LOCUS       sequence-407857-       11179 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11179)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11179)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1085
FEATURES             Location/Qualifiers
     source          1..11179
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7036..7624
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7089..7109)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7507..7518)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7696..7929
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8007..8128
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8044..8063)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8098..8117
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8168..8303
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8323..8342)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8324..8342)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8352..8369)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8352..8368)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8361..8383)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8510..8965
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8597..8616)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8807..8828
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8991..9095
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9096..9164,9165..9956)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9314..9333)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10127..10715
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10616..10635
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10869..10886
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11003..11024
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11039..11044,11045..11062,11063..11069)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11077..11093
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11082..11104
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11101..11117
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11101..11117
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11136..11156
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11138..11156
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgact gactccaagt atttcacaac
     4141 caataaaaaa ggagaaatat ttgaactaaa agctgaactc aacaatgaaa agaaagaaaa
     4201 gagaaaggag gctgtgaaga aagtgattgc tgctatgacc gtggggaagg atgttagttc
     4261 tctctttcca gacgtagtga actgtatgca gactgacaat ctggaactaa agaagcttgt
     4321 gtatctctac ttgatgaact acgccaagag tcagccagac atggccatca tggctgtaaa
     4381 cagctttgtg aaggactgtg aagatcctaa tcctttgatt cgagccttgg cagtcagaac
     4441 catggggtgc atccgggtag acaaaattac agaatatctc tgtgagccgc tccgcaagtg
     4501 cttgaaggat gaggatccct atgttcggaa aacagcagca gtctgcgtgg caaaactcca
     4561 tgatatcaat gcccaaatgg tggaagatca gggatttctg gattctctac gggatctcat
     4621 agcagattca aatccaatgg tggtggctaa tgccgtagcg gcattatctg aaatcagtga
     4681 gtctcaccca aacagcaact tacttgatct gaacccacag aacattaata agctgctgac
     4741 agccctgaat gaatgcactg aatggggcca gattttcatc ctggactgcc tgtctaatta
     4801 caaccctaaa gatgatcggg aggctcagag catctgtgag cgggtaactc cccggctatc
     4861 ccatgccaac tcagcagtgg tgctttcagc ggtaaaagtc ctaatgaagt ttctagaatt
     4921 gttacctaag gattctgact actacaatat gctgctgaag aagttagccc ctccacttgt
     4981 cactttgctg tctggggagc cagaagtgca gtatgtcgcc ctgaggaaca tcaacttaat
     5041 tgtccagaaa aggcctgaaa tcttgaagca ggaaatcaaa gtcttctttg tgaagtacaa
     5101 tgatcccatc tatgttaaac tagagaagtt ggacatcatg attcgtttgg catctcaagc
     5161 caacattgct caggttctgg cagaactgaa agaatatgct acagaggtgg atgttgactt
     5221 tgttcgaaaa gctgtgcggg ccattggacg gtgtgccatc aaggtggagc aatctgcaga
     5281 gcgctgtgta agcacattgc ttgatctaat ccagaccaaa gtgaattatg tggtccaaga
     5341 agcaattgtt gtcatcaggg acatcttccg caaatacccc aacaagtatg aaagtatcat
     5401 cgccactctg tgtgagaact tagactcgct ggatgagcca gatgctcgag cagctatgat
     5461 ttggattgtg ggagaatatg ctgaaagaat tgacaatgca gatgagttac tagaaagctt
     5521 cctggagggt tttcacgatg aaagcaccca ggtgcagctc actctgctta ctgccatagt
     5581 gaagctgttt ctcaagaaac catcagaaac acaggagcta gtccagcagg tcttgagttt
     5641 ggcaacacag gattctgata atcctgacct tcgagaccgg ggctatattt attggcgcct
     5701 tctctcaact gaccctgtta cagctaaaga agtagtcttg tctgagaagc cactgatctc
     5761 tgaggagacg gaccttattg agccaactct gctggatgag ctaatctgcc acattggttc
     5821 tttggcctct gtgtatcata agcctcccaa tgcttttgtg gaaggaagtc atggaattca
     5881 tcgtaaacac ttgccaattc atcatgggag cactgatgca ggtgacagcc ctgttggcac
     5941 taccactgca acgaacctgg aacagcctca ggttatcccc tctcaaggtg atcttctagg
     6001 ggatctttta aaccttgacc tcggtccccc agtcaatgtg ccacaggtgt cctccatgca
     6061 gatgggagca gtggatctcc taggaggagg actagatagt ctggtgggac aatccttcat
     6121 cccatcatcg gtgcctgcaa cctttgctcc ttcacctaca cctgctgtgg tcagcagtgg
     6181 actgaatgac ctgtttgaac tctccacagg gataggcatg gcacctggtg gatatgtggc
     6241 tcctaaggct gtctggctac ctgcagtaaa ggctaaaggc ttggagattt ccggaacatt
     6301 tactcaccgc caagggcaca tctatatgga aatgaacttc accaataaag ctctgcagca
     6361 catgacagat tttgcaatcc agtttaacaa aaatagcttt ggtgtcatcc ccagcactcc
     6421 tctggccatc catacaccac tgatgccaaa ccagagcatt gatgtctccc tgcctctcaa
     6481 taccttgggc ccagtcatga agatggaacc tctgaataac ctccaggtgg ctgtgaaaaa
     6541 caatatcgat gtcttctact tcagctgcct catcccactc aatgtgcttt ttgtagaaga
     6601 tggcaaaatg gagcgccagg tcttccttgc aacatggaag gatattccca atgaaaatga
     6661 acttcagttt cagattaagg aatgtcattt aaatgctgac actgtttcca gcaagttgca
     6721 aaacaacaat gtttatacta ttgccaagag gaatgtggaa gggcaggaca tgctgtacca
     6781 atccctgaag ctcactaatg gcatttggat tttggccgaa ctacgtatcc agccaggaaa
     6841 ccccaattac acgctgtcac tgaagtgtag agctcctgaa gtctctcaat acatctatca
     6901 ggtctacgac agcattttga aaaactagta atgaactagt ccgtaacttg aaagtatttc
     6961 gatttcttgg ctttatatat cttgtggaaa ggacgaggag ggtgccgatg acccatcaaa
     7021 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     7081 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     7141 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     7201 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     7261 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     7321 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     7381 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     7441 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     7501 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     7561 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     7621 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     7681 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     7741 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     7801 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     7861 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     7921 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     7981 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     8041 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     8101 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     8161 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     8221 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     8281 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     8341 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     8401 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     8461 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     8521 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     8581 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     8641 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     8701 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     8761 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     8821 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     8881 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     8941 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     9001 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     9061 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     9121 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     9181 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     9241 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     9301 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     9361 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     9421 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     9481 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     9541 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     9601 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     9661 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     9721 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     9781 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     9841 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     9901 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     9961 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
    10021 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
    10081 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
    10141 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
    10201 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
    10261 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
    10321 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
    10381 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
    10441 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
    10501 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
    10561 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
    10621 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
    10681 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
    10741 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
    10801 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
    10861 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
    10921 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
    10981 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
    11041 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
    11101 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
    11161 aaaagctgga gctgcaagc
//
