LOCUS       sequence-407886-       12889 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12889)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 12889)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0681
FEATURES             Location/Qualifiers
     source          1..12889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        5..231
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             232..412
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    459..584
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1077..1310
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1495..1539
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        1770..1980
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        1792..1988
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      1839..1974
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2020..2622
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(2020..2039)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     2516..2536
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    2665..2782
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2920..4098
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3151..4089
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4046..4066
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             complement(5687..5698)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     misc_feature    8746..9334
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8799..8819)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9217..9228)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             9406..9639
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9717..9838
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9754..9773)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9808..9827
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9878..10013
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(10033..10052)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(10034..10052)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(10062..10079)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(10062..10078)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10071..10093)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10220..10675
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10307..10326)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10517..10538
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10701..10805
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10806..10874,10875..11666)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(11024..11043)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11837..12425
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12326..12345
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12579..12596
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12713..12734
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12749..12754,12755..12772,12773..12779)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12787..12803
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12792..12814
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12811..12827
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12811..12827
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12846..12866
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12848..12866
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat gagttagcaa
       61 catgccttac aaggagagaa aaagcaccgt gcatgccgat tggtggaagt aaggtggtac
      121 gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg aaccactgaa
      181 ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa cgggtctctc
      241 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
      301 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
      361 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtggcgcc
      421 cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc aggactcggc
      481 ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt
      541 tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt aagcggggga
      601 gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa aaatataaat
      661 taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat cctggcctgt
      721 tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc cttcagacag
      781 gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt gtgcatcaaa
      841 ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag caaaacaaaa
      901 gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg agatatgagg
      961 gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc attaggagta
     1021 gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt gggaatagga
     1081 gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc gtcaatgacg
     1141 ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa caatttgctg
     1201 agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat caagcagctc
     1261 caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct ggggatttgg
     1321 ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag ttggagtaat
     1381 aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag agaaattaac
     1441 aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca agaaaagaat
     1501 gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt taacataaca
     1561 aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt aggtttaaga
     1621 atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc accattatcg
     1681 tttcagaccc acctcccaac cccgagggga cccatgcatt gcatctcaat tagtcagcaa
     1741 ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc atgcgtctca
     1801 attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca
     1861 gttccgccca ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg
     1921 ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct
     1981 tttgcaaaaa gctttctaga ggtaccaccg gagcttacca tgaccgagta caagcccacg
     2041 gtgcgcctcg ccacccgcga cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc
     2101 gccgactacc ccgccacgcg ccacaccgtc gatccggacc gccacatcga gcgggtcacc
     2161 gagctgcaag aactcttcct cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg
     2221 gacgacggcg ccgccgtggc ggtctggacc acgccggaga gcgtcgaagc gggggcggtg
     2281 ttcgccgaga tcggcccgcg catggccgag ttgagcggtt cccggctggc cgcgcagcaa
     2341 cagatggaag gcctcctggc gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc
     2401 gtcggcgtct cgcccgacca ccagggcaag ggtctgggca gcgccgtcgt gctccccgga
     2461 gtggaggcgg ccgagcgcgc cggggtgccc gccttcctgg agacctccgc gccccgcaac
     2521 ctccccttct acgagcggct cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga
     2581 ccgcgcacct ggtgcatgac ccgcaagccc ggtgccagtt aacgaattct agatcttgag
     2641 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     2701 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     2761 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcg
     2821 ccggctcgag gcctgcaggt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     2881 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     2941 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     3001 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     3061 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     3121 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     3181 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     3241 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     3301 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     3361 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     3421 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     3481 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     3541 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     3601 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     3661 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     3721 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     3781 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     3841 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     3901 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     3961 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     4021 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     4081 ccatttcagg tgtcgtgagg ctagcaccgc caccatgccc cgccgccgcc ccccggcttc
     4141 cggtgctgcg cagtttccgg agcggatcgc aacccggagt ccggatccga tcccgctctg
     4201 cacattccaa aggcagccgc gcgccgcccc ggtccagccg ccatgccgac tgttctttgt
     4261 tacattcgcc ggctgcgggc accgttggcg atcggagtca aaacccggct ggatttcccg
     4321 gagccgctcc gggatcgccc tgcgcgccgc ccgcccgccg gggtcttcgc cgccccggcc
     4381 cgcggccccg cggcccccgc cgccgggcgg cgtcgtcgcc gaggccccag gggatgtcgt
     4441 tatcccccgc cctcgggtac agcccatgcg ggtcgcacgg gggggtccct ggacccccaa
     4501 ccccgcgttt agagaagctg agtcctggtc ccagattggg aaccagaggg tcagtgagca
     4561 gctcttggaa acatctctag ggaatgaggt gtccgacact gagccactga gccctgcgag
     4621 tgcaggcctg cgacgcaatc ctgccctacc tcctggaccc tttgcacaaa acttttcctg
     4681 ggggaaccag gaaaatctgc ccccagccct ggggaagatt gcgaacggag gaggaactgg
     4741 ggcaggcaag gccgaatgcg gctatgaaac tgagtcacac ttgctagagc cgcacgagat
     4801 acctttgaac gtgaatacac acaagttcag tgactgtgag tttccatatg agttttgcac
     4861 ggtctgcttt tcacccttca agctgctggg gatgagcggg gtggagggcg tgtggaatca
     4921 gcattcaaga agtgccagca tgcacacttt cctaaaccac tcagcaacgg gcatcagaga
     4981 agcaggttgc aggaaggaca tgccggtgtc agagatggct gaagatggga gcgaagagat
     5041 catgttcatc tggtgtgaag actgcagcca gtaccacgac tccgaatgtc ccgagctggg
     5101 cccagtggtc atggtcaaag actcctttgt gttaagcagg gcaaggtctt ggcctgccag
     5161 cggacacgtg cacacccagg cggggcaggg gatgcggggt tatgaggaca gggacagggc
     5221 tgacccacag cagcttccag aagcagtccc tgcaggcctg gtgaggcggc tcagtgggca
     5281 gcagctgccc tgccgttcca ccctcacctg ggggaggctg tgccacctgg tggcccaggg
     5341 caggtcatcc cttcctccca acttggagat cagacgactg gaagatggag ccgagggggt
     5401 gttcgccatc actcagctcg tcaagcggac acagttcggt ccctttgagt ccaggagggt
     5461 cgccaaatgg gaaaaggagt ctgcatttcc cctgaaggtg ttccagaagg acgggcaccc
     5521 cgtgtgcttc gacacctcca acgaggatga ctgcaactgg atgatgctgg tgcggccagc
     5581 ggcggaggcc gagcaccaga acctgacggc ctaccagcac ggcagcgacg tgtacttcac
     5641 cacctccaga gacatccccc cgggtaccga gctgcgcgtg tggtatgcgg ccttctatgc
     5701 caagaagatg gacaagccca tgctgaagca ggccggctct ggcgtccacg ctgcaggcac
     5761 cccagaaaac agcgcccccg tggagtcgga gcccagccag tgggcgtgta aagtgtgttc
     5821 tgccaccttc ctggagctgc agctcctcaa tgaacatctg ttgggccact tagaacaagc
     5881 caaaagcctt cctccaggca gccaaagcga ggcagcagct cccgagaagg agcaggacac
     5941 accccggggg gaaccccctg cagtgcccga gagcgagaat gttgccacca aagaacagaa
     6001 gaaaaagcct cgaaggggga gaaaacccaa agtgtccaaa gctgagcagc ctctagtcat
     6061 cgtggaagac aaggaaccca cagagcaagt ggcagagatc attaccgagg tccctccgga
     6121 tgagcctgtg agtgcaacgc cagatgagcg gatcatggag ctggttctgg ggaagctggc
     6181 caccaccacc actgacacca gctcggttcc aaagttcacc catcatcaga ataacaccat
     6241 cacgctcaag aggagcttaa ttctctcaag cagacacggc atccggcgca agctcatcaa
     6301 acagctcggg gagcacaagc gggtttacca gtgcaatatc tgcagcaaga tcttccagaa
     6361 cagcagcaac ctgagcaggc acgtgcgctc gcatggtgac aagctgttta agtgcgaaga
     6421 gtgtgcaaaa ttgttcagcc gcaaagagag cctaaagcag cacgtttcct acaagcacag
     6481 caggaacgag gtggacggcg agtacaggta ccgctgcggc acttgtgaga agaccttccg
     6541 catcgagagc gcgctggagt tccacaactg caggacagat gacaagacgt tccaatgtga
     6601 gatgtgtttc agattcttct ccaccaacag caacctctcc aagcacaaga agaagcacgg
     6661 cgacaagaag tttgcctgtg aggtctgcag caagatgttc taccgcaagg acgtcatgct
     6721 ggaccaccag cgccggcacc tggaaggagt gcggcgagtg aagcgagagg acctggaggc
     6781 cggtggggag aacctggtcc gttacaagaa ggagccttcc gggtgcccgg tgtgtggcaa
     6841 ggtgttctcc tgccggagca atatgaacaa gcacctgctc acccacggcg acaagaagta
     6901 cacctgcgag atctgcgggc gcaagttctt ccgcgtggat gtgctcaggg accacatcca
     6961 tgtccacttc aaggacatcg cgttgatgga tgaccaccag agggaagagt ttatcggcaa
     7021 gatcgggatc tcctcggaag aaaacgatga caattctgac gagagcgcag actcggagcc
     7081 tcacaagtac agctgcaagc ggtgccagct caccttcggc cgggggaagg agtacctgaa
     7141 gcacatcatg gaggtgcaca aggagaaggg ctatggctgc agcatctgca accggcgctt
     7201 tgcactgaag gccacctacc acgcccacat ggtcatccac cgtgaaaacc tgccggaccc
     7261 caacgtgcag aagtacatcc acccctgcga gatctgcggg cggatcttca acagcatcgg
     7321 gaacctggag cgccacaagc tcatccacac aggtgtgaag agccacgcct gcgagcagtg
     7381 tgggaagtcc tttgccagga aggacatgct gaaggagcac atgcgtgtgc acgacaatgt
     7441 ccgcgagtac ctgtgtgccg agtgtgggaa aggcatgaag accaagcacg cgctgcgcca
     7501 ccacatgaag ctgcacaagg gcatcaagga gtacgagtgc aaggagtgcc accgcaggtt
     7561 cgcgcagaag gtcaacatgc tcaagcactg caagcggcac acggggatta aagatttcat
     7621 gtgtgaattg tgtgggaaga cattcagcga gaggaacacc atggagaccc acaagctcat
     7681 ccacacagtg ggcaagcagt ggacgtgctc cgtgtgcgac aagaagtacg tgaccgagta
     7741 catgctgcag aagcacgttc agctcacaca cgacaaggtg gaggcgcaga gctgccagct
     7801 gtgcgggacc aaggtgtcca ccagggcctc catgagccga cacatgcggc gcaagcaccc
     7861 cgaggtgctc gcggtgagga tcgatgacct ggaccacctc ccggagacca ccaccatcga
     7921 cgcctcctcc attggcatcg tccagcctga gctgactctg gagcaggagg atttggccga
     7981 agggaagcac gggaaagctg ccaagcgaag tcacaagaga aagcagaagc cagaagagga
     8041 ggcgggtgct ccggtgcccg aggacgccac cttcagcgaa tactcagaga aagagacgga
     8101 gttcacaggc agtgtaggcg acgagaccaa ttccgcagta cagagcattc agcaggtagt
     8161 ggtgaccctg ggtgacccaa atgtgaccac accatcgagc tcagtcggct taaccaacat
     8221 caccgtgacc cccatcacca ctgcggccgc gactcagttt accaatctcc agccggtggc
     8281 cgtggggcac cttaccaccc ctgaacgcca gttacagctg gacaactcaa tcctgaccgt
     8341 gacctttgat accgtcagcg gctctgccat gttgcacaac cgccaaaatg acgtccagat
     8401 ccacccccag ccggaagcct cgaacccaca gtctgtggcc catttcatca acctgacgac
     8461 cctggtcaac tccatcacgc ccctggggag ccagcttagt gaccagcacc cgctcacgtg
     8521 gcgggcagtg ccccagactg acgtcttgcc accctcgcag ccgcaggcac ccccacagca
     8581 ggcggcccag ccccaggtgc aggcggagca gcagcagcag cagatgtaca gctactagta
     8641 atgaactagt ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa
     8701 ggacgaccat gaggtaggcc aagtgaccca acgcgttaag tcgacaatca acctctggat
     8761 tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt tacgctatgt
     8821 ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc tttcattttc
     8881 tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc cgttgtcagg
     8941 caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg gggcattgcc
     9001 accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc cacggcggaa
     9061 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     9121 tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg tgttgccacc
     9181 tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc agcggacctt
     9241 ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag
     9301 acgagtcgga tctccctttg ggccgcctcc ccgcgtcgac tttaagacca atgacttaca
     9361 aggcagctgt agatcttagc cactttttaa aagaaaaggg gggactggaa gggctaattc
     9421 actcccaacg aagacaagat ctgctttttg cttgtactgg gtctctctgg ttagaccaga
     9481 tctgagcctg ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct
     9541 tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat
     9601 ccctcagacc cttttagtca gtgtggaaaa tctctagcag tacgtatagt agttcatgtc
     9661 atcttattat tcagtattta taacttgcaa agaaatgaat atcagagagt gagaggaact
     9721 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata
     9781 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc
     9841 atgtctggct ctagctatcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     9901 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     9961 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctagggac
    10021 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
    10081 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
    10141 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
    10201 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
    10261 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
    10321 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
    10381 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
    10441 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
    10501 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
    10561 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
    10621 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
    10681 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
    10741 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
    10801 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
    10861 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
    10921 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
    10981 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
    11041 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
    11101 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
    11161 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
    11221 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
    11281 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
    11341 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
    11401 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
    11461 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
    11521 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
    11581 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
    11641 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
    11701 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
    11761 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
    11821 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
    11881 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
    11941 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
    12001 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
    12061 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
    12121 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
    12181 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
    12241 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
    12301 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
    12361 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
    12421 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
    12481 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
    12541 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
    12601 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
    12661 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
    12721 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
    12781 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
    12841 agcgcgcaat taaccctcac taaagggaac aaaagctgga gctgcaagc
//
