LOCUS       sequence-407588-        9874 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Female specific eGFP with CctraF, dsRed in both sexes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9874)
  AUTHORS   Liu J, Rayes D, Akbari OS
  TITLE     A fluorescent sex-sorting technique for insects with the 
            demonstration in Drosophila melanogaster.
  JOURNAL   bioRxiv. 2023 Aug 14:2023.08.11.553026. doi: 
            10.1101/2023.08.11.553026. Preprint.
  PUBMED    37645836
REFERENCE   2  (bases 1 to 9874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Aug 14:2023.08.11.553026. doi: 10.1101/2023.08.11.553026. 
            Preprint."
COMMENT     Sequence Label: 795L
FEATURES             Location/Qualifiers
     source          1..9874
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   624..686
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1004..1389
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             complement(join(2064..2738,2739..2741,2742..2744))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(2126..2149)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2324..2343)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2541..2561
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     protein_bind    3304..3689
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     enhancer        3696..4178
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        4182..4773
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             6134..6847
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             6134..6844
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(6173..6194)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6434..6453)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     6781..6802
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    6957..7078
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6994..7013)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7048..7067
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    7106..7175
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     repeat_region   7645..7679
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(7686..7704)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7725..7741)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7725..7741)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7738..7760)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7749..7765
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7773..7779,7780..7797,7798..7803))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7818..7839
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7956..7973)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8127..8715)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8207..8226)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8886..9677,9678..9746))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9509..9528
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9747..9851)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtagat taaccctaga aagataatca tattgtgacg tacgttaaag
      661 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      721 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      781 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaactttc gttgatagaa
      841 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      901 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      961 tttttcagta cttccggtat ctcgcgtttg tttgcctgca ggtcacgtaa taagtgtgcg
     1021 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     1081 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     1141 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     1201 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     1261 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     1321 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     1381 cgatcaattg cggccgctcg aatcgctatc caagccagct ccattgtcgg catcgtgctc
     1441 attctattga cgctggcaga tttggttttg gcgctatggg acccgttcgg ttacaacaac
     1501 atgtttccgc gcgagtttcc cgacgacatg tcgcgcacgt tcctgactgc gtactttgag
     1561 agtttcgaca acaccacgtc cagagaaatc atagagttta tgcccgagtt cttttcggaa
     1621 atggtcgaaa cggacgatga cgccacgttt gaatctctat ttcatttatt agattatgtg
     1681 gcatctttag aagttaattc cgacggccaa atgttaaact tggaggaggg tgatgaaatt
     1741 gaggattttg acgaatctac tttggtgggg caagcgttag ccactagctc gctatacact
     1801 cgcatggagt ttatgcagta cacgtttagg caaaacacac tattgtctat gaacaaagaa
     1861 aacaacaatt ttaatcaaat aatcatgggt ttatttgcaa caaacacaat tgtggcgttt
     1921 acagcatttg ttatacacac agaactcata ttttttatat ttttcgtaat cttcctaatg
     1981 atcacatttt attacataat caaagaatcg tacgaatatt ataaaacaat tgatttgtta
     2041 ttttaaaaac gattcatcct aggctacagg aacaggtggt ggcggccctc ggtgcgctcg
     2101 tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg
     2161 tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc
     2221 aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg
     2281 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
     2341 gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc
     2401 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     2461 tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc
     2521 ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc
     2581 agggggccgc ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc
     2641 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg
     2701 aagcgcatga actccttgat gacgttcttg gaggagcgca ccatggatcc caccagagac
     2761 aggttgcggc ggcggttgga tggcgtgggc gcgttggcgt tgttggaccg gctcatgttg
     2821 tgtcgctgta acagatgctg ttcaactgtg tttaccagat cgttgcgggc tgtatttata
     2881 ggcgcgataa gcgggacggg cgcctcgtgt ccggtcacgc gcatgagata acgcgcggct
     2941 gatatggagg cgcgtcctgt tccgataagg agttgcgtcc ggctgcggtt agcaacacag
     3001 gaagctggcg tcctgtcacg ataagacaac actcgtccgg tccgataatg tgattcgtac
     3061 gtgacaggac gcgacccgat aaggccggcc tacgtgactg ccgacacgta cttttttgca
     3121 ctgcaaaaag gttcaatgtg tggtagtgta tttggagcgt atacaacggt gtagactatt
     3181 tatgtaaaat agtctacgaa acgtagagtt tgtactatgt atgggcccgc gtgcaaaagc
     3241 gtgttttttt gcagtgcaaa aaagttggtg gtggggaggc caccgagtat gggcgcggta
     3301 cctcacgtaa taagtgtgcg ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag
     3361 taaaattttg ttgcatacct tatcaaaaaa taagtgctgc atacttttta gagaaaccaa
     3421 ataatttttt attgcatacc cgtttttaat aaaatacatt gcataccctc ttttaataaa
     3481 aaatattgca tactttgacg aaacaaattt tcgttgcata cccaataaaa gattattata
     3541 ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa aatattgcat
     3601 acgttgacga aacaaatttt cgttgcatac ccaataaaag attattatat tgcatacctt
     3661 ttcttgccat accatttagc cgatcaattc tcgagcgcgt aaaacacaat caagtatgag
     3721 tcataagctg atgtcatgtt ttgcacacgg ctcataaccg aactggcttt acgagtagaa
     3781 ttctacttgt aacgcacgat cagtggatga tgtcatttgt ttttcaaatc gagatgatgt
     3841 catgttttgc acacggctca taaactcgct ttacgagtag aattctacgt gtaacgcacg
     3901 atcgattgat gagtcatttg ttttgcaata tgatatcata caatatgact catttgtttt
     3961 tcaaaaccga acttgattta cgggtagaat tctacttgta aagcacaatc aaaaagatga
     4021 tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag aattctacgt gtaaaacaca
     4081 atcaagaaat gatgtcattt gttataaaaa taaaagctga tgtcatgttt tgcacatggc
     4141 tcataactaa actcgcttta cgggtagaat tctacgcgcg tcgatgtctt tgtgatgcgc
     4201 gcgacatttt tgtaggttat tgataaaatg aacggatacg ttgcccgaca ttatcattaa
     4261 atccttggcg tagaatttgt cgggtccatt gtccgtgtgc gctagcatgc ccgtaacgga
     4321 cctcgtactt ttggcttcaa aggttttgcg cacagacaaa atgtgccaca cttgcagctc
     4381 tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg tacttgttgt atgcaaataa
     4441 atctcgataa aggcgcggcg cgcgaatgca gctgatcacg tacgctcctc gtgttccgtt
     4501 caaggacggt gttatcgacc tcagattaat gtttatcggc cgactgtttt cgtatccgct
     4561 caccaaacgc gtttttgcat taacattgta tgtcggcgga tgttctatat ctaatttgaa
     4621 taaataaacg ataaccgcgt tggttttaga gggcataata aaagaaatat tgttatcgtg
     4681 ttcgccatta gggcagtata aattgacgtt catgttggat attgtttcag ttgcaagttg
     4741 acactggcgg cgacaagatc gtgaacaacc aagtgacaat acaaaatggt aattttaaaa
     4801 gcatattttt ttctttgaaa ttcataagtt atcaattatc gatggaaatg tattctatgg
     4861 agaacgtttt acccgatgaa tgggtgcaaa aattatttta ccttcaaatc tacaatcaac
     4921 acacgctaac ttttgtgact tgatcaactc tcacctggaa aagcaaccaa ctacaatcaa
     4981 cattctatgg gataatcgac aagtgagtaa aattatagcc ggacctctta gtacagtgta
     5041 tttaaaaggg gaataatatt ctatcaatag gaataaaaat aaggtcagca gccatgactt
     5101 ttccatcatt ttgaatatac cttatttgtt tcgggattaa ttgggggtcg gaaatcctct
     5161 tgaattcaga aacgggaacc ggaggaaggt gccggtcttt cagaaagctg tgaaaaatac
     5221 caacatttct gctgccaaga gctcaataag aagtttcaaa aattgtcttg gatgttgcag
     5281 ctgtggctgc taagtaataa gacatctatt agtatctaga tttgttagac catttaacat
     5341 agtgttttaa acgatggggt taatagatga gggttaagaa gctagttata ttactgttgc
     5401 tgtaacgcct tcaattgtcg gttacagagc aaacattatt gaatgttaat gtaaagagtt
     5461 tatttgtttt ctagtaaaca tatagcgatt ggttagtaat cactaataga aatttttcat
     5521 aagtatcaaa aaagtaaacc tctttttcag tctatgtaat aagtaaacca aggaaaggga
     5581 aaatatctac aatcaacaag ccattgttgc agcaacaaag caactgaaac tacaatcaac
     5641 attcaataaa cttgggtaat ttggaattta attctctggg acacctgtgg attacaacaa
     5701 tcaactcgaa acttattata caatgtaaat aaaaattgat atgcatacat gaagatcaag
     5761 tgaaattcca tttagaatca atttttttcg aatattaagt ttcttgcttt aatttatctg
     5821 aaagtaaata gacattccaa attcaagtta acaaattaat aatgaattga ctagtgattt
     5881 ttaagagaaa aagataagat ttaaaaaagg aaagcctttc ttgataaatt tttgaaccac
     5941 tttatgccgt ttcaatcata aaaactttta agaacacatg actggtaaaa ttaatttaaa
     6001 acaaatttaa attttcaacg taacattcaa caaaaatggt gaaaactatc acggaaattg
     6061 ttaatattaa tatgtcccaa aaatagcctt tgtatgtata tgatactaat ccatacatct
     6121 atggtatcta tagagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
     6181 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
     6241 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
     6301 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
     6361 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
     6421 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
     6481 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
     6541 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
     6601 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
     6661 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
     6721 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
     6781 catggtcctg ctggagttcg tgaccgccgc cgggatcact ctcggcatgg acgagctgta
     6841 caagtaacta gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     6901 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact
     6961 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     7021 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttaaa
     7081 gcttgtcgac gatgtaggtc acggtctcga agccgcggtg cgggtgccag ggcgtgccct
     7141 tgggctcccc gggcgcgtac tccacctcac ccatctggtc catcatgatg aacgggtcga
     7201 ggtggcggta gttgatcccg gcgaacgcgc ggcgcaccgg gaagccctcg ccctcgaaac
     7261 cgctgggcgc ggtggtcacg gtgagcacgg gacgtgcgac ggcgtcggcg ggtgcggata
     7321 cgcggggcag cgtcagcggg ttctcgacgg tcacggcggg catgtcgaca gatatctata
     7381 acaagaaaat atatatataa taagttatca cgtaagtaga acatgaaata acaatataat
     7441 tatcgtatga gttaaatctt aaaagtcacg taaaagataa tcatgcgtca ttttgactca
     7501 cgcggtcgtt atagttcaaa atcagtgaca cttaccgcat tgacaagcac gcctcacggg
     7561 agctccaagc ggcgactgag atgtcctaaa tgcacagcga cggattcgcg ctatttagaa
     7621 agagagagca atatttcaag aatgcatgcg tcaattttac gcagactatc tttctagggt
     7681 taatcccttt agtgagggtt aatttcgagc ttggcgtaat catggtcata gctgtttcct
     7741 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7801 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7861 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7921 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7981 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8041 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8101 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8161 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8221 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8281 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8341 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8401 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8461 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8521 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8581 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8641 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8701 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8761 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8821 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8881 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8941 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9001 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9061 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9121 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9181 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9241 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9301 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9361 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9421 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9481 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9541 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9601 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9661 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9721 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9781 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9841 ggggttccgc gcacatttcc ccgaaaagtg ccac
//
