LOCUS       sequence-407835-       13989 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  NOT1 expression in Schneider cells for imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 13989)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 13989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: pAc5.1-NOT1 isoform C-mCherry-PH (EB5)
FEATURES             Location/Qualifiers
     source          1..13989
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             10116..10823
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(10261..10279)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     10529..10548
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             11295..11336
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             11346..11363
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(11386..11403)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11436..11570
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(11460..11479)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     11514..11533
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(12171..12759)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(12251..12270)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(12930..13721,13722..13790))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     13553..13572
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(13791..13895)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     13963..13981
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcgggtgca
     2581 tgaacgtaga gagccaactg aagacaccgc caacgcacat tcgaaatctg gtcaagcacg
     2641 tgaacaagcg aaattttaat gagagcagcg agcaaataaa tcagttcgtt aaagagcacg
     2701 gcctggaagc ggatcgcagc agcctgcgcc acctgttctc cgtgatcaac ttcagcgatc
     2761 tcgtgccatc agttacggtc cagctgcagg cgaagctcct gggcatccag ttggagcgtc
     2821 agcttcacag ttcatcgttt gtgtccaaca tctgctacgc ttttgaccag ttcttcgcca
     2881 gcaaccagaa gtccctgaag cctgttgcgg tagcggatct cgttggccag gtcgcccgac
     2941 tgaccggcat caacaaggtt tgtgagtgcg tctttgcctt ggccttgacc cactcctcgt
     3001 acacagaact caaacattct gccaggaaca acctaaaagg tagcctcagc gaacttatag
     3061 actcttactt gggtaataaa ggcaccggcc cagccgacag cggtctccgg gagatctcct
     3121 tcgatctact gcagtatttg ctgtgctgcc ttagcgagta cgtacaaccg caggtggaag
     3181 cacagttttt gatcaagctt cgtgaggagt ttccgcgcca ggcggtaccg ctggttctag
     3241 cgccgttcct ttacggctcg acgacggcga cgattgcggg agcaggtgcc agcgaaacgg
     3301 atgcggaggc cgaagcgacg actaatagca acagctccag tttcgaagca gatgctttga
     3361 acgaggtggg aatcgaggat atttatgacc atttaagcga gataatattc accaaccagg
     3421 gcaaaaataa tattatggac acatcttgga ttaatctaat ccttgaaatc ggttatgaat
     3481 ttacatcgag cgttgaagag tgtaagaatc atttgtgttc ccgcgagcga gaacgcgctg
     3541 agctccagtc caaagatgtc gccaagatcg tgggtcttat gtgccgacgg cactcctccc
     3601 tgctcgattg taatgtgaat ctaccgacgc cggcgaactt ctggcctggt cagggacagg
     3661 gcggtggcag caacagcagt ggctcttcgc agacacaaat cactccgcag caacagaacc
     3721 cgggcagcag taacaacaac gatggcagcg atggcaattc gtccagtgat aagaaggaca
     3781 agaaggagac gacggaggcc acgcaaactt ggaagccaga tgtctttgtg caggcgctca
     3841 aggaggtggt gcctcagctc aactggaagg acgtgtgcat ggaacttgat catcccgagt
     3901 tcgtgctgaa ggatcgcatc ggtttggagc ttttgctcac catccttcgg ctggccactg
     3961 gctctaacat atttccacat ccagaatgca tttaccgtca ctgggcaaac acggaaggtc
     4021 agctgtcgct tatcgcgaca atgctgaaga acccggatct cttttccttc gccgactttg
     4081 tatttagcca gccagcgttg gatgtgctta agacggctcc ggatgcggac aataaggaga
     4141 tctcgtcctg gaagtcgctt cacctggtgg aggtgctgct ctccattgcg gacaagggat
     4201 actatacgca ggtccatgag ctattcaagt tcccagcgca gaactgtccc gatgtgctat
     4261 ttttggcctt attgaacaca agtccgccta tgacgccact gcgtcaggat ctgtttaacc
     4321 aactgatacc aacgttcctt ggcaaccatc ccaactcgaa cgtgatcttg gccagcgcct
     4381 ggagctcgaa caacttccaa cttcgttcca atatcatgaa tgctatgtcc gagtggtatc
     4441 tccgaggcaa tgagttcgat caggtgaaac tgtcgcgcat cctggatctt gcccaggatt
     4501 taaaagcgct atcagcgctg ctcaacgccc gttcattttt attcattatc gacctggctt
     4561 gccttgcctc tcggcgcgag tacctgaagc tggaaaagtg gcttaccgat aaaattcgcg
     4621 aacatggaga gccttttatg caggctataa tcaaggttct gcaccgccgc tgcccgcagg
     4681 ttatcaacgc caaagtgcca gaggatcagc tgcctcccaa gcaggcgcag cttttgcccg
     4741 agacagttac aaccatgatc aactgtctgc aaacgtgcat caataactgc atgcagccgg
     4801 agatggtcga agtcatcatg caaatgaccg cgaacgtggc gattatggct aacaaggcgc
     4861 gtgcccaaca gcagcagcag ccaggattgg ttccgccgcc tccaccaaca attctgcgcg
     4921 gccatcgtgg tatggatctg cctggaggca tagtccctcc gccaccgcag caaccgttct
     4981 ccggaaacct caacgcgcag atgtttggac caggaatgga tccgctaacg aatatgtcca
     5041 acaacttagc cggcctcaac ctcagcggcc cgaacggagc atttaatttt ggaaacatgc
     5101 taacttctcc atcgcgtctg atgactccgg gagccaatcc ctatccgctg aacctcatgc
     5161 agatgccgca ggctccgcca ccacccaacg taggaaacct aggccgcatg ctgccagggg
     5221 gtccacaaca gcagactcca acgccgactc caacggcccc caatccaaac aaccctgtga
     5281 tggccgatct ccagatacct gtgtcaaagg aagttgaaga cgaggtcaac tcatactttc
     5341 agcgcatcta caaccaccag ccgaatccaa cgctgtccat cgatgaagtg ctggacattt
     5401 tgcaaagatt taaagagtca agtaaccggc gcgagcagga agtctttttg tgcatgctgc
     5461 gcaatctgtt cgaggaatat cgctttttct gtcaataccc agagaaagag ctacagatta
     5521 ctgcgcagct atttggtggc attattgatc gtaacctggt gcccactttt gttgcactgg
     5581 gcctttctct gcgctgtgtc ttggatgccc tccgcaagcc cgatggatct aagctttact
     5641 atttcggtgt gacggcactg gacagattca gaactcgatt gcacacctac aacaaatact
     5701 gcgagcatat ccgctccatt cctcacttct ctgacttccc gccgcacctt attcagtatg
     5761 tggaatacgg aatgcacggc caggagccgc cgccgcagaa gttgattgga ctcagcaata
     5821 cgattccttc ggcgatatct agcggaccag gaacggaacc aatttacagg aatagctcca
     5881 tgttaggtaa tatgcccgct gccaccccgg gatcgggacc gaaatcaaac gctgctgtat
     5941 cgcatgccac gagaatgaag tccatcgcca atgccaccaa tatcgacaca cttttggtgg
     6001 ccaaccagga agagaaagtg actgtgccac cagagcctgt gcaggacaaa actgccttta
     6061 ttttcaacaa cttgagccag ctgaacatac cgcagaagtg cgatgagata aaggagatta
     6121 tgaccaagga gtattggccg tggctagcgc aatatctggt cctcaaacgc gcatcaatgg
     6181 agttcaactt ccacacacta tattacaact ttttggatgc ccttaaaaac ggcgagatca
     6241 accgattcgt aaccaaagag accctacgca acatcaaggt actcttgcgc tccgataagg
     6301 gagttattaa tttctccgat cgaagtctgt tgaagaacct cggacactgg ttgggcatga
     6361 tgaccttagg tcgcaatcgc cccatcctgc agttggatct ggatcttaaa tcccttttgg
     6421 ctgaggctta ccacaagggc cagcaggaac tgctttttgt ggttcctttc gtagccaaga
     6481 tccttgagtc atccgccaag tctcgcatct tccgatcgcc caatccatgg acaatgggta
     6541 tcatgtacgt gcttgctgag cttcaccagg agccagacct taagctgaac ctgaagtttg
     6601 aaatcgaagt actctgcaaa acacttaatc tggagttggc caagttgcga caagtgatct
     6661 acctgaagga tcccaatcga actcaccgga ttgaagagca gatgtcgcaa ccaaaaccga
     6721 aacaacttga gccagtggct tctgcgccag ctctaccgcg tgaacagcaa tccccggcac
     6781 aacctccgcc accaccacaa cagcagcaac caccgcagca gcaggtacct ccaccgccat
     6841 cctctgcgga cgtggacgcc caaaatgcgg ccgctatgat gatggcagcg ggcggagcta
     6901 atagtactcc tggatcggta tcttcgccca atctacccac cgattccagc caggtggcac
     6961 taccgccacc agagccgcgc tattcatacg tggacgtaaa cgtgagcaac ttccagctta
     7021 ttggtcagca gttggtactg ccacccaata ctccgttcct gcacgccaat cccggaatca
     7081 aacacatggt cgtcaacgcc gtggagcgca cgataaccga ctggttgcag cctattgtgg
     7141 accgaagcat ccgcattgcc tgtgccacca ccgagcagat catccgcaag gacttcgccc
     7201 tggatgctga cgagaaccga atgcgcactg ccgctcatca gatggtacga aaccttgccg
     7261 ccggcatggc catgattacc ggtaaggatg agatagcgcg tgctattagc cagaatctgc
     7321 acaaggcctt gctgtcgggt ctaaatggga tgcccagcat ggctgagatc caagctgctg
     7381 ctatgcagtt agccagcgaa aatgttgagc tagtttgcgc tttcattcag aaaacatctg
     7441 ctgaaaaggc agccgcagag atcgataggc gtctaagcac cgatttcgag accagaaaaa
     7501 ttgctcgtga agagggcaac cgctttgtgg acgcccagat cctcacctat caacaggagc
     7561 gtcttccaga agcagtgcgc atcaaggtgg gcgcggctcc agctacactt tatgctgtgt
     7621 actcggagtt cgctagaagc atacctggct tccagcagat gagcgatcgc gatattgccc
     7681 tgtttgtgcc caagccgacg gatttgtctc agccaaatgt gtttgctaat gatgatagca
     7741 tggtatacgg ggaactggct agcaaaatgg aggccttcat gaacacggca atcggagtgc
     7801 cgacactcca gatccaggct agcaagatgc acatgctact caacgccctg atagcgacgc
     7861 gtcgactgcg tgaccaagag tctgccttta atctgctgac ccgcgcggtc gagggtttga
     7921 ccgaaggatt ggtcaatatg catgaaaaca tggagcaaat gaagatgtac caaaacatcc
     7981 atctccgtat ccttggcttg ctgaacaaca gtttcggtgc tccaaacaca gagcgagcgg
     8041 taacaaagtg tttcttcgat atccgagagg aggtgcgata taacgtggag gcagctcgcg
     8101 ctttgattac gtcgcacttt gtcaatctga atcagtttga cggaatgcta cgcgactgca
     8161 tggacaacgg aaacaattat gtggctatat cgttcggtat cgcactgctg gagcgcctca
     8221 tcatggacga tcgagtgatt aatattgttt cggacaacga attcatggca actgttgagc
     8281 tgctaggtag gctcactcag catcgccatc ggtatccaga atgcattgtg aatgctattg
     8341 atacgctctg gagcggaaac tttaactcga gcagcgacta cagcccgttc aacggcaatg
     8401 atcgttactt gtcgggagcc tcccattaca tccactccgg catgcatcac gtaaggtcat
     8461 gcgatacaga tgatccgccg ggcttacaag agaagacgga gtttctgctt aaggattggg
     8521 ttgcactgta cacgcaacag aaccagcagt caacgcgcga tgcacgcaac ttcagtgcat
     8581 ttgttcagaa gatgaacaca tatggtatcc tgaaaacgga cgacttgatc acccgttttt
     8641 tccgccaggc cacacacatt tgcacggatg tggtgtacag aatgttcgcc gagccgagtt
     8701 taccaatcaa ccaggccaaa aacaagatat tccagtggat cgatgcgttc gtccatctga
     8761 ttgcaatgct cgtgcgacac tcaggcgagg caggtaatcc aaccaccaag atcaacctgt
     8821 taaacaaggt gctgggcatt gtgctgggaa cgctgattaa ggaccatgag atgcgtggcg
     8881 ttagcttcca gcaggttggc taccatcgct tcttcatgat gctgttcatg gagctgtgca
     8941 cggccgatgt gattctcgag tccctgatgc acagtattgt gtcggccttc gcctacacat
     9001 atcacttgtt gaatcccagc gtggctccgg gattctgctt tgcttggttg gagctcattt
     9061 cgcatcgcgt cttcctgggc cgcatcctgg tccaaattcc cgggcaaaag ggctggccac
     9121 tttacgctca attgctgcag gaccttttta aatatcttgc gcccttctta cgcaacactg
     9181 agctcggcaa accagtccag cttttgtaca aggggatgct acgtgttctg ctcgttctgc
     9241 ttcacgactt cccggagttc ttgtgcgact atcattttgg gttctgtgac accatcccgc
     9301 ccaattgcgt ccagatgcgt aacatcatcc tgtcggcgtt tccgcgcaac atgcgcttgc
     9361 ccgatccctt cacgcccaac ctaaaagtgg acatgctgtc ggacagtagt aatgcgccta
     9421 aggtgctcag tagctacatt atgaacatcc agcccgcgaa ctttaagaag gatttggact
     9481 cgtacctcaa ggcccgggcg ccagttactt ttctatcgga attacgtggt cacttgcagg
     9541 tgacaagcga gccaggaact cgttacaaca tggcgctgat gaatgctctg gtcatgtatg
     9601 tgggaactca ggcgattgct ttgatcagaa acaaaaactt tgtgcccaac acctcgaaca
     9661 tagcacacag cgcccacatg gatatcttcc agaatcttgc tgttgacttg gatacggagg
     9721 gtcgctatct gttcttgaat gcgattgcga accagttgcg ctatcccaac agccacacgc
     9781 actacttcag ctgtgcggtg ctgcatctgt ttgccgaggc caactcggag gcaattcagg
     9841 agcagatcac tcgcgtactt ctggaacgat tgatcgtgaa ccgcccgcat ccttggggac
     9901 tgttgatcac ttttattgag ctgatcaaaa atcccatcta caagttctgg gaccatgact
     9961 ttgtgcactg tgcgccagag atcaccaagc tctttgaatc ggtggcccgt tcctgtctgg
    10021 ccaagtcgaa cgtcacccag cagttgaaca tgcccgtcgt cgatggcgag ggccaggagg
    10081 tagccaccat caacgcatcc agcggcggta ccaacatggt gagcaagggc gaggaggata
    10141 acatggccat catcaaggag ttcatgcgct tcaaggtgca catggagggc tccgtgaacg
    10201 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
    10261 ccaagctgaa ggtgaccaag ggtggccccc tgcccttcgc ctgggacatc ctgtcccctc
    10321 agttcatgta cggctccaag gcctacgtga agcaccccgc cgacatcccc gactacttga
    10381 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggcg
    10441 tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac aaggtgaagc
    10501 tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc atgggctggg
    10561 aggcctcctc cgagcggatg taccccgagg acggcgccct gaagggcgag atcaagcaga
    10621 ggctgaagct gaaggacggc ggccactacg acgctgaggt caagaccacc tacaaggcca
    10681 agaagcccgt gcagctgccc ggcgcctaca acgtcaacat caagttggac atcacctccc
    10741 acaacgagga ctacaccatc gtggaacagt acgaacgcgc cgagggccgc cactccaccg
    10801 gcggcatgga cgagctgtac aagccaagct tgaattctgc agatggctcc atgcacggtc
    10861 tccaggatga tccagatctg caggctctgc tgaaaggttc tcagctcctc aaagtcaaaa
    10921 gcagctcctg gcgtcgtgaa cgcttctaca aactgcagga ggactgcaaa accatctggc
    10981 aggaatcccg taaagtaatg cgctccccag agtctcagct cttcagcatt gaggacattc
    11041 aggaggtacg tatgggtcac cgtaccgaag gtctggagaa attcgctcgc gatatcccag
    11101 aagatcgctg cttctctatc gtcttcaaag accagcgcaa caccctggat ctgattgctc
    11161 catctccagc agatgctcag cactgggttc agggtctccg caaaatcatt caccactccg
    11221 gttctatgga ccagcgtcag aaataaatcc agcacagtgg cggccgctcg agtctagagg
    11281 gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat tctacgcgta
    11341 ccggtcatca tcaccatcac cattgagttt aaacccgctg atcagcctcg actgtgcctt
    11401 ctaaggcctg agctcgctga tcagcctcga tcgaggatcc agacatgata agatacattg
    11461 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
    11521 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt aacaacaaca
    11581 attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt taaagcaagt
    11641 aaaacctcta caaatgtggt atggctgatt atgatcagtc gaccgatgcc cttgagagcc
    11701 ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg
    11761 actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc
    11821 ggcgaggacc gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga
    11881 atcttgcacg ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag
    11941 aagcaggcca ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc
    12001 gcgacgcgag gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg
    12061 atgcccgcgt tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt
    12121 caaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
    12181 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
    12241 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
    12301 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
    12361 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
    12421 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
    12481 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
    12541 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
    12601 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
    12661 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
    12721 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
    12781 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
    12841 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
    12901 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
    12961 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
    13021 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
    13081 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
    13141 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
    13201 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctgcagg catcgtggtg
    13261 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
    13321 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
    13381 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
    13441 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
    13501 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaacacg ggataatacc
    13561 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
    13621 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
    13681 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
    13741 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
    13801 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
    13861 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
    13921 gacgtctaag aaaccattat tatcatgaca ttaacctata aaaataggcg tatcacgagg
    13981 ccctttcgt
//
