LOCUS       sequence-407600-       11395 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Female specific eGFP with AlutraF, dsRed in both sexes.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11395)
  AUTHORS   Liu J, Rayes D, Akbari OS
  TITLE     A fluorescent sex-sorting technique for insects with the 
            demonstration in Drosophila melanogaster.
  JOURNAL   bioRxiv. 2023 Aug 14:2023.08.11.553026. doi: 
            10.1101/2023.08.11.553026. Preprint.
  PUBMED    37645836
REFERENCE   2  (bases 1 to 11395)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv. 
            2023 Aug 14:2023.08.11.553026. doi: 10.1101/2023.08.11.553026. 
            Preprint."
COMMENT     Sequence Label: 795O
FEATURES             Location/Qualifiers
     source          1..11395
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(30..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     repeat_region   624..686
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     protein_bind    1004..1389
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     CDS             complement(join(2064..2738,2739..2741,2742..2744))
                     /codon_start=1
                     /product="wild-type DsRed"
                     /label=DsRed1
                     /note="mammalian codon-optimized"
                     /translation="MVRSSKNVIKEFMRFKVRMEGTVNGHEFEIEGEGEGRPYEGHNTV
                     KLKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGCFIYKVKFIGVNFPSDGPVMQKKTMGWEASTERLYPRDGVLKGEIH
                     KALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRH
                     HLFL"
     primer_bind     complement(2126..2149)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(2324..2343)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     2541..2561
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     protein_bind    3304..3689
                     /label=gypsy insulator
                     /bound_moiety="Su(Hw), CP190, andMod(mdg4)67.2"
                     /note="chromatin insulator from Drosophila"
     enhancer        3696..4178
                     /gene="Autographa californica hr5"
                     /label=hr5 enhancer
                     /note="baculovirus early transcription enhancer"
     promoter        4182..4773
                     /label=IE1 promoter
                     /note="promoter of the ie1 gene from the baculovirus 
                     Autographa californica"
     CDS             7655..8368
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     CDS             7655..8365
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDGN
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKVN
                     FKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEF
                     VTAAGITLGMDELYK"
     primer_bind     complement(7694..7715)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7955..7974)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8302..8323
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    8478..8599
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8515..8534)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8569..8588
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     protein_bind    8627..8696
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
     repeat_region   9166..9200
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(9207..9225)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(9246..9262)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9246..9262)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9259..9281)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9270..9286
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9294..9300,9301..9318,9319..9324))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9339..9360
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9477..9494)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9648..10236)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9728..9747)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10407..11198,11199..11267))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11030..11049
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11268..11372)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtagat taaccctaga aagataatca tattgtgacg tacgttaaag
      661 ataatcatgc gtaaaattga cgcatgtgtt ttatcggtct gtatatcgag gtttatttat
      721 taatttgaat agatattaag ttttattata tttacactta catactaata ataaattcaa
      781 caaacaattt atttatgttt atttatttat taaaaaaaaa caaaactttc gttgatagaa
      841 gcatcctcat cacaagatga taataagtat accatcttag ctggcttcgg tttatatgag
      901 acgagagtaa ggggtccgtc aaaacaaaac atcgatgttc ccactggcct ggagcgactg
      961 tttttcagta cttccggtat ctcgcgtttg tttgcctgca ggtcacgtaa taagtgtgcg
     1021 ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag taaaattttg ttgcatacct
     1081 tatcaaaaaa taagtgctgc atacttttta gagaaaccaa ataatttttt attgcatacc
     1141 cgtttttaat aaaatacatt gcataccctc ttttaataaa aaatattgca tactttgacg
     1201 aaacaaattt tcgttgcata cccaataaaa gattattata ttgcataccc gtttttaata
     1261 aaatacattg cataccctct tttaataaaa aatattgcat acgttgacga aacaaatttt
     1321 cgttgcatac ccaataaaag attattatat tgcatacctt ttcttgccat accatttagc
     1381 cgatcaattg cggccgctcg aatcgctatc caagccagct ccattgtcgg catcgtgctc
     1441 attctattga cgctggcaga tttggttttg gcgctatggg acccgttcgg ttacaacaac
     1501 atgtttccgc gcgagtttcc cgacgacatg tcgcgcacgt tcctgactgc gtactttgag
     1561 agtttcgaca acaccacgtc cagagaaatc atagagttta tgcccgagtt cttttcggaa
     1621 atggtcgaaa cggacgatga cgccacgttt gaatctctat ttcatttatt agattatgtg
     1681 gcatctttag aagttaattc cgacggccaa atgttaaact tggaggaggg tgatgaaatt
     1741 gaggattttg acgaatctac tttggtgggg caagcgttag ccactagctc gctatacact
     1801 cgcatggagt ttatgcagta cacgtttagg caaaacacac tattgtctat gaacaaagaa
     1861 aacaacaatt ttaatcaaat aatcatgggt ttatttgcaa caaacacaat tgtggcgttt
     1921 acagcatttg ttatacacac agaactcata ttttttatat ttttcgtaat cttcctaatg
     1981 atcacatttt attacataat caaagaatcg tacgaatatt ataaaacaat tgatttgtta
     2041 ttttaaaaac gattcatcct aggctacagg aacaggtggt ggcggccctc ggtgcgctcg
     2101 tactgctcca cgatggtgta gtcctcgttg tgggaggtga tgtccagctt ggagtccacg
     2161 tagtagtagc cgggcagctg cacgggcttc ttggccatgt agatggactt gaactccacc
     2221 aggtagtggc cgccgtcctt cagcttcagg gccttgtgga tctcgccctt cagcacgccg
     2281 tcgcgggggt acaggcgctc ggtggaggcc tcccagccca tggtcttctt ctgcattacg
     2341 gggccgtcgg aggggaagtt cacgccgatg aacttcacct tgtagatgaa gcagccgtcc
     2401 tgcagggagg agtcctgggt cacggtcacc acgccgccgt cctcgaagtt catcacgcgc
     2461 tcccacttga agccctcggg gaaggacagc ttcttgtagt cggggatgtc ggcggggtgc
     2521 ttcacgtaca ccttggagcc gtactggaac tggggggaca ggatgtccca ggcgaagggc
     2581 agggggccgc ccttggtcac cttcagcttc acggtgttgt ggccctcgta ggggcggccc
     2641 tcgccctcgc cctcgatctc gaactcgtgg ccgttcacgg tgccctccat gcgcaccttg
     2701 aagcgcatga actccttgat gacgttcttg gaggagcgca ccatggatcc caccagagac
     2761 aggttgcggc ggcggttgga tggcgtgggc gcgttggcgt tgttggaccg gctcatgttg
     2821 tgtcgctgta acagatgctg ttcaactgtg tttaccagat cgttgcgggc tgtatttata
     2881 ggcgcgataa gcgggacggg cgcctcgtgt ccggtcacgc gcatgagata acgcgcggct
     2941 gatatggagg cgcgtcctgt tccgataagg agttgcgtcc ggctgcggtt agcaacacag
     3001 gaagctggcg tcctgtcacg ataagacaac actcgtccgg tccgataatg tgattcgtac
     3061 gtgacaggac gcgacccgat aaggccggcc tacgtgactg ccgacacgta cttttttgca
     3121 ctgcaaaaag gttcaatgtg tggtagtgta tttggagcgt atacaacggt gtagactatt
     3181 tatgtaaaat agtctacgaa acgtagagtt tgtactatgt atgggcccgc gtgcaaaagc
     3241 gtgttttttt gcagtgcaaa aaagttggtg gtggggaggc caccgagtat gggcgcggta
     3301 cctcacgtaa taagtgtgcg ttgaatttat tcgcaaaaac attgcatatt ttcggcaaag
     3361 taaaattttg ttgcatacct tatcaaaaaa taagtgctgc atacttttta gagaaaccaa
     3421 ataatttttt attgcatacc cgtttttaat aaaatacatt gcataccctc ttttaataaa
     3481 aaatattgca tactttgacg aaacaaattt tcgttgcata cccaataaaa gattattata
     3541 ttgcataccc gtttttaata aaatacattg cataccctct tttaataaaa aatattgcat
     3601 acgttgacga aacaaatttt cgttgcatac ccaataaaag attattatat tgcatacctt
     3661 ttcttgccat accatttagc cgatcaattc tcgagcgcgt aaaacacaat caagtatgag
     3721 tcataagctg atgtcatgtt ttgcacacgg ctcataaccg aactggcttt acgagtagaa
     3781 ttctacttgt aacgcacgat cagtggatga tgtcatttgt ttttcaaatc gagatgatgt
     3841 catgttttgc acacggctca taaactcgct ttacgagtag aattctacgt gtaacgcacg
     3901 atcgattgat gagtcatttg ttttgcaata tgatatcata caatatgact catttgtttt
     3961 tcaaaaccga acttgattta cgggtagaat tctacttgta aagcacaatc aaaaagatga
     4021 tgtcatttgt ttttcaaaac tgaactcgct ttacgagtag aattctacgt gtaaaacaca
     4081 atcaagaaat gatgtcattt gttataaaaa taaaagctga tgtcatgttt tgcacatggc
     4141 tcataactaa actcgcttta cgggtagaat tctacgcgcg tcgatgtctt tgtgatgcgc
     4201 gcgacatttt tgtaggttat tgataaaatg aacggatacg ttgcccgaca ttatcattaa
     4261 atccttggcg tagaatttgt cgggtccatt gtccgtgtgc gctagcatgc ccgtaacgga
     4321 cctcgtactt ttggcttcaa aggttttgcg cacagacaaa atgtgccaca cttgcagctc
     4381 tgcatgtgtg cgcgttacca caaatcccaa cggcgcagtg tacttgttgt atgcaaataa
     4441 atctcgataa aggcgcggcg cgcgaatgca gctgatcacg tacgctcctc gtgttccgtt
     4501 caaggacggt gttatcgacc tcagattaat gtttatcggc cgactgtttt cgtatccgct
     4561 caccaaacgc gtttttgcat taacattgta tgtcggcgga tgttctatat ctaatttgaa
     4621 taaataaacg ataaccgcgt tggttttaga gggcataata aaagaaatat tgttatcgtg
     4681 ttcgccatta gggcagtata aattgacgtt catgttggat attgtttcag ttgcaagttg
     4741 acactggcgg cgacaagatc gtgaacaacc aagtgacaat acaaaatggt aattgtaacc
     4801 tacattttat tttatgaaat tcatataaaa ctaagcaata aggacgaaaa catactcggt
     4861 tgaatttata tgcgtgaaaa agatacaaaa atgcacgttt gcaaacacca atcaacattc
     4921 atcaatgata gtgatgtgga cgtcagattg gacaaaacta ggctacaatc aacatactaa
     4981 tggaagaatc ttataaactt aagtgagtac cattagataa gtctccaact aagaatttat
     5041 atcaagacga gcagtgctag aataggtcgt tttttgagaa atataattaa ctgtatcaga
     5101 aatatgcagt gcaattttac tcgagctact tagaagcctg ctctagatta aaagaatggg
     5161 gttggctttg gaattagggc atgcgatttt tgtgggtaaa tcgtggctgg taccgtagga
     5221 aagccggtgc cctggcctct ctaaataccc acttcgaccc acttggatgt cggaagcttg
     5281 atctcaattg ttttagtttc acacacaata tatttaaaac tgctggcatt tctgtaattt
     5341 aataattaat aattttccaa gtagcaaaac ttgcttgagt ttggcagaga aggaaagcat
     5401 tgcgagatag atgagtaaaa cacacacaaa cataagaatg ggaatgctag cacagataat
     5461 ggagaaataa aaattattac tatgtatatt taatcgtttc gatggcattt tcaaatggat
     5521 tctcatattt gcggcctatt gattggtaat taatttacct ttcgttacat attatatatg
     5581 tgtgcctgta ggtttatatc tataaaggat ttatattata tatacaaatt tcaatttcaa
     5641 gtacccttcg cggttctccg gggctcacct gagcagataa ccctctttct ctcatctcat
     5701 gtagcgaatc cgtccgaaac catcagacta caacaacaaa agcggtgcgc acaggcataa
     5761 gcaacgtaga caatcccatt tgccctttac cttgtaaacg taacacccct tccgagaatc
     5821 attaagattc aactttaacg gactccagag caagattgag aagataactc gatgaagaca
     5881 tcgaccacag ggacaattct ttagaatatc aaaatatagc agaatatgta caaaatatat
     5941 aatgtacata tatataccct ctgtcacgag ctgcctggca ggaaatcacc ctggtattgg
     6001 tgctcttatc agaggtgaaa accgattagt agctggtgac ttcaacgctt atcagaatct
     6061 taagcactca agcttgccaa tgatatgata caattttata ttctctcatt ttgaattcta
     6121 ttttctcatt tgcaacttaa ttttctcaat ttcaatttaa tcattctcat ttcaatatat
     6181 cattttcaat ttcatattca atctattttc ttattatgaa cttgaaaaga tgtagctgac
     6241 attgaaaaaa ccactctccc acagggactg aagaggttgt cctaagacac acatcaacaa
     6301 gagcaatgca ttttgttttt gactttggtc caaagaatgt taaaatctat aagcataaag
     6361 aagattgaca ctgtatagaa agcaaaatta acgttaaaca aaatttgaca aattggagac
     6421 catataaatg ataaaaatca aatgaactac tactgcctgt tcaatgtacc ttgggtgaac
     6481 cgcaaatttt ctatgtggtt gttgatgtag cagcattaac attccccata tttacatacg
     6541 gggattgctg ctggagtgac agtccttggc cggatataaa tccgggtcgt ttcggtaacg
     6601 tagaacccac tgtggtggaa acgtttttct atgtggtcga tactctctcc tcaattcgag
     6661 atcaaatctg caaaaagatg acgtaaagca aggggtgcca tagtagtgtc cttttcccct
     6721 tgcttttcaa cttctacatc catctttgcc ggaagagtct gctccctact cttactctat
     6781 gaggtaaatg ccgacaactg atgaccacag gcttggcagg ttacttaatt ccgaatgtta
     6841 gcgatcatac aataattatc ttaacactaa aaattgttta ttacttacaa taattaaatt
     6901 taatttaaat tgtttgtgag ggttttgtaa actgttaaaa caactacata catcttattt
     6961 tcatatctta aagtttcacc tagggagcgt ttatttaaag tctctgctgg agtcttgact
     7021 tgagtgagtt tagagctgat gaaatcaggg attatattgg gttcaaccca tattttagct
     7081 aaaatatcca actgaacata ttttacagtt tgtcgaataa gcaaagcaat taaaaacttc
     7141 aatcaacaaa ctacaatcaa caaactatta aagggaatac tggaacaaca acaaaatgaa
     7201 ctcaaactac aatggcaaac tgttggggaa ataaacatca tttgccacct gaggagaaca
     7261 acaatcaaca caaacgtttt taacaatgta aataaatata atgcttgata tacgaaatca
     7321 atacatgaag acatttttaa agttaactct cgactaccta cagtgcttta tttaaaatta
     7381 ctttcttcga tttattatta gcaaacaaac aaaagctagc tttaacattt tgaaaattca
     7441 ctttttcagc accatttggg ttgctatttt tattcacata atcaagcata aaacaaataa
     7501 attaattttg caatgattaa acgtatgtac acaaatacga aagaaatgca taagataaaa
     7561 ttactcgaca aaaaatggca tggaaaattg tagatataca aatcaaatga tattaattat
     7621 atactactaa tataaaaaat catcgtgccc acagagcaag ggcgaggagc tgttcaccgg
     7681 ggtggtgccc atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc
     7741 cggcgagggc gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac
     7801 cggcaagctg cccgtgccct ggcccaccct cgtgaccacc ctgacctacg gcgtgcagtg
     7861 cttcagccgc taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga
     7921 aggctacgtc caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc
     7981 cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt
     8041 caaggaggac ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt
     8101 ctatatcatg gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa
     8161 catcgaggac ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga
     8221 cggccccgtg ctgctgcccg acaaccacta cctgagcacc cagtccgccc tgagcaaaga
     8281 ccccaacgag aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac
     8341 tctcggcatg gacgagctgt acaagtaact agatcataat cagccatacc acatttgtag
     8401 aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga
     8461 atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata
     8521 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
     8581 aactcatcaa tgtatcttaa agcttgtcga cgatgtaggt cacggtctcg aagccgcggt
     8641 gcgggtgcca gggcgtgccc ttgggctccc cgggcgcgta ctccacctca cccatctggt
     8701 ccatcatgat gaacgggtcg aggtggcggt agttgatccc ggcgaacgcg cggcgcaccg
     8761 ggaagccctc gccctcgaaa ccgctgggcg cggtggtcac ggtgagcacg ggacgtgcga
     8821 cggcgtcggc gggtgcggat acgcggggca gcgtcagcgg gttctcgacg gtcacggcgg
     8881 gcatgtcgac agatatctat aacaagaaaa tatatatata ataagttatc acgtaagtag
     8941 aacatgaaat aacaatataa ttatcgtatg agttaaatct taaaagtcac gtaaaagata
     9001 atcatgcgtc attttgactc acgcggtcgt tatagttcaa aatcagtgac acttaccgca
     9061 ttgacaagca cgcctcacgg gagctccaag cggcgactga gatgtcctaa atgcacagcg
     9121 acggattcgc gctatttaga aagagagagc aatatttcaa gaatgcatgc gtcaatttta
     9181 cgcagactat ctttctaggg ttaatccctt tagtgagggt taatttcgag cttggcgtaa
     9241 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     9301 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     9361 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     9421 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     9481 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     9541 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     9601 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
     9661 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     9721 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     9781 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     9841 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     9901 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     9961 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
    10021 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
    10081 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
    10141 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
    10201 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
    10261 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
    10321 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
    10381 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
    10441 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
    10501 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
    10561 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
    10621 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
    10681 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
    10741 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
    10801 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
    10861 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
    10921 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
    10981 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
    11041 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
    11101 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
    11161 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
    11221 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
    11281 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
    11341 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccac
//
