LOCUS       sequence-407650-        8208 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Puumala virus GP protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8208)
  AUTHORS   Strebinger D, Frangieh CJ, Friedrich MJ, Faure G, Macrae RK, Zhang F
  TITLE     Cell type-specific delivery by modular envelope design.
  JOURNAL   Nat Commun. 2023 Aug 23;14(1):5141. doi: 10.1038/s41467-023-40788-8.
  PUBMED    37612276
REFERENCE   2  (bases 1 to 8208)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-08-23"; volume: "14(1):5141. doi"; pages: " 
            10.1038/s41467-023-40788-8"
COMMENT     Sequence Label: pHCMV_PUUV_GP
FEATURES             Location/Qualifiers
     source          1..8208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        97..476
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        477..680
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     631..651
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     677..701
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          792..1364
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     regulatory      1419..1428
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(4913..4932)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    4978..5033
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(5032..5051)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5391..5407)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5391..5407)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    5415..5431
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5439..5445,5446..5463,5464..5469))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5484..5505
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5622..5639)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5793..6381)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5873..5892)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6552..7343,7344..7412))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7175..7194
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7413..7517)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(7543..7998)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7680..7701)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7892..7911
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8125..8147
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8139..8156
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8140..8156
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8166..8185
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8166..8184
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 gcggccgctc tagagagctt ggcccattgc atacgttgta tccatatcat aatatgtaca
       61 tttatattgg ctcatgtcca acattaccgc catgttgaca ttgattattg actagttatt
      121 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
      181 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
      241 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg
      301 agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc
      361 cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct
      421 tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga
      481 tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa
      541 gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc
      601 caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg
      661 aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaca cgccatccac
      721 gctgttttga cctccataga tgacaccggg accgatccag cctccggtcg accgatcctg
      781 agaacttcag ggtgagtttg gggacccttg attgttcttt ctttttcgct attgtaaaat
      841 tcatgttata tggagggggc aaagttttca gggtgttgtt tagaatggga agatgtccct
      901 tgtatcacca tggaccctca tgataatttt gtttctttca ctttctactc tgttgacaac
      961 cattgtctcc tcttattttc ttttcatttt ctgtaacttt ttcgttaaac tttagcttgc
     1021 atttgtaacg aatttttaaa ttcacttttg tttatttgtc agattgtaag tactttctct
     1081 aatcactttt ttttcaaggc aatcagggta tattatattg tacttcagca cagttttaga
     1141 gaacaattgt tataattaaa tgataaggta gaatatttct gcatataaat tctggctggc
     1201 gtggaaatat tcttattggt agaaacaact acaccctggt catcatcctg cctttctctt
     1261 tatggttaca atgatataca ctgtttgaga tgaggataaa atactctgag tccaaaccgg
     1321 gcccctctgc taaccatgtt catgccttct tctctttcct acagctcctg ggcaacgtgc
     1381 tggttgttgt gctgtctcat cattttggca aagaattcgc caccatggga aaaagctccc
     1441 ccgtgtgtct gtatctgatt ctgcaaggcc tcctcctctt cgacaccgtg aatgccaaaa
     1501 atctgaacga gctcaaaatg gagtgtcccc acacaattgg actgggacaa ggactggtgg
     1561 tgggcagcgt ggaactgccc cccgtgccca ttcagcaaat cgagtctctg aagctcgaga
     1621 gcagctgcaa tttcgatctg catacatcca ccgccggcca gcaaagcttc accaagtgga
     1681 catgggagac aaagggcgac ctcgctgaaa atacccaagc cagcagcacc tcctttcaaa
     1741 ccaagagctc cgaagtgaat ctgagaggcc tctgtctgat tcctacactg gtggtggaaa
     1801 cagccgctag aatgagaaag accattgctt gctacgatct gagctgcaac caaaccgtgt
     1861 gtcaacctac cgtctatctg atgggaccta tccaaacatg tctgaccacc aagagctgtc
     1921 tgctcggact gggcgaccaa aggatccaag tgaactacga gaggacatac tgtgtcagcg
     1981 gacagctggt ggaaggcgtc tgcttcaacc ccatccatac catggctctg tcccagccta
     2041 gccacaccta cgatattgtc accatcatgg tgagatgctt tctggtgatc aaaaaggtga
     2101 cctccggcga cagcatgaaa atcgagaaga acttcgaaac actggtccag aaaaccggct
     2161 gtacagccaa tggcttccaa ggctactata tttgtctgat cggctctagc agcgaacctc
     2221 tgtatgtccc cacactggac gattatagaa gcgctgaggt cctctctaga atggcttttg
     2281 ccccccacgg agaggaccac gacatcgaaa agaacgccgt gagcgctctg aggattgctg
     2341 gcaaggtgac cggcaaggcc ccctccaccg agagctccga caccgtgcaa ggcattgcct
     2401 tctccggctc ccctctgtac acatccaccg gcgtgctgac cgccaaggat gatcccgtct
     2461 acgtctgggc tcccggcatc attatggagg gaaaccactc cgtgtgtgag aaaaagacac
     2521 tgcctctgac atggaccggc ttcatccctc tgcccggaga gattgaaaag accacccagt
     2581 gcaccgtgtt ctgcaccctc gctggccccg gcgccgattg tgaggcctac tccgagaccg
     2641 gcatctttaa cattagcagc cccacatgtc tgattaatag ggtccagaga tttagaggcg
     2701 ccgaacaaca gatcaagttc gtctgccaga gggtcgacat ggatattaca gtctactgta
     2761 acggcgtcaa gaaggtcatt ctgacaaaga ccctcgtcat cggccaatgt atttacacct
     2821 tcaccagcat cttctccatg atccccggca ttgctcacag cctcgctgtg gaactgtgtg
     2881 tgcccggact ccatggctgg gccacagtgc tgctgctgct gaccttctgc ttcggctggg
     2941 tgctgattcc caccatcacc atgattctgc tgaagattct gatcgccttc gcctatctct
     3001 gcagcaagta caacaccgat tccaagttca gaatcctcgt ggaaaaggtc aagaaagagt
     3061 accaaaagac catgggatcc atggtgtgcg aggtctgtca atacgagtgc gagacagcca
     3121 aggaactcga aagccataga aaaagctgct ccatcggaag ctgtccttac tgtctcaacc
     3181 cctccgaagc cacccctagc gctctgcaag cccactttaa ggtctgtaag ctcacaagca
     3241 gattccaaga gaatctgaaa aagtccctca ccatgtacga gcctatgcaa ggctgctata
     3301 gaaccctctc tctgtttagg tatagatcca gattctttgt gggactggtc tggtgtatgc
     3361 tcctcgtgct cgagctcatt gtgtgggccg cctccgccga gacacagaac ctcaacgatg
     3421 gctggacaga cacagctcat ggatccggca ttatccctat gaaggccgac ctcgagctgg
     3481 acttttctct gcccagctcc gcttcctaca catatagaag gcagctgcag aaccccgcta
     3541 atgagcaaga gaagatcccc ttccatctgc aaatcagcaa acaagtgatc cacgctgaga
     3601 ttcagcacct cggccactgg atggatgcca cctttaatct caagaccgcc ttccactgtt
     3661 atggcagctg cgagaagtac gcctaccctt ggcaaaccgc cggatgcttc gtcgaaaagg
     3721 attacgagta cgagaccggc tggggctgca atcctcccga ctgccccggc gtcggcaccg
     3781 gctgcacagc ttgcggagtc tatctggaca aactgaagtc cgtcggcaag gtgtttaaga
     3841 tcgtgtctct gaggtataca aggaaggtct gcattcagct cggcaccggc caaacatgca
     3901 agacagtgga cagcaacgac tgcctcatta caacctccgt caaggtgtgc ctcatcggca
     3961 ccatttccaa attccagccc tccgacacac tgctgtttct gggacctctg caacaaggcg
     4021 gactcatctt taagcagtgg tgcaccacaa catgccagtt tggagatccc ggcgacatta
     4081 tgagcacacc taccggcatg aagtgccccg agctgaacgg atcctttaga aagaagtgcg
     4141 cttttgctac cacacccgtg tgccagttcg atggcaatac catctccgga tacaagagga
     4201 tggtcgccac caaggattcc ttccagagct tcaatgtgac cgagccccat atcagcacaa
     4261 gcgctctgga atggatcgat ctggatagct ctctgaggga ccatatcaac gtcatcgtgt
     4321 ctagggatct gagctttcaa gatttgagcg agaccccttg ccaagtggat ctgaccacaa
     4381 gcgctacaga tggcgcttgg ggctccggag tgggatttaa tctggtctgc accgtgtctc
     4441 tgacagagtg ttccgctttc ctcaccagca tcaaagcttg ccatgccgcc atgtgctacg
     4501 gcagcaccac cacaaatctc gtgagaggac agaacaccat ccatgtcgtg ggaaagggcg
     4561 gacactccgg ctccaaattc atgtgttgcc acgacaccaa atgcagcagc accggactgg
     4621 tggctgctgc cccccatctg gacagagtga ccggcttcaa tcaagccgac agcgacaaga
     4681 ttttcgacga cggagccccc gagtgcggaa tgagctgctg gttcaaaaag ctgggcgagt
     4741 gggtgctcgg cgtgctcaac ggaaactgga tggtggtcgc cgtgctgatc gctctgctga
     4801 ttctgagcat ctttctcttc gctctgtgct gtcctaggag gcccagctac aagaaagacc
     4861 acaaaccttg atgtcttcct ggagctccag tcattgacta taacgtctca gatctttttc
     4921 cctctgccaa aaattatggg gacatcatga agccccttga gcatctgact tctggctaat
     4981 aaaggaaatt tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa
     5041 ggacatatgg gagggcaaat catttaaaac atcagaatga gtatttggtt tagagtttgg
     5101 caacatatgc ccatatgctg gctgccatga acaaaggttg gctataaaga ggtcatcagt
     5161 atatgaaaca gccccctgct gtccattcct tattccatag aaaagccttg acttgaggtt
     5221 agattttttt tatattttgt tttgtgttat ttttttcttt aacatcccta aaattttcct
     5281 tacatgtttt actagccaga tttttcctcc tctcctgact actcccagtc atagctgtcc
     5341 ctcttctctt atggagatcc ctcgacggat cggccgcaat tcgtaatcat gtcatagctg
     5401 tttcctgtgt ggaattgtga gcggataaca atttcacaca acatacgagc cggaagcata
     5461 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
     5521 ctgcccgctt tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc
     5581 gcggggagag gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg
     5641 cgctcggtcg ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta
     5701 tccacagaat caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc
     5761 aggaaccgta aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag
     5821 catcacaaaa atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac
     5881 caggcgtttc cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc
     5941 ggatacctgt ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt
     6001 aggtatctca gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc
     6061 gttcagcccg accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga
     6121 cacgacttat cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta
     6181 ggcggtgcta cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta
     6241 tttggtatct gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga
     6301 tccggcaaac aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg
     6361 cgcagaaaaa aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag
     6421 tggaacgaaa actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc
     6481 tagatccttt taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact
     6541 tggtctgaca gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt
     6601 cgttcatcca tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta
     6661 ccatctggcc ccagtgctgc aatgataccg cgagacccac gctcaccggc accagattta
     6721 tcagcaataa accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc
     6781 gcctccatcc agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat
     6841 agtttgcgca acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt
     6901 atggcttcat tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg
     6961 tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca
     7021 gtgttatcac tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta
     7081 agatgctttt ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg
     7141 cgaccgagtt gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact
     7201 ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg
     7261 ctgttgagat ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt
     7321 actttcacca gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga
     7381 ataagggcga cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc
     7441 atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa
     7501 caaatagggg ttccgcgcac atttccccga aaagtgccac ctaaattgta agcgttaata
     7561 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg
     7621 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc
     7681 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa
     7741 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt
     7801 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac
     7861 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta
     7921 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg
     7981 cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     8041 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     8101 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
     8161 gcgcgtaata cgactcacta tagggcgaat tggagctcca ccgcggtg
//
