LOCUS       sequence-407731-        9527 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human VEGFR1 receptor tyrosine kinase in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9527)
  AUTHORS   Gudernova I, Foldynova-Trantirkova S, Ghannamova BE, Fafilek B, 
            Varecha M, Balek L, Hruba E, Jonatova L, Jelinkova I, Kunova 
            Bosakova M, Trantirek L, Mayer J, Krejci P
  TITLE     One reporter for in-cell activity profiling of majority of protein 
            kinase oncogenes.
  JOURNAL   Elife. 2017 Feb 15;6. pii: e21536. doi: 10.7554/eLife.21536.
  PUBMED    28199182
REFERENCE   2  (bases 1 to 9527)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2017-02-15"; volume: "6. pii: e21536. doi"; pages: " 
            10.7554/eLife.21536"
COMMENT     Sequence Label: pcDNA3.1-VEGFR1-V5/HIS
FEATURES             Location/Qualifiers
     source          1..9527
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     27..48
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(167..187)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        172..501
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      352..487
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     414..433
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             568..1362
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(622..641)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1232..1251
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1538..1659
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1575..1594)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1629..1648
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1708..1724)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1708..1724)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1721..1743)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1732..1748
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1756..1762,1763..1780,1781..1786))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1801..1822
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1939..1956)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2110..2698)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2190..2209)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2869..3660,3661..3729))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3492..3511
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3730..3834)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3909..3928)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4100..4479
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4480..4683
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4634..4654
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4728..4747
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4728..4746
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8890..8931
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             8941..8958
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(8981..8998)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8987..9211
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      join(9257..9527,1..158)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9344..9363)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa
       61 ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt
      121 aaaaaatgag ctgatttaac aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag
      181 ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
      241 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
      301 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
      361 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
      421 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
      481 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cggatctgat
      541 caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca cgcaggttct
      601 ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac aatcggctgc
      661 tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt tgtcaagacc
      721 gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc gtggctggcc
      781 acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg aagggactgg
      841 ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag
      901 aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc
      961 ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat ggaagccggt
     1021 cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc cgaactgttc
     1081 gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca tggcgatgcc
     1141 tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga ctgtggccgg
     1201 ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat tgctgaagag
     1261 cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg
     1321 cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact ctggggttcg
     1381 cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc
     1441 cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca
     1501 gcgcggggat ctcatgctgg agttcttcgc ccaccccaac ttgtttattg cagcttataa
     1561 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     1621 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgta taccgtcgac
     1681 ctctagctag agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc
     1741 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct ggggtgccta
     1801 atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc agtcgggaaa
     1861 cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat
     1921 tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     1981 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     2041 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     2101 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     2161 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     2221 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     2281 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     2341 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     2401 atccgataac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     2461 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     2521 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
     2581 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     2641 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     2701 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     2761 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg
     2821 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     2881 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     2941 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     3001 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     3061 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     3121 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     3181 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     3241 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     3301 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     3361 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     3421 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     3481 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     3541 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     3601 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     3661 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     3721 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     3781 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     3841 tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc cctatggtgc
     3901 actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct ccctgcttgt
     3961 gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag gcaaggcttg
     4021 accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct tcgcgatgta
     4081 cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta atcaattacg
     4141 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
     4201 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     4261 atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact
     4321 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     4381 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     4441 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     4501 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     4561 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     4621 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     4681 gctctctggc taactagaga acccactgct tactggctta tcgaaattaa tacgactcac
     4741 tatagggaga cccaagctgg ctagttaagc ttggtaccga gctcggatcc agtacccttc
     4801 accatggtca gctactggga caccggggtc ctgctgtgcg cgctgctcag ctgtctgctt
     4861 ctcacaggat ctagttcagg ttcaaaatta aaagatcctg aactgagttt aaaaggcacc
     4921 cagcacatca tgcaagcagg ccagacactg catctccaat gcagggggga agcagcccat
     4981 aaatggtctt tgcctgaaat ggtgagtaag gaaagcgaaa ggctgagcat aactaaatct
     5041 gcctgtggaa gaaatggcaa acaattctgc agtactttaa ccttgaacac agctcaagca
     5101 aaccacactg gcttctacag ctgcaaatat ctagctgtac ctacttcaaa gaagaaggaa
     5161 acagaatctg caatctatat atttattagt gatacaggta gacctttcgt agagatgtac
     5221 agtgaaatcc ccgaaattat acacatgact gaaggaaggg agctcgtcat tccctgccgg
     5281 gttacgtcac ctaacatcac tgttacttta aaaaagtttc cacttgacac tttgatccct
     5341 gatggaaaac gcataatctg ggacagtaga aagggcttca tcatatcaaa tgcaacgtac
     5401 aaagaaatag ggcttctgac ctgtgaagca acagtcaatg ggcatttgta taagacaaac
     5461 tatctcacac atcgacaaac caatacaatc atagatgtcc aaataagcac accacgccca
     5521 gtcaaattac ttagaggcca tactcttgtt ctcaattgta ctgctaccac tcccttgaac
     5581 acgagagttc aaatgacctg gagttaccct gatgaaaaaa ataagagagc ttccgtaagg
     5641 cgacgaattg accaaagcaa ttcccatgcc aacatattct acagtgttct tactattgac
     5701 aaaatgcaga acaaagacaa aggactttat acttgtcgtg taaggagtgg accatcattc
     5761 aaatctgtta acacctcagt gcatatatat gataaagcat tcatcactgt gaaacatcga
     5821 aaacagcagg tgcttgaaac cgtagctggc aagcggtctt accggctctc tatgaaagtg
     5881 aaggcatttc cctcgccgga agttgtatgg ttaaaagatg ggttacctgc gactgagaaa
     5941 tctgctcgct atttgactcg tggctactcg ttaattatca aggacgtaac tgaagaggat
     6001 gcagggaatt atacaatctt gctgagcata aaacagtcaa atgtgtttaa aaacctcact
     6061 gccactctaa ttgtcaatgt gaaaccccag atttacgaaa aggctgtgtc atcgtttcca
     6121 gacccggctc tctacccact gggcagcaga caaatcctga cttgtaccgc atatggtatc
     6181 cctcaaccta caatcaagtg gttctggcac ccctgtaacc ataatcattc cgaagcaagg
     6241 tgtgactttt gttccaataa tgaagagtcc tttatcctgg atgctgacag caacatggga
     6301 aacagaattg agagcatcac tcagcgcatg gcaataatag aaggaaagaa taagatggct
     6361 agcaccttgg ttgtggctga ctctagaatt tctggaatct acatttgcat agcttccaat
     6421 aaagttggga ctgtgggaag aaacataagc ttttatatca cagatgtgcc aaatgggttt
     6481 catgttaact tggaaaaaat gccgacggaa ggagaggacc tgaaactgtc ttgcacagtt
     6541 aacaagttct tatacagaga cgttacttgg attttactgc ggacagttaa taacagaaca
     6601 atgcactaca gtattagcaa gcaaaaaatg gccatcacta aggagcactc catcactctt
     6661 aatcttacca tcatgaatgt ttccctgcaa gattcaggca cctatgcctg cagagccagg
     6721 aatgtataca caggggaaga aatcctccag aagaaagaaa ttacaatcag agatcaggaa
     6781 gcaccatacc tcctgcgaaa cctcagtgat cacacagtgg ccatcagcag ttccaccact
     6841 ttagactgtc atgctaatgg tgtccccgag cctcagatca cttggtttaa aaacaaccac
     6901 aaaatacaac aagagcctgg aattatttta ggaccaggaa gcagcacgct gtttattgaa
     6961 agagtcacag aagaggatga aggtgtctat cactgcaaag ccaccaacca gaagggctct
     7021 gtggaaagtt cagcatacct cactgttcaa ggaacctcgg acaagagtaa tctggagctg
     7081 atcactctaa catgcacctg tgtggctgcg actctcttct ggctcctatt aaccctcttt
     7141 atccgaaaaa tgaaaaggtc ttcttctgaa ataaagactg actacctatc aattataatg
     7201 gacccagatg aagttccttt ggatgagcag tgtgagcggc tcccttatga tgccagcaag
     7261 tgggagtttg cccgggagag acttaaactg ggcaaatcac ttggaagagg ggcttttgga
     7321 aaagtggttc aagcatcagc atttggcatt aagaaatcac ctacgtgccg gactgtggct
     7381 gtgaaaatgc tgaaagaggg ggccacggcc agcgagtaca aagctctgat gactgagcta
     7441 aaaatcttga cccacattgg ccaccatctg aacgtggtta acctgctggg agcctgcacc
     7501 aagcaaggag ggcctctgat ggtgattgtt gaatactgca aatatggaaa tctctccaac
     7561 tacctcaaga gcaaacgtga cttatttttt ctcaacaagg atgcagcact acacatggag
     7621 cctaagaaag aaaaaatgga gccaggcctg gaacaaggca agaaaccaag actagatagc
     7681 gtcaccagca gcgaaagctt tgcgagctcc ggctttcagg aagataaaag tctgagtgat
     7741 gttgaggaag aggaggattc tgacggtttc tacaaggagc ccatcactat ggaagatctg
     7801 atttcttaca gttttcaagt ggccagaggc atggagttcc tgtcttccag aaagtgcatt
     7861 catcgggacc tggcagcgag aaacattctt ttatctgaga acaacgtggt gaagatttgt
     7921 gattttggcc ttgcccggga tatttataag aaccccgatt atgtgagaaa aggagatact
     7981 cgacttcctc tgaaatggat ggctcctgaa tctatctttg acaaaatcta cagcaccaag
     8041 agcgacgtgt ggtcttacgg agtattgctg tgggaaatct tctccttagg tgggtctcca
     8101 tacccaggag tacaaatgga tgaggacttt tgcagtcgcc tgagggaagg catgaggatg
     8161 agagctcctg agtactctac tcctgaaatc tatcagatca tgctggactg ctggcacaga
     8221 gacccaaaag aaaggccaag atttgcagaa cttgtggaaa aactaggtga tttgcttcaa
     8281 gcaaatgtac aacaggatgg taaagactac atcccaatca atgccatact gacaggaaat
     8341 agtgggttta catactcaac tcctgccttc tctgaggact tcttcaagga aagtatttca
     8401 gctccgaagt ttaattcagg aagctctgat gatgtcagat acgtaaatgc tttcaagttc
     8461 atgagcctgg aaagaatcaa aacctttgaa gaacttttac cgaatgccac ctccatgttt
     8521 gatgactacc agggcgacag cagcactctg ttggcctctc ccatgctgaa gcgcttcacc
     8581 tggactgaca gcaaacccaa ggcctcgctc aagattgact tgagagtaac cagtaaaagt
     8641 aaggagtcgg ggctgtctga tgtcagcagg cccagtttct gccattccag ctgtgggcac
     8701 gtcagcgaag gcaagcgcag gttcacctac gaccacgctg agctggaaag gaaaatcgcg
     8761 tgctgctccc cgcccccaga ctacaactcg gtggtcctgt actccacccc acccatcaag
     8821 ggtcaagaca attctgcaga tatccagcac agtggcggcc gctcgagtct agagggcccg
     8881 cggttcgaag gtaagcctat ccctaaccct ctcctcggtc tcgattctac gcgtaccggt
     8941 catcatcacc atcaccattg agtttaaacc cgctgatcag cctcgactgt gccttctagt
     9001 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
     9061 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
     9121 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
     9181 aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac cagctggggc
     9241 tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     9301 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     9361 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     9421 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     9481 ggttcacgta gtgggccatc gccctgatag acggtttttc gcccttt
//
