LOCUS       sequence-407848-       11587 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11587)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11587)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1041
FEATURES             Location/Qualifiers
     source          1..11587
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7444..8032
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7497..7517)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7915..7926)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8104..8337
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8415..8536
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8452..8471)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8506..8525
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8576..8711
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8731..8750)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8732..8750)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8760..8777)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8760..8776)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8769..8791)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8918..9373
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9005..9024)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9215..9236
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9399..9503
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9504..9572,9573..10364)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9722..9741)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10535..11123
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11024..11043
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11277..11294
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11411..11432
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11447..11452,11453..11470,11471..11477)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11485..11501
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11490..11512
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11509..11525
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11509..11525
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11544..11564
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11546..11564
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgcac cagccgcctg agtccaccgc
     4141 cgcggccgcc gccgctgcag acattagcgc taggaagatg gcgcacccgg caatgttccc
     4201 tcgaaggggc agcggtagtg gcagcgcctc tgctctcaat gcagcaggta ccggcgtcgg
     4261 tagtaatgcc acatcttccg aggattttcc gcctccgtcg ctgcttcagc cgccgccccc
     4321 tgcagcatct tctacgtcgg gaccacagcc tccgcctcca caaagcctga acctcctttc
     4381 gcaggctcag ctgcaggcac agcctcttgc gccaggcgga actcaaatga aaaagaaaag
     4441 tggcttccag ataactagcg ttactcctgc tcagatctcc gctagtatca gctctaacaa
     4501 cagtatagca gaggacactg agagctatga tgatctggat gaatctcaca cggaagatct
     4561 ctcttcttcg gagatccttg atgtgtcact ttccagggct actgacttag gggagcccga
     4621 acgcagctcc tcagaagaga ccctaaataa cttccaggaa gccgagacac ctggggcagt
     4681 ctctcccaac cagccccacc ttcctcagcc tcatttgcct caccttccac aacagaatgt
     4741 tgtgatcaat gggaatgctc atccacacca cctccatcac caccatcaga ttcatcatgg
     4801 gcaccacctc caacatggtc accaccatcc atctcatgtt gctgtggcca gtgcatccat
     4861 tactggtggg ccaccctcaa gcccagtatc tagaaaactc tctacaactg gaagctctga
     4921 cagtatcaca ccagttgcac caacttctgc tgtatcatcc agtggttcac ctgcatctgt
     4981 aatgactaat atgcgtgctc caagtactac aggtggaata ggtataaatt ctgttactgg
     5041 cactagtaca gtaaataatg ttaacattac tgctgtgggt agttttaatc ctaatgtgac
     5101 aagcagcatg cttggtaatg ttaatataag tacaagcaat attcctagtg ctgctggtgt
     5161 gagtgttggg cctggagtta ccagtggtgt taatgtgaat atcttgagtg gcatgggcaa
     5221 tggtactatt tcttcctctg ctgctgttag cagtgttcct aatgcagctg cagggatgac
     5281 tgggggatcg gtttcaagtc agcagcaaca accaacagtt aacacttcga ggttcagagt
     5341 tgtgaagtta gattctagtt ctgagccctt taaaaaaggt agatggactt gcactgagtt
     5401 ctatgaaaaa gaaaatgctg tacctgctac agaaggtgtg ctgataaata aagtggtgga
     5461 gactgtaaag caaaatccga tagaagtgac ttctgaaagg gagagcacta gtgggagttc
     5521 agtgagcagt agtgtcagca cactgagtca ctatacagag agtgtgggaa gtggagagat
     5581 gggagcccct actgtggtgg tgcagcagca gcagcagcaa caacaacaac aacagcaaca
     5641 accagctctc caaggtgtga ccctccaaca gatggatttt ggtagcactg gtccacagag
     5701 tattccagca gttagtatac cacagagtat ttctcagtca cagatctcac aagtacaatt
     5761 acagtctcaa gaactgagct atcagcaaaa gcaaggtctt cagccagtac ctctgcaagc
     5821 cactatgagt gctgcaactg gtatccagcc atcgcctgta aatgtggttg gtgtaacttc
     5881 agctttaggt cagcagcctt ccatttccag tttggctcaa ccccagctac catattctca
     5941 ggcggctcct ccagtgcaaa ctccccttcc aggggcacca ccaccccaac agttacagta
     6001 tggacaacag caaccaatgg tttctacaca gatggcccca ggccatgtca aatcagtgac
     6061 tcaaaatcct gcttcagagt atgtacaaca gcagccaatt cttcaaacag caatgtcctc
     6121 cggacagccc agttctgcag gagtaggagc aggaacaaca gtgattcctg tggctcagcc
     6181 acagggtatc cagctgccag tgcagcccac agcagtccca gcacaacctg caggggcatc
     6241 tgtccagcct gttggccagg ctccggcagc agtgtctgct gtacctactg gcagtcagat
     6301 tgcaaatatt ggtcagcaag caaacatacc tactgcagtg cagcagccct ctacccaggt
     6361 tccaccttca gttattcagc agggtgctcc tccatcttcg caagtggttc cacctgctca
     6421 aactgggatt attcatcagg gagttcaaac tagtgctcca agccttcctc aacaattggt
     6481 tattgcatcc caaagttcct tgttaactgt gcctccccag ccacaaggag tagaaccagt
     6541 agctcaagga attgtttcac agcagttgcc tgcagttagt tctttgccct ctgctagtag
     6601 tatttctgtt acaagtcagg ttagttcaac tggtccttct ggaatgcctt ctgccccaac
     6661 aaacttggtt ccaccacaaa atatagcaca aacccctgct acccaaaatg gtaatttggt
     6721 tcaaagtgtt agtcaacctc ccttgatagc aactaataca aatttgcctt tggcacaaca
     6781 gataccacta agttctaccc agttctccgc acaatcatta gctcaggcaa ttggaagcca
     6841 aattgaagat gccaggcgtg cagcggagcc ctccttagtt ggcttacctc agactatcag
     6901 tggtgacagt gggggaatgt cagcagtttc agatgggagt agcagcagcc tagcagcctc
     6961 tgcttctctt ttcccgttga aggtgctacc gctgacgaca cccctggtgg atggcgagga
     7021 tgagagctcc tctggtgcaa gtgtggtagc tattgacaac aaaatcgagc aagctatgga
     7081 tctagtgaaa agccatttga tgtatgcggt cagagaagaa gtggaggtcc tcaaagagca
     7141 aatcaaagaa ctaatagaga aaaattccca gctggagcag gagaacaatc tgctgaagac
     7201 actggccagt cctgagcagc ttgcccagtt tcaggcccag ctgcagactg gctccccccc
     7261 tgccaccacc cagccacagg gcaccacaca gccccccgcc cagccagcat cgcagggctc
     7321 aggaccaacc gcatagtaat gaactagtcc gtaacttgaa agtatttcga tttcttggct
     7381 ttatatatct tgtggaaagg acgagtagat cgcctggacg gtcagtcaac gcgttaagtc
     7441 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7501 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7561 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7621 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7681 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7741 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7801 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     7861 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7921 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     7981 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcgtcgactt
     8041 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     8101 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     8161 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     8221 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     8281 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta
     8341 cgtatagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     8401 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     8461 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     8521 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     8581 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8641 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8701 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     8761 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     8821 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     8881 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     8941 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     9001 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     9061 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     9121 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     9181 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     9241 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     9301 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     9361 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     9421 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     9481 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     9541 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     9601 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     9661 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     9721 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     9781 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     9841 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     9901 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     9961 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
    10021 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
    10081 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
    10141 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
    10201 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
    10261 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
    10321 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
    10381 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
    10441 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
    10501 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
    10561 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
    10621 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
    10681 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
    10741 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
    10801 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
    10861 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
    10921 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
    10981 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
    11041 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
    11101 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
    11161 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
    11221 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
    11281 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
    11341 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
    11401 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
    11461 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
    11521 atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa aagctggagc
    11581 tgcaagc
//
