LOCUS       sequence-407705-        8675 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of emiRFP2-T2A-mtq2  in yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Botman D, de Groot DH, Wehrens M, van Rossum K, Yska S, van der 
            Kieft B, Albert V, Minghetti N, Goedhart J, Teusink B
  TITLE     Side-by-side systematic characterization of novel FPs in budding 
            yeast
  JOURNAL   bioRxiv 2025.06.27.661975; doi: 
            https://doi.org/10.1101/2025.06.27.661975
REFERENCE   2  (bases 1 to 8675)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2025.06.27.661975; doi: https://doi.org/10.1101/2025.06.27.661975"
COMMENT     Sequence Label: pDRF1-GW emiRFP2-T2A-mtq2
FEATURES             Location/Qualifiers
     source          1..8675
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    800..824
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             1761..1814
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     regulatory      1830..1839
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1836..1838,1839..1841,1842..2555)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Goedhart et 
                     al., 2012)"
                     /label=mTurquoise2
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIK
                     ANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1881..1902)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2142..2161)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2489..2510
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(2565..2589)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(2651..2670)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2652..2670)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     terminator      2818..3005
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(2873..2897)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     primer_bind     complement(3017..3034)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3017..3033)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3026..3048)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3469..4815
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     CDS             complement(4926..5729)
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     promoter        complement(5730..5945)
                     /gene="S. cerevisiae URA3"
                     /label=URA3 promoter
     primer_bind     complement(5970..5988)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6056..6160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6161..6229,6230..7021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6379..6398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7192..7780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7681..7700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7934..7951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8068..8089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8104..8109,8110..8127,8128..8134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8142..8158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8147..8169
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8166..8182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8166..8182
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      complement(8212..8667)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8349..8370)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     8561..8580
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 caagcttcct gaaacggaga aacataaaca ggcattgctg ggatcaccca tacatcactc
       61 tgttttgcct gaccttttcc ggtaatttga aaacaaaccc ggtctcgaag cggagatccg
      121 gcgataatta ccgcagaaat aaacccatac acgagacgta gaaccagccg cacatggccg
      181 gagaaactcc tgcgagaatt tcgtaaactc gcgcgcattg catctgtatt tcctaatgcg
      241 gcacttccag gcctcgatcg agaccgttta tccattgctt ttttgttgtc tttttccctc
      301 gttcacagaa agtctgaaga agctatagta gaactatgag ctttttttgt ttctgttttc
      361 cttttttttt tttttacctc tgtggaaatt gttactctca cactctttag ttcgtttgtt
      421 tgttttgttt attccaatta tgaccggtga cgaaacgtgg tcgatggtgg gtaccgctta
      481 tgctcccctc cattagtttc gattatataa aaaggccaaa tattgtatta ttttcaaatg
      541 tcctatcatt atcgtctaac atctaatttc tcttaaattt tttctctttc tttcctataa
      601 caccaatagt gaaaatcttt ttttcttcta tatctacaaa aacttttttt ttctatcaac
      661 ctcgttgata aattttttct ttaacaatcg ttaataatta attaattgga aaataaccat
      721 tttttctctc ttttatacac acattcaaaa gaaagaaaaa aaatataccc cagcctcgac
      781 tagtgaattc gcggccatca caagtttgta caaaaaagca ggctgctagc atggccgagg
      841 gcagcgtggc cagacagccc gacctgctga cctgtgagca cgaagaaatc catctggccg
      901 gatccatcca gccacacgga acactgctgg tggtctccga gcctgaccat agaataattc
      961 aggcatctgc caacgccgct gaattcctga atctgggaag tgtgctggga atccccctgg
     1021 ctgagatcga cggggatcta ctgatcaaga ttctgcccca cctggacctt accgcagaag
     1081 gaatgccagt ggccgtcagg tgccgcattg gaaacccctc cacagagtac gacggactga
     1141 tgcatcgacc acctgaagga ggactgatca ttgagctgga acgggctggg ccatccattg
     1201 atctgagcgg aacactggct cccgcactgg agcgaattcg aactgcagga tccctgaggg
     1261 ctctgtgcga cgatactgta ctgctgttcc agcagtgtac cggctacgac cgagtgatgg
     1321 tctatcggtt cgatgaacgg gggcacggag aggtgtattc agaaattcat gtgactggac
     1381 tggagagcta ctttggcaac aggtatccta gctccctcgt gccacagatg gcccggcggc
     1441 tgtacgtgag acagagggtg cgcgtcctgg tagatgtcac ctatcagcct gtgccactgg
     1501 agccaagact gagtcccctg actggcaggg acctggatat gtcagggtgt tttctgcgct
     1561 ctatgagtcc tactcacctg cagttcctga agaacatggg cgtgcgggca acactggtgg
     1621 tcagcctggt ggtcggaggc aaactgtggg ggctggtgat ctgccaccat tatctgccac
     1681 gcttcatcca ttttgagctg cgagcaattt gtgtgctgct ggccgaagct atcgcaaccc
     1741 gcattacagc cctgtccgga gagggcagag gaagtcttct aacatgcggt gacgtggagg
     1801 agaatcccgg ccctggggat ccaccggtcg ccaccatggt gagcaagggc gaggagctgt
     1861 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1921 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1981 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg tcctggggcg
     2041 tgcagtgctt cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     2101 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     2161 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     2221 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tactttagcg
     2281 acaacgtcta tatcaccgcc gacaagcaga agaacggcat caaggccaac ttcaagatcc
     2341 gccacaacat cgaggacggc ggcgtgcagc tcgccgacca ctaccagcag aacaccccca
     2401 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccaagctga
     2461 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     2521 ggatcactct cggcatggac gagctgtaca agtaaagcgg ccgcacccag ctttcttgta
     2581 caaagtggtg tagttaattc atgatggccg ctgcaggtcg acctcgaggg ggggcccggt
     2641 acccaattcg ccctatagtg agtcgtatta cgcgcggatc cagctttgga cttcttcgcc
     2701 agaggtttgg tcaagtctcc aatcaaggtt gtcggcttgt ctaccttgcc agaaatttac
     2761 gaaaagatgg aaaagggtca aatcgttggt agatacgttg ttgacacttc taaataagcg
     2821 aatttcttat gatttatgat ttttattatt aaataagtta taaaaaaaat aagtgtatac
     2881 aaattttaaa gtgactctta ggttttaaaa cgaaaattct tattcttgag taactctttc
     2941 ctgtaggtca ggttgctttc tcaggtatag catgaggtcg ctcttattga ccacacctct
     3001 accggcatgc caattcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg
     3061 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag
     3121 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcctga
     3181 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataatc ggatcgtact
     3241 tgttacccat cattgaattt tgaacatccg aacctgggag ttttccctga aacagatagt
     3301 atatttgaac ctgtataata atatatagtc tagcgcttta cggaagacaa tgtatgtatt
     3361 tcggttcctg gagaaactat tgcatctatt gcataggtaa tcttgcacgt cgcatccccg
     3421 gttcattttc tgcgtttcca tcttgcactt caatagcata tctttgttaa cgaagcatct
     3481 gtgcttcatt ttgtagaaca aaaatgcaac gcgagagcgc taatttttca aacaaagaat
     3541 ctgagctgca tttttacaga acagaaatgc aacgcgaaag cgctatttta ccaacgaaga
     3601 atctgtgctt catttttgta aaacaaaaat gcaacgcgag agcgctaatt tttcaaacaa
     3661 agaatctgag ctgcattttt acagaacaga aatgcaacgc gagagcgcta ttttaccaac
     3721 aaagaatcta tacttctttt ttgttctaca aaaatgcatc ccgagagcgc tatttttcta
     3781 acaaagcatc ttagattact ttttttctcc tttgtgcgct ctataatgca gtctcttgat
     3841 aactttttgc actgtaggtc cgttaaggtt agaagaaggc tactttggtg tctattttct
     3901 cttccataaa aaaagcctga ctccacttcc cgcgtttact gattactagc gaagctgcgg
     3961 gtgcattttt tcaagataaa ggcatccccg attatattct ataccgatgt ggattgcgca
     4021 tactttgtga acagaaagtg atagcgttga tgattcttca ttggtcagaa aattatgaac
     4081 ggtttcttct attttgtctc tatatactac gtataggaaa tgtttacatt ttcgtattgt
     4141 tttcgattca ctctatgaat agttcttact acaatttttt tgtctaaaga gtaatactag
     4201 agataaacat aaaaaatgta gaggtcgagt ttagatgcaa gttcaaggag cgaaaggtgg
     4261 atgggtaggt tatataggga tatagcacag agatatatag caaagagata cttttgagca
     4321 atgtttgtgg aagcggtatt cgcaatattt tagtagctcg ttacagtccg gtgcgttttt
     4381 ggttttttga aagtgcgtct tcagagcgct tttggttttc aaaagcgctc tgaagttcct
     4441 atactttcta gctagagaat aggaacttcg gaataggaac ttcaaagcgt ttccgaaaac
     4501 gagcgcttcc gaaaatgcaa cgcgagctgc gcacatacag ctcactgttc acgtcgcacc
     4561 tatatctgcg tgttgcctgt atatatatat acatgagaag aacggcatag tgcgtgttta
     4621 tgcttaaatg cgtacttata tgcgtctatt tatgtaggat gaaaggtagt ctagtacctc
     4681 ctgtgatatt atcccattcc atgcggggta tcgtatgctt ccttcagcac taccctttag
     4741 ctgttctata tgctgccact cctcaattgg attagtctca tccttcaatg ctatcatttc
     4801 ctttgatatt ggatcgatcc gatgataagc tgtcaaacat gagaattggg taataactga
     4861 tataattaaa ttgaagctct aatttgtgag tttagtatac atgcatttac ttataataca
     4921 gttttttagt tttgctggcc gcatcttctc aaatatgctt cccagcctgc ttttctgtaa
     4981 cgttcaccct ctaccttagc atcccttccc tttgcaaata gtcctcttcc aacaataata
     5041 atgtcagatc ctgtagagac cacatcatcc acggttctat actgttgacc caatgcgtct
     5101 cccttgtcat ctaaacccac accgggtgtc ataatcaacc aatcgtaacc ttcatctctt
     5161 ccacccatgt ctctttgagc aataaagccg ataacaaaat ctttgtcgct cttcgcaatg
     5221 tcaacagtac ccttagtata ttctccagta gatagggagc ccttgcatga caattctgct
     5281 aacatcaaaa ggcctctagg ttcctttgtt acttcttctg ccgcctgctt caaaccgcta
     5341 acaatacctg ggcccaccac accgtgtgca ttcgtaatgt ctgcccattc tgctattctg
     5401 tatacacccg cagagtactg caatttgact gtattaccaa tgtcagcaaa ttttctgtct
     5461 tcgaagagta aaaaattgta cttggcggat aatgccttta gcggcttaac tgtgccctcc
     5521 atggaaaaat cagtcaagat atccacatgt gtttttagta aacaaatttt gggacctaat
     5581 gcttcaacta actccagtaa ttccttggtg gtacgaacat ccaatgaagc acacaagttt
     5641 gtttgctttt cgtgcatgat attaaatagc ttggcagcaa caggactagg atgagtagca
     5701 gcacgttcct tatatgtagc tttcgacatg atttatcttc gtttcctgca tgtttttgtt
     5761 ctgtgcagtt gggttaagaa tactgggcaa tttcatgttt cttcaacact acatatgcgt
     5821 atatatacca atctaagtct gtgctccttc cttcgttctt ccttctgttc ggagattacc
     5881 gaatcaaaaa aatttcaagg aaaccgaaat caaaaaaaag aataaaaaaa aaatgatgaa
     5941 ttgaaaagct aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
     6001 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
     6061 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     6121 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     6181 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     6241 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     6301 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     6361 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     6421 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     6481 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     6541 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     6601 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     6661 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     6721 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     6781 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     6841 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     6901 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     6961 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     7021 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     7081 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     7141 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     7201 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     7261 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     7321 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     7381 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     7441 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     7501 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     7561 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     7621 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     7681 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     7741 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     7801 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     7861 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     7921 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     7981 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     8041 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     8101 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     8161 tcacacagga aacagctatg accatgatta cgccaagctt accgcatcag gaaattgtaa
     8221 gcgttaatat tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     8281 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     8341 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     8401 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     8461 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     8521 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     8581 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     8641 cgcttaatgc gccgctacag ggcgcgtcca ttcgc
//
