LOCUS       sequence-407787-       10487 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BCR-ABL oncogene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10487)
  AUTHORS   Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky 
            NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek 
            L, Krejci P
  TITLE     Elucidation of protein interactions necessary for the maintenance of
            the BCR-ABL signaling complex.
  JOURNAL   Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. doi: 
            10.1007/s00018-019-03397-7. Epub 2019 Dec 9.
  PUBMED    31820037
REFERENCE   2  (bases 1 to 10487)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-019-03397-7"; journalName: "Cell Mol Life Sci"; 
            date: "2020-10"; volume: "77"; issue: "19"; pages: "3885-3903"
COMMENT     Sequence Label: pcDNA3.1-BCR-ABL-p210-Delta-DH-FLAG
FEATURES             Location/Qualifiers
     source          1..10487
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(3030..3053)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3096..3115)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3097..3115)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3160..3363)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3189..3209)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3364..3743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3755..4210)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3892..3913)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4104..4123
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4237..4341
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4342..4410,4411..5202)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4560..4579)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        5262..5619
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5262..5453
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5285..5305)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5470..5605
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5654..6448
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5654..6448
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5708..5727)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6318..6337
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6636..6655)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6680..6727
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7056..7644
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7545..7564
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7776..8000
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7989..8006
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        8026..8044
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8026..8043
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 cactccctca ggtagtccag gaggttcccg taggtcatga actcagtgat gatatagaac
       61 gggggctccc gggtgcagac cccaaggagc tgcaccaggt tagggtgttt gatctctttc
      121 atgactgcag cttctttcaa gaactcttcc acctccatgg tgtcctcctt caaggtcttc
      181 acggccaccg tcaggctgta tttcttccac acgccctcgt acacctcccc gtactggccc
      241 ccgcccagct tgtgcttcat ggtgatgtcc gtgcgttcca tctcccactt gtcgtagttg
      301 ggggacacac catagacagt gggcttgttg cgctttgggg ctggataatg gagcgtggtg
      361 atgagcccgt cggccaccgt tgaatgatga tgaaccaact cggccagggt gttgaagcgg
      421 ctctcggagg agacgtagag cttgccatca gaagcagtgt tgatcctgta atggtacacc
      481 ctcccttcgt atctcagcga gatggacctc tggccaggac tgctctcact ctcacgcacc
      541 aagaagctgc cattgatccc gctgctcagc agatactcag cggcattgcg ggacacaggc
      601 ccatggtacc aggagtgttt ctccagactg ttgactggcg tgatgtagtt gcttgggacc
      661 cagccttggc catttttggt ttgggcttca caccattccc cattgtgatt atagcctaag
      721 acccggagct tttcaccttt agttatgctt agagtgttat ctccactggc cacaaaatca
      781 tacagtgcaa cgaaaaggtt ggggtcattt tcactgggtc cagcgagaag gttttccttg
      841 gagttccaac gagcggcttc actcagaccc tgaggctcaa agtcagatgc tactggccgc
      901 tgaagggctt ttgaactctg cttaaatcca gtggctgagt ggacgatgac attcagaaac
      961 ccatagagcc ccggagactc atcatcttcc ttattgatgg tcagcggaat gctgtggaca
     1021 gtctggagtt tcacacacga gttggtcagc atctgcagct ccacggatgt cagggagaag
     1081 cttctgaaac acttcttctg ctgctcccgg atgttctccc tccactctgc acgctcatag
     1141 tcagaggaga tcaggaacgt gtaactcttg ccgttgcggc tgtgcaccct gaaggccatg
     1201 ctgggagaca taagcagcag cagtgactcc tgctccgaca gcttcttctt cagcctctcc
     1261 gtagccttgc tgcccttgtt cgccctcttc tctctctgga tgtcactctt gatctgggag
     1321 atcttgatct tcaaagcgtc cagctcctca tcgggcacca gggggatgtt gggcactgcc
     1381 tccagttcat ccaccatctg gaagctgaga tccgtgagcg gaatgtacca tttgcagtca
     1441 tactgctgcg ttttgcctcc gctctgcttc ttgagcttgg tgcagagaag caggtcggtg
     1501 aacaggaaga cgtggcgcag cttgcgggcc ccctccacca gctccaccat gaagctgtcc
     1561 ttcagcagct gccggtgctc tcccttcttc accgtcatgg actgccgtcg gctgctgagg
     1621 tggggcgatg aggagggcga gtcatcaatg tagggcagcc catcttggtg ccggcgctgc
     1681 tcctctgccc ggtctgcagc gcagccgtat ccaggtggtg ttccgaacga gccatctgcg
     1741 tctccatgga aggcgccctc gccatcgttg ggccagatct gcccggtctt gcggacgccc
     1801 acgatggtgg cctcggacac gacaaccggg cagtgccggt gccgcttatg gcactgcggc
     1861 gtggggggac tgctgctgtc gaaggactgt tgcgagttct gcgagggaga gcggcttttg
     1921 tcccggaaca tgcggtaggt ggtggggctt ggggacacgc ggctggactg gccagaggag
     1981 aagtcctcct cgctggaggt gaggttctca ttggagctgc agtccggggt atagccgcct
     2041 ccgcaatcct caaaactccg gggggagtag gacctgcggg gccaggtaag gcgcttctcc
     2101 tgctcagagg tgctctggct gcgcaggagc gggcccttgc cctccccttc catcatgccc
     2161 ccgacgtaga tgctctggta gggctggtac tccaggggcg gccaaggggg cctgctaccg
     2221 ccattggcgt cgatcaggtt gtccttcagg aagcgggggt tcaactcggc gtcctcgtag
     2281 tcgccgtcga cgccgcagct gctctccgag gagcgtcccc ggtaaggggg cctggatgca
     2341 tcccccacgc tcgagcccgc gccgtgctgg gactttttgc gctccatctg catggcctgg
     2401 ctgcccaggg agctgatgcg gtccgacacc tctttgtcgt tgaccttcac caggccgcgc
     2461 tcgtggtgaa actcgacgtt cacgtagaag ggcttctcgg cgtccgcccc gggctggcca
     2521 tggcccttgc gtatccgctc gaagttggac ctgagcgccg ccacgctggc ggggggtccc
     2581 cggtcgtccc gttcccccga cgcggctgcc ccgggcctgc gggcggtccc gggcctggcc
     2641 ttacccggag aaccctcgcc gtcgggccgg gcctcgggct cctcggcggg cggcgggtcg
     2701 gctccgtcgg cgggcgctgg ctgcgggcgc gacgcggacg ctcggggctc ggaggcgccg
     2761 tcgggggcct gcgccgcgcg ccggaagccc catcgctgcc ggtcatagct cttcttttcc
     2821 ttggccagca acgtctgcag gtagatcatg cggaagcgct cctggttcac ctcctgctcc
     2881 aggcgccgaa tggaggcctt gcagcgctcc agctcctgct cgatgtcgcc cactgagcgc
     2941 agctccatgc gcgggggctc tgagtccggg aactgcgcct tccacgcctc cgcgaagccc
     3001 accgggtcca ccagatatct gcagaattct ttatcgtcat cgtctttgta atccatgttt
     3061 tgattcccgg gggatccggt accaagcttg ggtctcccta tagtgagtcg tattaatttc
     3121 gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta tatagacctc
     3181 ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac gacattttgg
     3241 aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg ggtggagact
     3301 tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc aaaaccgcat
     3361 caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa agtcccataa
     3421 ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt caataggggg
     3481 cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc gtaaatactc
     3541 cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat acgtcattat
     3601 tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta ccgtaagtta
     3661 tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg attactatta
     3721 ataactagtc aataatcaat gtcaacgcgc gcgtaaattg taagcgttaa tattttgtta
     3781 aaattcgcgt taaatttttg ttaaatcagc tcatttttta accaataggc cgaaatcggc
     3841 aaaatccctt ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg
     3901 aacaagagtc cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat
     3961 cagggcgatg gcccactacg tgaaccatca ccctaatcaa gttttttggg gtcgaggtgc
     4021 cgtaaagcac taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag
     4081 ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg
     4141 gcaagtgtag cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta
     4201 cagggcgcgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
     4261 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
     4321 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
     4381 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
     4441 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
     4501 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
     4561 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
     4621 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
     4681 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
     4741 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
     4801 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
     4861 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
     4921 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
     4981 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
     5041 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
     5101 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     5161 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     5221 tcatatatac tttagattga tttattgaaa aaggaagagt cctgaggcgg aaagaaccag
     5281 ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag gctccccagc aggcagaagt
     5341 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca
     5401 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
     5461 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     5521 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     5581 tagtgaggag gcttttttgg aggcctaggc ttttgcaaag atcgatcaag agacaggatg
     5641 aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt
     5701 ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt
     5761 gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc
     5821 cctgaatgaa ctgcaagacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc
     5881 ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga
     5941 agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat
     6001 ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca
     6061 agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga
     6121 tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc
     6181 gagcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat
     6241 catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga
     6301 ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg
     6361 ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt
     6421 ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat gaccgaccaa
     6481 gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg
     6541 ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg
     6601 ctggagttct tcgcccaccc tagggggagg ctaactgaaa cacggaagga gacaataccg
     6661 gaaggaaccc gcgctatgac ggcaataaaa agacagaata aaacgcacgg tgttgggtcg
     6721 tttgttcata aacgcggggt tcggtcccag ggctggcact ctgtcgatac cccaccgaga
     6781 ccccattggg gccaatacgc ccgcgtttct tccttttccc caccccaccc cccaagttcg
     6841 ggtgaaggcc cagggctcgc agccaacgtc ggggcggcag gccctgccat agcctcaggt
     6901 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6961 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     7021 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     7081 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     7141 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     7201 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     7261 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     7321 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     7381 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     7441 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     7501 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     7561 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     7621 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     7681 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     7741 cgtattaccg ccactggttc tttccgcctc agaagccata gagcccaccg catccccagc
     7801 atgcctgcta ttgtcttccc aatcctcccc cttgctgtcc tgccccaccc caccccccag
     7861 aatagaatga cacctactca gacaatgcga tgcaatttcc tcattttatt aggaaaggac
     7921 agtgggagtg gcaccttcca gggtcaagga aggcacgggg gaggggcaaa caacagatgg
     7981 ctggcaacta gaaggcacag tcgaggctga tcagcgagct ctagcattta ggtgacacta
     8041 tagaataggg ccctctagac tagtcatcta ctcgagcggc cgcctacctc tgcactatgt
     8101 cactgatttc cttcaccgaa ctgaggagct tgctgaagtc ctgagtggcc gctggaccac
     8161 tgcctgctgt cgccgggcag atctgaagct cccggagatt attctccagt ttgttgatgg
     8221 cctctcggaa ggcaaacttg ttcctcattt gctggatgga atccacatag ctcacgcaga
     8281 acgtgtagag gtttttgccg gcctccagca ctgcgctgtg gctggccatc tgctcggagt
     8341 tcctagagat ggcgaggcac agcgcctcgg tgctgtccag gaccacgccc ttggtgatgg
     8401 cgccgctggc gatccgctct ggaggctggc gggttttccg aagagacact cgggttgata
     8461 tgagagggat gaaggcggtg gaagacggct ggtcccctgc cagggccgag gatgctgatg
     8521 gcaacgtgga gggaacgggg gctgggctga tgggggtccc cgacggcttg gcggactgtg
     8581 gctttggagt ggccgggagc acgggctttt tgaggccctc tcccggctgg ctgggcttgg
     8641 cagcgtcact gttcacagca tcaaccagac tcgtggcttt tgtctttgcg cccaggactg
     8701 cctccccggc cgcctcctgg ctcgggctct gcgagggctt tcctccagcc ttccctgcag
     8761 aggctgctgg tgggggcggc ggggcaggtt tgagcctgga caatttcccc ttgtccctcc
     8821 ctggcgactc agaggagtgc ttgtgcctcc tcactctgga ctcctcggcg gggcccttgc
     8881 tggtgccccc tggtgcacct gagcctgctt tgctggtggg ggtcactggc tcagctgcag
     8941 caggggtccc taaggcactg ccctttccag cttcttcctt gtgggggagg cccgaggcag
     9001 gggccacggt gacctgccgc cggaggggtt ttggagtcag gttgggcggg ctggagcccg
     9061 ggctggactc catgatgtct ttgaagacct catcagcagc ttcctcattc tttttcacca
     9121 gcctgggggg aggcgttact gtgcctcggg tcacctggtc agacctgttc tcccctgccc
     9181 tcttccgagg cagagccggc ttctcacttt tgtgccctcc aaatgtggac gagtcaaact
     9241 gtcttcccgt ggactgcaag tcccgaggca gcgtgactga cctccactcc gtgtccttgg
     9301 ccccatgggg aacgcaggag gcggagcaag agcgcaggaa gcgcttgctg gagctgccac
     9361 cgccctcctc ctcgccggtg gctaggcggc tgctggtcag cgtgctggac ttcttccaca
     9421 ggtggggaga ccggaagcct gagcccccag actcccggag ggctccattg gggaccccag
     9481 ccccattgct gggctttggg gacttggctg ggtcagctgt gtccaagggg gtgaaagcca
     9541 gtgccccgtt gctgatgtct cggccctctt cctcgccggc ccctctgcgc tccggctggc
     9601 cgtccatctc ccggaaggag ctgctgcgtt tgggaggggt tggggctgtc ttcttcttct
     9661 tcttgatcaa ggcgctgaac aagttggtct ttttgtcttt ggggagaagg cgctcatctt
     9721 cattcaggcc gccctccggg ggacctcgct cttttcgagg gagcaatgga gacacggcag
     9781 gctcatggtc cagaggatcg ctctctccct ggcccttgga gtgaggcatc tcaggcacgt
     9841 cagtggtgtc tctgtgctct gcagctctcc tggaggtcct cgtcttggtg ggcagctctg
     9901 gggcctgcag caaggtactc acagccccac ggacgccttg tttccccagc tccttttcca
     9961 cttcgtctga gatactggat tcctggaaca ttgtttcaaa ggcttggtgg atttcagcaa
    10021 aggagggccg gtcagaggga ttccactgcc aacatgctcg catgagttca tagaccttct
    10081 ctgggcagcc ttctgggcgc tccatgcggt agtccttctc tagcagctca tacacctggg
    10141 acaggtcaat tcccgggtaa ggggacatgc cataggtagc aatttcccaa agcaatactc
    10201 caaatgccca gacgtcggac ttgatggaga acttgttgta ggccaggctc tcgggtgcag
    10261 tccatttgat ggggaacttg gctccagcat gggctgtgta ggtgtcccct gtcatcaacc
    10321 tgctcaggcc aaaatcagct accttcacca agtggttctc ccctaccagg cagtttcggg
    10381 cagcaagatc tctgtggatg aagtttttct tctccaggta ctccatggct gacgagatct
    10441 gagtggccat gtacagcagc accacggcgt tcacctcctg ccggttg
//
