LOCUS       sequence-407589-        8777 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Female specific eGFP with DmtraF, dsRed in both sexes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8777)
  AUTHORS   Liu J, Rayes D, Akbari OS
  TITLE     A fluorescent sex-sorting technique for insects with the 
            demonstration in Drosophila melanogaster.
  JOURNAL   bioRxiv. 2023 Aug 14:2023.08.11.553026. doi: 
            10.1101/2023.08.11.553026. Preprint.
  PUBMED    37645836
REFERENCE   2  (bases 1 to 8777)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Aug 14:2023.08.11.553026. doi: 10.1101/2023.08.11.553026. 
            Preprint."
COMMENT     Sequence Label: 795M
FEATURES             Location/Qualifiers
     source          1..8777
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   624..686
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1004..1389
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             complement(join(2064..2738,2739..2741,2742..2744))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(2126..2149)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2324..2343)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2541..2561
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     protein_bind    3304..3689
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     enhancer        3696..4178
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        4182..4773
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             5037..5750
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             5037..5747
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(5076..5097)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5337..5356)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5684..5705
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5860..5981
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5897..5916)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5951..5970
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    6009..6078
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     repeat_region   6548..6582
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(6589..6607)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(6628..6644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6628..6644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6641..6663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6652..6668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6676..6682,6683..6700,6701..6706))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6721..6742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6859..6876)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7030..7618)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7110..7129)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7789..8580,8581..8649))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8412..8431
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8650..8754)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtagat taaccctaga aagataatca tattgtgacg tacgttaaag
      661 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      721 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      781 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaactttc gttgatagaa
      841 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      901 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      961 tttttcagta cttccggtat ctcgcgtttg tttgcctgca ggtcacgtaa taagtgtgcg
     1021 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     1081 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     1141 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     1201 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     1261 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     1321 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     1381 cgatcaattg cggccgctcg aatcgctatc caagccagct ccattgtcgg catcgtgctc
     1441 attctattga cgctggcaga tttggttttg gcgctatggg acccgttcgg ttacaacaac
     1501 atgtttccgc gcgagtttcc cgacgacatg tcgcgcacgt tcctgactgc gtactttgag
     1561 agtttcgaca acaccacgtc cagagaaatc atagagttta tgcccgagtt cttttcggaa
     1621 atggtcgaaa cggacgatga cgccacgttt gaatctctat ttcatttatt agattatgtg
     1681 gcatctttag aagttaattc cgacggccaa atgttaaact tggaggaggg tgatgaaatt
     1741 gaggattttg acgaatctac tttggtgggg caagcgttag ccactagctc gctatacact
     1801 cgcatggagt ttatgcagta cacgtttagg caaaacacac tattgtctat gaacaaagaa
     1861 aacaacaatt ttaatcaaat aatcatgggt ttatttgcaa caaacacaat tgtggcgttt
     1921 acagcatttg ttatacacac agaactcata ttttttatat ttttcgtaat cttcctaatg
     1981 atcacatttt attacataat caaagaatcg tacgaatatt ataaaacaat tgatttgtta
     2041 ttttaaaaac gattcatcct aggctacagg aacaggtggt ggcggccctc ggtgcgctcg
     2101 tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg
     2161 tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc
     2221 aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg
     2281 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
     2341 gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc
     2401 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     2461 tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc
     2521 ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc
     2581 agggggccgc ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc
     2641 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg
     2701 aagcgcatga actccttgat gacgttcttg gaggagcgca ccatggatcc caccagagac
     2761 aggttgcggc ggcggttgga tggcgtgggc gcgttggcgt tgttggaccg gctcatgttg
     2821 tgtcgctgta acagatgctg ttcaactgtg tttaccagat cgttgcgggc tgtatttata
     2881 ggcgcgataa gcgggacggg cgcctcgtgt ccggtcacgc gcatgagata acgcgcggct
     2941 gatatggagg cgcgtcctgt tccgataagg agttgcgtcc ggctgcggtt agcaacacag
     3001 gaagctggcg tcctgtcacg ataagacaac actcgtccgg tccgataatg tgattcgtac
     3061 gtgacaggac gcgacccgat aaggccggcc tacgtgactg ccgacacgta cttttttgca
     3121 ctgcaaaaag gttcaatgtg tggtagtgta tttggagcgt atacaacggt gtagactatt
     3181 tatgtaaaat agtctacgaa acgtagagtt tgtactatgt atgggcccgc gtgcaaaagc
     3241 gtgttttttt gcagtgcaaa aaagttggtg gtggggaggc caccgagtat gggcgcggta
     3301 cctcacgtaa taagtgtgcg ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag
     3361 taaaattttg ttgcatacct tatcaaaaaa taagtgctgc atacttttta gagaaaccaa
     3421 ataatttttt attgcatacc cgtttttaat aaaatacatt gcataccctc ttttaataaa
     3481 aaatattgca tactttgacg aaacaaattt tcgttgcata cccaataaaa gattattata
     3541 ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa aatattgcat
     3601 acgttgacga aacaaatttt cgttgcatac ccaataaaag attattatat tgcatacctt
     3661 ttcttgccat accatttagc cgatcaattc tcgagcgcgt aaaacacaat caagtatgag
     3721 tcataagctg atgtcatgtt ttgcacacgg ctcataaccg aactggcttt acgagtagaa
     3781 ttctacttgt aacgcacgat cagtggatga tgtcatttgt ttttcaaatc gagatgatgt
     3841 catgttttgc acacggctca taaactcgct ttacgagtag aattctacgt gtaacgcacg
     3901 atcgattgat gagtcatttg ttttgcaata tgatatcata caatatgact catttgtttt
     3961 tcaaaaccga acttgattta cgggtagaat tctacttgta aagcacaatc aaaaagatga
     4021 tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag aattctacgt gtaaaacaca
     4081 atcaagaaat gatgtcattt gttataaaaa taaaagctga tgtcatgttt tgcacatggc
     4141 tcataactaa actcgcttta cgggtagaat tctacgcgcg tcgatgtctt tgtgatgcgc
     4201 gcgacatttt tgtaggttat tgataaaatg aacggatacg ttgcccgaca ttatcattaa
     4261 atccttggcg tagaatttgt cgggtccatt gtccgtgtgc gctagcatgc ccgtaacgga
     4321 cctcgtactt ttggcttcaa aggttttgcg cacagacaaa atgtgccaca cttgcagctc
     4381 tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg tacttgttgt atgcaaataa
     4441 atctcgataa aggcgcggcg cgcgaatgca gctgatcacg tacgctcctc gtgttccgtt
     4501 caaggacggt gttatcgacc tcagattaat gtttatcggc cgactgtttt cgtatccgct
     4561 caccaaacgc gtttttgcat taacattgta tgtcggcgga tgttctatat ctaatttgaa
     4621 taaataaacg ataaccgcgt tggttttaga gggcataata aaagaaatat tgttatcgtg
     4681 ttcgccatta gggcagtata aattgacgtt catgttggat attgtttcag ttgcaagttg
     4741 acactggcgg cgacaagatc gtgaacaacc aagtgacaat acaaaatggt acccactata
     4801 tagtataacc gatctgatca gattctgatc cagtgccatc tttttgttgt tttttttcta
     4861 gtgtcatatt gtgtgaaatg tgaaatggat gagaatgaac gctggacgac cagacagaga
     4921 aagagaagct aggacaatag gactctcaac tgcgcattac gtggattccg tctccgacga
     4981 tgcgccaaac actatgcgtt agatgcattg actaaccgca cactcttttc acatagagca
     5041 agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa
     5101 acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga
     5161 ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca
     5221 ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact
     5281 tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg
     5341 acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca
     5401 tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt
     5461 acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg
     5521 tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc
     5581 agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca
     5641 cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt
     5701 tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagtaa ctagatcata
     5761 atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     5821 ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     5881 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     5941 cattctagtt gtggtttgtc caaactcatc aatgtatctt aaagcttgtc gacgatgtag
     6001 gtcacggtct cgaagccgcg gtgcgggtgc cagggcgtgc ccttgggctc cccgggcgcg
     6061 tactccacct cacccatctg gtccatcatg atgaacgggt cgaggtggcg gtagttgatc
     6121 ccggcgaacg cgcggcgcac cgggaagccc tcgccctcga aaccgctggg cgcggtggtc
     6181 acggtgagca cgggacgtgc gacggcgtcg gcgggtgcgg atacgcgggg cagcgtcagc
     6241 gggttctcga cggtcacggc gggcatgtcg acagatatct ataacaagaa aatatatata
     6301 taataagtta tcacgtaagt agaacatgaa ataacaatat aattatcgta tgagttaaat
     6361 cttaaaagtc acgtaaaaga taatcatgcg tcattttgac tcacgcggtc gttatagttc
     6421 aaaatcagtg acacttaccg cattgacaag cacgcctcac gggagctcca agcggcgact
     6481 gagatgtcct aaatgcacag cgacggattc gcgctattta gaaagagaga gcaatatttc
     6541 aagaatgcat gcgtcaattt tacgcagact atctttctag ggttaatccc tttagtgagg
     6601 gttaatttcg agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     6661 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     6721 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     6781 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     6841 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     6901 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     6961 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     7021 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     7081 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     7141 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     7201 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     7261 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     7321 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     7381 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     7441 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     7501 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     7561 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     7621 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     7681 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     7741 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     7801 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     7861 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     7921 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     7981 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     8041 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     8101 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     8161 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     8221 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     8281 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     8341 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     8401 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     8461 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     8521 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     8581 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     8641 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     8701 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     8761 tccccgaaaa gtgccac
//
