LOCUS       sequence-407901-        1203 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of SPAX8-related missense mutation (R200W) in 
            human NKX6-2 fused with the Venus fluorescent protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Ferreira-Peralta P, Franca B, Murtinheira F, Rodrigues MS, Herrera F
  TITLE     Is spastic ataxia 8 a protein misfolding disorder?
  JOURNAL   Biochim Biophys Acta Mol Basis Dis. 2023 Sep 9;1870(1):166882. doi: 
            10.1016/j.bbadis.2023.166882.
  PUBMED    37696463
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta Mol Basis Dis."; date: "2023-09-9"; volume: 
            "1870(1):166882. doi"; pages: " 10.1016/j.bbadis.2023.166882"
COMMENT     Sequence Label: pcDNA3.1 NKX6-2-Venus R200W
FEATURES             Location/Qualifiers
     source          1..1203
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(769..788)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 agcaggggga gttctggata agaaggcaag aagaagccca gcagacccac cttcagcggc
       61 cagcagatct ttgccctgga aaagaccttc gagcagacca agtatctggc tggccctgag
      121 agagccagac tggcctattc tctgggcatg accgagagcc aagtgaaagt gtggttccag
      181 aacagatgga ccaagtggcg gaagagacat gccgtggaaa tggccagcgc caagaagaaa
      241 caggacagcg acgccgagaa gctgaaagtt ggaggctccg atgccgagga cgacgacgag
      301 tataatagac ctctggaccc caacagcgac gatgagaaga tcacccggct gctgaagaaa
      361 cacaagccca gcaatctggc cctggtgtct ccttgtggtg gcggagctgg ggatgcactt
      421 ggatccggcg gaggtggaag cggcggaggc ggatctggta ccatggtttc taagggcgaa
      481 gaactgttca ccggcgtggt gcctatcctg gtggaactgg atggggatgt gaacggccac
      541 aagttctctg tgtctggcga aggcgaaggg gatgccacat acggcaagct gaccctgaag
      601 ctgatctgca ccacaggcaa actgcccgtg ccttggccta cactggtcac aacactcggc
      661 tacggcctgc agtgctttgc cagatatccc gaccacatga agcagcacga tttcttcaag
      721 agcgccatgc ctgagggcta cgtgcaagag cggaccatct tcttcaagga cgacggcaac
      781 tacaagacca gggccgaagt gaagttcgag ggcgacaccc tggtcaacag aatcgagctg
      841 aagggcatcg actttaaaga ggacggcaac atcctgggcc acaagctcga gtacaactac
      901 aacagccaca acgtgtacat caccgccgac aagcagaaga acggcatcaa ggccaacttc
      961 aagatccggc acaacatcga ggatggcggc gtgcagctgg ccgatcacta tcagcagaac
     1021 acccctatcg gagatggccc cgtcctgctg cctgataacc actacctgag ctaccagtct
     1081 gccctgagca aggaccccaa tgagaagagg gatcacatgg tgctgctgga attcgtgaca
     1141 gccgccggaa tcacactcgg catggacgag ctgtacaaat gaggactcct aaggcccgta
     1201 ccc
//
