LOCUS       sequence-407599-        8681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of miRFP670-T2A-mtq2 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8681)
  AUTHORS   Botman D, de Groot DH, Wehrens M, van Rossum K, Yska S, van der 
            Kieft B, Albert V, Minghetti N, Goedhart J, Teusink B
  TITLE     Side-by-side systematic characterization of novel FPs in budding 
            yeast
  JOURNAL   bioRxiv 2025.06.27.661975; doi: 
            https://doi.org/10.1101/2025.06.27.661975
REFERENCE   2  (bases 1 to 8681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.27.661975; doi: https://doi.org/10.1101/2025.06.27.661975"
COMMENT     Sequence Label: pDRF1-GW miRFP670-T2A-mtq2
FEATURES             Location/Qualifiers
     source          1..8681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1767..1820
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1836..1845
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1842..1844,1845..1847,1848..2561)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1887..1908)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2148..2167)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2495..2516
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2571..2595)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2657..2676)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2658..2676)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2824..3011
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2879..2903)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3023..3040)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3023..3039)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3032..3054)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3475..4821
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4932..5735)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5736..5951)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5976..5994)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6062..6166
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6167..6235,6236..7027)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6385..6404)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7198..7786
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7687..7706
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7940..7957
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8074..8095
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8110..8115,8116..8133,8134..8140)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8148..8164
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8153..8175
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8172..8188
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8172..8188
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8218..8673)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8355..8376)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8567..8586
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggcggaag
      841 gctccgtcgc caggcagcct gacctcttga cctgcgaaca tgaagagatc cacctcgccg
      901 gctcgatcca gccgcatggc gcgcttctgg tcgtcagcga acatgatcat cgcgtcatcc
      961 aggccagcgc caacgccgcg gaatttctga atctcggaag cgtactcggc gttccgctcg
     1021 ccgagatcga cggcgatctg ttgatcaaga tcctgccgca tctcgatccc accgccgaag
     1081 gcatgccggt cgcggtgcgc tgccggatcg gcaatccctc tacggagtac tgcggtctga
     1141 tgcatcggcc tccggaaggc gggctgatca tcgaactcga acgtgccggc ccgtcgatcg
     1201 atctgtcagg cacgctggcg ccggcgctgg agcggatccg cacggcgggt tcactgcgcg
     1261 cgctgtgcga tgacaccgtg ctgctgtttc agcagtgcac cggctacgac cgggtgatgg
     1321 tgtatcgttt cgatgagcaa ggccacggcc tggtattctc cgagtgccat gtgcctgggc
     1381 tcgaatccta tttcggcaac cgctatccgt cgtcgactgt cccgcagatg gcgcggcagc
     1441 tgtacgtgcg gcagcgcgtc cgcgtgctgg tcgacgtcac ctatcagccg gtgccgctgg
     1501 agccgcggct gtcgccgctg accgggcgcg atctcgacat gtcgggctgc ttcctgcgct
     1561 cgatgtcgcc gtgccatctg cagttcctga aggacatggg cgtgcgcgcc accctggcgg
     1621 tgtcgctggt ggtcggcggc aagctgtggg gcctggttgt ctgtcaccat tatctgccgc
     1681 gcttcatccg tttcgagctg cgggcgatct gcaaacggct cgccgaaagg atcgcgacgc
     1741 ggatcaccgc gcttgagagc tccggagagg gcagaggaag tcttctaaca tgcggtgacg
     1801 tggaggagaa tcccggccct ggggatccac cggtcgccac catggtgagc aagggcgagg
     1861 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     1921 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     1981 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgtcct
     2041 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     2101 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     2161 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     2221 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaactact
     2281 ttagcgacaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttca
     2341 agatccgcca caacatcgag gacggcggcg tgcagctcgc cgaccactac cagcagaaca
     2401 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca
     2461 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     2521 ccgccgggat cactctcggc atggacgagc tgtacaagta aagcggccgc acccagcttt
     2581 cttgtacaaa gtggtgtagt taattcatga tggccgctgc aggtcgacct cgaggggggg
     2641 cccggtaccc aattcgccct atagtgagtc gtattacgcg cggatccagc tttggacttc
     2701 ttcgccagag gtttggtcaa gtctccaatc aaggttgtcg gcttgtctac cttgccagaa
     2761 atttacgaaa agatggaaaa gggtcaaatc gttggtagat acgttgttga cacttctaaa
     2821 taagcgaatt tcttatgatt tatgattttt attattaaat aagttataaa aaaaataagt
     2881 gtatacaaat tttaaagtga ctcttaggtt ttaaaacgaa aattcttatt cttgagtaac
     2941 tctttcctgt aggtcaggtt gctttctcag gtatagcatg aggtcgctct tattgaccac
     3001 acctctaccg gcatgccaat tcactggccg tcgttttaca acgtcgtgac tgggaaaacc
     3061 ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata
     3121 gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc
     3181 gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataatcggat
     3241 cgtacttgtt acccatcatt gaattttgaa catccgaacc tgggagtttt ccctgaaaca
     3301 gatagtatat ttgaacctgt ataataatat atagtctagc gctttacgga agacaatgta
     3361 tgtatttcgg ttcctggaga aactattgca tctattgcat aggtaatctt gcacgtcgca
     3421 tccccggttc attttctgcg tttccatctt gcacttcaat agcatatctt tgttaacgaa
     3481 gcatctgtgc ttcattttgt agaacaaaaa tgcaacgcga gagcgctaat ttttcaaaca
     3541 aagaatctga gctgcatttt tacagaacag aaatgcaacg cgaaagcgct attttaccaa
     3601 cgaagaatct gtgcttcatt tttgtaaaac aaaaatgcaa cgcgagagcg ctaatttttc
     3661 aaacaaagaa tctgagctgc atttttacag aacagaaatg caacgcgaga gcgctatttt
     3721 accaacaaag aatctatact tcttttttgt tctacaaaaa tgcatcccga gagcgctatt
     3781 tttctaacaa agcatcttag attacttttt ttctcctttg tgcgctctat aatgcagtct
     3841 cttgataact ttttgcactg taggtccgtt aaggttagaa gaaggctact ttggtgtcta
     3901 ttttctcttc cataaaaaaa gcctgactcc acttcccgcg tttactgatt actagcgaag
     3961 ctgcgggtgc attttttcaa gataaaggca tccccgatta tattctatac cgatgtggat
     4021 tgcgcatact ttgtgaacag aaagtgatag cgttgatgat tcttcattgg tcagaaaatt
     4081 atgaacggtt tcttctattt tgtctctata tactacgtat aggaaatgtt tacattttcg
     4141 tattgttttc gattcactct atgaatagtt cttactacaa tttttttgtc taaagagtaa
     4201 tactagagat aaacataaaa aatgtagagg tcgagtttag atgcaagttc aaggagcgaa
     4261 aggtggatgg gtaggttata tagggatata gcacagagat atatagcaaa gagatacttt
     4321 tgagcaatgt ttgtggaagc ggtattcgca atattttagt agctcgttac agtccggtgc
     4381 gtttttggtt ttttgaaagt gcgtcttcag agcgcttttg gttttcaaaa gcgctctgaa
     4441 gttcctatac tttctagcta gagaatagga acttcggaat aggaacttca aagcgtttcc
     4501 gaaaacgagc gcttccgaaa atgcaacgcg agctgcgcac atacagctca ctgttcacgt
     4561 cgcacctata tctgcgtgtt gcctgtatat atatatacat gagaagaacg gcatagtgcg
     4621 tgtttatgct taaatgcgta cttatatgcg tctatttatg taggatgaaa ggtagtctag
     4681 tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc
     4741 ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat
     4801 catttccttt gatattggat cgatccgatg ataagctgtc aaacatgaga attgggtaat
     4861 aactgatata attaaattga agctctaatt tgtgagttta gtatacatgc atttacttat
     4921 aatacagttt tttagttttg ctggccgcat cttctcaaat atgcttccca gcctgctttt
     4981 ctgtaacgtt caccctctac cttagcatcc cttccctttg caaatagtcc tcttccaaca
     5041 ataataatgt cagatcctgt agagaccaca tcatccacgg ttctatactg ttgacccaat
     5101 gcgtctccct tgtcatctaa acccacaccg ggtgtcataa tcaaccaatc gtaaccttca
     5161 tctcttccac ccatgtctct ttgagcaata aagccgataa caaaatcttt gtcgctcttc
     5221 gcaatgtcaa cagtaccctt agtatattct ccagtagata gggagccctt gcatgacaat
     5281 tctgctaaca tcaaaaggcc tctaggttcc tttgttactt cttctgccgc ctgcttcaaa
     5341 ccgctaacaa tacctgggcc caccacaccg tgtgcattcg taatgtctgc ccattctgct
     5401 attctgtata cacccgcaga gtactgcaat ttgactgtat taccaatgtc agcaaatttt
     5461 ctgtcttcga agagtaaaaa attgtacttg gcggataatg cctttagcgg cttaactgtg
     5521 ccctccatgg aaaaatcagt caagatatcc acatgtgttt ttagtaaaca aattttggga
     5581 cctaatgctt caactaactc cagtaattcc ttggtggtac gaacatccaa tgaagcacac
     5641 aagtttgttt gcttttcgtg catgatatta aatagcttgg cagcaacagg actaggatga
     5701 gtagcagcac gttccttata tgtagctttc gacatgattt atcttcgttt cctgcatgtt
     5761 tttgttctgt gcagttgggt taagaatact gggcaatttc atgtttcttc aacactacat
     5821 atgcgtatat ataccaatct aagtctgtgc tccttccttc gttcttcctt ctgttcggag
     5881 attaccgaat caaaaaaatt tcaaggaaac cgaaatcaaa aaaaagaata aaaaaaaaat
     5941 gatgaattga aaagctaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata
     6001 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     6061 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     6121 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     6181 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     6241 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     6301 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     6361 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     6421 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     6481 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     6541 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     6601 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     6661 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     6721 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     6781 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     6841 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     6901 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     6961 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     7021 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     7081 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     7141 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     7201 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     7261 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     7321 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     7381 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     7441 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     7501 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     7561 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     7621 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     7681 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     7741 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     7801 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     7861 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     7921 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     7981 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     8041 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     8101 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     8161 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcttaccg catcaggaaa
     8221 ttgtaagcgt taatattttg ttaaaattcg cgttaaattt ttgttaaatc agctcatttt
     8281 ttaaccaata ggccgaaatc ggcaaaatcc cttataaatc aaaagaatag accgagatag
     8341 ggttgagtgt tgttccagtt tggaacaaga gtccactatt aaagaacgtg gactccaacg
     8401 tcaaagggcg aaaaaccgtc tatcagggcg atggcccact acgtgaacca tcaccctaat
     8461 caagtttttt ggggtcgagg tgccgtaaag cactaaatcg gaaccctaaa gggagccccc
     8521 gatttagagc ttgacgggga aagccggcga acgtggcgag aaaggaaggg aagaaagcga
     8581 aaggagcggg cgctagggcg ctggcaagtg tagcggtcac gctgcgcgta accaccacac
     8641 ccgccgcgct taatgcgccg ctacagggcg cgtccattcg c
//
