LOCUS       sequence-407793-       10410 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of membrane-bound bPAC tagged with paGFP for 
            cAMP production.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Xiang KM, Park P, Koren SA, Hayward RF, Cohen AE
  TITLE     All-optical mapping of cAMP transport reveals rules of sub-cellular 
            localization
  JOURNAL   bioRxiv 2023
REFERENCE   2  (bases 1 to 10410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2023"
COMMENT     Sequence Label: pLenti-CMV-paGFP-bPAC-CAAX
FEATURES             Location/Qualifiers
     source          1..10410
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(47..66)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        238..617
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        619..817
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             835..1015
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1062..1187
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1680..1913
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2098..2142
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2440..2557
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2616..2919
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2920..3123
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     3120..3144
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             join(3206..3208,3209..3211,3212..3922)
                     /codon_start=1
                     /product="photoactivatable GFP variant (Patterson et al., 
                     2002)"
                     /label=PA-GFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSHQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3251..3272)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3512..3531)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3859..3880
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4994..5023
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     misc_feature    5102..5690
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5155..5175)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5573..5584)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(5693..5709)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5694..5710)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             6215..6395
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6421..6438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6427..6651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6697..7125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6784..6803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6994..7015
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7134..7154)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7139..7468
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7319..7454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7381..7400
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7516..7563
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7582..7956
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8086..8207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8123..8142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8177..8196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8256..8272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8256..8272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8269..8291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8280..8296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8304..8310,8311..8328,8329..8334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8349..8370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8487..8504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8658..9246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8738..8757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9417..10208,10209..10277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10040..10059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10278..10382)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
       61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
      121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
      181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
      241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
      301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
      361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
      421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
      481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
      541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
      601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
      661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
      721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
      781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
      841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
      901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
      961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
     2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2581 agatccagtt tggttaatta agcttgggag ttccgcgtta cataacttac ggtaaatggc
     2641 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
     2701 atagtaacgc caatagggac tttccattga cgtcaatggg tggagcattt acggtaaact
     2761 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
     2821 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
     2881 tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac
     2941 atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac
     3001 gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac
     3061 tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga
     3121 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
     3181 agaagacacc gactctagag gatccatggt gagcaagggc gaggagctgt tcaccggggt
     3241 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg
     3301 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg
     3361 caagctgccc gtgccctggc ccaccctcgt gaccaccttc agctacggcg tgcagtgctt
     3421 cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg
     3481 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga
     3541 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa
     3601 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta
     3661 tatcatggcc gacaagcaga agaacggcat caaggccaac ttcaagatcc gccacaacat
     3721 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg
     3781 ccccgtgctg ctgcccgaca accactacct gagccaccag tccaagctga gcaaagaccc
     3841 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct
     3901 cggcatggac gagctgtaca agggtggttc tggtatgaag cggctggtgt acatcagcaa
     3961 gatcagcggc cacctgagcc tggaagagat ccagcggatc ggcaaggtgt ccatcaagaa
     4021 caaccagcgg gacaacatca ccggcgtgct gctgtacctg cagggcctgt tcttccagat
     4081 cctggaaggc gagaacgaga aggtggacaa gctgtacaag aaaatcctgg tggacgaccg
     4141 gcacaccaac atcctgtgcc tgaaaaccga gtacgacatc accgaccgga tgttccccaa
     4201 ctgggccatg aaaaccatca acctgaacga gaacagcgag ctgatgatcc agcccatcaa
     4261 gagcctgctg cagaccatca cccagagcca ccgggtgctg gaaaagtaca tgcccgccag
     4321 agtgatctac ctgatcaacc agggcatcaa ccccctgacc gtggagcccc agctggtcga
     4381 gaagatcatc ttcttcagcg acatcctggc cttcagcacc ctgaccgaga agctgcccgt
     4441 gaacgaggtg gtcatcctgg tcaaccggta cttcagcatc tgcacccgga tcatcagcgc
     4501 ctacggcggc gaagtgacca agttcatcgg cgactgcgtg atggccagct tcaccaaaga
     4561 gcagggcgac gccgccatcc ggaccagcct ggacatcatc tccgagctga agcagctgcg
     4621 gcaccacgtg gaggccacca accccctgca cctgctgtac accggcatcg gcctgagcta
     4681 cggccacgtg atcgagggca acatgggcag cagcctgaag atggaccaca ccctgctggg
     4741 ggacgccgtg aacgtggccg ccaggctgga agccctgaca agacagctgc cctacgccct
     4801 ggcctttaca gccggcgtga agaagtgctg ccaggcccag tggaccttca tcaacctggg
     4861 agcccaccag gtcaagggca agcaggaagc catcgaggtc tacaccgtga atgaggccca
     4921 gaagtactac gacaccctgc agatcaccca gctgatccgg cagaccctgg aaaacgacaa
     4981 gccgcggacg tacgagcaaa agctgatctc ggaggaggac ctgaccggtg gtaaaaagaa
     5041 gaaaaagaag tcaaagacaa agtgtgtaat tatgtgagaa ttcgatatca agcttatcga
     5101 taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     5161 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
     5221 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     5281 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     5341 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     5401 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     5461 gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct
     5521 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     5581 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct
     5641 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg
     5701 tcgacctcga gacctagaaa aacatggagc aatcacaagt agcaatacag cagctaccaa
     5761 tgctgattgt gcctggctag aagcacaaga ggaggaggag gtgggttttc cagtcacacc
     5821 tcaggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     5881 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagata tccttgatct
     5941 gtggatctac cacacacaag gctacttccc tgattggcag aactacacac cagggccagg
     6001 gatcagatat ccactgacct ttggatggtg ctacaagcta gtaccagttg agcaagagaa
     6061 ggtagaagaa gccaatgaag gagagaacac ccgcttgtta caccctgtga gcctgcatgg
     6121 gatggatgac ccggagagag aagtattaga gtggaggttt gacagccgcc tagcatttca
     6181 tcacatggcc cgagagctgc atccggactg tactgggtct ctctggttag accagatctg
     6241 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     6301 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     6361 cagacccttt tagtcagtgt ggaaaatctc tagcagggcc cgtttaaacc cgctgatcag
     6421 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
     6481 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
     6541 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
     6601 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
     6661 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
     6721 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     6781 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     6841 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     6901 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     6961 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     7021 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     7081 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
     7141 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7201 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     7261 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     7321 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     7381 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
     7441 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
     7501 atctgatcag cacgtgttga caattaatca tcggcatagt atatcggcat agtataatac
     7561 gacaaggtga ggaactaaac catggccaag ttgaccagtg ccgttccggt gctcaccgcg
     7621 cgcgacgtcg ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc
     7681 gtggaggacg acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc
     7741 caggaccagg tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag
     7801 ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc
     7861 atgaccgaga tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc
     7921 aactgcgtgc acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc
     7981 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     8041 atcctccagc gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca
     8101 gcttataatg gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt
     8161 tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata
     8221 ccgtcgacct ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat
     8281 tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg
     8341 ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag
     8401 tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt
     8461 ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg
     8521 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     8581 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     8641 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     8701 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     8761 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     8821 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     8881 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     8941 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     9001 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     9061 ttcttgaagt ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct
     9121 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     9181 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     9241 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     9301 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat
     9361 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac
     9421 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt
     9481 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt
     9541 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag
     9601 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct
     9661 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt
     9721 gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc
     9781 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt
     9841 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg
     9901 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg
     9961 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct
    10021 tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa agtgctcatc
    10081 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt
    10141 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt
    10201 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg
    10261 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat
    10321 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg
    10381 cgcacatttc cccgaaaagt gccacctgac
//
