LOCUS       sequence-407784-        9662 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BCR-ABL oncogene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9662)
  AUTHORS   Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky 
            NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek 
            L, Krejci P
  TITLE     Elucidation of protein interactions necessary for the maintenance of
            the BCR-ABL signaling complex.
  JOURNAL   Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. doi: 
            10.1007/s00018-019-03397-7. Epub 2019 Dec 9.
  PUBMED    31820037
REFERENCE   2  (bases 1 to 9662)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-019-03397-7"; journalName: "Cell Mol Life Sci"; 
            date: "2020-10"; volume: "77"; issue: "19"; pages: "3885-3903"
COMMENT     Sequence Label: pcDNA3.1-BCR-ABL-p190-FLAG
FEATURES             Location/Qualifiers
     source          1..9662
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(3687..3705)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3688..3705)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3725..3742)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3731..3955
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4087..4675)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4167..4186)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5004..5051
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5076..5095
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5283..6077)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5283..6077)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5394..5413)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6004..6023
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6112..6469)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6126..6261
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6278..6469
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6426..6446
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(6529..7320,7321..7389))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7152..7171
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7390..7494)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7521..7976
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7608..7627)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7818..7839
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        7988..8367
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8368..8571
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8522..8542
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8616..8635
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8616..8634
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8678..8701
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 actccccccg gagttttgag gattgcggag gcggctatac cccggactgc agctccaatg
       61 agaacctcac ctccagcgag gaggacttct cctctggcca gtccagccgc gtgtccccaa
      121 gccccaccac ctaccgcatg ttccgggaca aaagccgctc tccctcgcag aactcgcaac
      181 agtccttcga cagcagcagt ccccccacgc cgcagtgcca taagcggcac cggcactgcc
      241 cggttgtcgt gtccgaggcc accatcgtgg gcgtccgcaa gaccgggcag atctggccca
      301 acgatggcga gggcgccttc catggagacg cagaagccct tcagcggcca gtagcatctg
      361 actttgagcc tcagggtctg agtgaagccg ctcgttggaa ctccaaggaa aaccttctcg
      421 ctggacccag tgaaaatgac cccaaccttt tcgttgcact gtatgatttt gtggccagtg
      481 gagataacac tctaagcata actaaaggtg aaaagctccg ggtcttaggc tataatcaca
      541 atggggaatg gtgtgaagcc caaaccaaaa atggccaagg ctgggtccca agcaactaca
      601 tcatgccagt caacagtctg gagaaacact cctggtacca tgggcctgtg tcccgcaatg
      661 ccgctgagta tctgctgagc agcgggatca atggcagctt cttggtgcgt gagagtgaga
      721 gcagtcctgg ccagaggtcc atctcgctga gatacgaagg gagggtgtac cattacagga
      781 tcaacactgc ttctgatggc aagctctacg tctcctccga gagccgcttc aacaccctgg
      841 ccgagttggt tcatcatcat tcaacggtgg ccgacgggct catcaccacg ctccattatc
      901 cagccccaaa gcgcaacaag cccactgtct atggtgtgtc ccccaactac gacaagtggg
      961 agatggaacg cacggacatc accatgaagc acaagctggg cgggggccag tacggggagg
     1021 tgtacgaggg cgtgtggaag aaatacagcc tgacggtggc cgtgaagacc ttgaaggagg
     1081 acaccatgga ggtggaagag ttcttgaaag aagctgcagt catgaaagag atcaaacacc
     1141 ctaacctggt gcagctcctt ggggtctgca cccgggagcc cccgttctat atcatcactg
     1201 agttcatgac ctacgggaac ctcctggact acctgaggga gtgcaaccgg caggaggtga
     1261 acgccgtggt gctgctgtac atggccactc agatctcgtc agccatggag tacctggaga
     1321 agaaaaactt catccacaga gatcttgctg cccgaaactg cctggtaggg gagaaccact
     1381 tggtgaaggt agctgatttt ggcctgagca ggttgatgac aggggacacc tacacagccc
     1441 atgctggagc caagttcccc atcaaatgga ctgcacccga gagcctggcc tacaacaagt
     1501 tctccatcaa gtccgacgtc tgggcatttg gagtattgct ttgggaaatt gctacctatg
     1561 gcatgtcccc ttacccggga attgacctgt cccaggtgta tgagctgcta gagaaggact
     1621 accgcatgga gcgcccagaa ggctgcccag agaaggtcta tgaactcatg cgagcatgtt
     1681 ggcagtggaa tccctctgac cggccctcct ttgctgaaat ccaccaagcc tttgaaacaa
     1741 tgttccagga atccagtatc tcagacgaag tggaaaagga gctggggaaa caaggcgtcc
     1801 gtggggctgt gagtaccttg ctgcaggccc cagagctgcc caccaagacg aggacctcca
     1861 ggagagctgc agagcacaga gacaccactg acgtgcctga gatgcctcac tccaagggcc
     1921 agggagagag cgatcctctg gaccatgagc ctgccgtgtc tccattgctc cctcgaaaag
     1981 agcgaggtcc cccggagggc ggcctgaatg aagatgagcg ccttctcccc aaagacaaaa
     2041 agaccaactt gttcagcgcc ttgatcaaga agaagaagaa gacagcccca acccctccca
     2101 aacgcagcag ctccttccgg gagatggacg gccagccgga gcgcagaggg gccggcgagg
     2161 aagagggccg agacatcagc aacggggcac tggctttcac ccccttggac acagctgacc
     2221 cagccaagtc cccaaagccc agcaatgggg ctggggtccc caatggagcc ctccgggagt
     2281 ccgggggctc aggcttccgg tctccccacc tgtggaagaa gtccagcacg ctgaccagca
     2341 gccgcctagc caccggcgag gaggagggcg gtggcagctc cagcaagcgc ttcctgcgct
     2401 cttgctccgc ctcctgcgtt ccccatgggg ccaaggacac ggagtggagg tcagtcacgc
     2461 tgcctcggga cttgcagtcc acgggaagac agtttgactc gtccacattt ggagggcaca
     2521 aaagtgagaa gccggctctg cctcggaaga gggcagggga gaacaggtct gaccaggtga
     2581 cccgaggcac agtaacgcct ccccccaggc tggtgaaaaa gaatgaggaa gctgctgatg
     2641 aggtcttcaa agacatcatg gagtccagcc cgggctccag cccgcccaac ctgactccaa
     2701 aacccctccg gcggcaggtc accgtggccc ctgcctcggg cctcccccac aaggaagaag
     2761 ctggaaaggg cagtgcctta gggacccctg ctgcagctga gccagtgacc cccaccagca
     2821 aagcaggctc aggtgcacca gggggcacca gcaagggccc cgccgaggag tccagagtga
     2881 ggaggcacaa gcactcctct gagtcgccag ggagggacaa ggggaaattg tccaggctca
     2941 aacctgcccc gccgccccca ccagcagcct ctgcagggaa ggctggagga aagccctcgc
     3001 agagcccgag ccaggaggcg gccggggagg cagtcctggg cgcaaagaca aaagccacga
     3061 gtctggttga tgctgtgaac agtgacgctg ccaagcccag ccagccggga gagggcctca
     3121 aaaagcccgt gctcccggcc actccaaagc cacagtccgc caagccgtcg gggaccccca
     3181 tcagcccagc ccccgttccc tccacgttgc catcagcatc ctcggccctg gcaggggacc
     3241 agccgtcttc caccgccttc atccctctca tatcaacccg agtgtctctt cggaaaaccc
     3301 gccagcctcc agagcggatc gccagcggcg ccatcaccaa gggcgtggtc ctggacagca
     3361 ccgaggcgct gtgcctcgcc atctctagga actccgagca gatggccagc cacagcgcag
     3421 tgctggaggc cggcaaaaac ctctacacgt tctgcgtgag ctatgtggat tccatccagc
     3481 aaatgaggaa caagtttgcc ttccgagagg ccatcaacaa actggagaat aatctccggg
     3541 agcttcagat ctgcccggcg acagcaggca gtggtccagc ggccactcag gacttcagca
     3601 agctcctcag ttcggtgaag gaaatcagtg acatagtgca gaggtaggcg gccgctcgag
     3661 tagatgacta gtctagaggg ccctattcta tagtgtcacc taaatgctag agctcgctga
     3721 tcagcctcga ctgtgccttc tagttgccag ccatctgttg tttgcccctc ccccgtgcct
     3781 tccttgaccc tggaaggtgc cactcccact gtcctttcct aataaaatga ggaaattgca
     3841 tcgcattgtc tgagtaggtg tcattctatt ctggggggtg gggtggggca ggacagcaag
     3901 ggggaggatt gggaagacaa tagcaggcat gctggggatg cggtgggctc tatggcttct
     3961 gaggcggaaa gaaccagtgg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     4021 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     4081 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     4141 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     4201 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     4261 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     4321 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     4381 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     4441 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     4501 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     4561 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     4621 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     4681 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     4741 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     4801 ttttaaatca atctaaagta tatatgagta acctgaggct atggcagggc ctgccgcccc
     4861 gacgttggct gcgagccctg ggccttcacc cgaacttggg gggtggggtg gggaaaagga
     4921 agaaacgcgg gcgtattggc cccaatgggg tctcggtggg gtatcgacag agtgccagcc
     4981 ctgggaccga accccgcgtt tatgaacaaa cgacccaaca ccgtgcgttt tattctgtct
     5041 ttttattgcc gtcatagcgc gggttccttc cggtattgtc tccttccgtg tttcagttag
     5101 cctcccccta gggtgggcga agaactccag catgagatcc ccgcgctgga ggatcatcca
     5161 gccggcgtcc cggaaaacga ttccgaagcc caacctttca tagaaggcgg cggtggaatc
     5221 gaaatctcgt gatggcaggt tgggcgtcgc ttggtcggtc atttcgaacc ccagagtccc
     5281 gctcagaaga actcgtcaag aaggcgatag aaggcgatgc gctgcgaatc gggagcggcg
     5341 ataccgtaaa gcacgaggaa gcggtcagcc cattcgccgc caagctcttc agcaatatca
     5401 cgggtagcca acgctatgtc ctgatagcgg tccgccacac ccagccggcc acagtcgatg
     5461 aatccagaaa agcggccatt ttccaccatg atattcggca agcaggcatc gccatgggtc
     5521 acgacgagat cctcgccgtc gggcatgctc gccttgagcc tggcgaacag ttcggctggc
     5581 gcgagcccct gatgctcttc gtccagatca tcctgatcga caagaccggc ttccatccga
     5641 gtacgtgctc gctcgatgcg atgtttcgct tggtggtcga atgggcaggt agccggatca
     5701 agcgtatgca gccgccgcat tgcatcagcc atgatggata ctttctcggc aggagcaagg
     5761 tgagatgaca ggagatcctg ccccggcact tcgcccaata gcagccagtc ccttcccgct
     5821 tcagtgacaa cgtcgagcac agctgcgcaa ggaacgcccg tcgtggccag ccacgatagc
     5881 cgcgctgcct cgtcttgcag ttcattcagg gcaccggaca ggtcggtctt gacaaaaaga
     5941 accgggcgcc cctgcgctga cagccggaac acggcggcat cagagcagcc gattgtctgt
     6001 tgtgcccagt catagccgaa tagcctctcc acccaagcgg ccggagaacc tgcgtgcaat
     6061 ccatcttgtt caatcatgcg aaacgatcct catcctgtct cttgatcgat ctttgcaaaa
     6121 gcctaggcct ccaaaaaagc ctcctcacta cttctggaat agctcagagg ccgaggcggc
     6181 ctcggcctct gcataaataa aaaaaattag tcagccatgg ggcggagaat gggcggaact
     6241 gggcggagtt aggggcggga tgggcggagt taggggcggg actatggttg ctgactaatt
     6301 gagatgcatg ctttgcatac ttctgcctgc tggggagcct ggggactttc cacacctggt
     6361 tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     6421 tccacaccct aactgacaca cattccacag ctggttcttt ccgcctcagg actcttcctt
     6481 tttcaataaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt
     6541 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact
     6601 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat
     6661 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg
     6721 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg
     6781 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat
     6841 tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc
     6901 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt
     6961 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc
     7021 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga
     7081 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc
     7141 gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa
     7201 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta
     7261 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg
     7321 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg
     7381 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat
     7441 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt
     7501 tccccgaaaa gtgccacctg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt
     7561 ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt
     7621 cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct
     7681 ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg
     7741 tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga
     7801 gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc
     7861 ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga
     7921 gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta caatttacgc
     7981 gcgcgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
     8041 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
     8101 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
     8161 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
     8221 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg
     8281 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc
     8341 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt
     8401 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
     8461 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg
     8521 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tctctggcta
     8581 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
     8641 caagcttggt accggatccc ccgggaatca aaacatggat tacaaagacg atgacgataa
     8701 agaattctgc agatatctgg tggacccggt gggcttcgcg gaggcgtgga aggcgcagtt
     8761 cccggactca gagcccccgc gcatggagct gcgctcagtg ggcgacatcg agcaggagct
     8821 ggagcgctgc aaggcctcca ttcggcgcct ggagcaggag gtgaaccagg agcgcttccg
     8881 catgatctac ctgcagacgt tgctggccaa ggaaaagaag agctatgacc ggcagcgatg
     8941 gggcttccgg cgcgcggcgc aggcccccga cggcgcctcc gagccccgag cgtccgcgtc
     9001 gcgcccgcag ccagcgcccg ccgacggagc cgacccgccg cccgccgagg agcccgaggc
     9061 ccggcccgac ggcgagggtt ctccgggtaa ggccaggccc gggaccgccc gcaggcccgg
     9121 ggcagccgcg tcgggggaac gggacgaccg gggacccccc gccagcgtgg cggcgctcag
     9181 gtccaacttc gagcggatac gcaagggcca tggccagccc ggggcggacg ccgagaagcc
     9241 cttctacgtg aacgtcgagt ttcaccacga gcgcggcctg gtgaaggtca acgacaaaga
     9301 ggtgtcggac cgcatcagct ccctgggcag ccaggccatg cagatggagc gcaaaaagtc
     9361 ccagcacggc gcgggctcga gcgtggggga tgcatccagg cccccttacc ggggacgctc
     9421 ctcggagagc agctgcggcg tcgacggcga ctacgaggac gccgagttga acccccgctt
     9481 cctgaaggac aacctgatcg acgccaatgg cggtagcagg cccccttggc cgcccctgga
     9541 gtaccagccc taccagagca tctacgtcgg gggcatgatg gaaggggagg gcaagggccc
     9601 gctcctgcgc agccagagca cctctgagca ggagaagcgc cttacctggc cccgcaggtc
     9661 ct
//
