LOCUS       sequence-407903-        1593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human NKX6-2 T252D phosphoresistant mutant.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1593)
  AUTHORS   Ferreira-Peralta P, Franca B, Murtinheira F, Rodrigues MS, Herrera F
  TITLE     Is spastic ataxia 8 a protein misfolding disorder?
  JOURNAL   Biochim Biophys Acta Mol Basis Dis. 2023 Sep 9;1870(1):166882. doi: 
            10.1016/j.bbadis.2023.166882.
  PUBMED    37696463
REFERENCE   2  (bases 1 to 1593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta Mol Basis Dis."; date: "2023-09-9"; volume: 
            "1870(1):166882. doi"; pages: " 10.1016/j.bbadis.2023.166882"
COMMENT     Sequence Label: pcDNA3.1 NKX6-2-Venus S246A/T252A
FEATURES             Location/Qualifiers
     source          1..1593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(1180..1199)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggacacaa acagacctgg cgcctttgtg ctgtctagcg ctcctctggc cgctctgcac
       61 aatatggccg agatgaagac cagcctgttt ccatacgctc tgcagggacc tgccggcttt
      121 aaggctcctg ctcttggagg actgggagca caactgcctc tgggaacacc tcacggcatc
      181 agcgatattc tgggcagacc tgttggagct gctggcggag gattgctcgg aggactgcct
      241 agactgaatg gactggcttc ttctgccggc gtgtactttg gacctgctgc tgccgttgcc
      301 agaggctatc ctaaacctct ggctgagctt cccggcagac ctcctatttt ttggcctggt
      361 gttgtgcagg gcgctccttg gagagatcct agacttgctg gaccagctcc tgcaggcgga
      421 gtgctggata aggatggcaa gaagaagcac agcagaccca ccttcagcgg ccagcagatc
      481 tttgccctgg aaaagacctt cgagcagacc aagtatctgg ctggccctga gagagccaga
      541 ctggcctatt ctctgggcat gaccgagagc caagtgaaag tgtggttcca gaacagacgg
      601 accaagtggc ggaagagaca tgccgtggaa atggccagcg ccaagaagaa acaggacagc
      661 gacgccgaga agctgaaagt tggaggctcc gatgccgagg acgacgacga gtataataga
      721 cctctggacc ccaacgccga cgatgagaag atcgcccggc tgctgaagaa acacaagccc
      781 agcaatctgg ccctggtgtc tccttgtggt ggcggagctg gggatgcact tggatccggc
      841 ggaggtggaa gcggcggagg cggatctggt accatggttt ctaagggcga agaactgttc
      901 accggcgtgg tgcctatcct ggtggaactg gatggggatg tgaacggcca caagttctct
      961 gtgtctggcg aaggcgaagg ggatgccaca tacggcaagc tgaccctgaa gctgatctgc
     1021 accacaggca aactgcccgt gccttggcct acactggtca caacactcgg ctacggcctg
     1081 cagtgctttg ccagatatcc cgaccacatg aagcagcacg atttcttcaa gagcgccatg
     1141 cctgagggct acgtgcaaga gcggaccatc ttcttcaagg acgacggcaa ctacaagacc
     1201 agggccgaag tgaagttcga gggcgacacc ctggtcaaca gaatcgagct gaagggcatc
     1261 gactttaaag aggacggcaa catcctgggc cacaagctcg agtacaacta caacagccac
     1321 aacgtgtaca tcaccgccga caagcagaag aacggcatca aggccaactt caagatccgg
     1381 cacaacatcg aggatggcgg cgtgcagctg gccgatcact atcagcagaa cacccctatc
     1441 ggagatggcc ccgtcctgct gcctgataac cactacctga gctaccagtc tgccctgagc
     1501 aaggacccca atgagaagag ggatcacatg gtgctgctgg aattcgtgac agccgccgga
     1561 atcacactcg gcatggacga gctgtacaaa tga
//
