LOCUS       sequence-407909-        1350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of human NKX6-2 cDNA SPAX8-related truncated 
            mutation containing the amino acids 1 to 196; fused with the green 
            fluorescent protein Venus allowing the use of fluorescent based 
            protocols..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1350)
  AUTHORS   Ferreira-Peralta P, Franca B, Murtinheira F, Rodrigues MS, Herrera F
  TITLE     Is spastic ataxia 8 a protein misfolding disorder?
  JOURNAL   Biochim Biophys Acta Mol Basis Dis. 2023 Sep 9;1870(1):166882. doi: 
            10.1016/j.bbadis.2023.166882.
  PUBMED    37696463
REFERENCE   2  (bases 1 to 1350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Biochim 
            Biophys Acta Mol Basis Dis."; date: "2023-09-9"; volume: 
            "1870(1):166882. doi"; pages: " 10.1016/j.bbadis.2023.166882"
COMMENT     Sequence Label: pcDNA3.1 NKX6-2(1-196)-Venus
FEATURES             Location/Qualifiers
     source          1..1350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(937..956)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atggacacaa acagacctgg cgcctttgtg ctgtctagcg ctcctctggc cgctctgcac
       61 aatatggccg agatgaagac cagcctgttt ccatacgctc tgcagggacc tgccggcttt
      121 aaggctcctg ctcttggagg actgggagca caactgcctc tgggaacacc tcacggcatc
      181 agcgatattc tgggcagacc tgttggagct gctggcggag gattgctcgg aggactgcct
      241 agactgaatg gactggcttc ttctgccggc gtgtactttg gacctgctgc tgccgttgcc
      301 agaggctatc ctaaacctct ggctgagctt cccggcagac ctcctatttt ttggcctggt
      361 gttgtgcagg gcgctccttg gagagatcct agacttgctg gaccagctcc tgcaggcgga
      421 gtgctggata aggatggcaa gaagaagcac agcagaccca ccttcagcgg ccagcagatc
      481 tttgccctgg aaaagacctt cgagcagacc aagtatctgg ctggccctga gagagccaga
      541 ctggcctatt ctctgggcat gaccgagagc caagtgaaag tgtggttcaa gcttggcgga
      601 ggtggaagcg gcggaggcgg atctggtacc atggtttcta agggcgaaga actgttcacc
      661 ggcgtggtgc ctatcctggt ggaactggat ggggatgtga acggccacaa gttctctgtg
      721 tctggcgaag gcgaagggga tgccacatac ggcaagctga ccctgaagct gatctgcacc
      781 acaggcaaac tgcccgtgcc ttggcctaca ctggtcacaa cactcggcta cggcctgcag
      841 tgctttgcca gatatcccga ccacatgaag cagcacgatt tcttcaagag cgccatgcct
      901 gagggctacg tgcaagagcg gaccatcttc ttcaaggacg acggcaacta caagaccagg
      961 gccgaagtga agttcgaggg cgacaccctg gtcaacagaa tcgagctgaa gggcatcgac
     1021 tttaaagagg acggcaacat cctgggccac aagctcgagt acaactacaa cagccacaac
     1081 gtgtacatca ccgccgacaa gcagaagaac ggcatcaagg ccaacttcaa gatccggcac
     1141 aacatcgagg atggcggcgt gcagctggcc gatcactatc agcagaacac ccctatcgga
     1201 gatggccccg tcctgctgcc tgataaccac tacctgagct accagtctgc cctgagcaag
     1261 gaccccaatg agaagaggga tcacatggtg ctgctggaat tcgtgacagc cgccggaatc
     1321 acactcggca tggacgagct gtacaaatga
//
