LOCUS       sequence-407735-       11165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BCR-ABL oncogene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11165)
  AUTHORS   Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky 
            NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek 
            L, Krejci P
  TITLE     Elucidation of protein interactions necessary for the maintenance of
            the BCR-ABL signaling complex.
  JOURNAL   Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. doi: 
            10.1007/s00018-019-03397-7. Epub 2019 Dec 9.
  PUBMED    31820037
REFERENCE   2  (bases 1 to 11165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-019-03397-7"; journalName: "Cell Mol Life Sci"; 
            date: "2020-10"; volume: "77"; issue: "19"; pages: "3885-3903"
COMMENT     Sequence Label: pcDNA3.1-BCR-ABL-p210-FLAG
FEATURES             Location/Qualifiers
     source          1..11165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(3369..3392)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3435..3454)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3436..3454)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3499..3702)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3528..3548)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3703..4082
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(4094..4549)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4231..4252)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4443..4462
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4576..4680
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4681..4749,4750..5541)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4899..4918)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        5601..5958
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5601..5792
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5624..5644)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5809..5944
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5993..6787
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5993..6787
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6047..6066)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6657..6676
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6975..6994)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    7019..7066
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7395..7983
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7884..7903
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    8115..8339
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8328..8345
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        8365..8383
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8365..8382
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gctggataat ggagcgtggt gatgagcccg tcggccaccg ttgaatgatg atgaaccaac
       61 tcggccaggg tgttgaagcg gctctcggag gagacgtaga gcttgccatc agaagcagtg
      121 ttgatcctgt aatggtacac cctcccttcg tatctcagcg agatggacct ctggccagga
      181 ctgctctcac tctcacgcac caagaagctg ccattgatcc cgctgctcag cagatactca
      241 gcggcattgc gggacacagg cccatggtac caggagtgtt tctccagact gttgactggc
      301 gtgatgtagt tgcttgggac ccagccttgg ccatttttgg tttgggcttc acaccattcc
      361 ccattgtgat tatagcctaa gacccggagc ttttcacctt tagttatgct tagagtgtta
      421 tctccactgg ccacaaaatc atacagtgca acgaaaaggt tggggtcatt ttcactgggt
      481 ccagcgagaa ggttttcctt ggagttccaa cgagcggctt cactcagacc ctgaggctca
      541 aagtcagatg ctactggccg ctgaagggct tttgaactct gcttaaatcc agtggctgag
      601 tggacgatga cattcagaaa cccatagagc cccggagact catcatcttc cttattgatg
      661 gtcagcggaa tgctgtggac agtctggagt ttcacacacg agttggtcag catctgcagc
      721 tccacggatg tcagggagaa gcttctgaaa cacttcttct gctgctcccg gatgttctcc
      781 ctccactctg cacgctcata gtcagaggag atcaggaacg tgtaactctt gccgttgcgg
      841 ctgtgcaccc tgaaggccat gctgggagac ataagcagca gcagtgactc ctgctccgac
      901 agcttcttct tcagcctctc cgtagccttg ctgcccttgt tcgccctctt ctctctctgg
      961 atgtcactct tgatctggga gatcttgatc ttcaaagcgt ccagctcctc atcgggcacc
     1021 agggggatgt tgggcactgc ctccagttca tccaccatct ggaagctgag atccgtgagc
     1081 ggaatgtacc atttgcagtc atactgctgc gttttgcctc cgctctgctt cttgagcttg
     1141 gtgcagagaa gcaggtcggt gaacaggaag acgtggcgca gcttgcgggc cccctccacc
     1201 agctccacca tgaagctgtc cttcagcagc tgccggtgct ctcccttctt caccgtcatg
     1261 gactgccgtc ggggtgtgat ctcctcattg atgctggaca ggaagttctg tgagatgcgg
     1321 agggcgtcct gcagcaaggg gtggtcaggg tggctggcag gagtgtgctt cagcaagtca
     1381 tggaggacca gcgtgctcct cgtcacacgg tccacaggct tgtagagcag agtttccaga
     1441 gagttcttgg tcgttggatc cttggcatct ttgttgcttc tggctctcag gttctcggag
     1501 atttctgcaa actgagcatt ggcctgacag cacttctcag ccatttccat ggcaactccg
     1561 tagttgtcca cgaaggcccg gtacacaccc agctggctgg ccagcttctg gaagaggtcg
     1621 cccacccgct gctggtggct ccactgctgc acgcggggga agagcccatc atagaactcc
     1681 ttgtggatct cgtagagctc aggcactttg aagaagatgg tctcgatctg ctgactcgtc
     1741 agcaccggct gagaggtggt ggcagcggct ttcaaaggct tcatgggcag cagcagtgcc
     1801 tccaggtggc tcaggtaagt ctcctcgcta gccaggattc ccgacaggac ccattttctc
     1861 atctccaagc ccttttccaa gtccagctca ctcgctttag tggactccag ggctcccgag
     1921 accagcgcat cccggctgcc cctgcccttg ctgctgaggt ggggcgatga ggagggcgag
     1981 tcatcaatgt agggcagccc atcttggtgc cggcgctgct cctctgcccg gtctgcagcg
     2041 cagccgtatc caggtggtgt tccgaacgag ccatctgcgt ctccatggaa ggcgccctcg
     2101 ccatcgttgg gccagatctg cccggtcttg cggacgccca cgatggtggc ctcggacacg
     2161 acaaccgggc agtgccggtg ccgcttatgg cactgcggcg tggggggact gctgctgtcg
     2221 aaggactgtt gcgagttctg cgagggagag cggcttttgt cccggaacat gcggtaggtg
     2281 gtggggcttg gggacacgcg gctggactgg ccagaggaga agtcctcctc gctggaggtg
     2341 aggttctcat tggagctgca gtccggggta tagccgcctc cgcaatcctc aaaactccgg
     2401 ggggagtagg acctgcgggg ccaggtaagg cgcttctcct gctcagaggt gctctggctg
     2461 cgcaggagcg ggcccttgcc ctccccttcc atcatgcccc cgacgtagat gctctggtag
     2521 ggctggtact ccaggggcgg ccaagggggc ctgctaccgc cattggcgtc gatcaggttg
     2581 tccttcagga agcgggggtt caactcggcg tcctcgtagt cgccgtcgac gccgcagctg
     2641 ctctccgagg agcgtccccg gtaagggggc ctggatgcat cccccacgct cgagcccgcg
     2701 ccgtgctggg actttttgcg ctccatctgc atggcctggc tgcccaggga gctgatgcgg
     2761 tccgacacct ctttgtcgtt gaccttcacc aggccgcgct cgtggtgaaa ctcgacgttc
     2821 acgtagaagg gcttctcggc gtccgccccg ggctggccat ggcccttgcg tatccgctcg
     2881 aagttggacc tgagcgccgc cacgctggcg gggggtcccc ggtcgtcccg ttcccccgac
     2941 gcggctgccc cgggcctgcg ggcggtcccg ggcctggcct tacccggaga accctcgccg
     3001 tcgggccggg cctcgggctc ctcggcgggc ggcgggtcgg ctccgtcggc gggcgctggc
     3061 tgcgggcgcg acgcggacgc tcggggctcg gaggcgccgt cgggggcctg cgccgcgcgc
     3121 cggaagcccc atcgctgccg gtcatagctc ttcttttcct tggccagcaa cgtctgcagg
     3181 tagatcatgc ggaagcgctc ctggttcacc tcctgctcca ggcgccgaat ggaggccttg
     3241 cagcgctcca gctcctgctc gatgtcgccc actgagcgca gctccatgcg cgggggctct
     3301 gagtccggga actgcgcctt ccacgcctcc gcgaagccca ccgggtccac cagatatctg
     3361 cagaattctt tatcgtcatc gtctttgtaa tccatgtttt gattcccggg ggatccggta
     3421 ccaagcttgg gtctccctat agtgagtcgt attaatttcg ataagccagt aagcagtggg
     3481 ttctctagtt agccagagag ctctgcttat atagacctcc caccgtacac gcctaccgcc
     3541 catttgcgtc aatggggcgg agttgttacg acattttgga aagtcccgtt gattttggtg
     3601 ccaaaacaaa ctcccattga cgtcaatggg gtggagactt ggaaatcccc gtgagtcaaa
     3661 ccgctatcca cgcccattga tgtactgcca aaaccgcatc accatggtaa tagcgatgac
     3721 taatacgtag atgtactgcc aagtaggaaa gtcccataag gtcatgtact gggcataatg
     3781 ccaggcgggc catttaccgt cattgacgtc aatagggggc gtacttggca tatgatacac
     3841 ttgatgtact gccaagtggg cagtttaccg taaatactcc acccattgac gtcaatggaa
     3901 agtccctatt ggcgttacta tgggaacata cgtcattatt gacgtcaatg ggcgggggtc
     3961 gttgggcggt cagccaggcg ggccatttac cgtaagttat gtaacgcgga actccatata
     4021 tgggctatga actaatgacc ccgtaattga ttactattaa taactagtca ataatcaatg
     4081 tcaacgcgcg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
     4141 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     4201 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     4261 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     4321 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     4381 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     4441 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     4501 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc aggtggcact
     4561 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     4621 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     4681 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     4741 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     4801 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     4861 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     4921 cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     4981 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     5041 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     5101 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
     5161 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
     5221 cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
     5281 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
     5341 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
     5401 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
     5461 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
     5521 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
     5581 ttattgaaaa aggaagagtc ctgaggcgga aagaaccagc tgtggaatgt gtgtcagtta
     5641 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     5701 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     5761 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     5821 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     5881 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     5941 ggcctaggct tttgcaaaga tcgatcaaga gacaggatga ggatcgtttc gcatgattga
     6001 acaagatgga ttgcacgcag gttctccggc cgcttgggtg gagaggctat tcggctatga
     6061 ctgggcacaa cagacaatcg gctgctctga tgccgccgtg ttccggctgt cagcgcaggg
     6121 gcgcccggtt ctttttgtca agaccgacct gtccggtgcc ctgaatgaac tgcaagacga
     6181 ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct tgcgcagctg tgctcgacgt
     6241 tgtcactgaa gcgggaaggg actggctgct attgggcgaa gtgccggggc aggatctcct
     6301 gtcatctcac cttgctcctg ccgagaaagt atccatcatg gctgatgcaa tgcggcggct
     6361 gcatacgctt gatccggcta cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg
     6421 agcacgtact cggatggaag ccggtcttgt cgatcaggat gatctggacg aagagcatca
     6481 ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg agcatgcccg acggcgagga
     6541 tctcgtcgtg acccatggcg atgcctgctt gccgaatatc atggtggaaa atggccgctt
     6601 ttctggattc atcgactgtg gccggctggg tgtggcggac cgctatcagg acatagcgtt
     6661 ggctacccgt gatattgctg aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct
     6721 ttacggtatc gccgctcccg attcgcagcg catcgccttc tatcgccttc ttgacgagtt
     6781 cttctgagcg ggactctggg gttcgaaatg accgaccaag cgacgcccaa cctgccatca
     6841 cgagatttcg attccaccgc cgccttctat gaaaggttgg gcttcggaat cgttttccgg
     6901 gacgccggct ggatgatcct ccagcgcggg gatctcatgc tggagttctt cgcccaccct
     6961 agggggaggc taactgaaac acggaaggag acaataccgg aaggaacccg cgctatgacg
     7021 gcaataaaaa gacagaataa aacgcacggt gttgggtcgt ttgttcataa acgcggggtt
     7081 cggtcccagg gctggcactc tgtcgatacc ccaccgagac cccattgggg ccaatacgcc
     7141 cgcgtttctt ccttttcccc accccacccc ccaagttcgg gtgaaggccc agggctcgca
     7201 gccaacgtcg gggcggcagg ccctgccata gcctcaggtt actcatatat actttagatt
     7261 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     7321 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     7381 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
     7441 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
     7501 aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag
     7561 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
     7621 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
     7681 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
     7741 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
     7801 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
     7861 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
     7921 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
     7981 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
     8041 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc cactggttct
     8101 ttccgcctca gaagccatag agcccaccgc atccccagca tgcctgctat tgtcttccca
     8161 atcctccccc ttgctgtcct gccccacccc accccccaga atagaatgac acctactcag
     8221 acaatgcgat gcaatttcct cattttatta ggaaaggaca gtgggagtgg caccttccag
     8281 ggtcaaggaa ggcacggggg aggggcaaac aacagatggc tggcaactag aaggcacagt
     8341 cgaggctgat cagcgagctc tagcatttag gtgacactat agaatagggc cctctagact
     8401 agtcatctac tcgagcggcc gcctacctct gcactatgtc actgatttcc ttcaccgaac
     8461 tgaggagctt gctgaagtcc tgagtggccg ctggaccact gcctgctgtc gccgggcaga
     8521 tctgaagctc ccggagatta ttctccagtt tgttgatggc ctctcggaag gcaaacttgt
     8581 tcctcatttg ctggatggaa tccacatagc tcacgcagaa cgtgtagagg tttttgccgg
     8641 cctccagcac tgcgctgtgg ctggccatct gctcggagtt cctagagatg gcgaggcaca
     8701 gcgcctcggt gctgtccagg accacgccct tggtgatggc gccgctggcg atccgctctg
     8761 gaggctggcg ggttttccga agagacactc gggttgatat gagagggatg aaggcggtgg
     8821 aagacggctg gtcccctgcc agggccgagg atgctgatgg caacgtggag ggaacggggg
     8881 ctgggctgat gggggtcccc gacggcttgg cggactgtgg ctttggagtg gccgggagca
     8941 cgggcttttt gaggccctct cccggctggc tgggcttggc agcgtcactg ttcacagcat
     9001 caaccagact cgtggctttt gtctttgcgc ccaggactgc ctccccggcc gcctcctggc
     9061 tcgggctctg cgagggcttt cctccagcct tccctgcaga ggctgctggt gggggcggcg
     9121 gggcaggttt gagcctggac aatttcccct tgtccctccc tggcgactca gaggagtgct
     9181 tgtgcctcct cactctggac tcctcggcgg ggcccttgct ggtgccccct ggtgcacctg
     9241 agcctgcttt gctggtgggg gtcactggct cagctgcagc aggggtccct aaggcactgc
     9301 cctttccagc ttcttccttg tgggggaggc ccgaggcagg ggccacggtg acctgccgcc
     9361 ggaggggttt tggagtcagg ttgggcgggc tggagcccgg gctggactcc atgatgtctt
     9421 tgaagacctc atcagcagct tcctcattct ttttcaccag cctgggggga ggcgttactg
     9481 tgcctcgggt cacctggtca gacctgttct cccctgccct cttccgaggc agagccggct
     9541 tctcactttt gtgccctcca aatgtggacg agtcaaactg tcttcccgtg gactgcaagt
     9601 cccgaggcag cgtgactgac ctccactccg tgtccttggc cccatgggga acgcaggagg
     9661 cggagcaaga gcgcaggaag cgcttgctgg agctgccacc gccctcctcc tcgccggtgg
     9721 ctaggcggct gctggtcagc gtgctggact tcttccacag gtggggagac cggaagcctg
     9781 agcccccaga ctcccggagg gctccattgg ggaccccagc cccattgctg ggctttgggg
     9841 acttggctgg gtcagctgtg tccaaggggg tgaaagccag tgccccgttg ctgatgtctc
     9901 ggccctcttc ctcgccggcc cctctgcgct ccggctggcc gtccatctcc cggaaggagc
     9961 tgctgcgttt gggaggggtt ggggctgtct tcttcttctt cttgatcaag gcgctgaaca
    10021 agttggtctt tttgtctttg gggagaaggc gctcatcttc attcaggccg ccctccgggg
    10081 gacctcgctc ttttcgaggg agcaatggag acacggcagg ctcatggtcc agaggatcgc
    10141 tctctccctg gcccttggag tgaggcatct caggcacgtc agtggtgtct ctgtgctctg
    10201 cagctctcct ggaggtcctc gtcttggtgg gcagctctgg ggcctgcagc aaggtactca
    10261 cagccccacg gacgccttgt ttccccagct ccttttccac ttcgtctgag atactggatt
    10321 cctggaacat tgtttcaaag gcttggtgga tttcagcaaa ggagggccgg tcagagggat
    10381 tccactgcca acatgctcgc atgagttcat agaccttctc tgggcagcct tctgggcgct
    10441 ccatgcggta gtccttctct agcagctcat acacctggga caggtcaatt cccgggtaag
    10501 gggacatgcc ataggtagca atttcccaaa gcaatactcc aaatgcccag acgtcggact
    10561 tgatggagaa cttgttgtag gccaggctct cgggtgcagt ccatttgatg gggaacttgg
    10621 ctccagcatg ggctgtgtag gtgtcccctg tcatcaacct gctcaggcca aaatcagcta
    10681 ccttcaccaa gtggttctcc cctaccaggc agtttcgggc agcaagatct ctgtggatga
    10741 agtttttctt ctccaggtac tccatggctg acgagatctg agtggccatg tacagcagca
    10801 ccacggcgtt cacctcctgc cggttgcact ccctcaggta gtccaggagg ttcccgtagg
    10861 tcatgaactc agtgatgata tagaacgggg gctcccgggt gcagacccca aggagctgca
    10921 ccaggttagg gtgtttgatc tctttcatga ctgcagcttc tttcaagaac tcttccacct
    10981 ccatggtgtc ctccttcaag gtcttcacgg ccaccgtcag gctgtatttc ttccacacgc
    11041 cctcgtacac ctccccgtac tggcccccgc ccagcttgtg cttcatggtg atgtccgtgc
    11101 gttccatctc ccacttgtcg tagttggggg acacaccata gacagtgggc ttgttgcgct
    11161 ttggg
//
