LOCUS       sequence-407737-       11165 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BCR-ABL oncogene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11165)
  AUTHORS   Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky 
            NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek 
            L, Krejci P
  TITLE     Elucidation of protein interactions necessary for the maintenance of
            the BCR-ABL signaling complex.
  JOURNAL   Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. doi: 
            10.1007/s00018-019-03397-7. Epub 2019 Dec 9.
  PUBMED    31820037
REFERENCE   2  (bases 1 to 11165)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-019-03397-7"; journalName: "Cell Mol Life Sci"; 
            date: "2020-10"; volume: "77"; issue: "19"; pages: "3885-3903"
COMMENT     Sequence Label: pcDNA3.1-BCR-ABL-p210-Y177F-FLAG
FEATURES             Location/Qualifiers
     source          1..11165
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(4439..4457)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(4440..4457)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(4477..4494)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4483..4707
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4839..5427)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4919..4938)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5756..5803
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5828..5847
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(6035..6829)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(6035..6829)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(6146..6165)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6756..6775
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6864..7221)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6878..7013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        7030..7221
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     7178..7198
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(7281..8072,8073..8141))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7904..7923
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8142..8246)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      8273..8728
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8360..8379)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8570..8591
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        8740..9119
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9120..9323
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9274..9294
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9368..9387
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9368..9386
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             9430..9453
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ccgcgtgcag cagtggagcc accagcagcg ggtgggcgac ctcttccaga agctggccag
       61 ccagctgggt gtgtaccggg ccttcgtgga caactacgga gttgccatgg aaatggctga
      121 gaagtgctgt caggccaatg ctcagtttgc agaaatctcc gagaacctga gagccagaag
      181 caacaaagat gccaaggatc caacgaccaa gaactctctg gaaactctgc tctacaagcc
      241 tgtggaccgt gtgacgagga gcacgctggt cctccatgac ttgctgaagc acactcctgc
      301 cagccaccct gaccacccct tgctgcagga cgccctccgc atctcacaga acttcctgtc
      361 cagcatcaat gaggagatca caccccgacg gcagtccatg acggtgaaga agggagagca
      421 ccggcagctg ctgaaggaca gcttcatggt ggagctggtg gagggggccc gcaagctgcg
      481 ccacgtcttc ctgttcaccg acctgcttct ctgcaccaag ctcaagaagc agagcggagg
      541 caaaacgcag cagtatgact gcaaatggta cattccgctc acggatctca gcttccagat
      601 ggtggatgaa ctggaggcag tgcccaacat ccccctggtg cccgatgagg agctggacgc
      661 tttgaagatc aagatctccc agatcaagag tgacatccag agagagaaga gggcgaacaa
      721 gggcagcaag gctacggaga ggctgaagaa gaagctgtcg gagcaggagt cactgctgct
      781 gcttatgtct cccagcatgg ccttcagggt gcacagccgc aacggcaaga gttacacgtt
      841 cctgatctcc tctgactatg agcgtgcaga gtggagggag aacatccggg agcagcagaa
      901 gaagtgtttc agaagcttct ccctgacatc cgtggagctg cagatgctga ccaactcgtg
      961 tgtgaaactc cagactgtcc acagcattcc gctgaccatc aataaggaag atgatgagtc
     1021 tccggggctc tatgggtttc tgaatgtcat cgtccactca gccactggat ttaagcagag
     1081 ttcaaaagcc cttcagcggc cagtagcatc tgactttgag cctcagggtc tgagtgaagc
     1141 cgctcgttgg aactccaagg aaaaccttct cgctggaccc agtgaaaatg accccaacct
     1201 tttcgttgca ctgtatgatt ttgtggccag tggagataac actctaagca taactaaagg
     1261 tgaaaagctc cgggtcttag gctataatca caatggggaa tggtgtgaag cccaaaccaa
     1321 aaatggccaa ggctgggtcc caagcaacta catcacgcca gtcaacagtc tggagaaaca
     1381 ctcctggtac catgggcctg tgtcccgcaa tgccgctgag tatctgctga gcagcgggat
     1441 caatggcagc ttcttggtgc gtgagagtga gagcagtcct ggccagaggt ccatctcgct
     1501 gagatacgaa gggagggtgt accattacag gatcaacact gcttctgatg gcaagctcta
     1561 cgtctcctcc gagagccgct tcaacaccct ggccgagttg gttcatcatc attcaacggt
     1621 ggccgacggg ctcatcacca cgctccatta tccagcccca aagcgcaaca agcccactgt
     1681 ctatggtgtg tcccccaact acgacaagtg ggagatggaa cgcacggaca tcaccatgaa
     1741 gcacaagctg ggcgggggcc agtacgggga ggtgtacgag ggcgtgtgga agaaatacag
     1801 cctgacggtg gccgtgaaga ccttgaagga ggacaccatg gaggtggaag agttcttgaa
     1861 agaagctgca gtcatgaaag agatcaaaca ccctaacctg gtgcagctcc ttggggtctg
     1921 cacccgggag cccccgttct atatcatcac tgagttcatg acctacggga acctcctgga
     1981 ctacctgagg gagtgcaacc ggcaggaggt gaacgccgtg gtgctgctgt acatggccac
     2041 tcagatctcg tcagccatgg agtacctgga gaagaaaaac ttcatccaca gagatcttgc
     2101 tgcccgaaac tgcctggtag gggagaacca cttggtgaag gtagctgatt ttggcctgag
     2161 caggttgatg acaggggaca cctacacagc ccatgctgga gccaagttcc ccatcaaatg
     2221 gactgcaccc gagagcctgg cctacaacaa gttctccatc aagtccgacg tctgggcatt
     2281 tggagtattg ctttgggaaa ttgctaccta tggcatgtcc ccttacccgg gaattgacct
     2341 gtcccaggtg tatgagctgc tagagaagga ctaccgcatg gagcgcccag aaggctgccc
     2401 agagaaggtc tatgaactca tgcgagcatg ttggcagtgg aatccctctg accggccctc
     2461 ctttgctgaa atccaccaag cctttgaaac aatgttccag gaatccagta tctcagacga
     2521 agtggaaaag gagctgggga aacaaggcgt ccgtggggct gtgagtacct tgctgcaggc
     2581 cccagagctg cccaccaaga cgaggacctc caggagagct gcagagcaca gagacaccac
     2641 tgacgtgcct gagatgcctc actccaaggg ccagggagag agcgatcctc tggaccatga
     2701 gcctgccgtg tctccattgc tccctcgaaa agagcgaggt cccccggagg gcggcctgaa
     2761 tgaagatgag cgccttctcc ccaaagacaa aaagaccaac ttgttcagcg ccttgatcaa
     2821 gaagaagaag aagacagccc caacccctcc caaacgcagc agctccttcc gggagatgga
     2881 cggccagccg gagcgcagag gggccggcga ggaagagggc cgagacatca gcaacggggc
     2941 actggctttc acccccttgg acacagctga cccagccaag tccccaaagc ccagcaatgg
     3001 ggctggggtc cccaatggag ccctccggga gtctgggggc tcaggcttcc ggtctcccca
     3061 cctgtggaag aagtccagca cgctgaccag cagccgccta gccaccggcg aggaggaggg
     3121 cggtggcagc tccagcaagc gcttcctgcg ctcttgctcc gcctcctgcg ttccccatgg
     3181 ggccaaggac acggagtgga ggtcagtcac gctgcctcgg gacttgcagt ccacgggaag
     3241 acagtttgac tcgtccacat ttggagggca caaaagtgag aagccggctc tgcctcggaa
     3301 gagggcaggg gagaacaggt ctgaccaggt gacccgaggc acagtaacgc ctccccccag
     3361 gctggtgaaa aagaatgagg aagctgctga tgaggtcttc aaagacatca tggagtccag
     3421 cccgggctcc agcccgccca acctgactcc aaaacccctc cggcggcagg tcaccgtggc
     3481 ccctgcctcg ggcctccccc acaaggaaga agctggaaag ggcagtgcct tagggacccc
     3541 tgctgcagct gagccagtga cccccaccag caaagcaggc tcaggtgcac cagggggcac
     3601 cagcaagggc cccgccgagg agtccagagt gaggaggcac aagcactcct ctgagtcgcc
     3661 agggagggac aaggggaaat tgtccaggct caaacctgcc ccgccgcccc caccagcagc
     3721 ctctgcaggg aaggctggag gaaagccctc gcagagcccg agccaggagg cggccgggga
     3781 ggcagtcctg ggcgcaaaga caaaagccac gagtctggtt gatgctgtga acagtgacgc
     3841 tgccaagccc agccagccgg gagagggcct caaaaagccc gtgctcccgg ccactccaaa
     3901 gccacagtcc gccaagccgt cggggacccc catcagccca gcccccgttc cctccacgtt
     3961 gccatcagca tcctcggccc tggcagggga ccagccgtct tccaccgcct tcatccctct
     4021 catatcaacc cgagtgtctc ttcggaaaac ccgccagcct ccagagcgga tcgccagcgg
     4081 cgccatcacc aagggcgtgg tcctggacag caccgaggcg ctgtgcctcg ccatctctag
     4141 gaactccgag cagatggcca gccacagcgc agtgctggag gccggcaaaa acctctacac
     4201 gttctgcgtg agctatgtgg attccatcca gcaaatgagg aacaagtttg ccttccgaga
     4261 ggccatcaac aaactggaga ataatctccg ggagcttcag atctgcccgg cgacagcagg
     4321 cagtggtcca gcggccactc aggacttcag caagctcctc agttcggtga aggaaatcag
     4381 tgacatagtg cagaggtagg cggccgctcg agtagatgac tagtctagag ggccctattc
     4441 tatagtgtca cctaaatgct agagctcgct gatcagcctc gactgtgcct tctagttgcc
     4501 agccatctgt tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca
     4561 ctgtcctttc ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta
     4621 ttctgggggg tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc
     4681 atgctgggga tgcggtgggc tctatggctt ctgaggcgga aagaaccagt ggcggtaata
     4741 cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     4801 aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     4861 gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     4921 agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     4981 cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     5041 cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     5101 ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     5161 gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     5221 tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga
     5281 acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     5341 tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     5401 attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     5461 gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     5521 ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     5581 taacctgagg ctatggcagg gcctgccgcc ccgacgttgg ctgcgagccc tgggccttca
     5641 cccgaacttg gggggtgggg tggggaaaag gaagaaacgc gggcgtattg gccccaatgg
     5701 ggtctcggtg gggtatcgac agagtgccag ccctgggacc gaaccccgcg tttatgaaca
     5761 aacgacccaa caccgtgcgt tttattctgt ctttttattg ccgtcatagc gcgggttcct
     5821 tccggtattg tctccttccg tgtttcagtt agcctccccc tagggtgggc gaagaactcc
     5881 agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac gattccgaag
     5941 cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc
     6001 gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca agaaggcgat
     6061 agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg aagcggtcag
     6121 cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg tcctgatagc
     6181 ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca ttttccacca
     6241 tgatattcgg caagcaggca tcgccatggg tcacgacgag atcctcgccg tcgggcatgc
     6301 tcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct tcgtccagat
     6361 catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg
     6421 cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc attgcatcag
     6481 ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc tgccccggca
     6541 cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc acagctgcgc
     6601 aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcttgc agttcattca
     6661 gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga
     6721 acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg aatagcctct
     6781 ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc
     6841 ctcatcctgt ctcttgatcg atctttgcaa aagcctaggc ctccaaaaaa gcctcctcac
     6901 tacttctgga atagctcaga ggccgaggcg gcctcggcct ctgcataaat aaaaaaaatt
     6961 agtcagccat ggggcggaga atgggcggaa ctgggcggag ttaggggcgg gatgggcgga
     7021 gttaggggcg ggactatggt tgctgactaa ttgagatgca tgctttgcat acttctgcct
     7081 gctggggagc ctggggactt tccacacctg gttgctgact aattgagatg catgctttgc
     7141 atacttctgc ctgctgggga gcctggggac tttccacacc ctaactgaca cacattccac
     7201 agctggttct ttccgcctca ggactcttcc tttttcaata aatcaatcta aagtatatat
     7261 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     7321 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     7381 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     7441 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     7501 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     7561 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     7621 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     7681 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     7741 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     7801 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     7861 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     7921 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     7981 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     8041 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     8101 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     8161 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     8221 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgcgccc
     8281 tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt
     8341 gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc
     8401 ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta
     8461 cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc
     8521 tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg
     8581 ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt
     8641 ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat
     8701 tttaacaaaa tattaacgct tacaatttac gcgcgcgttg acattgatta ttgactagtt
     8761 attaatagta atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta
     8821 cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt
     8881 caataatgac gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg
     8941 tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta
     9001 cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga
     9061 ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg
     9121 tgatgcggtt ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc
     9181 caagtctcca ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact
     9241 ttccaaaatg tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt
     9301 gggaggtcta tataagcaga gctctctggc taactagaga acccactgct tactggctta
     9361 tcgaaattaa tacgactcac tatagggaga cccaagcttg gtaccggatc ccccgggaat
     9421 caaaacatgg attacaaaga cgatgacgat aaagaattct gcagatatct ggtggacccg
     9481 gtgggcttcg cggaggcgtg gaaggcgcag ttcccggact cagagccccc gcgcatggag
     9541 ctgcgctcag tgggcgacat cgagcaggag ctggagcgct gcaaggcctc cattcggcgc
     9601 ctggagcagg aggtgaacca ggagcgcttc cgcatgatct acctgcagac gttgctggcc
     9661 aaggaaaaga agagctatga ccggcagcga tggggcttcc ggcgcgcggc gcaggccccc
     9721 gacggcgcct ccgagccccg agcgtccgcg tcgcgcccgc agccagcgcc cgccgacgga
     9781 gccgacccgc cgcccgccga ggagcccgag gcccggcccg acggcgaggg ttctccgggt
     9841 aaggccaggc ccgggaccgc ccgcaggccc ggggcagccg cgtcggggga acgggacgac
     9901 cggggacccc ccgccagcgt ggcggcgctc aggtccaact tcgagcggat acgcaagggc
     9961 catggccagc ccggggcgga cgccgagaag cccttcttcg tgaacgtcga gtttcaccac
    10021 gagcgcggcc tggtgaaggt caacgacaaa gaggtgtcgg accgcatcag ctccctgggc
    10081 agccaggcca tgcagatgga gcgcaaaaag tcccagcacg gcgcgggctc gagcgtgggg
    10141 gatgcatcca ggccccctta ccggggacgc tcctcggaga gcagctgcgg cgtcgacggc
    10201 gactacgagg acgccgagtt gaacccccgc ttcctgaagg acaacctgat cgacgccaat
    10261 ggcggtagca ggcccccttg gccgcccctg gagtaccagc cctaccagag catctacgtc
    10321 gggggcatga tggaagggga gggcaagggc ccgctcctgc gcagccagag cacctctgag
    10381 caggagaagc gccttacctg gccccgcagg tcctactccc cccggagttt tgaggattgc
    10441 ggaggcggct ataccccgga ctgcagctcc aatgagaacc tcacctccag cgaggaggac
    10501 ttctcctctg gccagtccag ccgcgtgtcc ccaagcccca ccacctaccg catgttccgg
    10561 gacaaaagcc gctctccctc gcagaactcg caacagtcct tcgacagcag cagtcccccc
    10621 acgccgcagt gccataagcg gcaccggcac tgcccggttg tcgtgtccga ggccaccatc
    10681 gtgggcgtcc gcaagaccgg gcagatctgg cccaacgatg gcgagggcgc cttccatgga
    10741 gacgcagatg gctcgttcgg aacaccacct ggatacggct gcgctgcaga ccgggcagag
    10801 gagcagcgcc ggcaccaaga tgggctgccc tacattgatg actcgccctc ctcatcgccc
    10861 cacctcagca gcaagggcag gggcagccgg gatgcgctgg tctcgggagc cctggagtcc
    10921 actaaagcga gtgagctgga cttggaaaag ggcttggaga tgagaaaatg ggtcctgtcg
    10981 ggaatcctgg ctagcgagga gacttacctg agccacctgg aggcactgct gctgcccatg
    11041 aagcctttga aagccgctgc caccacctct cagccggtgc tgacgagtca gcagatcgag
    11101 accatcttct tcaaagtgcc tgagctctac gagatccaca aggagttcta tgatgggctc
    11161 ttccc
//
