LOCUS       sequence-407808-       23440 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Baculoviral transfer vector to co-express 8 subunits of human augmin
            complex.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 23440)
  AUTHORS   Ustinova K, Ruhnow F, Gili M, Surrey T
  TITLE     Microtubule binding of the human augmin complex is directly 
            controlled by importins and Ran-GTP.
  JOURNAL   J Cell Sci. 2023 Jun 15;136(12):jcs261096. doi: 10.1242/jcs.261096. 
            Epub 2023 Jun 26.
  PUBMED    37357828
REFERENCE   2  (bases 1 to 23440)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2023-06-15"; volume: "136(12):jcs261096. doi"; pages: 
            " 10.1242/jcs.261096. Epub 2023 Jun 26"
COMMENT     Sequence Label: pBIG2abc_hsHAUScomplex_FL
FEATURES             Location/Qualifiers
     source          1..23440
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        303..405
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             406..1065
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(472..491)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      1365..1819
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1452..1471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1661..1682
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1845..1949
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1950..2018,2019..2810)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2168..2187)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2981..3569
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3470..3489
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3723..3740
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  3874..4098
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(4165..4698)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(4887..4892,4893..4909,4910..4915))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     protein_bind    complement(5311..5344)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     polyA_signal    5442..5490
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        5574..5665
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     CDS             6542..6565
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             6581..6598
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6736..6870
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        7019..7110
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    9099..9233
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    9568..9616
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        9700..9791
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     complement(10857..10876)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             11283..11303
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             join(11313..11318,11319..11342,11343..11378,11379..11402)
                     /codon_start=1
                     /product="two Strep-Tag(R)II moieties connected by a linker
                     for enhanced binding to Strep-Tactin(R), an engineered form
                     of streptavidin (Schmidt et al., 2013)"
                     /label=Twin-Strep-tag
                     /translation="SAWSHPQFEKGGGSGGGSGGSAWSHPQFEK"
     polyA_signal    11540..11674
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        11823..11914
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    14959..15093
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        15218..15309
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    16593..16727
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     polyA_signal    17062..17110
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        17194..17285
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    18554..18688
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        18837..18928
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    21007..21141
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        21266..21357
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     polyA_signal    22767..22901
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     mobile_element  23193..23358
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
ORIGIN
        1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga
       61 ctgaatatca acgcttattt gcagcctgaa ctgaacagga gggacagctg atagaaacag
      121 aagccactgg agcacctcaa aaacaccatc atacactaaa tcagtaagtt ggcagcatca
      181 cccgacgcac tttgcgccga ataaatacct gtgacggaag atcacttcgc agaataaata
      241 aatcctggtg tccctgttga taccgggaag ccctgggcca acttttggcg aaaatgagac
      301 gttgatcggc acgtaagagg ttccaacttt caccataatg aaataagatc actaccgggc
      361 gtattttttg agttatcgag attttcagga gctaaggaag ctaaaatgga gaaaaaaatc
      421 actggatata ccaccgttga tatatcccaa tggcatcgta aagaacattt tgaggcattt
      481 cagtcagttg ctcaatgtac ctataaccag accgttcagc tggatattac ggccttttta
      541 aagaccgtaa agaaaaataa gcacaagttt tatccggcct ttattcacat tcttgcccgc
      601 ctgatgaatg ctcatccgga attccgtatg gcaatgaaag acggtgagct ggtgatatgg
      661 gatagtgttc acccttgtta caccgttttc catgagcaaa ctgaaacgtt ttcatcgctc
      721 tggagtgaat accacgacga tttccggcag tttctacaca tatattcgca agatgtggcg
      781 tgttacggtg aaaacctggc ctatttccct aaagggttta ttgagaatat gtttttcgtc
      841 tcagccaatc cctgggtgag tttcaccagt tttgatttaa acgtggccaa tatggacaac
      901 ttcttcgccc ccgttttcac catgggcaaa tattatacgc aaggcgacaa ggtgctgatg
      961 ccgctggcga ttcaggttca tcatgccgtt tgtgatggct tccatgtcgg cagaatgctt
     1021 aatgaattac aacagtactg cgatgagtgg cagggcgggg cgtaattttt ttaaggcagt
     1081 tattggtgcc cttaaacgcc tggttgctac gcctgaataa gtgataataa gcggatgaat
     1141 ggcagaaatt cgaaagcaaa ttcgacccgg tcgtcggttc agggcagggt cgttaaatag
     1201 ccgcttatgt ctattgctgg tttaccggtt tattgactac cggaagcagt gtgaccgtgt
     1261 gcttctcaaa tgcctgaggc cagtttgctc aggctctccc cgtggaggta ataattgacg
     1321 atatgatcat ttattctgcc tcccagagcc tgatggcgaa tgggacgcgc cctgtagcgg
     1381 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     1441 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     1501 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     1561 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     1621 ggtttttcgc cctttgacgt tggagtccac gttcttaata gtggactctt gttccaaact
     1681 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     1741 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     1801 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     1861 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     1921 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     1981 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2041 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2101 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2161 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2221 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     2281 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     2341 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     2401 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     2461 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     2521 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     2581 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     2641 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     2701 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     2761 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     2821 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     2881 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     2941 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3001 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3061 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3121 atactattct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3181 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3241 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     3301 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     3361 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     3421 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     3481 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     3541 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     3601 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     3661 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     3721 gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc
     3781 atctgtgcgg tatttcacac cgcatagacc agccgcgtaa cctggcaaaa tcggttacgg
     3841 ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc ggacaataaa gtcttaaact
     3901 gaacaaaata gatctaaact atgacaataa agtcttaaac tagacagaat agttgtaaac
     3961 tgaaatcagt ccagttatgc tgtgaaaaag catactggac ttttgttatg gctaaagcaa
     4021 actcttcatt ttctgaagtg caaattgccc gtcgtattaa agaggggcgt ggccaagggc
     4081 atggtaaaga ctatattcgc ggcgttgtga caatttaccg aacaactccg cggccgggaa
     4141 gccgatctcg gcttgaacga attgttaggt ggcggtactt gggtcgatat caaagtgcat
     4201 cacttcttcc cgtatgccca actttgtata gagagccact gcgggatcgt caccgtaatc
     4261 tgcttgcacg tagatcacat aagcaccaag cgcgttggcc tcatgcttga ggagattgat
     4321 gagcgcggtg gcaatgccct gcctccggtg ctcgccggag actgcgagat catagatata
     4381 gatctcacta cgcggctgct caaacttggg cagaacgtaa gccgcgagag cgccaacaac
     4441 cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta ctacggagca agttcccgag
     4501 gtaatcggag tccggctgat gttgggagta ggtggctacg tctccgaact cacgaccgaa
     4561 aagatcaaga gcagcccgca tggatttgac ttggtcaggg ccgagcctac atgtgcgaat
     4621 gatgcccata cttgagccac ctaactttgt tttagggcga ctgccctgct gcgtaacatc
     4681 gttgctgctg cgtaacatcg ttgctgctcc ataacatcaa acatcgaccc acggcgtaac
     4741 gcgcttgctg cttggatgcc cgaggcatag actgtacaaa aaaacagtca taacaagcca
     4801 tgaaaaccgc cactgcgccg ttaccaccgc tgcgttcggt caaggttctg gaccagttgc
     4861 gtgagcgcat acgctacttg cattacagtt tacgaaccga acaggcttat gtcaactggg
     4921 tttgtgcctt catccgtttc cacggtgtgc gtcacccggc aaccttgggc agcagcgaag
     4981 tcgaggcatt tctgtcctgg ctggcgaacg agcgcaaggt ttcggtctcc acgcatcgtc
     5041 aggcattggc ggccttgctg ttcttctacg gcaaggtgct gtgcacggat ctgcccttgc
     5101 ttcaggagat cggtagacct cggccgtcgc ggcgcttgcc ggtggtgctg accccggatg
     5161 aagtggttcg catcctcggt tttctggaag gcgagcatcg tttgttcgcc caggactcta
     5221 gctatagttc tagtggttgg ctacagcttt gtttgtacta tcaacaggtt gaactgctga
     5281 tcaacagatc ctctacgcgg ccgcggtacc ataacttcgt atagcataca ttatacgaag
     5341 ttatctggcc taggttaatt aaacgctcta tggtctaaag tttttatcga ggctaactga
     5401 aacacggaag gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa
     5461 taaaacgcac gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc
     5521 actctgtcaa gtgaacgctc tatggtctaa agatttaaat gtatcgattc gagatcatgg
     5581 agataattaa aatgataacc atctcgcaaa taaataagta ttttactgtt ttcgtaacag
     5641 ttttgtaata aaaaaaccta taaatattcc ggattattca taccgtccca ccatcgggcg
     5701 cggatccatg gaacctcaag aggaacgtga aacccaggtg gccgcttggc tgaagaagat
     5761 tttcggcgat caccccattc cgcagtacga agtgaaccct cgtaccaccg aaatcctgca
     5821 ccacctgtct gagcgtaacc gtgtgcgtga ccgtgacgtg tacctggtca tcgaggacct
     5881 gaagcagaag gcttccgagt acgagtccga ggctaagtac ctgcaggacc tgctgatgga
     5941 atccgtgaac ttctcccctg ctaacctgtc ctccaccggt tctcgttacc tgaacgctct
     6001 ggtggactcc gctgtggctc tcgagactaa ggacacctct ctggctagct tcatccccgc
     6061 tgtgaacgac ctgacctctg acctgttccg taccaagtcc aagtccgagg aaatcaagat
     6121 cgagctggaa aagctcgaga agaacctgac cgctactctg gtgttggaga agtgcctgca
     6181 agaggacgtc aagaaggctg agctgcacct gtctaccgag cgtgctaagg tggacaaccg
     6241 tcgtcagaat atggacttcc tgaaggccaa gtctgaagag ttccgtttcg gtatcaaggc
     6301 tgctgaggaa cagctgtccg ctcgtggaat ggacgcttcc ctgtctcacc aatctctggt
     6361 ggctctgtcc gagaagctgg ctcgtctgaa gcagcagact atccctctca agaagaagct
     6421 cgagtcctac ctggacctga tgcctaatcc tagcctggct caagtgaaga tcgaggaagc
     6481 taagcgcgag ctggactcca tcgaggctga attgactcgt cgtgtggaca tgatggaact
     6541 gctcgaggtg ctgttccaag gtcctggtgg tggtggcggt caccaccatc accatcacta
     6601 ataaacaagc ttgtcgagaa gtactagagg atcataatca gccataccac atttgtagag
     6661 gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     6721 gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6781 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6841 ctcatcaatg tatcttatca tgtctggatc tgatcactgc ttgagcctag aagatccggc
     6901 tgctaacaaa gcccgaaagg aagctgagtt ggctgctgcc accgctgagc aataactatc
     6961 ataaccattt aaataaactg gatactattg cacgtttatt taaatgtatc gattcgagat
     7021 catggagata attaaaatga taaccatctc gcaaataaat aagtatttta ctgttttcgt
     7081 aacagttttg taataaaaaa acctataaat attccggatt attcataccg tcccaccatc
     7141 gggcgcggat ccatgtcttg tggaaacgag ttcgtggaaa ccctgaagaa gatcggttac
     7201 cccaaggctg acaacctgaa cggcgaggac ttcgactggc tgttcgaagg tgtcgaggac
     7261 gagtccttcc tgaagtggtt ctgcggcaac gtgaacgagc agaacgtgct gtccgagcgc
     7321 gagctggaag ctttctccat cctgcagaag tccggcaagc ccatcctgga aggtgctgct
     7381 ttggacgagg ctctcaagac ctgcaagacc tccgacctca agactccccg tctggacgac
     7441 aaagagctgg aaaagttgga ggacgaggtg cagaccctgc tgaagctgaa gaacctgaag
     7501 atccagcgtc gtaacaagtg ccagctgatg gcttccgtga cctctcacaa gtccctgcgt
     7561 ctgaacgcta aagaggaaga ggctaccaag aagctcaagc agtcccaggg tatcctgaac
     7621 gccatgatca ccaagatctc caacgagctg caggctctga ccgacgaagt gactcagctg
     7681 atgatgttct tccgtcactc caacctcggc cagggtacta accctctggt gttcctgtct
     7741 cagttcagcc tcgagaagta cctgtctcaa gaggaacagt ccaccgctgc tctgaccctg
     7801 tacactaaga agcagttctt ccagggcatc cacgaggtgg tcgagtcctc taacgaggac
     7861 aacttccagc tgctggacat ccagactcca tccatctgcg acaatcaaga gatcctcgaa
     7921 gaacgtcgtc tggaaatggc tcgtctgcag ctggcttaca tctgcgctca gcaccagctg
     7981 atccacctga aggcttccaa ctcctccatg aagtcctcca tcaagtgggc tgaagagtcc
     8041 ctgcactccc tgacctccaa ggctgtggac aaagagaacc tggacgctaa gatcagctct
     8101 ctgacctctg agatcatgaa gctcgagaaa gaagtgaccc agatcaagga ccgttctctg
     8161 cccgctgtcg tgcgtgaaaa cgctcaactg ctgaacatgc ccgtggtcaa gggcgacttc
     8221 gacctgcaga tcgctaagca ggactactac accgctcgtc aagagctggt cctgaaccag
     8281 ttgatcaagc agaaggcctc cttcgagctg ctgcagctgt cctacgagat cgagctgagg
     8341 aagcaccgcg acatctaccg tcagctcgaa aacctggtgc aagagctgtc ccagtccaac
     8401 atgatgctgt acaagcagtt ggagatgctg actgacccct ccgtgtctca gcagatcaac
     8461 cctcgtaaca ccatcgacac caaggactac tctacccacc gtctgtacca ggtgctcgag
     8521 ggcgagaaca agaagaaaga gctgttcttg acccacggca acctggaaga ggtggccgaa
     8581 aagctgaagc agaacatctc cctggtccaa gaccagctgg ctgtgtccgc tcaagagcac
     8641 tcattctttc tgtccaagcg taacaaggac gtggacatgc tgtgcgacac cctgtaccaa
     8701 ggtggcaacc agctgctcct gtctgaccaa gagctgaccg agcagttcca caaggtggaa
     8761 tcccagctga acaagctgaa ccacctcctg accgacatcc tggctgacgt caagaccaag
     8821 cgcaagaccc tggctaacaa caagctccac cagatggaac gcgagttcta cgtgtacttc
     8881 ctcaaggacg aggactacct gaaggacatc gtcgagaacc tcgagactca gtccaagatc
     8941 aaggctgtct ccctcgagga ctaataaaca agcttgtcga gaagtactag aggatcataa
     9001 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc
     9061 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata
     9121 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     9181 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg atctgatcac
     9241 tgcttgagcc tagaagatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct
     9301 gccacattta aataaatcta tatctcaatc ggggttacgt cgatacccca ccgagacccc
     9361 attgggacca atacgcccgc gtttcttcct tttccccacc ccaaccccca agttcgggtg
     9421 aaggcccagg gctcgcagcc aacgtcgggg cggcaagccc tgccatagcc actacgggtt
     9481 aattaaaaac tatatctcaa tcggggtttt tatcgaggct aactgaaaca cggaaggaga
     9541 caataccgga aggaacccgc gctatgacgg caataaaaag acagaataaa acgcacgggt
     9601 gttgggtcgt ttgttcataa acgcggggtt cggtcccagg gctggcactc tgtcaagtga
     9661 acgctctatg gtctaaagat ttaaatgtat cgattcgaga tcatggagat aattaaaatg
     9721 ataaccatct cgcaaataaa taagtatttt actgttttcg taacagtttt gtaataaaaa
     9781 aacctataaa tattccggat tattcatacc gtcccaccat cgggcgcgga tccatggctg
     9841 ctgctaatcc ttgggatccc gcttctgctc ctaacggtgc tggactggtg ctgggccatt
     9901 tcattgcttc cggcatggtc aatcaagaga tgctgaacat gtccaagaaa accgtgtcct
     9961 gcttcgtgaa cttcacccgt ctgcagcaga tcaccaacat ccaggctgag atctaccaga
    10021 agaacctcga gatcgagctg ctgaagctcg agaaggacac tgctgacgtg gtgcacccat
    10081 tctttctggc tcagaagtgc cacactctgc agtccatgaa caaccacctc gaggctgtgc
    10141 tgaaagagaa gcgttctctg cgtcagcgtc tgctcaagcc catgtgccaa gagaacctgc
    10201 ctatcgaggc cgtgtaccac cgttacatgg tgcacctgtt ggagctggct gtgaccttca
    10261 tcgagcgtct ggaaacccac ctggaaacca tccgtaacat ccctcacctg gctgctaacc
    10321 tgaagaagat gaaccaggct ctggctaaga tggacatcct ggtcaccgag actgaggaac
    10381 tggctgagaa catcctgaag tggcgcaagc agcagaacga ggtgtcctct tgcatcccca
    10441 agatcctggc tgaggaatcc tacctgtaca agcacgacat catcatgcct ccactgcctt
    10501 tcacctccaa ggtgcacgtc cagaccatca acgctaaagg tggtggtggc ggtgtctcca
    10561 agggcgaaga actgttcact ggtgtcgtgc ctatcctggt ggaactggat ggcgacgtga
    10621 acggtcacaa gttctctgtg tctggcgaag gcgagggcga cgctacttac ggaaagctga
    10681 ccctgaagtt catctgcacc accggcaagt tgcccgtgcc ttggcctact ctggtcacca
    10741 ctctgactta cggtgtccag tgcttctctc gttaccccga ccacatgaag cagcacgatt
    10801 tcttcaagtc cgctatgccc gagggttacg tgcaagagcg taccatcttc tttaaggacg
    10861 acggcaacta caagacccgc gctgaagtga agttcgaagg cgacaccctg gtcaaccgta
    10921 tcgagctgaa gggtatcgac ttcaaagagg acggcaacat cctgggccac aagctcgagt
    10981 acaactacaa ctcccacaac gtgtacatca tggccgacaa gcaaaagaac ggcatcaaag
    11041 tcaacttcaa gatccgccac aacatcgagg acggttccgt gcagttggct gaccactacc
    11101 agcagaacac tcctatcggc gacggtcctg tgctgctgcc tgacaaccac tacctgtcta
    11161 cccagtccgc tctgtctaag gaccccaacg agaagcgtga ccacatggtc ctgaaagagt
    11221 tcgtcaccgc tgctggtatc accctcggaa tggacgagct gtacaaaggt ggcggaggtg
    11281 gcgagaacct gtactttcaa ggcatggctg cctccgcttg gtcacaccct cagttcgaaa
    11341 aaggcggtgg ttccggtggt ggttctggcg gttctgcttg gagtcaccca caattcgaga
    11401 agtaataaac aagcttgtcg agaagtacta gaggatcata atcagccata ccacatttgt
    11461 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
    11521 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
    11581 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
    11641 caaactcatc aatgtatctt atcatgtctg gatctgatca ctgcttgagc ctagaagatc
    11701 cggctgctaa caaagcccga aaggaagctg agttggctgc tgccaccgct gagcaataac
    11761 tatcataacc atttaaataa actggatact attgcacgtt tatttaaatg tatcgattcg
    11821 agatcatgga gataattaaa atgataacca tctcgcaaat aaataagtat tttactgttt
    11881 tcgtaacagt tttgtaataa aaaaacctat aaatattccg gattattcat accgtcccac
    11941 catcgggcgc ggatccatgt catctgcttc cgtgaccgct ttcgagaaag aacacctgtg
    12001 gatgtacctg caggctctgg gtttcgagcc cggtcctgct actatcgctt gcggcaagat
    12061 cgtgtctcac acccacctgg gtgttaacat gttcgacaag ctgaaccgcg acgctttcca
    12121 catcatcagc tactttctgt tccaagtgct ggaccagtct ctgaccaaag aggtgttcaa
    12181 gttctgctgg cctccattcg accagaagtc cgacactgag ttccgcaagc actgctgcga
    12241 gtggatcaag cgtatcagcg gagagtgcgg ttctagcttc cctcaggtcg tgggttccct
    12301 gttcttgtcc cctggtggtc ccaagttcat ccacctgatg taccacttcg ctcgcttcgt
    12361 ggctatgaag tacatcaagt ccaactccaa gaactccagc caccacttcg tggaaacctt
    12421 caacatcaag ccccaggacc tgcacaagtg tatcgctcgt tgccacttcg cccgttctcg
    12481 tttcctgcag atcctgcagc gtcaggactg cgtgacccag aagtaccaag agaacgctca
    12541 gctgtccgtg aagcaagtgc gcaacctgcg ttccgagtgc atcggcctgg aaaaccagat
    12601 caagaagatg gaaccttacg acgaccactc caacatggaa gagaagatcc agaaagtgcg
    12661 ctccctgtgg gcttccgtca acgagactct gatgttcctg gaaaaagaac gcgaggtggt
    12721 gtcctccgtg ctgtcccttg ttaaccagta cgctctggac ggcaccaacg tggccatcaa
    12781 catccctcgt ctgctgctgg acaagatcga aaagcagatg ttccagctgc acatcggcaa
    12841 cgtgtacgag gctggcaagt tgaacctgct gaccgtgatc cagctgctga acgaggtgct
    12901 gaaagtgatg aagtacgagc gttgccaggc tgaccaggct cgtttgactg tcgacctgca
    12961 ctacttggag aaagagacta agttccagaa agagcgtctg tccgacctga agcacatgcg
    13021 ttaccgtatc aaggacgacc tgaccaccat ccgtcactcc gtggtggaaa agcaaggcga
    13081 gtggcacaag aagtggaaag agttcctggg actgtccccg ttctctctga tcaaaggatg
    13141 gaccccttcc gtggacctgc tgcctcctat gtctcctctg tctttcgacc ccgcttccga
    13201 ggaagtgtac gctaagtcca tcctgtgtca gtaccctgct tctctgcccg acgctcacaa
    13261 gcagcacaat caagaaaacg gttgccgtgg cgactctgac actttgggtg ctctgcacga
    13321 cctggctaac tctcccgctt cattcctgtc tcagtccgtg tcctcctccg accgtaactc
    13381 tgtgaccgtg ctcgagaagg acaccaagat gggcacccct aaagagaaga acgaggccat
    13441 ctccaagaag atccccgagt tcgaggtcga gaactcccct ctgtccgatg tggctaagaa
    13501 caccgagtcc tccgctttcg gtggttccct gcctgctaag aagtctgacc cattccaaaa
    13561 agagcaggac cacctggtcg aagaggtggc cagagctgtg ctgtctgact cccctcaact
    13621 gtccgagggc aaagagatca agctcgagga actgatcgac tccctgggtt ctaacccctt
    13681 cctgactcgt aaccagattc ctcgtactcc cgagaacctg attaccgaga tccgttcctc
    13741 ctggcgcaag gctatcgaga tggaagaaaa ccgtaccaaa gaacccatcc agatggacgc
    13801 tgagcaccgt gaagtgctgc ctgaatctct gcctgtgctg cacaaccagc gcgagttctc
    13861 tatggctgac ttcctgctgg aaaccaccgt gtccgacttc ggtcaatccc acctgaccga
    13921 ggaaaaagtg atctccgact gcgagtgcgt gccacagaag cacgtcctga cttctcacat
    13981 cgacgagcct ccaactcaga accagtctga cctgctgaac aagaaagtca tctgcaagca
    14041 ggacctcgag tgcctggctt tcaccaagct gtccgagact tcccgtatgg aaactttctc
    14101 ccctgctgtg ggcaaccgta tcgacgtgat gggaggttcc gaagaggaat tcatgaagat
    14161 cctggaccac ttggaggtgt cctgcaacaa gccctccacc aacaagacta tgctgtggaa
    14221 ctccttccag atctcctccg gtatctcctc caagtccttc aaggacaacg acttcggcat
    14281 cctgcacgaa accctgcctg aggaagtcgg tcacctgtcc ttcaactcct ccagctcctc
    14341 tgaggctaac ttcaagctgg aacccaactc tcccatgcac ggtggtactc tgttggagga
    14401 cgttgtcggt ggtcgtcaga ccactcctga gtctgacttc aacctccaag ctctgcgttc
    14461 ccgttacgag gctctgaaga agtccctgtc caagaagcgt gaagagtcct acctgtccaa
    14521 ctctcagacc cctgagcgtc acaagcctga gctgtcacct actcctcaga acgtgcagac
    14581 cgacgatacc ctgaacttcc tggatacctg cgacctccac accgagcaca ttaagccttc
    14641 tctgcgcacc tccatcggcg agagaaagcg ttcactgtcc ccactgatca agttctcccc
    14701 agtcgagcag cgtctgcgta ccactattgc ttgctccctg ggagagctgc ccaacctgaa
    14761 agaagaggac atcctgaaca agtcgctgga cgctaaagag cctccttccg acttgacccg
    14821 ttaataaaca agcttgtcga gaagtactag aggatcataa tcagccatac cacatttgta
    14881 gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa acataaaatg
    14941 aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa ataaagcaat
    15001 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
    15061 aaactcatca atgtatctta tcatgtctgg atctgatcac tgcttgagcc tagaagatcc
    15121 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccacattta aataaaccta
    15181 atgatgcctg atgtttattt aaatgtatcg attcgagatc atggagataa ttaaaatgat
    15241 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa
    15301 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc catgggtggt
    15361 gctagattgg gtgctcgtaa catggctgga caggacgctg gttgtggtag aggtggcgac
    15421 gactactctg aggacgaagg cgactcttcc gtgtctcgtg ctgctgtcga agtgttcggc
    15481 aagctgaagg acctgaactg cccattcctc gagggcctgt acatcaccga gcctaagacc
    15541 atccaagagc tgctgtgctc cccatccgag taccgtctgg aaatcctcga gtggatgtgc
    15601 acccgtgtgt ggccttctct gcaggaccgt ttctcatccc tgaagggtgt ccccaccgaa
    15661 gtgaagatcc aagagatgac caagctgggt cacgagctga tgttgtgcgc tcccgacgac
    15721 caagaactgc tgaagggttg cgcttgcgct caaaagcagc tgcacttcat ggaccagctg
    15781 ctggacacca tccgttctct gaccatcggt tgctcctctt gctccagcct gatggaacat
    15841 ttcgaggaca cccgcgagaa gaacgaggct ctgctgggag aactgttcag ctcccctcac
    15901 ctccagatgc tgctgaaccc tgaatgcgac ccttggcctc tggacatgca gcctctgctg
    15961 aacaagcagt ctgacgactg gcagtgggct tccgcttccg ctaagtccga ggaagaggaa
    16021 aagctggctg agctggctcg tcagctgcaa gagtctgctg ctaagctgca cgctctgcgt
    16081 accgagtact tcgctcagca cgaacaaggt gctgctgctg gcgctgctga catctctact
    16141 ctggaccaaa agctgcgtct ggtcacctcc gacttccacc aactgatcct ggctttcctg
    16201 caagtgtacg acgacgagct gggagagtgc tgtcaacgtc ctggtcctga cttgcacccc
    16261 tgcggtccta tcatccaggc tacccaccag aacctgacct cttactctca gctgctgcag
    16321 gtcgtgatgg ctgtggctga cacttctgct aaggctgtgg aaaccgtgaa gaagcagcag
    16381 ggcgaacaga tctgctgggg tggtagctcc tctgtgatgt ccctggctac caagatgaac
    16441 gaactgatgg aaaagtaata aacaagcttg tcgagaagta ctagaggatc ataatcagcc
    16501 ataccacatt tgtagaggtt ttacttgctt taaaaaacct cccacacctc cccctgaacc
    16561 tgaaacataa aatgaatgca attgttgttg ttaacttgtt tattgcagct tataatggtt
    16621 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
    16681 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctggatctga tcactgcttg
    16741 agcctagaag atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccaca
    16801 tttaaataaa tctatatctc aatcggggtt acgtcgatac cccaccgaga ccccattggg
    16861 accaatacgc ccgcgtttct tccttttccc caccccaacc cccaagttcg ggtgaaggcc
    16921 cagggctcgc agccaacgtc ggggcggcaa gccctgccat agccactacg ggttaattaa
    16981 aaactggata ctattgcacg tttttatcga ggctaactga aacacggaag gagacaatac
    17041 cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac gggtgttggg
    17101 tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcaa gtgaacgctc
    17161 tatggtctaa agatttaaat gtatcgattc gagatcatgg agataattaa aatgataacc
    17221 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta
    17281 taaatattcc ggattattca taccgtccca ccatcgggcg cggatccatg gcttctggcg
    17341 atttctgttc tcccggcgaa ggcatggaaa tcctgcagca agtgtgctcc aagcagctgc
    17401 ctccttgcaa cctgtccaaa gaggacctgc tgcagaaccc ctacttctct aagctgctgc
    17461 tgaacctgtc tcagcacgtg gacgagtctg gactgtctct gaccctggct aaagagcagg
    17521 ctcaggcttg gaaagaagtg cgtctgcaca agaccacctg gctgcgttct gagatcctgc
    17581 accgtgtcat ccaagagctg ctggtggact actacgtgaa gatccaggac accaacgtga
    17641 cctccgagga caagaagttc cacgagactc tcgagcagcg tctgctggtc actgaactga
    17701 tgaggctgct gggaccttct caagagcgtg aaatccctcc tctgctgggc ctcgaaaagg
    17761 ctgacctgct ggaactgatg cccctgtctg aggacttcgt gtggatgcgt gctcgcctgc
    17821 aacaagaggt cgaggaacag ctgaagaaga agtgcttcac cctgctgtgc tactacgacc
    17881 ccaactccga cgctgactcc gagactgtga aggctgctaa agtgtggaag ctggctgagg
    17941 tgctcgtggg agaacaacag cagtgccagg acgctaagtc tcagcagaaa gaacagatgc
    18001 tgctcctcga gaagaagtcc gctgcttact cccaagtgct gctgcgttgc ctgactctgc
    18061 tgcagcgttt gctgcaagag cacaggctca agacccagtc cgagctggac cgtatcaacg
    18121 ctcagtacct ggaagtgaag tgcggtgcta tgatcctgaa gctgcgtatg gaagaactga
    18181 agatcctgtc cgacacctac accgtcgaga aggtggaagt gcaccgtctg atccgtgacc
    18241 gtttggaagg tgctatccac ctccaagagc aggacatgga aaactcccgt caggtgctga
    18301 actcctacga ggtgctggga gaagagttcg accgtctggt caaagagtac accgtcctga
    18361 agcaggctac cgagaacaag cgttgggctc tgcaagagtt ctccaaggtg taccgttaat
    18421 aaacaagctt gtcgagaagt actagaggat cataatcagc cataccacat ttgtagaggt
    18481 tttacttgct ttaaaaaacc tcccacacct ccccctgaac ctgaaacata aaatgaatgc
    18541 aattgttgtt gttaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
    18601 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
    18661 catcaatgta tcttatcatg tctggatctg atcactgctt gagcctagaa gatccggctg
    18721 ctaacaaagc ccgaaaggaa gctgagttgg ctgctgccac cgctgagcaa taactatcat
    18781 aaccatttaa ataaactgga tactattgca cgtttattta aatgtatcga ttcgagatca
    18841 tggagataat taaaatgata accatctcgc aaataaataa gtattttact gttttcgtaa
    18901 cagttttgta ataaaaaaac ctataaatat tccggattat tcataccgtc ccaccatcgg
    18961 gcgcggatcc atggaattgg ctcaagaggc tcgtgaactc ggttgctggg ctgtcgaaga
    19021 aatgggtgtt cctgtggctg ctcgtgctcc tgagtctact ttgcgtcgtc tgtgcctcgg
    19081 acagggtgct gacatctggg cttacatcct gcagcacgtg cactcccagc gtaccgttaa
    19141 gaagatccgc ggcaacctgc tttggtacgg tcaccaggat tctccccaag tgcgccgcaa
    19201 gctggaactg gaagctgctg ttactcgtct gcgtgctgag atccaagagc tggaccagtc
    19261 cttggagctg atggaacgtg acaccgaggc tcaggacacc gctatggaac aggctcgtca
    19321 gcacacccag gacactcaac gtcgtgctct gctgttgcgt gctcaagctg gtgctatgcg
    19381 tcgtcagcaa cacaccctgc gtgaccctat gcagcgtctg caaaaccagc tgcgtcgcct
    19441 gcaagacatg gaacgcaagg ctaaggtgga cgtgaccttc ggttctctga cctctgctgc
    19501 tctgggactc gaacctgtgg tgttgcgtga tgtgcgtacc gcttgtaccc tgagggctca
    19561 gttcctgcag aacctgctgc tgcctcaggc taagaggggt tctctgccta ctcctcacga
    19621 cgaccacttc ggcacctctt accagcaatg gctgtcctcc gtggaaaccc tgctgaccaa
    19681 ccatcctcct ggtcacgtgc tggctgcttt ggaacatctg gctgctgagc gcgaggctga
    19741 aatccgttct ttgtgctctg gcgacggact gggcgacact gagatttcta gaccccaagc
    19801 tcctgaccag tccgactctt ctcagaccct gccttccatg gtgcacctca tccaagaagg
    19861 atggcgtacc gtcggagtgc tggtgtctca acgttccact ctgctgaaag agcgtcaggt
    19921 cctgactcag agactgcagg gactcgtgga agaggtggaa cgtcgtgtgc tgggatcctc
    19981 tgagcgtcaa gtgctgatcc tgggactgcg taggtgctgc ctgtggactg aactgaaggc
    20041 tctgcacgac cagtctcaag agctgcaaga tgctgctggt caccgtcagc tgttgctgcg
    20101 tgaactgcag gctaagcagc agcgtatcct gcactggcgt cagctggtgg aagagactca
    20161 agagcaagtc cgtctgctga tcaagggcaa ctccgcttcc aagaccaggt tgtgcagatc
    20221 tcctggcgaa gtgctggctc tggtgcagag aaaggtggtg cctactttcg aggctgtggc
    20281 tcctcagtct agagagctgc tgcgttgcct cgaggaagaa gtccgtcact tgcctcacat
    20341 cctgctgggt actctgttgc gtcaccgtcc tggcgagctg aagcctttgc ctactgtgct
    20401 gccctccatc caccaattgc atcctgcttc tccccgtggt tcctccttca ttgctctgtc
    20461 ccacaagctg ggcctgccac ctggaaaagc ttccgagttg ttgctgcctg ctgccgcttc
    20521 tctgcgtcaa gacttgctgt tgttgcagga ccagcgttcc ctgtggtgct gggacttgct
    20581 gcacatgaag acttccctgc caccaggact gcccactcaa gaactgctgc agatccaggc
    20641 ttcccaagag aagcagcaga aagagaacct gggtcaagct ctgaagcgcc tcgagaagct
    20701 gctcaagcag gctttggagc gtatccccga gctgcagggt atcgtcggag attggtggga
    20761 acagcctgga caggctgctc tgtctgaaga actgtgccag ggactgtctc tgcctcaatg
    20821 gcgtttgcgt tgggtgcaag ctcagggtgc tctgcagaag ctgtgctcct aataaacaag
    20881 cttgtcgaga agtactagag gatcataatc agccatacca catttgtaga ggttttactt
    20941 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattgtt
    21001 gttgttaact tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat
    21061 ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat
    21121 gtatcttatc atgtctggat ctgatcactg cttgagccta gaagatccgg ctgctaacaa
    21181 agcccgaaag gaagctgagt tggctgctgc cacatttaaa taaacctaat gatgcctgat
    21241 gtttatttaa atgtatcgat tcgagatcat ggagataatt aaaatgataa ccatctcgca
    21301 aataaataag tattttactg ttttcgtaac agttttgtaa taaaaaaacc tataaatatt
    21361 ccggattatt cataccgtcc caccatcggg cgcggatcca tggctgattc atctggtaga
    21421 ggtgctggca agcctgctac cggtcctacc aactcatcct ccgctaagaa gaaggacaag
    21481 cgcgtccaag gtggtcgtgt gatcgagtcc cgttacctgc agtacgagaa gaaaaccact
    21541 cagaaggctc ccgctggcga cggttctcag actcgtggaa aaatgtccga aggtggccgc
    21601 aagtcctctc tgctgcagaa gtctaaggct gactcctccg gtgttggaaa gggcgacctg
    21661 cagtctactc tgctggaagg acatggaacc gctcctccag acctggacct gtccgctatc
    21721 aacgacaagt ccatcgtgaa aaagacccct cagctggcta agaccatctc caagaagccc
    21781 gagtccacct cattctccgc tcctaggaag aagtcccctg acctgtctga ggctatggaa
    21841 atgatggaat ctcagaccct gctgctgact ctgctgtccg tgaagatgga aaacaacctg
    21901 gctgagttcg agcgtcgtgc tgagaagaac ctgctgatca tgtgcaaaga gaaagagaag
    21961 ctccagaaga aggctcacga gctgaagcgt cgtctgctgt tgtcccagag gaagcgtgaa
    22021 ctggctgacg tgctggacgc tcagatcgag atgctgtcac ctttcgaggc tgtggctacc
    22081 cgtttcaaag agcagtaccg taccttcgct accgctctgg acaccactcg tcacgaactg
    22141 cctgtgcgtt ctatccacct ggaaggcgac ggtcaacagc tgctggatgc tctgcaacac
    22201 gagctggtca ctactcagcg tctgctggga gaactggacg tgggagactc tgaggaaaac
    22261 gtgcaggttc tggacctgct gtctgagctg aaggacgtga ccgccaagaa ggacctggaa
    22321 ttgcgtcgta gcttcgctca ggtgctggaa ctgtctgctg aggcttccaa agaggctgct
    22381 ctggctaatc aagaagtgtg ggaagagact caaggcatgg ctcctccatc tcgttggtac
    22441 ttcaaccagg actccgcttg ccgtgaatcc ggtggtgctc ctaagaacac tcctctgtcc
    22501 gaggacgaca accctggtgc ttcttctgct cctgctcagg ctaccttcat cagcccttcc
    22561 gaggacttct ccagctcatc ccaagctgag gtgccacctt ctctgtcccg ttccggtcgt
    22621 gacctgtcct aataaacaag cttgtcgaga agtactagag gatcataatc agccatacca
    22681 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
    22741 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
    22801 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
    22861 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta
    22921 gaagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc cacatttaaa
    22981 taaatctata tctcaatcgg ggttacgtcg ataccccacc gagaccccat tgggaccaat
    23041 acgcccgcgt ttcttccttt tccccacccc aacccccaag ttcgggtgaa ggcccagggc
    23101 tcgcagccaa cgtcggggcg gcaagccctg ccatagccac tacgggttaa ttaaaaacct
    23161 aatgatgcct gatgtttcaa tcaggagatc cgaaccagat aagtgaaatc tagttccaaa
    23221 ctattttgtc atttttaatt ttcgtattag cttacgacgc tacacccagt tcccatctat
    23281 tttgtcactc ttccctaaat aatccttaaa aactccattt ccacccctcc cagttcccaa
    23341 ctattttgtc cgcccacagc ggggcatttt tcttcctgtt atgtttttaa tcaaacatcc
    23401 tgccaactcc atgtgacaaa ccgtcatctt cggctacttt
//
