaactg aatccagctt aacacgtttg gccgctggat
     2161 aatctttgcg tgtgctgggg ggagcggcat aattgtgctg gtgcgtactc acgtggcaac
     2221 gtttcagtac caaggggaac tgttccagtt tgtgtttcag ttgctcgcga cgcttacgta
     2281 ataagtcttc ttcgctgata agtttttgtt cttcagcctg gacggacaga atgtatgcgg
     2341 tcgctttctt taagatgacg actttcggag ccttttcatt attttctaat tcaggaatct
     2401 ggtcgcgtaa agcaaaaaag gaacgcttaa gttcgttgcg acgctgacgt tctaagacgt
     2461 tatgcgtacg gcgcttgacg ttttcttcag tatcagacga acgcggcgat gtgcacttgc
     2521 ggttgtttga gatttgacgc agaacgcgga cggaatccag cttcacacgc ttggcagccg
     2581 ggtaatcctt gcgagttgag ggcggcgcgg catagttgtg ttgatgagtg ctaacatgac
     2641 aacgtttcaa aaccaatggc gaatggggag gtttcgaatg tcccccggca gatggggaac
     2701 ctgattctga acgttttccg ggggcttgac gcttttcgac cgagacgacg tcgatctcct
     2761 cctcatcttc ttgttcctcc tcagagtcgg atgatgtcgt gggcggagtc tcttcgtgca
     2821 gcacaagcgg ttcgggagac ccttgagggg acgattcagt tgacgataac aagctatcgc
     2881 tagacggcga gaaagcagaa gaatcctggc ttgcacaaga tttggggcta ctggagtcgt
     2941 tcaaagggta cgggaaaact accgaaggat cgatgcattc actggccgca gcggaaaggt
     3001 cttgtaaata taagctgctc gtcgaacaga cagaatgacc gcgagccggg ttcggggacc
     3061 ctgagtcctt acgggctgct tgatatgatg ccaatttttc gctaactaac ttggcggctg
     3121 cgctaaagcc gctccacata cagtcttgga tgatgatgtt tttgataaat gtctcgtcat
     3181 cgggatcaca gatgaatgac tggttcacca tgtcaccccc cagcagttcc gtgaccatct
     3241 caagttgatc agccgttgag aaagaacctc ccccaccgtc gttgtcacca cgtaaagaaa
     3301 agggagtgac cgctacatag ctcgggctgc acagtcccga gcggcgactg gggctcagtg
     3361 gtggcgtggg aagcaactca aactttttcc aaatatcttc actcggcgca ggaggctgta
     3421 attctgactg ctgctgctgt tgataaaaat tctcctcctc atcacaatag aaataaggtt
     3481 ggaccgaatc ataatccaaa tcatagttgc ggttagtaaa agagacgttt aagggcattg
     3541 tggccggcgg ctgattctct acaacacgaa agaagtccat tccccgggaa gcggccgcac
     3601 ttcccccgga gcctccgctt ccgcccttgt acagctcgtc catgccgaga gtgatcccgg
     3661 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     3721 gcttggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     3781 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     3841 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     3901 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     3961 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     4021 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     4081 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     4141 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     4201 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     4261 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     4321 gctcctcgcc cttgctcacc atggtggcga ccggtggatc ccgggcccgc ggtaccgtcg
     4381 actgcagaat tcggttcact aaacgagccc ggttcactaa acgagctctg cttatataga
     4441 cctcccaccg tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt
     4501 ttggaaagtc ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga
     4561 gacttggaaa tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc
     4621 gcatcaccat ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc
     4681 ataaggtcat gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag
     4741 ggggcgtact tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat
     4801 actccaccca ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca
     4861 ttattgacgt caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa
     4921 gttatgtaac gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact
     4981 attaataact agcacgcgtt cgaagcttga gctcggtccc ctcggggttg ggaggtgggt
     5041 ctgaaacgat aatggtgaat atccctgcct aactctattc actatagaaa gtacagcaaa
     5101 aactattctt aaacctacca agcctcctac tatcattatg aataatttta tataccacag
     5161 ccaatttgtt atgttaaacc aattccacaa acttgcccat ttatctaatt ccaataattc
     5221 ttgttcattc ttttcttgct ggttttgcga ttcttcaatt aaggagtgta ttaagcttgt
     5281 gtaattgtta atttctctgt cccactccat ccaggtcgtg tgattccaaa tctgttccag
     5341 agatttatta ctccaactag cattccaagg cacagcagtg gtgcaaatga gttttccaga
     5401 gcaaccccaa atccccagga gctgttgatc ctttaggtat ctttccacag ccaggattct
     5461 tgcctggagc tgcttgatgc cccagactgt gagttgcaac agatgctgtt gcgcctcaat
     5521 agccctcagc aaattgttct gctgctgcac tataccagac aataattgtc tggcctgtac
     5581 cgtcagcgtc attgacgctg cgcccatagt gcttcctgct gctcccaaga acccaaggaa
     5641 caaagctcct attcccactg ctcttttttc tctctgcacc actcttctct ttgccttggt
     5701 gggtgctact cctaatggtt caatttttac tactttatat ttatataatt cacttctcca
     5761 attgtccctc atatctcctc cttatggtgt tttactaaac ttttccatgt tctaatcctc
     5821 atcctgtcta cttgccacac aatcatcacc tgccatctgt tttccataat ccctaatgat
     5881 ctttgctttt cttcttggca ctacttttat gtcactatta tcttgtatta ctactgcccc
     5941 ttcacctttc cagaggagct ttgctggtcc tttccaaagt ggatttctgc tgtccctgta
     6001 ataaacccga aaattttgaa tttttgtaat ttgtttttgt aattctttag tttgtatgtc
     6061 tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc ccccttttct
     6121 tttaaaattg tggatgaata ctgccatttg tactgctgtc ttaagatgtt cagcctgatc
     6181 tcttacctgt cctataattt tctttaattc tttattcata gattctacta ctccttgact
     6241 ttggggattg tagggaattt ccaggtctga agatcctgct gcttgctgtg cttttttctt
     6301 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
     6361 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
     6421 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
     6481 taacaggcca gtattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
     6541 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     6601 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
     6661 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
     6721 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
     6781 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     6841 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     6901 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     6961 cagagagacc cagtacaggc aaaaagcagc tgcttatatg caggatctga gggctcgcca
     7021 ctccccagtc ccgcccaggc cacgcctccc atggtaatag cgatgactaa tacgtagatg
     7081 tactgccaag taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat
     7141 ttaccgtcat tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc
     7201 aagtgggcag tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc
     7261 gttactatgg gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag
     7321 ccaggcgggc catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact
     7381 aatgaccccg taattgatta ctattaataa ctagtcaata atcaatgtcg acccaggtgg
     7441 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     7501 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     7561 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     7621 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     7681 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     7741 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     7801 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     7861 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     7921 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     7981 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     8041 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     8101 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     8161 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     8221 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     8281 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     8341 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     8401 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
     8461 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
     8521 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
     8581 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa
     8641 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc
     8701 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta
     8761 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct
     8821 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgg
//
