LOCUS       sequence-407788-       10844 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BCR-ABL oncogene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10844)
  AUTHORS   Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky 
            NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek 
            L, Krejci P
  TITLE     Elucidation of protein interactions necessary for the maintenance of
            the BCR-ABL signaling complex.
  JOURNAL   Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. doi: 
            10.1007/s00018-019-03397-7. Epub 2019 Dec 9.
  PUBMED    31820037
REFERENCE   2  (bases 1 to 10844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-019-03397-7"; journalName: "Cell Mol Life Sci"; 
            date: "2020-10"; volume: "77"; issue: "19"; pages: "3885-3903"
COMMENT     Sequence Label: pcDNA3.1-BCR-ABL-p210-Delta-FA-FLAG
FEATURES             Location/Qualifiers
     source          1..10844
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(3208..3231)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3274..3293)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3275..3293)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3338..3541)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3367..3387)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3542..3921
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3933..4388)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4070..4091)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4282..4301
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4415..4519
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4520..4588,4589..5380)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4738..4757)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        5440..5797
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5440..5631
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5463..5483)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5648..5783
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5832..6626
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5832..6626
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5886..5905)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6496..6515
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6814..6833)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6858..6905
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7234..7822
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7723..7742
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7954..8178
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8167..8184
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        8204..8222
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8204..8221
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gatggacctc tggccaggac tgctctcact ctcacgcacc aagaagctgc cattgatccc
       61 gctgctcagc agatactcag cggcattgcg ggacacaggc ccatggtacc aggagtgttt
      121 ctccagactg ttgactggcg tgatgtagtt gcttgggacc cagccttggc catttttggt
      181 ttgggcttca caccattccc cattgtgatt atagcctaag acccggagct tttcaccttt
      241 agttatgctt agagtgttat ctccactggc cacaaaatca tacagtgcaa cgaaaaggtt
      301 ggggtcattt tcactgggtc cagcgagaag gttttccttg gagttccaac gagcggcttc
      361 actcagaccc tgaggctcaa agtcagatgc tactggccgc tgaagggctt ttgaactctg
      421 cttaaatcca gtggctgagt ggacgatgac attcagaaac ccatagagcc ccggagactc
      481 atcatcttcc ttattgatgg tcagcggaat gctgtggaca gtctggagtt tcacacacga
      541 gttggtcagc atctgcagct ccacggatgt cagggagaag cttctgaaac acttcttctg
      601 ctgctcccgg atgttctccc tccactctgc acgctcatag tcagaggaga tcaggaacgt
      661 gtaactcttg ccgttgcggc tgtgcaccct gaaggccatg ctgggagaca taagcagcag
      721 cagtgactcc tgctccgaca gcttcttctt cagcctctcc gtagccttgc tgcccttgtt
      781 cgccctcttc tctctctgga tgtcactctt gatctgggag atcttgatct tcaaagcgtc
      841 cagctcctca tcgggcacca gggggatgtt gggcactgcc tccagttcat ccaccatctg
      901 gaagctgaga tccgtgagcg gaatgtacca tttgcagtca tactgctgcg ttttgcctcc
      961 gctctgcttc ttgagcttgg tgcagagaag caggtcggtg aacaggaaga cgtggcgcag
     1021 cttgcgggcc ccctccacca gctccaccat gaagctgtcc ttcagcagct gccggtgctc
     1081 tcccttcttc accgtcatgg actgccgtcg gggtgtgatc tcctcattga tgctggacag
     1141 gaagttctgt gagatgcgga gggcgtcctg cagcaagggg tggtcagggt ggctggcagg
     1201 agtgtgcttc agcaagtcat ggaggaccag cgtgctcctc gtcacacggt ccacaggctt
     1261 gtagagcaga gtttccagag agttcttggt cgttggatcc ttggcatctt tgttgcttct
     1321 ggctctcagg ttctcggaga tttctgcaaa ctgagcattg gcctgacagc acttctcagc
     1381 catttccatg gcaactccgt agttgtccac gaaggcccgg tacacaccca gctggctggc
     1441 cagcttctgg aagaggtcgc ccacccgctg ctggtggctc cactgctgca cgcgggggaa
     1501 gagcccatca tagaactcct tgtggatctc gtagagctca ggcactttga agaagatggt
     1561 ctcgatctgc tgactcgtca gcaccggctg agaggtggtg gcagcggctt tcaaaggctt
     1621 catgggcagc agcagtgcct ccaggtggct caggtaagtc tcctcgctag ccaggattcc
     1681 cgacaggacc cattttctca tctccaagcc cttttccaag tccagctcac tcgctttagt
     1741 ggactccagg gctcccgaga ccagcgcatc ccggctgccc ctgcccttgc tgctgaggtg
     1801 gggcgatgag gagggcgagt catcaatgta gggcagccca tcttggtgcc ggcgctgctc
     1861 ctctgcccgg tctgcagcgc agccgtatcc aggtggtgtt ccgaacgagc catctgcgtc
     1921 tccatggaag gcgccctcgc catcgttggg ccagatctgc ccggtcttgc ggacgcccac
     1981 gatggtggcc tcggacacga caaccgggca gtgccggtgc cgcttatggc actgcggcgt
     2041 ggggggactg ctgctgtcga aggactgttg cgagttctgc gagggagagc ggcttttgtc
     2101 ccggaacatg cggtaggtgg tggggcttgg ggacacgcgg ctggactggc cagaggagaa
     2161 gtcctcctcg ctggaggtga ggttctcatt ggagctgcag tccggggtat agccgcctcc
     2221 gcaatcctca aaactccggg gggagtagga cctgcggggc caggtaaggc gcttctcctg
     2281 ctcagaggtg ctctggctgc gcaggagcgg gcccttgccc tccccttcca tcatgccccc
     2341 gacgtagatg ctctggtagg gctggtactc caggggcggc caagggggcc tgctaccgcc
     2401 attggcgtcg atcaggttgt ccttcaggaa gcgggggttc aactcggcgt cctcgtagtc
     2461 gccgtcgacg ccgcagctgc tctccgagga gcgtccccgg taagggggcc tggatgcatc
     2521 ccccacgctc gagcccgcgc cgtgctggga ctttttgcgc tccatctgca tggcctggct
     2581 gcccagggag ctgatgcggt ccgacacctc tttgtcgttg accttcacca ggccgcgctc
     2641 gtggtgaaac tcgacgttca cgtagaaggg cttctcggcg tccgccccgg gctggccatg
     2701 gcccttgcgt atccgctcga agttggacct gagcgccgcc acgctggcgg ggggtccccg
     2761 gtcgtcccgt tcccccgacg cggctgcccc gggcctgcgg gcggtcccgg gcctggcctt
     2821 acccggagaa ccctcgccgt cgggccgggc ctcgggctcc tcggcgggcg gcgggtcggc
     2881 tccgtcggcg ggcgctggct gcgggcgcga cgcggacgct cggggctcgg aggcgccgtc
     2941 gggggcctgc gccgcgcgcc ggaagcccca tcgctgccgg tcatagctct tcttttcctt
     3001 ggccagcaac gtctgcaggt agatcatgcg gaagcgctcc tggttcacct cctgctccag
     3061 gcgccgaatg gaggccttgc agcgctccag ctcctgctcg atgtcgccca ctgagcgcag
     3121 ctccatgcgc gggggctctg agtccgggaa ctgcgccttc cacgcctccg cgaagcccac
     3181 cgggtccacc agatatctgc agaattcttt atcgtcatcg tctttgtaat ccatgttttg
     3241 attcccgggg gatccggtac caagcttggg tctccctata gtgagtcgta ttaatttcga
     3301 taagccagta agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc
     3361 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     3421 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     3481 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     3541 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     3601 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     3661 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     3721 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     3781 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     3841 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     3901 aactagtcaa taatcaatgt caacgcgcgc gtaaattgta agcgttaata ttttgttaaa
     3961 attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa
     4021 aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa
     4081 caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca
     4141 gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt cgaggtgccg
     4201 taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcc
     4261 ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta gggcgctggc
     4321 aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg cgccgctaca
     4381 gggcgcgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4441 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4501 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4561 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4621 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4681 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4741 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4801 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4861 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4921 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4981 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     5041 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     5101 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     5161 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     5221 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     5281 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     5341 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     5401 atatatactt tagattgatt tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct
     5461 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5521 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5581 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     5641 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5701 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5761 gtgaggaggc ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag
     5821 gatcgtttcg catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg
     5881 agaggctatt cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt
     5941 tccggctgtc agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc
     6001 tgaatgaact gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt
     6061 gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag
     6121 tgccggggca ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg
     6181 ctgatgcaat gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag
     6241 cgaaacatcg catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg
     6301 atctggacga agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga
     6361 gcatgcccga cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca
     6421 tggtggaaaa tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc
     6481 gctatcagga catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg
     6541 ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct
     6601 atcgccttct tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc
     6661 gacgcccaac ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg
     6721 cttcggaatc gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct
     6781 ggagttcttc gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga
     6841 aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt
     6901 tgttcataaa cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc
     6961 ccattggggc caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg
     7021 tgaaggccca gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta
     7081 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     7141 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     7201 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     7261 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     7321 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     7381 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     7441 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     7501 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     7561 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     7621 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     7681 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     7741 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     7801 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     7861 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     7921 tattaccgcc actggttctt tccgcctcag aagccataga gcccaccgca tccccagcat
     7981 gcctgctatt gtcttcccaa tcctccccct tgctgtcctg ccccacccca ccccccagaa
     8041 tagaatgaca cctactcaga caatgcgatg caatttcctc attttattag gaaaggacag
     8101 tgggagtggc accttccagg gtcaaggaag gcacggggga ggggcaaaca acagatggct
     8161 ggcaactaga aggcacagtc gaggctgatc agcgagctct agcatttagg tgacactata
     8221 gaatagggcc ctctagacta gtcatctact cgagcggccg cctaggcgat ccgctctgga
     8281 ggctggcggg ttttccgaag agacactcgg gttgatatga gagggatgaa ggcggtggaa
     8341 gacggctggt cccctgccag ggccgaggat gctgatggca acgtggaggg aacgggggct
     8401 gggctgatgg gggtccccga cggcttggcg gactgtggct ttggagtggc cgggagcacg
     8461 ggctttttga ggccctctcc cggctggctg ggcttggcag cgtcactgtt cacagcatca
     8521 accagactcg tggcttttgt ctttgcgccc aggactgcct ccccggccgc ctcctggctc
     8581 gggctctgcg agggctttcc tccagccttc cctgcagagg ctgctggtgg gggcggcggg
     8641 gcaggtttga gcctggacaa tttccccttg tccctccctg gcgactcaga ggagtgcttg
     8701 tgcctcctca ctctggactc ctcggcgggg cccttgctgg tgccccctgg tgcacctgag
     8761 cctgctttgc tggtgggggt cactggctca gctgcagcag gggtccctaa ggcactgccc
     8821 tttccagctt cttccttgtg ggggaggccc gaggcagggg ccacggtgac ctgccgccgg
     8881 aggggttttg gagtcaggtt gggcgggctg gagcccgggc tggactccat gatgtctttg
     8941 aagacctcat cagcagcttc ctcattcttt ttcaccagcc tggggggagg cgttactgtg
     9001 cctcgggtca cctggtcaga cctgttctcc cctgccctct tccgaggcag agccggcttc
     9061 tcacttttgt gccctccaaa tgtggacgag tcaaactgtc ttcccgtgga ctgcaagtcc
     9121 cgaggcagcg tgactgacct ccactccgtg tccttggccc catggggaac gcaggaggcg
     9181 gagcaagagc gcaggaagcg cttgctggag ctgccaccgc cctcctcctc gccggtggct
     9241 aggcggctgc tggtcagcgt gctggacttc ttccacaggt ggggagaccg gaagcctgag
     9301 cccccagact cccggagggc tccattgggg accccagccc cattgctggg ctttggggac
     9361 ttggctgggt cagctgtgtc caagggggtg aaagccagtg ccccgttgct gatgtctcgg
     9421 ccctcttcct cgccggcccc tctgcgctcc ggctggccgt ccatctcccg gaaggagctg
     9481 ctgcgtttgg gaggggttgg ggctgtcttc ttcttcttct tgatcaaggc gctgaacaag
     9541 ttggtctttt tgtctttggg gagaaggcgc tcatcttcat tcaggccgcc ctccggggga
     9601 cctcgctctt ttcgagggag caatggagac acggcaggct catggtccag aggatcgctc
     9661 tctccctggc ccttggagtg aggcatctca ggcacgtcag tggtgtctct gtgctctgca
     9721 gctctcctgg aggtcctcgt cttggtgggc agctctgggg cctgcagcaa ggtactcaca
     9781 gccccacgga cgccttgttt ccccagctcc ttttccactt cgtctgagat actggattcc
     9841 tggaacattg tttcaaaggc ttggtggatt tcagcaaagg agggccggtc agagggattc
     9901 cactgccaac atgctcgcat gagttcatag accttctctg ggcagccttc tgggcgctcc
     9961 atgcggtagt ccttctctag cagctcatac acctgggaca ggtcaattcc cgggtaaggg
    10021 gacatgccat aggtagcaat ttcccaaagc aatactccaa atgcccagac gtcggacttg
    10081 atggagaact tgttgtaggc caggctctcg ggtgcagtcc atttgatggg gaacttggct
    10141 ccagcatggg ctgtgtaggt gtcccctgtc atcaacctgc tcaggccaaa atcagctacc
    10201 ttcaccaagt ggttctcccc taccaggcag tttcgggcag caagatctct gtggatgaag
    10261 tttttcttct ccaggtactc catggctgac gagatctgag tggccatgta cagcagcacc
    10321 acggcgttca cctcctgccg gttgcactcc ctcaggtagt ccaggaggtt cccgtaggtc
    10381 atgaactcag tgatgatata gaacgggggc tcccgggtgc agaccccaag gagctgcacc
    10441 aggttagggt gtttgatctc tttcatgact gcagcttctt tcaagaactc ttccacctcc
    10501 atggtgtcct ccttcaaggt cttcacggcc accgtcaggc tgtatttctt ccacacgccc
    10561 tcgtacacct ccccgtactg gcccccgccc agcttgtgct tcatggtgat gtccgtgcgt
    10621 tccatctccc acttgtcgta gttgggggac acaccataga cagtgggctt gttgcgcttt
    10681 ggggctggat aatggagcgt ggtgatgagc ccgtcggcca ccgttgaatg atgatgaacc
    10741 aactcggcca gggtgttgaa gcggctctcg gaggagacgt agagcttgcc atcagaagca
    10801 gtgttgatcc tgtaatggta caccctccct tcgtatctca gcga
//
