LOCUS       sequence-407840-        8927 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Roquin expression in Schneider cells for imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8927)
  AUTHORS   Salgania HK, Metz J, Jeske M
  TITLE     ReLo is a simple and rapid colocalization assay to identify and 
            characterize direct protein-protein interactions.
  JOURNAL   Nat Commun. 2024 Apr 3;15(1):2875. doi: 10.1038/s41467-024-47233-4.
  PUBMED    38570497
REFERENCE   2  (bases 1 to 8927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-04-3"; volume: "15(1):2875. doi"; pages: " 
            10.1038/s41467-024-47233-4"
COMMENT     Sequence Label: pAc5.1-PH-mCherry-Roquin isoform A (HK63)
FEATURES             Location/Qualifiers
     source          1..8927
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..2565
                     /label=Ac5 promoter
                     /note="Drosophila melanogaster actin 5C promoter"
     CDS             2984..3691
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(3129..3147)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     3397..3416
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             6233..6274
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6284..6301
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(6324..6341)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6374..6508
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6398..6417)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     6452..6471
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(7109..7697)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7189..7208)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7868..8659,8660..8728))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8491..8510
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8729..8833)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8901..8919
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 cttcaagaat taattctata ttctaaaaac acaaatgata cttctaaaaa aaaatcatga
       61 atggcatcaa ctctgaatca aatctttgca gatgcaccta cttctcattt ccactgtcac
      121 atcatttttc cagatctcgc tgcctgttat gtggcccaca aaccaagaca cgttttatgg
      181 ccattaaagc tggctgatcg tcgccaaaca ccaaatacat aatgaatatg tacacattcg
      241 agaaagaagc gatcaaagaa gcgtcttcgg gcggagtagg agaatgcgga ggagaaggag
      301 aacgagctga tctagtatct ctccacaatc caatgccaac tgaccaactg gccatattcg
      361 gagcaatttg aagccaattt ccatcgcctg gcgatcgctc cattcttggc tatatgtttt
      421 tcaccgttac ccggggccat tttcaaagac tcgtcggcaa gataagattg tgtcactcgc
      481 tgtctctctt catttgtcga agaatgctga ggaatttcgc gatgacgtcg gcgagtattt
      541 tgaagaatga gaataatttg tatttatacg aaaatcagtt agtggaattt tctacaaaaa
      601 catgttatct atagataatt ttgttgcaaa atatgttgac tatgacaaag attgtatgta
      661 tataccttta atgtattctc attttcttat gtatttataa tggcaatgat gatactgatg
      721 atattttaag atgatgccag accaaaaggc ttgaatttct gcgtcttttg ccgaacgcag
      781 tgcatgtgca attgttgttt tttggaatat tcaattttcg gactgtccgc tttgatttca
      841 gtttcttggc ttattcaaaa agcaaagtaa agccaaaaaa gcgagatggc aataccaaat
      901 gcggcaaaac ggtagtggaa ggaaaggggt gcggggcagc ggaaggaagg gtggggcggg
      961 gcgtggcggg gtctgtggct gggcgcgacg tcaccgacgt tggagccact cctttgacca
     1021 tgtgtgcgtg tgtgtattat tcgtgtctcg ccactcgccg gttgtttttt tctttttatg
     1081 ctgcgctctc tctagcgcca tctcgcttac gcatgctcaa cgcaccgcat gttgccgttt
     1141 ccttttatgc gtcattttgg ctcgaaatag gcaattattt aaacaaagat tagtcaacga
     1201 aaacgctaaa ataaataagt ctacaatatg gttacttatt gccatgtgtg tgcagccaac
     1261 gatagcaaca aaagcaacaa cacaggtggc tttccctctt tcactttttg tttgcaagcc
     1321 gcgtgcgagc aagacggcac gaccggcaaa cgcaattacg ctgacaaaga gcagacgaag
     1381 ttttggcgaa aaacatcaag gcgcctgata cgaatgcatt tgcaataaca attgcgatat
     1441 ttaatattgt ttatgaagct gtttgacttc aaaacacaca aaaaaaaaaa taaaacaaat
     1501 tatttgaaag agaattagga atcggacgct tatcgttagg gtaacaacaa gaaatgctta
     1561 ctgagtcaca gcctctggaa aactgccgca agccagagag agagagaaaa agagggagag
     1621 cagcttagac cgcatgtgct tgtgtgtgag gcgtctctct cttcgtctct gttgcgcaaa
     1681 cgcatagact gcactgaaaa aatcgattac ctatttttta tgaatgaata tttgcactat
     1741 tactattcaa aactattaag atagcaatca cattcaatag ccaaatacta taccacctga
     1801 gcgatgcaac gaaatgatca atttgagcaa aaatgctgca tatttaggac ggcatcatta
     1861 tagaaatgct tcttgctgtg tacttttctc tcgtctggca gctgtttcgc cgttattgtt
     1921 aaaaccggct taagttaggt gtgttttcta cgactagtga atgccctact agaagatgtg
     1981 tgttgcacaa aatgtccctg gaataaccaa tttgaagtgc agatagcagt aaacgtaagc
     2041 taatatgaat attatttaac tgtaatgttt taatatcgct ggacattact aataaaccca
     2101 ctataaacac atgtacatat gtatgttttg gcatacaatg agtagttggg gaaaaaatgt
     2161 gtaaaagcac cgtgaccatc acagcataaa gataaccagc tgaagtatcg aatatgagta
     2221 acccccaaat tgaatcacat gccgcaactg ataggaccca tggaagtaca ctcttcatgg
     2281 cgatatacaa gacacacaca agcacgaaca cccagttgcg gaggaaattc tccgtaaatg
     2341 aaaacccaat cggcgaacaa ttcataccca tatatggtaa aagttttgaa cgcgacttga
     2401 gagcggagag cattgcggct gataaggttt tagcgctaag cgggctttat aaaacgggct
     2461 gcgggaccag ttttcatatc actaccgttt gagttcttgt gctgtgtgga tactcctccc
     2521 gacacaaagc cgctccatca gccagcagtc gtctaatcca gagaccccgg atcggggtac
     2581 caacatgcac ggtctccagg atgatccaga tctgcaggct ctgctgaaag gttctcagct
     2641 cctcaaagtc aaaagcagct cctggcgtcg tgaacgcttc tacaaactgc aggaggactg
     2701 caaaaccatc tggcaggaat cccgtaaagt aatgcgctcc ccagagtctc agctcttcag
     2761 cattgaggac attcaggagg tacgtatggg tcaccgtacc gaaggtctgg agaaattcgc
     2821 tcgcgatatc ccagaagatc gctgcttctc tatcgtcttc aaagaccagc gcaacaccct
     2881 ggatctgatt gctccatctc cagcagatgc tcagcactgg gttcagggtc tccgcaaaat
     2941 cattcaccac tccggttcta tggaccagcg tcagaaaggt accatggtga gcaagggcga
     3001 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     3061 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     3121 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     3181 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     3241 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     3301 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     3361 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     3421 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     3481 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     3541 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     3601 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     3661 ctccaccggc ggcatggacg agctgtacaa gccaagcttg aattctgcaa cgtgcatgcc
     3721 gattcaggct ccctcatgga cagattttct gaactgtccc atctgctgca atgaattcgc
     3781 ggccagccag cgatgtccgg tcagtctggg ctgcgggcac accatctgca agctatgcct
     3841 gacgaccctc tacaaccggc agtgtccctt cgatcagacc gtgattgtca gtgacatcga
     3901 caatttgccc atcaaccatg ccttgctcca gttggtcaag gactcggagc tcctcgagct
     3961 ggcaccacca ccgcccagtg tgcagaagct ggagccggag caccttaagt gttaccaact
     4021 gggtcagcgg tgcatcgagg agctggcttt gcacttgaag tccttcctca acctgaacgg
     4081 aaatggcaat cttctaactc ggcccatgtt acggaaactg gttactctgg tcaactgcca
     4141 gctgatggag gaggagggac gggtgagagc gctccgagca gccagatccc taggcgaacg
     4201 cacagtcacc gagctgatcc tgcagcacca aaatccccag cagttgagct caaatctgtg
     4261 ggctgcagtg cggacaagag gatgccagtt ccttggaccc gccatgcagg aggaggtgct
     4321 caaactggtg ctgctggcct tggaggaggg atcagcgctt tcgcgcaaag tgctggtcat
     4381 gttcgtcgtg cagcgactag agccgaagtt tccccaggcc tccaagacga gtatcggaca
     4441 tgtggtacag ctgctctacc gcgccagctg cttcaaggtg tccaagcggg aggcggactc
     4501 gtcgttgatg cagctcaaag aggaattccg tacgtatgac gccctacgcc gggagcacga
     4561 tgcccaaatc gttcagatag ccaccgaggc gggtctgcga attgcaccgg aacaatggtc
     4621 ttccctattg tatggcgacg tgatgcataa aagccacatg cagagcatca tcgataagct
     4681 gcagacgcca agctcctttg cgcaatcagt ccaggaattg gtcatcgcct tgcagcggac
     4741 cagcgatcct gcgaagctat ccagtttgca tcatcatctg aagtacctgg ccaatattga
     4801 tccctgtgcg gaggttgccc cgtggtcgga tttggctgag gccttggatg cagtgcggca
     4861 ttcggtcgtt ggactggtga acttccttca gcaccacgga gtgcgcaagg cgcaggacgg
     4921 catcagtggc ggtggcagtg gtgggtcgac caatagcaat ccgaagtaca agatcagctt
     4981 atgcagggac ttgaatgtgc ggcgggtgtg tccccgaggt tctagctgta cctttgccca
     5041 ttcccaggag gaggtagaac gctatcgtgc acggaatcga ggaaaacata tgaagacacc
     5101 gttggcgctg cagggacctc cagcggtagg cgtaggagcc ataaagaaac cactaggcga
     5161 gcaggaggga ccgcctctag gtaacatgcc acccatgtta cctatgtcgc ctatgcatta
     5221 tatgggctct cctaggggat atctggatcc cagcttggga ctttcgccag gtggtggtct
     5281 cccgccgtcg catcacagtc ccattaccag gctcatagtg ccgagccgct acgattcgcg
     5341 ctttagcggg tttggcggtg gcacgcccag gattccttcg cccagagaat accaagccaa
     5401 tccggtagcg ccaacgcaac gcaatgccaa cccaccgaat tttagtgtga acagtaacct
     5461 gcacaagggc tacatgttgc ccgccagcgg tggagatgtc ttccatctgg ccaatccgtg
     5521 ggagcaagca tacctggcac agcagcaaca tcctccacag catccccaac agcagcagcc
     5581 accgtccagc aagccgaatc ccagccgacc gttgtcgatt ttacccgcaa cagctgatac
     5641 ctcatttttt gaaaagaaac cgccgaatag tgttagcata gatctggaca gggtcccgga
     5701 ggtcaatgtg gatgcagtgc ctctgttccg gcgatccaac aataaccaca ataacaacaa
     5761 tagtaactcc cacaataaca acaacaacaa tcatggctca tcactgctgt tctggaacaa
     5821 tacgggtaaa gattcagcca actttgtacg atcagactcc atattggatg acgatgcgtc
     5881 cacattcgat gtgcccactg gctcctccat gctgagtatc tacggcccga tttgccccaa
     5941 gagcagtacg accggcaact ggaacaactt tgatctggga tatggcggct tcaagagtga
     6001 cagaaacgat agcttcaatg ccaataagca gcaacagccg atgtggggca ggaaaccgca
     6061 actgatgtcg gaagacagtt tcgagggcgg catcgatagt ggaatgatgc tgcagctgga
     6121 gaagaatctg gtggacatcg tcgatcccga tgacagtggg atcaaggtgg actaggcatg
     6181 agatatccag cacagtggcg gccgctcgag tctagagggc ccgcggttcg aaggtaagcc
     6241 tatccctaac cctctcctcg gtctcgattc tacgcgtacc ggtcatcatc accatcacca
     6301 ttgagtttaa acccgctgat cagcctcgac tgtgccttct aaggcctgag ctcgctgatc
     6361 agcctcgatc gaggatccag acatgataag atacattgat gagtttggac aaaccacaac
     6421 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
     6481 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
     6541 ggttcagggg gaggtgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtat
     6601 ggctgattat gatcagtcga ccgatgccct tgagagcctt caacccagtc agctccttcc
     6661 ggtgggcgcg gggcatgact atcgtcgccg cacttatgac tgtcttcttt atcatgcaac
     6721 tcgtaggaca ggtgccggca gcgctctggg tcattttcgg cgaggaccgc tttcgctgga
     6781 gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat cttgcacgcc ctcgctcaag
     6841 ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa gcaggccatt atcgccggca
     6901 tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc gacgcgaggc tggatggcct
     6961 tccccattat gattcttctc gcttccggcg gcatcgggat gcccgcgttg caggccatgc
     7021 tgtccaggca ggtagatgac gaccatcagg gacagcttca aggccagcaa aaggccagga
     7081 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     7141 acaaaaatcg atgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     7201 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     7261 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     7321 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     7381 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     7441 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     7501 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     7561 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     7621 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     7681 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     7741 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     7801 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     7861 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     7921 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     7981 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     8041 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     8101 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     8161 tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     8221 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     8281 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     8341 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     8401 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     8461 cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     8521 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     8581 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     8641 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     8701 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     8761 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     8821 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     8881 tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgt
//
