LOCUS       sequence-407645-        8576 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of SARS2 coronavirus S protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8576)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 8576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_SARS-CoV-2_Sdel18
FEATURES             Location/Qualifiers
     source          1..8576
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     complement(5281..5300)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    5346..5401
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5400..5419)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5759..5775)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5759..5775)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5772..5794)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5783..5799
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5807..5813,5814..5831,5832..5837))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5852..5873
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5990..6007)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6161..6749)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6241..6260)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6920..7711,7712..7780))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7543..7562
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7781..7885)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7911..8366)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8048..8069)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8260..8279
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8493..8515
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8507..8524
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8508..8524
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8534..8553
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8534..8552
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcct cgacggatct gggccgcacc
     1441 atgttcgttt ttcttgttct tcttccattg gtaagctcac aatgcgtcaa tcttacaacg
     1501 cgaacacaac tcccaccagc ttataccaat agcttcacgc gaggagtata ttatccggac
     1561 aaagttttca gaagctccgt cctccattca acacaagacc tcttccttcc tttcttctcc
     1621 aacgtcacgt ggtttcacgc tatacacgtc tctggaacca acggtacaaa acgcttcgac
     1681 aaccccgtac tccctttcaa cgacggggtt tattttgctt caacggaaaa atcaaacatt
     1741 atacgcggtt ggatcttcgg gaccactctt gattcaaaaa cgcagagctt gctcatcgtc
     1801 aacaacgcca ctaatgtagt aattaaggtg tgcgagttcc aattttgcaa cgatcctttc
     1861 ttgggtgttt attatcataa aaataacaaa agctggatgg agagcgaatt tagagtctac
     1921 tcctccgcca ataactgtac attcgagtat gtaagccagc cctttttgat ggacctggaa
     1981 ggcaagcaag gaaacttcaa aaacttgcgc gaattcgtgt tcaaaaacat agacgggtat
     2041 tttaaaattt atagtaagca cacacctatt aacttggtgc gggacctgcc tcaaggattc
     2101 tccgccttgg aaccgcttgt agatctgcca ataggaatta acattacacg attccagacc
     2161 cttctcgccc tgcacaggag ttatctgacg ccaggcgatt cttcttctgg ctggaccgcc
     2221 ggcgctgccg catattacgt gggttacctt cagcctagga cattccttct gaaatacaac
     2281 gaaaacggta caataactga tgcggttgat tgcgccctgg acccgctcag cgagacaaag
     2341 tgcaccttga agtctttcac ggtcgagaaa ggaatatacc aaacaagcaa ctttcgcgtg
     2401 caacctaccg agtcaatcgt ccgattcccg aatataacca acctgtgtcc atttggtgaa
     2461 gtattcaatg cgacacggtt tgctagtgta tatgcctgga accggaaacg aatttctaac
     2521 tgtgtagccg actattctgt cctgtacaac tctgcgagct tctccacatt taagtgttac
     2581 ggagtaagtc ctaccaaact gaatgatctc tgcttcacca acgtttatgc cgacagcttt
     2641 gtaattagag gggacgaggt gcggcaaatc gccccagggc agacgggcaa aatagccgac
     2701 tacaattaca agctccccga tgatttcaca ggatgcgtga tagcttggaa ttccaataac
     2761 ttggatagta aagtgggagg gaattataat tatctctacc gattgttcag gaaatccaac
     2821 ctcaagccgt ttgaacgcga cataagtacc gagatttatc aggcagggtc tacgccgtgt
     2881 aatggtgtag aaggatttaa ctgctacttt ccacttcagt catatggctt tcagccgact
     2941 aatggtgtag ggtatcagcc atacagagta gtagtcctgt cttttgaatt gctccacgcg
     3001 ccagccacag tgtgcggccc aaagaagtca acgaaccttg tcaaaaataa atgcgtgaac
     3061 ttcaatttca acggcctgac gggaaccggc gttttgacag aaagtaataa aaaattcctc
     3121 ccgttccaac agttcgggcg ggatatagcg gataccaccg atgcagtacg agatccgcag
     3181 accctggaga ttctggacat aacgccttgt tccttcggcg gtgtcagtgt gataacccct
     3241 gggacaaata cctctaacca agtcgctgtg ctttatcaag gcgtgaattg cacagaggtg
     3301 ccagttgcca tacatgctga tcagttgact ccaacctggc gggtctattc caccggttca
     3361 aatgttttcc agacccgcgc tggctgtttg attggggcag agcatgtgaa caactcttac
     3421 gagtgcgata tacctatagg tgctgggatc tgcgcttctt accagaccca gacgaatagc
     3481 ccccgacggg ccagaagtgt tgcaagtcag tcaatcattg cgtacacaat gtctcttgga
     3541 gcggaaaatt ctgtggccta cagcaataat agcattgcta ttccgactaa ttttacgatt
     3601 agcgtgacaa ccgaaatctt gccggtgagt atgactaaga ccagcgtcga ctgtacgatg
     3661 tatatttgtg gagactccac ggagtgtagt aatctcttgc tccagtatgg atccttctgc
     3721 acacagctga atcgggctct caccggcatt gccgtagaac aggataaaaa tacccaagag
     3781 gtctttgcgc aggtcaaaca aatatacaaa acgccgccga taaaggattt cggtgggttt
     3841 aactttagcc agattttgcc ggatccgtct aagccctcta aacgcagttt catagaagat
     3901 ttgttgttca ataaagtcac gcttgcagat gctggtttta tcaagcaata cggggattgt
     3961 cttggcgata ttgcagcacg ggacctgatc tgcgcacaga aattcaacgg actcacagtc
     4021 cttcccccgc ttctgaccga tgagatgata gcacaataca cgagcgcact tttggccggc
     4081 acgataactt ccgggtggac ttttggtgca ggagcggctc tgcaaatacc cttcgctatg
     4141 caaatggctt accgatttaa cggaattgga gttactcaga acgttcttta cgaaaaccaa
     4201 aaacttattg ccaatcaatt caatagtgct attgggaaga tacaggattc tctctctagt
     4261 accgccagcg ctctggggaa actgcaagat gtcgtcaatc aaaatgcgca agcccttaac
     4321 acactcgtca agcaactttc ttcaaatttt ggagccatct cttccgtcct taatgatata
     4381 ctttccaggc tggacaaggt agaagccgaa gtacagattg accggcttat aacggggaga
     4441 ctgcaatcct tgcaaacata cgtcacacag cagcttatcc gagctgccga gattagagcg
     4501 agtgcgaatc tcgccgccac taaaatgtca gaatgtgtcc ttgggcagag taaacgggta
     4561 gatttttgcg gtaagggtta ccatcttatg tcattcccgc aaagcgctcc ccacggggtc
     4621 gtttttctcc atgttaccta tgtccccgct caggagaaaa acttcacaac tgcgccggcc
     4681 atatgtcatg acggtaaggc gcattttcct cgagaaggcg tgttcgtatc caatggcaca
     4741 cactggttcg ttacacagcg caatttttat gaaccccaga ttattacgac tgataatact
     4801 ttcgtatcag gtaactgcga cgtagtgatc ggcatcgtaa acaataccgt ctacgaccca
     4861 ttgcagccgg aattggattc atttaaggag gagctcgata aatactttaa gaatcacacc
     4921 agcccggacg tcgatttggg agatatttca gggatcaatg ctagcgtagt aaatattcaa
     4981 aaggagattg accgacttaa cgaggtcgcc aaaaatctga atgagtcact tattgacctt
     5041 caggaactgg gtaaatacga acagtatatc aaatggccct ggtatatatg gctcgggttt
     5101 atcgccggac tcatagcaat cgtaatggtc accattatgt tgtgctgtat gacttcttgt
     5161 tgcagctgtc tcaaagggtg ttgttcctgt ggctcttgct gtaaatgatc aggtgcaggc
     5221 tgcctatcag aaggtggtgg ctggtgtggc caatgccctg gctcacaaat accactgaga
     5281 tctttttccc tctgccaaaa attatgggga catcatgaag ccccttgagc atctgacttc
     5341 tggctaataa aggaaattta ttttcattgc aatagtgtgt tggaattttt tgtgtctctc
     5401 actcggaagg acatatggga gggcaaatca tttaaaacat cagaatgagt atttggttta
     5461 gagtttggca acatatgccc atatgctggc tgccatgaac aaaggttggc tataaagagg
     5521 tcatcagtat atgaaacagc cccctgctgt ccattcctta ttccatagaa aagccttgac
     5581 ttgaggttag atttttttta tattttgttt tgtgttattt ttttctttaa catccctaaa
     5641 attttcctta catgttttac tagccagatt tttcctcctc tcctgactac tcccagtcat
     5701 agctgtccct cttctcttat ggagatccct cgacggatcg gccgcaattc gtaatcatgt
     5761 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg
     5821 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt
     5881 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg
     5941 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg
     6001 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa
     6061 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc
     6121 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
     6181 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
     6241 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
     6301 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
     6361 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
     6421 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
     6481 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
     6541 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
     6601 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
     6661 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
     6721 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
     6781 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga
     6841 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg
     6901 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct
     6961 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg
     7021 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggcac
     7081 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa
     7141 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc
     7201 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt
     7261 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc
     7321 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt
     7381 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc
     7441 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt
     7501 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata
     7561 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga
     7621 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag
     7681 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa
     7741 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt
     7801 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga
     7861 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag
     7921 cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca
     7981 ataggccgaa atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag
     8041 tgttgttcca gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg
     8101 gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt
     8161 tttggggtcg aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag
     8221 agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc
     8281 gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc
     8341 gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc aggctgcgca actgttggga
     8401 agggcgatcg gtgcgggcct cttcgctatt acgccagctg gcgaaagggg gatgtgctgc
     8461 aaggcgatta agttgggtaa cgccagggtt ttcccagtca cgacgttgta aaacgacggc
     8521 cagtgagcgc gcgtaatacg actcactata gggcgaattg gagctccacc gcggtg
//
