LOCUS       sequence-407736-       10604 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BCR-ABL oncogene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10604)
  AUTHORS   Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky 
            NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek 
            L, Krejci P
  TITLE     Elucidation of protein interactions necessary for the maintenance of
            the BCR-ABL signaling complex.
  JOURNAL   Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. doi: 
            10.1007/s00018-019-03397-7. Epub 2019 Dec 9.
  PUBMED    31820037
REFERENCE   2  (bases 1 to 10604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-019-03397-7"; journalName: "Cell Mol Life Sci"; 
            date: "2020-10"; volume: "77"; issue: "19"; pages: "3885-3903"
COMMENT     Sequence Label: pcDNA3.1-BCR-ABL-p210-Delta-AB-FLAG
FEATURES             Location/Qualifiers
     source          1..10604
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(4158..4176)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4159..4176)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4196..4213)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4202..4426
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4558..5146)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4638..4657)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5475..5522
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5547..5566
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5754..6548)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5754..6548)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5865..5884)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6475..6494
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6583..6940)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6597..6732
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6749..6940
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6897..6917
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7000..7791,7792..7860))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7623..7642
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7861..7965)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7992..8447
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8079..8098)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8289..8310
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        8459..8838
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8839..9042
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8993..9013
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9087..9106
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9087..9105
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9149..9172
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgctgaagca cactcctgcc agccaccctg accacccctt gctgcaggac gccctccgca
       61 tctcacagaa cttcctgtcc agcatcaatg aggagatcac accccgacgg cagtccatga
      121 cggtgaagaa gggagagcac cggcagctgc tgaaggacag cttcatggtg gagctggtgg
      181 agggggcccg caagctgcgc cacgtcttcc tgttcaccga cctgcttctc tgcaccaagc
      241 tcaagaagca gagcggaggc aaaacgcagc agtatgactg caaatggtac attccgctca
      301 cggatctcag cttccagatg gtggatgaac tggaggcagt gcccaacatc cccctggtgc
      361 ccgatgagga gctggacgct ttgaagatca agatctccca gatcaagagt gacatccaga
      421 gagagaagag ggcgaacaag ggcagcaagg ctacggagag gctgaagaag aagctgtcgg
      481 agcaggagtc actgctgctg cttatgtctc ccagcatggc cttcagggtg cacagccgca
      541 acggcaagag ttacacgttc ctgatctcct ctgactatga gcgtgcagag tggagggaga
      601 acatccggga gcagcagaag aagtgtttca gaagcttctc cctgacatcc gtggagctgc
      661 agatgctgac caactcgtgt gtgaaactcc agactgtcca cagcattccg ctgaccatca
      721 ataaggaaga tgatgagtct ccggggctct atgggtttct gaatgtcatc gtccactcag
      781 ccactggatt taagcagagt tcaaaagccc ttcagcggcc agtagcatct gactttgagc
      841 ctcagggtct gagtgaagcc gctcgttgga actccaagga aaaccttctc gctggaccca
      901 gtgaaaatga ccccaacctt ttcgttgcac tgtatgattt tgtggccagt ggagataaca
      961 ctctaagcat aactaaaggt gaaaagctcc gggtcttagg ctataatcac aatggggaat
     1021 ggtgtgaagc ccaaaccaaa aatggccaag gctgggtccc aagcaactac atcacgccag
     1081 tcaacagtct ggagaaacac tcctggtacc atgggcctgt gtcccgcaat gccgctgagt
     1141 atctgctgag cagcgggatc aatggcagct tcttggtgcg tgagagtgag agcagtcctg
     1201 gccagaggtc catctcgctg agatacgaag ggagggtgta ccattacagg atcaacactg
     1261 cttctgatgg caagctctac gtctcctccg agagccgctt caacaccctg gccgagttgg
     1321 ttcatcatca ttcaacggtg gccgacgggc tcatcaccac gctccattat ccagccccaa
     1381 agcgcaacaa gcccactgtc tatggtgtgt cccccaacta cgacaagtgg gagatggaac
     1441 gcacggacat caccatgaag cacaagctgg gcgggggcca gtacggggag gtgtacgagg
     1501 gcgtgtggaa gaaatacagc ctgacggtgg ccgtgaagac cttgaaggag gacaccatgg
     1561 aggtggaaga gttcttgaaa gaagctgcag tcatgaaaga gatcaaacac cctaacctgg
     1621 tgcagctcct tggggtctgc acccgggagc ccccgttcta tatcatcact gagttcatga
     1681 cctacgggaa cctcctggac tacctgaggg agtgcaaccg gcaggaggtg aacgccgtgg
     1741 tgctgctgta catggccact cagatctcgt cagccatgga gtacctggag aagaaaaact
     1801 tcatccacag agatcttgct gcccgaaact gcctggtagg ggagaaccac ttggtgaagg
     1861 tagctgattt tggcctgagc aggttgatga caggggacac ctacacagcc catgctggag
     1921 ccaagttccc catcaaatgg actgcacccg agagcctggc ctacaacaag ttctccatca
     1981 agtccgacgt ctgggcattt ggagtattgc tttgggaaat tgctacctat ggcatgtccc
     2041 cttacccggg aattgacctg tcccaggtgt atgagctgct agagaaggac taccgcatgg
     2101 agcgcccaga aggctgccca gagaaggtct atgaactcat gcgagcatgt tggcagtgga
     2161 atccctctga ccggccctcc tttgctgaaa tccaccaagc ctttgaaaca atgttccagg
     2221 aatccagtat ctcagacgaa gtggaaaagg agctggggaa acaaggcgtc cgtggggctg
     2281 tgagtacctt gctgcaggcc ccagagctgc ccaccaagac gaggacctcc aggagagctg
     2341 cagagcacag agacaccact gacgtgcctg agatgcctca ctccaagggc cagggagaga
     2401 gcgatcctct ggaccatgag cctgccgtgt ctccattgct ccctcgaaaa gagcgaggtc
     2461 ccccggaggg cggcctgaat gaagatgagc gccttctccc caaagacaaa aagaccaact
     2521 tgttcagcgc cttgatcaag aagaagaaga agacagcccc aacccctccc aaacgcagca
     2581 gctccttccg ggagatggac ggccagccgg agcgcagagg ggccggcgag gaagagggcc
     2641 gagacatcag caacggggca ctggctttca cccccttgga cacagctgac ccagccaagt
     2701 ccccaaagcc cagcaatggg gctggggtcc ccaatggagc cctccgggag tctgggggct
     2761 caggcttccg gtctccccac ctgtggaaga agtccagcac gctgaccagc agccgcctag
     2821 ccaccggcga ggaggagggc ggtggcagct ccagcaagcg cttcctgcgc tcttgctccg
     2881 cctcctgcgt tccccatggg gccaaggaca cggagtggag gtcagtcacg ctgcctcggg
     2941 acttgcagtc cacgggaaga cagtttgact cgtccacatt tggagggcac aaaagtgaga
     3001 agccggctct gcctcggaag agggcagggg agaacaggtc tgaccaggtg acccgaggca
     3061 cagtaacgcc tccccccagg ctggtgaaaa agaatgagga agctgctgat gaggtcttca
     3121 aagacatcat ggagtccagc ccgggctcca gcccgcccaa cctgactcca aaacccctcc
     3181 ggcggcaggt caccgtggcc cctgcctcgg gcctccccca caaggaagaa gctggaaagg
     3241 gcagtgcctt agggacccct gctgcagctg agccagtgac ccccaccagc aaagcaggct
     3301 caggtgcacc agggggcacc agcaagggcc ccgccgagga gtccagagtg aggaggcaca
     3361 agcactcctc tgagtcgcca gggagggaca aggggaaatt gtccaggctc aaacctgccc
     3421 cgccgccccc accagcagcc tctgcaggga aggctggagg aaagccctcg cagagcccga
     3481 gccaggaggc ggccggggag gcagtcctgg gcgcaaagac aaaagccacg agtctggttg
     3541 atgctgtgaa cagtgacgct gccaagccca gccagccggg agagggcctc aaaaagcccg
     3601 tgctcccggc cactccaaag ccacagtccg ccaagccgtc ggggaccccc atcagcccag
     3661 cccccgttcc ctccacgttg ccatcagcat cctcggccct ggcaggggac cagccgtctt
     3721 ccaccgcctt catccctctc atatcaaccc gagtgtctct tcggaaaacc cgccagcctc
     3781 cagagcggat cgccagcggc gccatcacca agggcgtggt cctggacagc accgaggcgc
     3841 tgtgcctcgc catctctagg aactccgagc agatggccag ccacagcgca gtgctggagg
     3901 ccggcaaaaa cctctacacg ttctgcgtga gctatgtgga ttccatccag caaatgagga
     3961 acaagtttgc cttccgagag gccatcaaca aactggagaa taatctccgg gagcttcaga
     4021 tctgcccggc gacagcaggc agtggtccag cggccactca ggacttcagc aagctcctca
     4081 gttcggtgaa ggaaatcagt gacatagtgc agaggtaggc ggccgctcga gtagatgact
     4141 agtctagagg gccctattct atagtgtcac ctaaatgcta gagctcgctg atcagcctcg
     4201 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4261 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4321 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4381 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4441 agaaccagtg gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat
     4501 gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt
     4561 ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg
     4621 aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc
     4681 tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt
     4741 ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa
     4801 gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta
     4861 tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa
     4921 caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa
     4981 ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt
     5041 cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt
     5101 ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat
     5161 cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat
     5221 gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc
     5281 aatctaaagt atatatgagt aacctgaggc tatggcaggg cctgccgccc cgacgttggc
     5341 tgcgagccct gggccttcac ccgaacttgg ggggtggggt ggggaaaagg aagaaacgcg
     5401 ggcgtattgg ccccaatggg gtctcggtgg ggtatcgaca gagtgccagc cctgggaccg
     5461 aaccccgcgt ttatgaacaa acgacccaac accgtgcgtt ttattctgtc tttttattgc
     5521 cgtcatagcg cgggttcctt ccggtattgt ctccttccgt gtttcagtta gcctccccct
     5581 agggtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc agccggcgtc
     5641 ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat cgaaatctcg
     5701 tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc cgctcagaag
     5761 aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc gataccgtaa
     5821 agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc acgggtagcc
     5881 aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat gaatccagaa
     5941 aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt cacgacgaga
     6001 tcctcgccgt cgggcatgct cgccttgagc ctggcgaaca gttcggctgg cgcgagcccc
     6061 tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg agtacgtgct
     6121 cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc aagcgtatgc
     6181 agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag gtgagatgac
     6241 aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc ttcagtgaca
     6301 acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag ccgcgctgcc
     6361 tcgtcttgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag aaccgggcgc
     6421 ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg ttgtgcccag
     6481 tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa tccatcttgt
     6541 tcaatcatgc gaaacgatcc tcatcctgtc tcttgatcga tctttgcaaa agcctaggcc
     6601 tccaaaaaag cctcctcact acttctggaa tagctcagag gccgaggcgg cctcggcctc
     6661 tgcataaata aaaaaaatta gtcagccatg gggcggagaa tgggcggaac tgggcggagt
     6721 taggggcggg atgggcggag ttaggggcgg gactatggtt gctgactaat tgagatgcat
     6781 gctttgcata cttctgcctg ctggggagcc tggggacttt ccacacctgg ttgctgacta
     6841 attgagatgc atgctttgca tacttctgcc tgctggggag cctggggact ttccacaccc
     6901 taactgacac acattccaca gctggttctt tccgcctcag gactcttcct ttttcaataa
     6961 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     7021 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     7081 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     7141 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     7201 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     7261 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     7321 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     7381 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     7441 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     7501 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     7561 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     7621 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     7681 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     7741 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     7801 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     7861 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     7921 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     7981 agtgccacct gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8041 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8101 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8161 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8221 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8281 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8341 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta
     8401 acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttacg cgcgcgttga
     8461 cattgattat tgactagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca
     8521 tatatggagt tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac
     8581 gacccccgcc cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact
     8641 ttccattgac gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa
     8701 gtgtatcata tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg
     8761 cattatgccc agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta
     8821 gtcatcgcta ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg
     8881 tttgactcac ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg
     8941 caccaaaatc aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg
     9001 ggcggtaggc gtgtacggtg ggaggtctat ataagcagag ctctctggct aactagagaa
     9061 cccactgctt actggcttat cgaaattaat acgactcact atagggagac ccaagcttgg
     9121 taccggatcc cccgggaatc aaaacatgga ttacaaagac gatgacgata aagaattctg
     9181 cagatatctg gtggacccgg tgggcttcgc ggaggcgtgg aaggcgcagt tcccggactc
     9241 agagcccccg cgcatggagc tgcgctcagt gggcgacatc gagcaggagc tggagcgctg
     9301 caaggcctcc attcggcgcc tggagcagga ggtgaaccag gagcgcttcc gcatgatcta
     9361 cctgcagacg ttgctggcca aggaaaagaa gagctatgac cggcagcgat ggggcttccg
     9421 gcgcgcggcg caggcccccg acggcgcctc cgagccccga gcgtccgcgt cgcgcccgca
     9481 gccagcgccc gccgacggag ccgacccgcc gcccgccgag gagcccgagg cccggcccga
     9541 cggcgagggt tctccgggta aggccaggcc cgggaccgcc cgcaggcccg gggcagccgc
     9601 gtcgggggaa cgggacgacc ggggaccccc cgccagcgtg gcggcgctca ggtccaactt
     9661 cgagcggata cgcaagggcc atggccagcc cggggcggac gccgagaagc ccttctacgt
     9721 gaacgtcgag tttcaccacg agcgcggcct ggtgaagttc gacagcagca gtccccccac
     9781 gccgcagtgc cataagcggc accggcactg cccggttgtc gtgtccgagg ccaccatcgt
     9841 gggcgtccgc aagaccgggc agatctggcc caacgatggc gagggcgcct tccatggaga
     9901 cgcagatggc tcgttcggaa caccacctgg atacggctgc gctgcagacc gggcagagga
     9961 gcagcgccgg caccaagatg ggctgcccta cattgatgac tcgccctcct catcgcccca
    10021 cctcagcagc aagggcaggg gcagccggga tgcgctggtc tcgggagccc tggagtccac
    10081 taaagcgagt gagctggact tggaaaaggg cttggagatg agaaaatggg tcctgtcggg
    10141 aatcctggct agcgaggaga cttacctgag ccacctggag gcactgctgc tgcccatgaa
    10201 gcctttgaaa gccgctgcca ccacctctca gccggtgctg acgagtcagc agatcgagac
    10261 catcttcttc aaagtgcctg agctctacga gatccacaag gagttctatg atgggctctt
    10321 cccccgcgtg cagcagtgga gccaccagca gcgggtgggc gacctcttcc agaagctggc
    10381 cagccagctg ggtgtgtacc gggccttcgt ggacaactac ggagttgcca tggaaatggc
    10441 tgagaagtgc tgtcaggcca atgctcagtt tgcagaaatc tccgagaacc tgagagccag
    10501 aagcaacaaa gatgccaagg atccaacgac caagaactct ctggaaactc tgctctacaa
    10561 gcctgtggac cgtgtgacga ggagcacgct ggtcctccat gact
//
