LOCUS       sequence-407822-        9698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of BCR-ABL oncogene in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9698)
  AUTHORS   Gregor T, Bosakova MK, Nita A, Abraham SP, Fafilek B, Cernohorsky 
            NH, Rynes J, Foldynova-Trantirkova S, Zackova D, Mayer J, Trantirek 
            L, Krejci P
  TITLE     Elucidation of protein interactions necessary for the maintenance of
            the BCR-ABL signaling complex.
  JOURNAL   Cell Mol Life Sci. 2020 Oct;77(19):3885-3903. doi: 
            10.1007/s00018-019-03397-7. Epub 2019 Dec 9.
  PUBMED    31820037
REFERENCE   2  (bases 1 to 9698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1007/s00018-019-03397-7"; journalName: "Cell Mol Life Sci"; 
            date: "2020-10"; volume: "77"; issue: "19"; pages: "3885-3903"
COMMENT     Sequence Label: pcDNA3.1-BCR-ABL-p210-BT-FLAG
FEATURES             Location/Qualifiers
     source          1..9698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(3705..3723)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3706..3723)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3743..3760)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3749..3973
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      complement(4105..4693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4185..4204)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     polyA_signal    5022..5069
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     5094..5113
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(5301..6095)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     CDS             complement(5301..6095)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5412..5431)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     6022..6041
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(6130..6487)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6144..6279
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        6296..6487
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     6444..6464
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             complement(join(6547..7338,7339..7407))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7170..7189
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7408..7512)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      7539..7994
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7626..7645)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7836..7857
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     enhancer        8006..8385
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        8386..8589
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     8540..8560
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     8634..8653
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        8634..8652
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             8696..8719
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 cggagaggct gaagaagaag ctgtcggagc aggagtcact gctgctgctt atgtctccca
       61 gcatggcctt cagggtgcac agccgcaacg gcaagagtta cacgttcctg atctcctctg
      121 actatgagcg tgcagagtgg agggagaaca tccgggagca gcagaagaag tgtttcagaa
      181 gcttctccct gacatccgtg gagctgcaga tgctgaccaa ctcgtgtgtg aaactccaga
      241 ctgtccacag cattccgctg accatcaata aggaagatga tgagtctccg gggctctatg
      301 ggtttctgaa tgtcatcgtc cactcagcca ctggatttaa gcagagttca aaagcccttc
      361 agcggccagt agcatctgac tttgagcctc agggtctgag tgaagccgct cgttggaact
      421 ccaaggaaaa ccttctcgct ggacccagtg aaaatgaccc caaccttttc gttgcactgt
      481 atgattttgt ggccagtgga gataacactc taagcataac taaaggtgaa aagctccggg
      541 tcttaggcta taatcacaat ggggaatggt gtgaagccca aaccaaaaat ggccaaggct
      601 gggtcccaag caactacatc acgccagtca acagtctgga gaaacactcc tggtaccatg
      661 ggcctgtgtc ccgcaatgcc gctgagtatc tgctgagcag cgggatcaat ggcagcttct
      721 tggtgcgtga gagtgagagc agtcctggcc agaggtccat ctcgctgaga tacgaaggga
      781 gggtgtacca ttacaggatc aacactgctt ctgatggcaa gctctacgtc tcctccgaga
      841 gccgcttcaa caccctggcc gagttggttc atcatcattc aacggtggcc gacgggctca
      901 tcaccacgct ccattatcca gccccaaagc gcaacaagcc cactgtctat ggtgtgtccc
      961 ccaactacga caagtgggag atggaacgca cggacatcac catgaagcac aagctgggcg
     1021 ggggccagta cggggaggtg tacgagggcg tgtggaagaa atacagcctg acggtggccg
     1081 tgaagacctt gaaggaggac accatggagg tggaagagtt cttgaaagaa gctgcagtca
     1141 tgaaagagat caaacaccct aacctggtgc agctccttgg ggtctgcacc cgggagcccc
     1201 cgttctatat catcactgag ttcatgacct acgggaacct cctggactac ctgagggagt
     1261 gcaaccggca ggaggtgaac gccgtggtgc tgctgtacat ggccactcag atctcgtcag
     1321 ccatggagta cctggagaag aaaaacttca tccacagaga tcttgctgcc cgaaactgcc
     1381 tggtagggga gaaccacttg gtgaaggtag ctgattttgg cctgagcagg ttgatgacag
     1441 gggacaccta cacagcccat gctggagcca agttccccat caaatggact gcacccgaga
     1501 gcctggccta caacaagttc tccatcaagt ccgacgtctg ggcatttgga gtattgcttt
     1561 gggaaattgc tacctatggc atgtcccctt acccgggaat tgacctgtcc caggtgtatg
     1621 agctgctaga gaaggactac cgcatggagc gcccagaagg ctgcccagag aaggtctatg
     1681 aactcatgcg agcatgttgg cagtggaatc cctctgaccg gccctccttt gctgaaatcc
     1741 accaagcctt tgaaacaatg ttccaggaat ccagtatctc agacgaagtg gaaaaggagc
     1801 tggggaaaca aggcgtccgt ggggctgtga gtaccttgct gcaggcccca gagctgccca
     1861 ccaagacgag gacctccagg agagctgcag agcacagaga caccactgac gtgcctgaga
     1921 tgcctcactc caagggccag ggagagagcg atcctctgga ccatgagcct gccgtgtctc
     1981 cattgctccc tcgaaaagag cgaggtcccc cggagggcgg cctgaatgaa gatgagcgcc
     2041 ttctccccaa agacaaaaag accaacttgt tcagcgcctt gatcaagaag aagaagaaga
     2101 cagccccaac ccctcccaaa cgcagcagct ccttccggga gatggacggc cagccggagc
     2161 gcagaggggc cggcgaggaa gagggccgag acatcagcaa cggggcactg gctttcaccc
     2221 ccttggacac agctgaccca gccaagtccc caaagcccag caatggggct ggggtcccca
     2281 atggagccct ccgggagtct gggggctcag gcttccggtc tccccacctg tggaagaagt
     2341 ccagcacgct gaccagcagc cgcctagcca ccggcgagga ggagggcggt ggcagctcca
     2401 gcaagcgctt cctgcgctct tgctccgcct cctgcgttcc ccatggggcc aaggacacgg
     2461 agtggaggtc agtcacgctg cctcgggact tgcagtccac gggaagacag tttgactcgt
     2521 ccacatttgg agggcacaaa agtgagaagc cggctctgcc tcggaagagg gcaggggaga
     2581 acaggtctga ccaggtgacc cgaggcacag taacgcctcc ccccaggctg gtgaaaaaga
     2641 atgaggaagc tgctgatgag gtcttcaaag acatcatgga gtccagcccg ggctccagcc
     2701 cgcccaacct gactccaaaa cccctccggc ggcaggtcac cgtggcccct gcctcgggcc
     2761 tcccccacaa ggaagaagct ggaaagggca gtgccttagg gacccctgct gcagctgagc
     2821 cagtgacccc caccagcaaa gcaggctcag gtgcaccagg gggcaccagc aagggccccg
     2881 ccgaggagtc cagagtgagg aggcacaagc actcctctga gtcgccaggg agggacaagg
     2941 ggaaattgtc caggctcaaa cctgccccgc cgcccccacc agcagcctct gcagggaagg
     3001 ctggaggaaa gccctcgcag agcccgagcc aggaggcggc cggggaggca gtcctgggcg
     3061 caaagacaaa agccacgagt ctggttgatg ctgtgaacag tgacgctgcc aagcccagcc
     3121 agccgggaga gggcctcaaa aagcccgtgc tcccggccac tccaaagcca cagtccgcca
     3181 agccgtcggg gacccccatc agcccagccc ccgttccctc cacgttgcca tcagcatcct
     3241 cggccctggc aggggaccag ccgtcttcca ccgccttcat ccctctcata tcaacccgag
     3301 tgtctcttcg gaaaacccgc cagcctccag agcggatcgc cagcggcgcc atcaccaagg
     3361 gcgtggtcct ggacagcacc gaggcgctgt gcctcgccat ctctaggaac tccgagcaga
     3421 tggccagcca cagcgcagtg ctggaggccg gcaaaaacct ctacacgttc tgcgtgagct
     3481 atgtggattc catccagcaa atgaggaaca agtttgcctt ccgagaggcc atcaacaaac
     3541 tggagaataa tctccgggag cttcagatct gcccggcgac agcaggcagt ggtccagcgg
     3601 ccactcagga cttcagcaag ctcctcagtt cggtgaagga aatcagtgac atagtgcaga
     3661 ggtaggcggc cgctcgagta gatgactagt ctagagggcc ctattctata gtgtcaccta
     3721 aatgctagag ctcgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     3781 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     3841 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     3901 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     3961 gtgggctcta tggcttctga ggcggaaaga accagtggcg gtaatacggt tatccacaga
     4021 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     4081 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     4141 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     4201 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     4261 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     4321 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     4381 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     4441 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     4501 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
     4561 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     4621 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     4681 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     4741 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     4801 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaac ctgaggctat
     4861 ggcagggcct gccgccccga cgttggctgc gagccctggg ccttcacccg aacttggggg
     4921 gtggggtggg gaaaaggaag aaacgcgggc gtattggccc caatggggtc tcggtggggt
     4981 atcgacagag tgccagccct gggaccgaac cccgcgttta tgaacaaacg acccaacacc
     5041 gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg gttccttccg gtattgtctc
     5101 cttccgtgtt tcagttagcc tccccctagg gtgggcgaag aactccagca tgagatcccc
     5161 gcgctggagg atcatccagc cggcgtcccg gaaaacgatt ccgaagccca acctttcata
     5221 gaaggcggcg gtggaatcga aatctcgtga tggcaggttg ggcgtcgctt ggtcggtcat
     5281 ttcgaacccc agagtcccgc tcagaagaac tcgtcaagaa ggcgatagaa ggcgatgcgc
     5341 tgcgaatcgg gagcggcgat accgtaaagc acgaggaagc ggtcagccca ttcgccgcca
     5401 agctcttcag caatatcacg ggtagccaac gctatgtcct gatagcggtc cgccacaccc
     5461 agccggccac agtcgatgaa tccagaaaag cggccatttt ccaccatgat attcggcaag
     5521 caggcatcgc catgggtcac gacgagatcc tcgccgtcgg gcatgctcgc cttgagcctg
     5581 gcgaacagtt cggctggcgc gagcccctga tgctcttcgt ccagatcatc ctgatcgaca
     5641 agaccggctt ccatccgagt acgtgctcgc tcgatgcgat gtttcgcttg gtggtcgaat
     5701 gggcaggtag ccggatcaag cgtatgcagc cgccgcattg catcagccat gatggatact
     5761 ttctcggcag gagcaaggtg agatgacagg agatcctgcc ccggcacttc gcccaatagc
     5821 agccagtccc ttcccgcttc agtgacaacg tcgagcacag ctgcgcaagg aacgcccgtc
     5881 gtggccagcc acgatagccg cgctgcctcg tcttgcagtt cattcagggc accggacagg
     5941 tcggtcttga caaaaagaac cgggcgcccc tgcgctgaca gccggaacac ggcggcatca
     6001 gagcagccga ttgtctgttg tgcccagtca tagccgaata gcctctccac ccaagcggcc
     6061 ggagaacctg cgtgcaatcc atcttgttca atcatgcgaa acgatcctca tcctgtctct
     6121 tgatcgatct ttgcaaaagc ctaggcctcc aaaaaagcct cctcactact tctggaatag
     6181 ctcagaggcc gaggcggcct cggcctctgc ataaataaaa aaaattagtc agccatgggg
     6241 cggagaatgg gcggaactgg gcggagttag gggcgggatg ggcggagtta ggggcgggac
     6301 tatggttgct gactaattga gatgcatgct ttgcatactt ctgcctgctg gggagcctgg
     6361 ggactttcca cacctggttg ctgactaatt gagatgcatg ctttgcatac ttctgcctgc
     6421 tggggagcct ggggactttc cacaccctaa ctgacacaca ttccacagct ggttctttcc
     6481 gcctcaggac tcttcctttt tcaataaatc aatctaaagt atatatgagt aaacttggtc
     6541 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     6601 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     6661 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     6721 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     6781 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     6841 gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     6901 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     6961 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     7021 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     7081 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     7141 gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     7201 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     7261 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     7321 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     7381 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     7441 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     7501 aggggttccg cgcacatttc cccgaaaagt gccacctgac gcgccctgta gcggcgcatt
     7561 aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc
     7621 gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca
     7681 agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc
     7741 caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt
     7801 tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac
     7861 aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc
     7921 ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt
     7981 aacgcttaca atttacgcgc gcgttgacat tgattattga ctagttatta atagtaatca
     8041 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
     8101 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
     8161 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
     8221 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
     8281 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
     8341 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
     8401 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
     8461 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
     8521 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
     8581 agcagagctc tctggctaac tagagaaccc actgcttact ggcttatcga aattaatacg
     8641 actcactata gggagaccca agcttggtac cggatccccc gggaatcaaa acatggatta
     8701 caaagacgat gacgataaag aattctgcag atatctggac ttggaaaagg gcttggagat
     8761 gagaaaatgg gtcctgtcgg gaatcctggc tagcgaggag acttacctga gccacctgga
     8821 ggcactgctg ctgcccatga agcctttgaa agccgctgcc accacctctc agccggtgct
     8881 gacgagtcag cagatcgaga ccatcttctt caaagtgcct gagctctacg agatccacaa
     8941 ggagttctat gatgggctct tcccccgcgt gcagcagtgg agccaccagc agcgggtggg
     9001 cgacctcttc cagaagctgg ccagccagct gggtgtgtac cgggccttcg tggacaacta
     9061 cggagttgcc atggaaatgg ctgagaagtg ctgtcaggcc aatgctcagt ttgcagaaat
     9121 ctccgagaac ctgagagcca gaagcaacaa agatgccaag gatccaacga ccaagaactc
     9181 tctggaaact ctgctctaca agcctgtgga ccgtgtgacg aggagcacgc tggtcctcca
     9241 tgacttgctg aagcacactc ctgccagcca ccctgaccac cccttgctgc aggacgccct
     9301 ccgcatctca cagaacttcc tgtccagcat caatgaggag atcacacccc gacggcagtc
     9361 catgacggtg aagaagggag agcaccggca gctgctgaag gacagcttca tggtggagct
     9421 ggtggagggg gcccgcaagc tgcgccacgt cttcctgttc accgacctgc ttctctgcac
     9481 caagctcaag aagcagagcg gaggcaaaac gcagcagtat gactgcaaat ggtacattcc
     9541 gctcacggat ctcagcttcc agatggtgga tgaactggag gcagtgccca acatccccct
     9601 ggtgcccgat gaggagctgg acgctttgaa gatcaagatc tcccagatca agagtgacat
     9661 ccagagagag aagagggcga acaagggcag caaggcta
//
