LOCUS       sequence-407624-        8714 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of miFP663-T2A-mtq2 in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8714)
  AUTHORS   Botman D, de Groot DH, Wehrens M, van Rossum K, Yska S, van der 
            Kieft B, Albert V, Minghetti N, Goedhart J, Teusink B
  TITLE     Side-by-side systematic characterization of novel FPs in budding 
            yeast
  JOURNAL   bioRxiv 2025.06.27.661975; doi: 
            https://doi.org/10.1101/2025.06.27.661975
REFERENCE   2  (bases 1 to 8714)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.27.661975; doi: https://doi.org/10.1101/2025.06.27.661975"
COMMENT     Sequence Label: pDRF1-GW miFP663-T2A-mtq2
FEATURES             Location/Qualifiers
     source          1..8714
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1800..1853
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1869..1878
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1875..1877,1878..1880,1881..2594)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1920..1941)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2181..2200)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2528..2549
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2604..2628)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2690..2709)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2691..2709)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2857..3044
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2912..2936)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3056..3073)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3056..3072)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3065..3087)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3508..4854
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4965..5768)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5769..5984)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(6009..6027)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6095..6199
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6200..6268,6269..7060)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6418..6437)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7231..7819
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7720..7739
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7973..7990
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8107..8128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8143..8148,8149..8166,8167..8173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8181..8197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8186..8208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8205..8221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8205..8221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8251..8706)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8388..8409)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8600..8619
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggtgagca
      841 ccgctaccga tcccctcgac ctggatgtgt gtgccagaga gcccatccac atcccaggac
      901 tgatccagcc ttacggcgcg ctgctcgtgg tcgaccctgc cgacggaaga atcgtgcagg
      961 cctctacaac agcagccgat cttctgggag tgcctatggc tgcgctgctg ggcatgcctt
     1021 acacccaggt gctgacactg cctgaggcac agcccttcgc cgtggacgat cagccacagc
     1081 acctgatgca tgccgaggtg cggttccctc ggagagccac acctcctgcc tctgcttatg
     1141 tggctgcctg gcatctttat ccccagcagt ggctggtgga aattgaaccc ggggacgcca
     1201 gactgctgga caccgtgctg agagaagccc tgcctctcct gcggagcgtg gagagagatc
     1261 ccggaattgc cgaagccgct gtgcgggtgg ccaagggcct gagatctctg atcggcttcg
     1321 accgcgtgat gatctacaga ttcgacgagg aatggaacgg cgacatcatt gccgaggctc
     1381 ggaagcctga gctggaagcc tatctgggac tccactaccc tgccagcctg acccctgctc
     1441 tggccagagc cctgtacctg cggaatagag tgcggcagat cgccgacgtg ggctaccagc
     1501 ctagccctat ccagcctacc atgcaccctc agctgggcac ccctgtggat ctgtccgatg
     1561 tgtccctgag aagcatgtct ccatgccacc tggaattcct ggccaacatc ggtgtgagcg
     1621 ctaccctggt cgccagcatc gtcgtgaacg atgccctgtg gggactgatc gtctgccacc
     1681 actacagccc aagattcacc aaccacgccc tgcgcgcctt gacagacgcc gtggctaaaa
     1741 cactggctgc cagaattggc gccctgcagg ctgaggccag agccaggctg gagtccggag
     1801 agggcagagg aagtcttcta acatgcggtg acgtggagga gaatcccggc cctggggatc
     1861 caccggtcgc caccatggtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc
     1921 tggtcgagct ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg
     1981 gcgatgccac ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg
     2041 tgccctggcc caccctcgtg accaccctgt cctggggcgt gcagtgcttc gcccgctacc
     2101 ccgaccacat gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg
     2161 agcgcaccat cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg
     2221 agggcgacac cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca
     2281 acatcctggg gcacaagctg gagtacaact actttagcga caacgtctat atcaccgccg
     2341 acaagcagaa gaacggcatc aaggccaact tcaagatccg ccacaacatc gaggacggcg
     2401 gcgtgcagct cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc
     2461 tgcccgacaa ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc
     2521 gcgatcacat ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg
     2581 agctgtacaa gtaaagcggc cgcacccagc tttcttgtac aaagtggtgt agttaattca
     2641 tgatggccgc tgcaggtcga cctcgagggg gggcccggta cccaattcgc cctatagtga
     2701 gtcgtattac gcgcggatcc agctttggac ttcttcgcca gaggtttggt caagtctcca
     2761 atcaaggttg tcggcttgtc taccttgcca gaaatttacg aaaagatgga aaagggtcaa
     2821 atcgttggta gatacgttgt tgacacttct aaataagcga atttcttatg atttatgatt
     2881 tttattatta aataagttat aaaaaaaata agtgtataca aattttaaag tgactcttag
     2941 gttttaaaac gaaaattctt attcttgagt aactctttcc tgtaggtcag gttgctttct
     3001 caggtatagc atgaggtcgc tcttattgac cacacctcta ccggcatgcc aattcactgg
     3061 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     3121 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     3181 cccaacagtt gcgcagcctg aatggcgaat ggcgcctgat gcggtatttt ctccttacgc
     3241 atctgtgcgg tatttcacac cgcataatcg gatcgtactt gttacccatc attgaatttt
     3301 gaacatccga acctgggagt tttccctgaa acagatagta tatttgaacc tgtataataa
     3361 tatatagtct agcgctttac ggaagacaat gtatgtattt cggttcctgg agaaactatt
     3421 gcatctattg cataggtaat cttgcacgtc gcatccccgg ttcattttct gcgtttccat
     3481 cttgcacttc aatagcatat ctttgttaac gaagcatctg tgcttcattt tgtagaacaa
     3541 aaatgcaacg cgagagcgct aatttttcaa acaaagaatc tgagctgcat ttttacagaa
     3601 cagaaatgca acgcgaaagc gctattttac caacgaagaa tctgtgcttc atttttgtaa
     3661 aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa gaatctgagc tgcattttta
     3721 cagaacagaa atgcaacgcg agagcgctat tttaccaaca aagaatctat acttcttttt
     3781 tgttctacaa aaatgcatcc cgagagcgct atttttctaa caaagcatct tagattactt
     3841 tttttctcct ttgtgcgctc tataatgcag tctcttgata actttttgca ctgtaggtcc
     3901 gttaaggtta gaagaaggct actttggtgt ctattttctc ttccataaaa aaagcctgac
     3961 tccacttccc gcgtttactg attactagcg aagctgcggg tgcatttttt caagataaag
     4021 gcatccccga ttatattcta taccgatgtg gattgcgcat actttgtgaa cagaaagtga
     4081 tagcgttgat gattcttcat tggtcagaaa attatgaacg gtttcttcta ttttgtctct
     4141 atatactacg tataggaaat gtttacattt tcgtattgtt ttcgattcac tctatgaata
     4201 gttcttacta caattttttt gtctaaagag taatactaga gataaacata aaaaatgtag
     4261 aggtcgagtt tagatgcaag ttcaaggagc gaaaggtgga tgggtaggtt atatagggat
     4321 atagcacaga gatatatagc aaagagatac ttttgagcaa tgtttgtgga agcggtattc
     4381 gcaatatttt agtagctcgt tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt
     4441 cagagcgctt ttggttttca aaagcgctct gaagttccta tactttctag ctagagaata
     4501 ggaacttcgg aataggaact tcaaagcgtt tccgaaaacg agcgcttccg aaaatgcaac
     4561 gcgagctgcg cacatacagc tcactgttca cgtcgcacct atatctgcgt gttgcctgta
     4621 tatatatata catgagaaga acggcatagt gcgtgtttat gcttaaatgc gtacttatat
     4681 gcgtctattt atgtaggatg aaaggtagtc tagtacctcc tgtgatatta tcccattcca
     4741 tgcggggtat cgtatgcttc cttcagcact accctttagc tgttctatat gctgccactc
     4801 ctcaattgga ttagtctcat ccttcaatgc tatcatttcc tttgatattg gatcgatccg
     4861 atgataagct gtcaaacatg agaattgggt aataactgat ataattaaat tgaagctcta
     4921 atttgtgagt ttagtataca tgcatttact tataatacag ttttttagtt ttgctggccg
     4981 catcttctca aatatgcttc ccagcctgct tttctgtaac gttcaccctc taccttagca
     5041 tcccttccct ttgcaaatag tcctcttcca acaataataa tgtcagatcc tgtagagacc
     5101 acatcatcca cggttctata ctgttgaccc aatgcgtctc ccttgtcatc taaacccaca
     5161 ccgggtgtca taatcaacca atcgtaacct tcatctcttc cacccatgtc tctttgagca
     5221 ataaagccga taacaaaatc tttgtcgctc ttcgcaatgt caacagtacc cttagtatat
     5281 tctccagtag atagggagcc cttgcatgac aattctgcta acatcaaaag gcctctaggt
     5341 tcctttgtta cttcttctgc cgcctgcttc aaaccgctaa caatacctgg gcccaccaca
     5401 ccgtgtgcat tcgtaatgtc tgcccattct gctattctgt atacacccgc agagtactgc
     5461 aatttgactg tattaccaat gtcagcaaat tttctgtctt cgaagagtaa aaaattgtac
     5521 ttggcggata atgcctttag cggcttaact gtgccctcca tggaaaaatc agtcaagata
     5581 tccacatgtg tttttagtaa acaaattttg ggacctaatg cttcaactaa ctccagtaat
     5641 tccttggtgg tacgaacatc caatgaagca cacaagtttg tttgcttttc gtgcatgata
     5701 ttaaatagct tggcagcaac aggactagga tgagtagcag cacgttcctt atatgtagct
     5761 ttcgacatga tttatcttcg tttcctgcat gtttttgttc tgtgcagttg ggttaagaat
     5821 actgggcaat ttcatgtttc ttcaacacta catatgcgta tatataccaa tctaagtctg
     5881 tgctccttcc ttcgttcttc cttctgttcg gagattaccg aatcaaaaaa atttcaagga
     5941 aaccgaaatc aaaaaaaaga ataaaaaaaa aatgatgaat tgaaaagcta attcttgaag
     6001 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     6061 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     6121 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     6181 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     6241 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     6301 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     6361 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     6421 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     6481 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     6541 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     6601 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     6661 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     6721 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     6781 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     6841 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     6901 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     6961 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     7021 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     7081 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     7141 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     7201 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     7261 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     7321 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     7381 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     7441 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     7501 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     7561 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     7621 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     7681 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     7741 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     7801 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     7861 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     7921 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     7981 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     8041 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     8101 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     8161 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     8221 ccatgattac gccaagctta ccgcatcagg aaattgtaag cgttaatatt ttgttaaaat
     8281 tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa atcggcaaaa
     8341 tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca gtttggaaca
     8401 agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg
     8461 gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg aggtgccgta
     8521 aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg ggaaagccgg
     8581 cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa
     8641 gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg ccgctacagg
     8701 gcgcgtccat tcgc
//
