LOCUS       sequence-407820-       10670 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BCR-ABL oncogene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10670)
  AUTHORS   Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky 
            NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek 
            L, Krejci P
  TITLE     Elucidation of protein interactions necessary for the maintenance of
            the BCR-ABL signaling complex.
  JOURNAL   Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. doi: 
            10.1007/s00018-019-03397-7. Epub 2019 Dec 9.
  PUBMED    31820037
REFERENCE   2  (bases 1 to 10670)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-019-03397-7"; journalName: "Cell Mol Life Sci"; 
            date: "2020-10"; volume: "77"; issue: "19"; pages: "3885-3903"
COMMENT     Sequence Label: pcDNA3.1-BCR-ABL-p210-Delta-IDR3-FLAG
FEATURES             Location/Qualifiers
     source          1..10670
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(3121..3144)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(3187..3206)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3188..3206)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(3251..3454)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(3280..3300)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        3455..3834
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     rep_origin      complement(3846..4301)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3983..4004)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     4195..4214
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        4328..4432
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4433..4501,4502..5293)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4651..4670)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        5353..5710
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     enhancer        5353..5544
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     complement(5376..5396)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      5561..5696
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             5745..6539
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             5745..6539
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5799..5818)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6409..6428
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(6727..6746)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    6771..6818
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     rep_origin      7147..7735
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7636..7655
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    7867..8091
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     8080..8097
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        8117..8135
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     8117..8134
                     /label=SP6
                     /note="SP6 promoter, forward primer"
ORIGIN
        1 gcgggacaca ggcccatggt accaggagtg tttctccaga ctgttgactg gcgtgatgta
       61 gttgcttggg acccagcctt ggccattttt ggtttgggct tcacaccatt ccccattgtg
      121 attatagcct aagacccgga gcttttcacc tttagttatg cttagagtgt tatctccact
      181 ggccacaaaa tcatacagtg caacgaaaag gttggggtca ttttcactgg gtccagcgag
      241 aaggttttcc ttggagttcc aacgagcggc ttcactcaga ccctgaggct caaagtcaga
      301 tgctactggc cgctgaaggg cttttgaact ctgcttaaat ccagtggctg agtggacgat
      361 gacattcaga aacccataga gccccggaga ctcatcatct tccttattga tggtcagcgg
      421 aatgctgtgg acagtctgga gtttcacaca cgagttggtc agcatctgca gctccacgga
      481 tgtcagggag aagcttctga aacacttctt ctgctgctcc cggatgttct ccctccactc
      541 tgcacgctca tagtcagagg agatcaggaa cgtgtaactc ttgccgttgc ggctgtgcac
      601 cctgaaggcc atgctgggag acataagcag cagcagtgac tcctgctccg acagcttctt
      661 cttcagcctc tccgtagcct tgctgccctt gttcgccctc ttctctctct ggatgtcact
      721 cttgatctgg gagatcttga tcttcaaagc gtccagctcc tcatcgggca ccagggggat
      781 gttgggcact gcctccagtt catccaccat ctggaagctg agatccgtga gcggaatgta
      841 ccatttgcag tcatactgct gcgttttgcc tccgctctgc ttcttgagct tggtgcagag
      901 aagcaggtcg gtgaacagga agacgtggcg cagcttgcgg gccccctcca ccagctccac
      961 catgaagctg tccttcagca gctgccggtg ctctcccttc ttcaccgtca tggactgccg
     1021 tcggggtgtg atctcctcat tgatgctgga caggaagttc tgtgagatgc ggagggcgtc
     1081 ctgcagcaag gggtggtcag ggtggctggc aggagtgtgc ttcagcaagt catggaggac
     1141 cagcgtgctc ctcgtcacac ggtccacagg cttgtagagc agagtttcca gagagttctt
     1201 ggtcgttgga tccttggcat ctttgttgct tctggctctc aggttctcgg agatttctgc
     1261 aaactgagca ttggcctgac agcacttctc agccatttcc atggcaactc cgtagttgtc
     1321 cacgaaggcc cggtacacac ccagctggct ggccagcttc tggaagaggt cgcccacccg
     1381 ctgctggtgg ctccactgct gcacgcgggg gaagagccca tcatagaact ccttgtggat
     1441 ctcgtagagc tcaggcactt tgaagaagat ggtctcgatc tgctgactcg tcagcaccgg
     1501 ctgagaggtg gtggcagcgg ctttcaaagg cttcatgggc agcagcagtg cctccaggtg
     1561 gctcaggtaa gtctcctcgc tagccaggat tcccgacagg acccattttc tcatctccaa
     1621 gcccttttcc aagtccagct cactcgcttt agtggactcc agggctcccg agaccagcgc
     1681 atcccggctg cccctgccct tgctgctgag gtggggcgat gaggagggcg agtcatcaat
     1741 gtagggcagc ccatcttggt gccggcgctg ctcctctgcc cggtctgcag cgcagccgta
     1801 tccaggtggt gttccgaacg agccatctgc gtctccatgg aaggcgccct cgccatcgtt
     1861 gggccagatc tgcccggtct tgcggacgcc cacgatggtg gcctcggaca cgacaaccgg
     1921 gcagtgccgg tgccgcttat ggcactgcgg cgtgggggga ctgctgctgt cgaaggactg
     1981 ttgcgagttc tgcgagggag agcggctttt gtcccggaac atgcggtagg tggtggggct
     2041 tggggacacg cggctggact ggccagagga gaagtcctcc tcgctggagg tgaggttctc
     2101 attggagctg cagtccgggg tatagccgcc tccgcaatcc tcaaaactcc ggggggagta
     2161 ggacctgcgg ggccaggtaa ggcgcttctc ctgctcagag gtgctctggc tgcgcaggag
     2221 cgggcccttg ccctcccctt ccatcatgcc cccgacgtag atgctctggt agggctggta
     2281 ctccaggggc ggccaagggg gcctgctacc gccattggcg tcgatcaggt tgtccttcag
     2341 gaagcggggg ttcaactcgg cgtcctcgta gtcgccgtcg acgccgcagc tgctctccga
     2401 ggagcgtccc cggtaagggg gcctggatgc atcccccacg ctcgagcccg cgccgtgctg
     2461 ggactttttg cgctccatct gcatggcctg gctgcccagg gagctgatgc ggtccgacac
     2521 ctctttgtcg ttgaccttca ccaggccgcg ctcgtggtga aactcgacgt tcacgtagaa
     2581 gggcttctcg gcgtccgccc cgggctggcc atggcccttg cgtatccgct cgaagttgga
     2641 cctgagcgcc gccacgctgg cggggggtcc ccggtcgtcc cgttcccccg acgcggctgc
     2701 cccgggcctg cgggcggtcc cgggcctggc cttacccgga gaaccctcgc cgtcgggccg
     2761 ggcctcgggc tcctcggcgg gcggcgggtc ggctccgtcg gcgggcgctg gctgcgggcg
     2821 cgacgcggac gctcggggct cggaggcgcc gtcgggggcc tgcgccgcgc gccggaagcc
     2881 ccatcgctgc cggtcatagc tcttcttttc cttggccagc aacgtctgca ggtagatcat
     2941 gcggaagcgc tcctggttca cctcctgctc caggcgccga atggaggcct tgcagcgctc
     3001 cagctcctgc tcgatgtcgc ccactgagcg cagctccatg cgcgggggct ctgagtccgg
     3061 gaactgcgcc ttccacgcct ccgcgaagcc caccgggtcc accagatatc tgcagaattc
     3121 tttatcgtca tcgtctttgt aatccatgtt ttgattcccg ggggatccgg taccaagctt
     3181 gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag
     3241 ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     3301 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     3361 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     3421 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     3481 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     3541 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     3601 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     3661 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     3721 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     3781 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacgcg
     3841 cgcgtaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag
     3901 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac
     3961 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga
     4021 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc
     4081 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg
     4141 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa
     4201 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac
     4261 caccacaccc gccgcgctta atgcgccgct acagggcgcg tcaggtggca cttttcgggg
     4321 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
     4381 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
     4441 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
     4501 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
     4561 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
     4621 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga
     4681 cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta
     4741 ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc
     4801 tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc
     4861 gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg
     4921 ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc
     4981 aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca
     5041 acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct
     5101 tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat
     5161 cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg
     5221 gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat
     5281 taagcattgg taactgtcag accaagttta ctcatatata ctttagattg atttattgaa
     5341 aaaggaagag tcctgaggcg gaaagaacca gctgtggaat gtgtgtcagt tagggtgtgg
     5401 aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc
     5461 aaccaggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
     5521 caattagtca gcaaccatag tcccgcccct aactccgccc atcccgcccc taactccgcc
     5581 cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg cagaggccga
     5641 ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg gaggcctagg
     5701 cttttgcaaa gatcgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
     5761 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
     5821 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
     5881 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaagac gaggcagcgc
     5941 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
     6001 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
     6061 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
     6121 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
     6181 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
     6241 cgccagccga actgttcgcc aggctcaagg cgagcatgcc cgacggcgag gatctcgtcg
     6301 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
     6361 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
     6421 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
     6481 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
     6541 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
     6601 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
     6661 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ctagggggag
     6721 gctaactgaa acacggaagg agacaatacc ggaaggaacc cgcgctatga cggcaataaa
     6781 aagacagaat aaaacgcacg gtgttgggtc gtttgttcat aaacgcgggg ttcggtccca
     6841 gggctggcac tctgtcgata ccccaccgag accccattgg ggccaatacg cccgcgtttc
     6901 ttccttttcc ccaccccacc ccccaagttc gggtgaaggc ccagggctcg cagccaacgt
     6961 cggggcggca ggccctgcca tagcctcagg ttactcatat atactttaga ttgatttaaa
     7021 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     7081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     7141 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     7201 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     7261 tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca
     7321 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     7381 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     7441 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     7501 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     7561 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     7621 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     7681 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     7741 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     7801 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gccactggtt ctttccgcct
     7861 cagaagccat agagcccacc gcatccccag catgcctgct attgtcttcc caatcctccc
     7921 ccttgctgtc ctgccccacc ccacccccca gaatagaatg acacctactc agacaatgcg
     7981 atgcaatttc ctcattttat taggaaagga cagtgggagt ggcaccttcc agggtcaagg
     8041 aaggcacggg ggaggggcaa acaacagatg gctggcaact agaaggcaca gtcgaggctg
     8101 atcagcgagc tctagcattt aggtgacact atagaatagg gccctctaga ctagtcatct
     8161 actcgagcgg ccgcctacct ctgcactatg tcactgattt ccttcaccga actgaggagc
     8221 ttgctgaagt cctgagtggc cgctggacca ctgcctgctg tcgccgggca gatctgaagc
     8281 tcccggagat tattctccag tttgttgatg gcctctcgga aggcaaactt gttcctcatt
     8341 tgctggatgg aatccacata gctcacgcag aacgtgtaga ggtttttgcc ggcctccagc
     8401 actgcgctgt ggctggccat ctgctcggag ttcctagaga tggcgaggca cagcgcctcg
     8461 gtgctgtcca ggaccacgcc cttggtgatg gcgccgctgg cgatccgctc tggaggctgg
     8521 cgggttttcc gaagagacac tcgggttgat atgagaggga tgaaggcggt ggaagacggc
     8581 tggtcccctg ccagggccga ggatgctgat ggcaacgtgg agggaacggg ggctgggctg
     8641 atgggggtcc ccgacggctt ggcggactgt ggctttggag tggccgggag cacgggcttt
     8701 ttgaggccct ctcccggctg gctgggcttg gcagcgtcac tgttcacagc atcaaccaga
     8761 ctcgtggctt ttgtctttgc gcccaggact gcctccccgg ccgcctcctg gctcgggctc
     8821 tgcgagggct ttcctccagc cttccctgca gaggctgctg gtgggggcgg cggggcaggt
     8881 ttgagcctgg acaatttccc cttgtccctc cctggcgact cagaggagtg cttgtgcctc
     8941 ctcactctgg actcctcggc ggggcccttg ctggtgcccc ctggtgcacc tgagcctgct
     9001 ttgctggtgg gggtcactgg ctcagctgca gcaggggtcc ctaaggcact gccctttcca
     9061 gcttcttcct tgtgggggag gcccgaggca ggggccacgg tgacctgccg ccggaggggt
     9121 tttggagtca ggttgggcgg gctggagccc gggctggact ccatgatgtc tttgaagacc
     9181 tcatcagcag cttcctcatt ctttttcacc agcctggggg gaggcgttac tgtgcctcgg
     9241 gtcacctggt cagacctgtt gatcaaggcg ctgaacaagt tggtcttttt gtctttgggg
     9301 agaaggcgct catcttcatt caggccgccc tccgggggac ctcgctcttt tcgagggagc
     9361 aatggagaca cggcaggctc atggtccaga ggatcgctct ctccctggcc cttggagtga
     9421 ggcatctcag gcacgtcagt ggtgtctctg tgctctgcag ctctcctgga ggtcctcgtc
     9481 ttggtgggca gctctggggc ctgcagcaag gtactcacag ccccacggac gccttgtttc
     9541 cccagctcct tttccacttc gtctgagata ctggattcct ggaacattgt ttcaaaggct
     9601 tggtggattt cagcaaagga gggccggtca gagggattcc actgccaaca tgctcgcatg
     9661 agttcataga ccttctctgg gcagccttct gggcgctcca tgcggtagtc cttctctagc
     9721 agctcataca cctgggacag gtcaattccc gggtaagggg acatgccata ggtagcaatt
     9781 tcccaaagca atactccaaa tgcccagacg tcggacttga tggagaactt gttgtaggcc
     9841 aggctctcgg gtgcagtcca tttgatgggg aacttggctc cagcatgggc tgtgtaggtg
     9901 tcccctgtca tcaacctgct caggccaaaa tcagctacct tcaccaagtg gttctcccct
     9961 accaggcagt ttcgggcagc aagatctctg tggatgaagt ttttcttctc caggtactcc
    10021 atggctgacg agatctgagt ggccatgtac agcagcacca cggcgttcac ctcctgccgg
    10081 ttgcactccc tcaggtagtc caggaggttc ccgtaggtca tgaactcagt gatgatatag
    10141 aacgggggct cccgggtgca gaccccaagg agctgcacca ggttagggtg tttgatctct
    10201 ttcatgactg cagcttcttt caagaactct tccacctcca tggtgtcctc cttcaaggtc
    10261 ttcacggcca ccgtcaggct gtatttcttc cacacgccct cgtacacctc cccgtactgg
    10321 cccccgccca gcttgtgctt catggtgatg tccgtgcgtt ccatctccca cttgtcgtag
    10381 ttgggggaca caccatagac agtgggcttg ttgcgctttg gggctggata atggagcgtg
    10441 gtgatgagcc cgtcggccac cgttgaatga tgatgaacca actcggccag ggtgttgaag
    10501 cggctctcgg aggagacgta gagcttgcca tcagaagcag tgttgatcct gtaatggtac
    10561 accctccctt cgtatctcag cgagatggac ctctggccag gactgctctc actctcacgc
    10621 accaagaagc tgccattgat cccgctgctc agcagatact cagcggcatt
//
