LOCUS       sequence-407884-       11698 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11698)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11698)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0634
FEATURES             Location/Qualifiers
     source          1..11698
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4109..4118
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(5931..5942)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    7555..8143
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7608..7628)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8026..8037)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8215..8448
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8526..8647
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8563..8582)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8617..8636
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8687..8822
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8842..8861)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8843..8861)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8871..8888)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8871..8887)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8880..8902)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9029..9484
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9116..9135)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9326..9347
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9510..9614
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9615..9683,9684..10475)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9833..9852)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10646..11234
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11135..11154
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11388..11405
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11522..11543
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11558..11563,11564..11581,11582..11588)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11596..11612
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11601..11623
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11620..11636
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11620..11636
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11655..11675
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11657..11675
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatggcg gaaaacaaag gcggcggcga
     4141 ggctgagagc ggcggcgggg gcagcggcag cgcgccggta actgccgggg ccgccgggcc
     4201 cgccgcgcag gaggcggagc cgcctctcac cgcggtgctg gtggaggagg aggaggagga
     4261 aggcggcagg gccggcgctg agggcggcgc ggccgggccc gacgacgggg gggtggccgc
     4321 ggcctcctcg ggctcggccc aggctgcttc atctcctgcg gcctcagtgg gcactggagt
     4381 tgccgggggc gcagtatcga cgccggctcc agctccagcc tcggctcccg ctccgggtcc
     4441 ctcggcaggg ccgcctcctg gaccgccagc ctcgctcctg gacacctgcg ccgtgtgtca
     4501 gcagagcttg cagagccggc gtgaggcgga gcccaagctg ctgccctgtc ttcactcctt
     4561 ctgcctgcgc tgcctgcccg agccggagcg ccagctcagc gtgcccatcc cggggggcag
     4621 caacggcgac atccagcaag ttggtgtaat acggtgccca gtatgccgcc aagaatgcag
     4681 acagatagac cttgtggata attattttgt gaaagacaca tctgaagctc ctagcagttc
     4741 tgatgaaaaa tcagaacagg tatgtactag ttgtgaagac aatgcaagtg cagttggctt
     4801 ttgtgtagaa tgtggagagt ggctatgtaa gacatgtatc gaagcacatc aaagagtaaa
     4861 atttactaaa gatcacttga tcaggaagaa agaagatgtc tcagagtctg ttggagcatc
     4921 tggtcaacgc cctgttttct gccctgtaca caaacaagaa cagttgaaac ttttctgtga
     4981 aacatgtgat agattgacat gtagagactg tcagctattg gaacacaaag aacataggta
     5041 tcagtttttg gaagaagctt ttcaaaatca gaagggtgca attgagaatc tactggcgaa
     5101 acttcttgag aagaagaatt atgttcattt tgcagctact caggtgcaga ataggataaa
     5161 agaagtaaat gagactaaca aacgagtaga acaggaaatt aaagtggcca ttttcaccct
     5221 tatcaatgaa attaataaga aaggaaaatc tctcttacaa cagctagaga atgttacaaa
     5281 ggaaagacag atgaagttac tacagcagca gaatgacatc acaggccttt cccggcaggt
     5341 gaagcatgtt atgaacttca caaattgggc aattgcaagt ggcagcagca cagcactact
     5401 atacagcaag cgactgatta ctttccagtt gcgtcatatt ttgaaagcac ggtgtgatcc
     5461 tgtccctgct gctaatggag caatacgttt ccattgtgat cccaccttct gggcaaagaa
     5521 tgtagtcaat ttaggtaatc tagtaataga gagtaaacca gctcctggtt atactcctaa
     5581 tgttgtagtt gggcaagttc ctccagggac aaaccacatt agtaaaaccc ctggacagat
     5641 taacttagca cagcttcgac tccagcacat gcaacaacaa gtatatgcac agaaacatca
     5701 gcagttgcaa cagatgagga tgcagcaacc accagcacct gtaccaacta caacaacaac
     5761 aacacaacag catcctagac aagcagcccc tcagatgtta caacaacagc ctcctcgatt
     5821 gatcagtgtg caaacaatgc aaagaggcaa catgaactgt ggagcttttc aagcccatca
     5881 gatgagactg gctcagaatg ctgccagaat accagggata cccaggcaca gcggccctca
     5941 atattccatg atgcagccac acctccaaag acaacactca aacccagggc atgctggacc
     6001 ctttcccgta gtatcggtac acaacaccac aatcaaccca acgagcccta ctacagcaac
     6061 tatggcaaat gcaaaccgag gtcccaccag cccatctgtt acagcaatag agctaatccc
     6121 ctcagttacc aatccagaaa accttccatc gctgccagat attccaccca tacagttgga
     6181 agatgctggc tcaagtagtt tagataatct actaagtaga tacatctcag gcagtcacct
     6241 acccccacag cctacaagca ccatgaatcc ttctccaggt ccctctgccc tttctccggg
     6301 atcatcaggt ttatccaatt ctcacacacc tgtgagaccc ccaagtactt ctagtactgg
     6361 cagtcgaggc agctgtgggt catcaggaag aactgctgag aagacaagtc ttagtttcaa
     6421 atctgatcag gtgaaggtca agcaagaacc tgggactgaa gatgaaatat gtagcttttc
     6481 aggaggtgta aaacaagaaa aaacagagga tggcaggagg agtgcttgca tgttgagcag
     6541 tcctgagagt agcttgacac cacctctctc aaccaacctg catctagaaa gtgaattgga
     6601 tgcattggca agcctggaaa accatgtgaa aattgaacct gcagatatga atgaaagctg
     6661 caaacagtca gggctcagca gccttgttaa tggaaagtcc ccaattcgaa gcctcatgca
     6721 caggtcggca aggattggag gagatggcaa caataaagat gatgacccaa atgaagactg
     6781 gtgtgctgtc tgccaaaacg gaggagatct cttgtgctgc gaaaaatgtc caaaggtctt
     6841 tcatctaact tgtcatgttc caacactact tagctttcca agtggggact ggatatgcac
     6901 attttgtaga gatattggaa agccagaagt tgaatatgat tgtgataatt tgcaacatag
     6961 taagaagggg aaaactgcgc aggggttaag ccccgtggac caaaggaaat gtgaacgtct
     7021 tctgctttac ctctattgcc atgaattaag tattgaattc caggagcctg ttcctgcttc
     7081 gataccaaac tactataaaa ttataaagaa accaatggat ttatccaccg tgaaaaagaa
     7141 gcttcagaaa aaacattccc aacactacca aatcccggat gactttgtgg ccgatgtccg
     7201 tttgatcttc aagaactgtg aaaggtttaa tgaagctgat tcagaagtag ctcaggcagg
     7261 gaaagcagtt gcattgtact ttgaagataa actcacagag atctactcag acaggacctt
     7321 cgcacctttg ccagagtttg agcaggaaga ggatgatggt gaggtaactg aggactctga
     7381 tgaagacttt atacagcccc gcagaaaacg cctaaagtca gatgagagac cagtacatat
     7441 aaagtagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     7501 ttgtggaaag gacgagcgac taacacttgt agcagtttaa cgcgttaagt cgacaatcaa
     7561 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     7621 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     7681 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7741 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7801 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7861 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7921 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     7981 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     8041 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     8101 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     8161 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8221 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     8281 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     8341 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     8401 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     8461 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     8521 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     8581 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8641 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     8701 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     8761 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     8821 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     8881 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     8941 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     9001 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     9061 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     9121 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     9181 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     9241 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     9301 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     9361 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     9421 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     9481 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     9541 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     9601 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     9661 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     9721 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     9781 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     9841 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     9901 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     9961 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
    10021 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
    10081 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
    10141 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
    10201 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
    10261 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
    10321 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
    10381 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
    10441 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
    10501 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
    10561 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
    10621 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
    10681 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
    10741 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
    10801 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
    10861 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
    10921 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
    10981 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
    11041 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
    11101 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
    11161 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
    11221 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
    11281 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
    11341 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
    11401 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
    11461 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
    11521 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
    11581 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
    11641 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagc
//
