LOCUS       sequence-407821-        9674 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BCR-ABL oncogene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9674)
  AUTHORS   Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky 
            NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek 
            L, Krejci P
  TITLE     Elucidation of protein interactions necessary for the maintenance of
            the BCR-ABL signaling complex.
  JOURNAL   Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. doi: 
            10.1007/s00018-019-03397-7. Epub 2019 Dec 9.
  PUBMED    31820037
REFERENCE   2  (bases 1 to 9674)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-019-03397-7"; journalName: "Cell Mol Life Sci"; 
            date: "2020-10"; volume: "77"; issue: "19"; pages: "3885-3903"
COMMENT     Sequence Label: pcDNA3.1-BCR-ABL-p210-Delta-ST-FLAG
FEATURES             Location/Qualifiers
     source          1..9674
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(3693..3711)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3694..3711)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3731..3748)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3737..3961
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4093..4681)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4173..4192)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5010..5057
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5082..5101
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5289..6083)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5289..6083)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5400..5419)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6010..6029
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6118..6475)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6132..6267
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6284..6475
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6432..6452
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(6535..7326,7327..7395))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7158..7177
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7396..7500)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7527..7982
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7614..7633)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7824..7845
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        7994..8373
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8374..8577
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8528..8548
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8622..8641
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8622..8640
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8684..8707
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cccggagttt tgaggattgc ggaggcggct ataccccgga ctgcagctcc aatgagaacc
       61 tcacctccag cgaggaggac ttctcctctg gccagtccag ccgcgtgtcc ccaagcccca
      121 ccacctaccg catgttccgg gacaaaagcc gctctccctc gcagaactcg caacagtcct
      181 tcgacagcag cagtcccccc acgccgcagt gccataagcg gcaccggcac tgcccggttg
      241 tcgtgtccga ggccaccatc gtgggcgtcc gcaagaccgg gcagatctgg cccaacgatg
      301 gcgagggcgc cttccatgga gacgcagatg gctcgttcgg aacaccacct ggatacggct
      361 gcgctgcaga ccgggcagag gagcagcgcc ggcaccaaga tgggctgccc tacattgatg
      421 actcgccctc ctcatcgccc cacctcagca gcaagggcag gggcagccgg gatgcgctgg
      481 tctcgggagc cctggagtcc actaaagcga gtgagctgga cttggaaaag ggcttggaga
      541 tgagaaaatg ggtcctgtcg ggaatcctgg ctagcgagga gacttacctg agccacctgg
      601 aggcactgct gctgcccatg aagcctttga aagccgctgc caccacctct cagccggtgc
      661 tgacgagtca gcagatcgag accatcttct tcaaagtgcc tgagctctac gagatccaca
      721 aggagttcta tgatgggctc ttcccccgcg tgcagcagtg gagccaccag cagcgggtgg
      781 gcgacctctt ccagaagctg gccagccagc tgggtgtgta ccgggccttc gtggacaact
      841 acggagttgc catggaaatg gctgagaagt gctgtcaggc caatgctcag tttgcagaaa
      901 tctccgagaa cctgagagcc agaagcaaca aagatgccaa ggatccaacg accaagaact
      961 ctctggaaac tctgctctac aagcctgtgg accgtgtgac gaggagcacg ctggtcctcc
     1021 atgacttgct gaagcacact cctgccagcc accctgacca ccccttgctg caggacgccc
     1081 tccgcatctc acagaacttc ctgtccagca tcaatgagga gatcacaccc cgacggcagt
     1141 ccatgacggt gaagaaggga gagcaccggc agctgctgaa ggacagcttc atggtggagc
     1201 tggtggaggg ggcccgcaag ctgcgccacg tcttcctgtt caccgacctg cttctctgca
     1261 ccaagctcaa gaagcagagc ggaggcaaaa cgcagcagta tgactgcaaa tggtacattc
     1321 cgctcacgga tctcagcttc cagatggtgg atgaactgga ggcagtgccc aacatccccc
     1381 tggtgcccga tgaggagctg gacgctttga agatcaagat ctcccagatc aagagtgaca
     1441 tccagagaga gaagagggcg aacaagggca gcaaggctac ggagaggctg aagaagaagc
     1501 tgtcggagca ggagtcactg ctgctgctta tgtctcccag catggccttc agggtgcaca
     1561 gccgcaacgg caagagttac acgttcctga tctcctctga ctatgagcgt gcagagtgga
     1621 gggagaacat ccgggagcag cagaagaagt gtttcagaag cttctccctg acatccgtgg
     1681 agctgcagat gctgaccaac tcgtgtgtga aactccagac tgtccacagc attccgctga
     1741 ccatcaataa ggaagatgat gagtctccgg ggctctatgg gtttctgaat gtcatcgtcc
     1801 actcagccac tggatttaag cagagttcaa aagcccttca gcggccagta gcatctgact
     1861 ttgagcctca gggtctggag cacagagaca ccactgacgt gcctgagatg cctcactcca
     1921 agggccaggg agagagcgat cctctggacc atgagcctgc cgtgtctcca ttgctccctc
     1981 gaaaagagcg aggtcccccg gagggcggcc tgaatgaaga tgagcgcctt ctccccaaag
     2041 acaaaaagac caacttgttc agcgccttga tcaagaagaa gaagaagaca gccccaaccc
     2101 ctcccaaacg cagcagctcc ttccgggaga tggacggcca gccggagcgc agaggggccg
     2161 gcgaggaaga gggccgagac atcagcaacg gggcactggc tttcaccccc ttggacacag
     2221 ctgacccagc caagtcccca aagcccagca atggggctgg ggtccccaat ggagccctcc
     2281 gggagtctgg gggctcaggc ttccggtctc cccacctgtg gaagaagtcc agcacgctga
     2341 ccagcagccg cctagccacc ggcgaggagg agggcggtgg cagctccagc aagcgcttcc
     2401 tgcgctcttg ctccgcctcc tgcgttcccc atggggccaa ggacacggag tggaggtcag
     2461 tcacgctgcc tcgggacttg cagtccacgg gaagacagtt tgactcgtcc acatttggag
     2521 ggcacaaaag tgagaagccg gctctgcctc ggaagagggc aggggagaac aggtctgacc
     2581 aggtgacccg aggcacagta acgcctcccc ccaggctggt gaaaaagaat gaggaagctg
     2641 ctgatgaggt cttcaaagac atcatggagt ccagcccggg ctccagcccg cccaacctga
     2701 ctccaaaacc cctccggcgg caggtcaccg tggcccctgc ctcgggcctc ccccacaagg
     2761 aagaagctgg aaagggcagt gccttaggga cccctgctgc agctgagcca gtgaccccca
     2821 ccagcaaagc aggctcaggt gcaccagggg gcaccagcaa gggccccgcc gaggagtcca
     2881 gagtgaggag gcacaagcac tcctctgagt cgccagggag ggacaagggg aaattgtcca
     2941 ggctcaaacc tgccccgccg cccccaccag cagcctctgc agggaaggct ggaggaaagc
     3001 cctcgcagag cccgagccag gaggcggccg gggaggcagt cctgggcgca aagacaaaag
     3061 ccacgagtct ggttgatgct gtgaacagtg acgctgccaa gcccagccag ccgggagagg
     3121 gcctcaaaaa gcccgtgctc ccggccactc caaagccaca gtccgccaag ccgtcgggga
     3181 cccccatcag cccagccccc gttccctcca cgttgccatc agcatcctcg gccctggcag
     3241 gggaccagcc gtcttccacc gccttcatcc ctctcatatc aacccgagtg tctcttcgga
     3301 aaacccgcca gcctccagag cggatcgcca gcggcgccat caccaagggc gtggtcctgg
     3361 acagcaccga ggcgctgtgc ctcgccatct ctaggaactc cgagcagatg gccagccaca
     3421 gcgcagtgct ggaggccggc aaaaacctct acacgttctg cgtgagctat gtggattcca
     3481 tccagcaaat gaggaacaag tttgccttcc gagaggccat caacaaactg gagaataatc
     3541 tccgggagct tcagatctgc ccggcgacag caggcagtgg tccagcggcc actcaggact
     3601 tcagcaagct cctcagttcg gtgaaggaaa tcagtgacat agtgcagagg taggcggccg
     3661 ctcgagtaga tgactagtct agagggccct attctatagt gtcacctaaa tgctagagct
     3721 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
     3781 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
     3841 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
     3901 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
     3961 gcttctgagg cggaaagaac cagtggcggt aatacggtta tccacagaat caggggataa
     4021 cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc
     4081 gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc
     4141 aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag
     4201 ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct
     4261 cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta
     4321 ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc
     4381 cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc
     4441 agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt
     4501 gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct
     4561 gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     4621 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     4681 agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta
     4741 agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa
     4801 atgaagtttt aaatcaatct aaagtatata tgagtaacct gaggctatgg cagggcctgc
     4861 cgccccgacg ttggctgcga gccctgggcc ttcacccgaa cttggggggt ggggtgggga
     4921 aaaggaagaa acgcgggcgt attggcccca atggggtctc ggtggggtat cgacagagtg
     4981 ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac ccaacaccgt gcgttttatt
     5041 ctgtcttttt attgccgtca tagcgcgggt tccttccggt attgtctcct tccgtgtttc
     5101 agttagcctc cccctagggt gggcgaagaa ctccagcatg agatccccgc gctggaggat
     5161 catccagccg gcgtcccgga aaacgattcc gaagcccaac ctttcataga aggcggcggt
     5221 ggaatcgaaa tctcgtgatg gcaggttggg cgtcgcttgg tcggtcattt cgaaccccag
     5281 agtcccgctc agaagaactc gtcaagaagg cgatagaagg cgatgcgctg cgaatcggga
     5341 gcggcgatac cgtaaagcac gaggaagcgg tcagcccatt cgccgccaag ctcttcagca
     5401 atatcacggg tagccaacgc tatgtcctga tagcggtccg ccacacccag ccggccacag
     5461 tcgatgaatc cagaaaagcg gccattttcc accatgatat tcggcaagca ggcatcgcca
     5521 tgggtcacga cgagatcctc gccgtcgggc atgctcgcct tgagcctggc gaacagttcg
     5581 gctggcgcga gcccctgatg ctcttcgtcc agatcatcct gatcgacaag accggcttcc
     5641 atccgagtac gtgctcgctc gatgcgatgt ttcgcttggt ggtcgaatgg gcaggtagcc
     5701 ggatcaagcg tatgcagccg ccgcattgca tcagccatga tggatacttt ctcggcagga
     5761 gcaaggtgag atgacaggag atcctgcccc ggcacttcgc ccaatagcag ccagtccctt
     5821 cccgcttcag tgacaacgtc gagcacagct gcgcaaggaa cgcccgtcgt ggccagccac
     5881 gatagccgcg ctgcctcgtc ttgcagttca ttcagggcac cggacaggtc ggtcttgaca
     5941 aaaagaaccg ggcgcccctg cgctgacagc cggaacacgg cggcatcaga gcagccgatt
     6001 gtctgttgtg cccagtcata gccgaatagc ctctccaccc aagcggccgg agaacctgcg
     6061 tgcaatccat cttgttcaat catgcgaaac gatcctcatc ctgtctcttg atcgatcttt
     6121 gcaaaagcct aggcctccaa aaaagcctcc tcactacttc tggaatagct cagaggccga
     6181 ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg gagaatgggc
     6241 ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggacta tggttgctga
     6301 ctaattgaga tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca
     6361 cctggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     6421 ggactttcca caccctaact gacacacatt ccacagctgg ttctttccgc ctcaggactc
     6481 ttcctttttc aataaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca
     6541 atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc
     6601 ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc
     6661 tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc
     6721 agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat
     6781 taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt
     6841 tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc
     6901 cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag
     6961 ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt
     7021 tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac
     7081 tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg
     7141 cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat
     7201 tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc
     7261 gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc
     7321 tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa
     7381 atgttgaata ctcatactct tcctttttca atattattga agcatttatc agggttattg
     7441 tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg
     7501 cacatttccc cgaaaagtgc cacctgacgc gccctgtagc ggcgcattaa gcgcggcggg
     7561 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     7621 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     7681 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7741 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7801 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7861 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7921 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7981 ttacgcgcgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     8041 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     8101 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     8161 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     8221 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     8281 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     8341 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     8401 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     8461 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     8521 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     8581 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
     8641 gagacccaag cttggtaccg gatcccccgg gaatcaaaac atggattaca aagacgatga
     8701 cgataaagaa ttctgcagat atctggtgga cccggtgggc ttcgcggagg cgtggaaggc
     8761 gcagttcccg gactcagagc ccccgcgcat ggagctgcgc tcagtgggcg acatcgagca
     8821 ggagctggag cgctgcaagg cctccattcg gcgcctggag caggaggtga accaggagcg
     8881 cttccgcatg atctacctgc agacgttgct ggccaaggaa aagaagagct atgaccggca
     8941 gcgatggggc ttccggcgcg cggcgcaggc ccccgacggc gcctccgagc cccgagcgtc
     9001 cgcgtcgcgc ccgcagccag cgcccgccga cggagccgac ccgccgcccg ccgaggagcc
     9061 cgaggcccgg cccgacggcg agggttctcc gggtaaggcc aggcccggga ccgcccgcag
     9121 gcccggggca gccgcgtcgg gggaacggga cgaccgggga ccccccgcca gcgtggcggc
     9181 gctcaggtcc aacttcgagc ggatacgcaa gggccatggc cagcccgggg cggacgccga
     9241 gaagcccttc tacgtgaacg tcgagtttca ccacgagcgc ggcctggtga aggtcaacga
     9301 caaagaggtg tcggaccgca tcagctccct gggcagccag gccatgcaga tggagcgcaa
     9361 aaagtcccag cacggcgcgg gctcgagcgt gggggatgca tccaggcccc cttaccgggg
     9421 acgctcctcg gagagcagct gcggcgtcga cggcgactac gaggacgccg agttgaaccc
     9481 ccgcttcctg aaggacaacc tgatcgacgc caatggcggt agcaggcccc cttggccgcc
     9541 cctggagtac cagccctacc agagcatcta cgtcgggggc atgatggaag gggagggcaa
     9601 gggcccgctc ctgcgcagcc agagcacctc tgagcaggag aagcgcctta cctggccccg
     9661 caggtcctac tccc
//
